Rw7G004790

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001329
Physical Location & Seq
Reverse (-)
3955260 .. 3966908
11649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004790.1

Sequence Viewer

Length: 975 bp
ATGGCAGGAACCGTATTCACCATTCTCACATTCTGTTATTTGCAGGGCAGAAAAGAGAATATCAGTATCAAACCAAAATTGGGCGCAAGTTGTGCTGTTAATGCTATCATGTTGCTTGATATATTCCTCCACCCCACAGTTCCTGCGGTGCTGCTGGGGTTCTTTCTAATTGGGAAGGATATTCCAAGGATAATGGAGGGAAACATTCATGTCTCAGGATCTGGACACTTGGGCGGCACTGCAGTAGCAGCCATAGCCTGGGTACGACTTCGGATGGGGCTTAGGCCTCACAAAACCTTCTCCTTGACCAAACCACCCCAAAACCTCTTCTTTAGCTCCTTTCCCACACACCCACTTCAGCATCAACACAATCTCACTCAGAAAGTTCATGGCTTTTTCTCCAACCCATTACTCAGAAAGCACTTTTCTTTGGATTCCGCAAATACCCTTCTGAGAGTATCAAGCAAGAGGCTTTCGGATTGCAGGCTTGCCTTTCTCCAAAGAAAAATTCCCAAACAGAACTTTCAGTTCTTCCCAAGTTCTGATGCTCATCTGAGTGCTAGGAGATCATGGCCTGGGAGGGTATCCAGTAATGACGTGCTTTTGGGATTGATTATAGCTAATTTTGCTGTTTTTCTTTTATGGAAGATAGCAGATCCTGTATTCATGGTCAAGAATTTTACGCTCTCACTATACATTATCACAAGTGGACGTCTTCACACATTGATAACTTCTGCATTTAGTCACTTGGTTGTTGAGGATATTCTTCTCAACATGTTCCTACTCTCTGTCTTTGGGAAAGATGACGGAGACCTTTCAAACAGTCCCATATTGACATTGATGGCCTATCAAATTGGGGATTCCATTTCAAGGATCATACAGGGAAACAGTAGCTCAGGAAATGCACATGTGGGCGGCGCTGCAGTAGCAACTATTGCCTGGGTACGACTTCGGATGGGGCTTCGCGTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

324

Amino Acids

35.79

Weight (kDa)

10.46

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 715
AccB7I CCANNNNNTGG 1 cut(s) 258
AccII CGCG 1 cut(s) 966
AciI CCGC 4 cut(s) 146, 234, 438, 915
AclWI GGATC 3 cut(s) 226, 650, 881
AcsI RAATTY 2 cut(s) 507, 676
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 712
AfaI GTAC 2 cut(s) 264, 945
AfiI CCNNNNNNNGG 3 cut(s) 80, 258, 870
AflIII ACRYGT 2 cut(s) 774, 907
AgsI TTSAA 2 cut(s) 819, 870
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 3 cut(s) 257, 574, 938
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AluBI AGCT 3 cut(s) 336, 620, 894
AluI AGCT 3 cut(s) 336, 620, 894
Alw26I GTCTC 2 cut(s) 217, 804
AlwI GGATC 3 cut(s) 226, 650, 881
AlwNI CAGNNNCTG 3 cut(s) 143, 221, 659
AoxI GGCC 3 cut(s) 284, 572, 843
ApeKI GCWGC 3 cut(s) 151, 248, 920
ApoI RAATTY 2 cut(s) 507, 676
AspLEI GCGC 2 cut(s) 86, 920
AsuHPI GGTGA 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 707
BbvI GCAGC 3 cut(s) 138, 260, 907
BccI CCATC 3 cut(s) 268, 835, 949
BciT130I CCWGG 3 cut(s) 259, 576, 940
BciVI GTATCC 1 cut(s) 595
BcoDI GTCTC 2 cut(s) 217, 804
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 2 cut(s) 240, 921
BfoI RGCGCY 1 cut(s) 921
BfuI GTATCC 1 cut(s) 595
BisI GCNGC 5 cut(s) 152, 235, 249, 916, 921
BlsI GCNGC 5 cut(s) 153, 236, 250, 917, 922
Bme1390I CCNGG 3 cut(s) 259, 576, 940
BmgBI CACGTC 1 cut(s) 598
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 3 cut(s) 259, 576, 940
BmsI GCATC 2 cut(s) 370, 535
BpiI GAAGAC 1 cut(s) 707
Bpu10I CCTNAGC 2 cut(s) 281, 895
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 712
BsaI GGTCTC 1 cut(s) 804
BsaJI CCNNGG 4 cut(s) 185, 258, 575, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 258, 870
Bse1I ACTGG 1 cut(s) 588
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 3 cut(s) 259, 576, 940
BseDI CCNNGG 4 cut(s) 185, 258, 575, 939
BseGI GGATG 2 cut(s) 279, 960
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 3 cut(s) 80, 258, 870
BseMII CTCAG 6 cut(s) 228, 392, 427, 443, 545, 909
BseNI ACTGG 1 cut(s) 588
BseXI GCAGC 3 cut(s) 138, 260, 907
BseYI CCCAGC 1 cut(s) 154
Bsh1236I CGCG 1 cut(s) 966
BshFI GGCC 3 cut(s) 286, 574, 845
BslFI GGGAC 1 cut(s) 810
BslI CCNNNNNNNGG 3 cut(s) 80, 258, 870
BsmAI GTCTC 2 cut(s) 217, 804
BsmFI GGGAC 1 cut(s) 810
BsnI GGCC 3 cut(s) 286, 574, 845
Bso31I GGTCTC 1 cut(s) 804
Bsp143I GATC 4 cut(s) 218, 566, 655, 873
BspACI CCGC 4 cut(s) 146, 234, 438, 915
BspANI GGCC 3 cut(s) 286, 574, 845
BspCNI CTCAG 6 cut(s) 227, 391, 426, 444, 546, 908
BspFNI CGCG 1 cut(s) 966
BspLI GGNNCC 1 cut(s) 10
BspMAI CTGCAG 2 cut(s) 244, 925
BspPI GGATC 3 cut(s) 226, 650, 881
BspTNI GGTCTC 1 cut(s) 804
BsrI ACTGG 1 cut(s) 588
BssECI CCNNGG 4 cut(s) 185, 258, 575, 939
BssMI GATC 4 cut(s) 218, 566, 655, 873
BssNI GRCGYC 1 cut(s) 712
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 3 cut(s) 259, 576, 940
Bst4CI ACNGT 4 cut(s) 13, 139, 824, 890
Bst6I CTCTTC 1 cut(s) 332
BstACI GRCGYC 1 cut(s) 712
BstAPI GCANNNNNTGC 2 cut(s) 92, 935
BstC8I GCNNGC 2 cut(s) 485, 489
BstDEI CTNAG 7 cut(s) 214, 281, 378, 413, 452, 554, 895
BstF5I GGATG 2 cut(s) 279, 960
BstFNI CGCG 1 cut(s) 966
BstH2I RGCGCY 1 cut(s) 921
BstHHI GCGC 2 cut(s) 86, 920
BstKTI GATC 4 cut(s) 221, 569, 658, 876
BstMAI GTCTC 2 cut(s) 217, 804
BstMBI GATC 4 cut(s) 218, 566, 655, 873
BstMWI GCNNNNNNNGC 7 cut(s) 92, 101, 248, 254, 626, 926, 935
BstNI CCWGG 3 cut(s) 259, 576, 940
BstNSI RCATGY 2 cut(s) 778, 911
BstSCI CCNGG 3 cut(s) 257, 574, 938
BstSFI CTRYAG 2 cut(s) 240, 921
BstUI CGCG 1 cut(s) 966
BstV1I GCAGC 3 cut(s) 138, 260, 907
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 2 cut(s) 218, 655
BstYI RGATCY 2 cut(s) 218, 655
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 3 cut(s) 286, 574, 845
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 2 cut(s) 279, 960
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 485, 489
CaiI CAGNNNCTG 3 cut(s) 143, 221, 659
CfoI GCGC 2 cut(s) 86, 920
Csp6I GTAC 2 cut(s) 263, 944
CviAII CATG 7 cut(s) 109, 209, 389, 570, 667, 775, 908
CviQI GTAC 2 cut(s) 263, 944
DdeI CTNAG 7 cut(s) 214, 281, 378, 413, 452, 554, 895
DpnI GATC 4 cut(s) 220, 568, 657, 875
DpnII GATC 4 cut(s) 218, 566, 655, 873
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 185
Eco147I AGGCCT 1 cut(s) 286
Eco31I GGTCTC 1 cut(s) 804
Eco57I CTGAAG 1 cut(s) 341
EcoRII CCWGG 3 cut(s) 257, 574, 938
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 7 cut(s) 112, 212, 392, 573, 670, 778, 911
FaqI GGGAC 1 cut(s) 810
FatI CATG 7 cut(s) 108, 208, 388, 569, 666, 774, 907
Fnu4HI GCNGC 5 cut(s) 152, 235, 249, 916, 921
FokI GGATG 2 cut(s) 286, 967
Fsp4HI GCNGC 5 cut(s) 152, 235, 249, 916, 921
FspBI CTAG 1 cut(s) 561
GlaI GCGC 2 cut(s) 85, 919
GluI GCNGC 5 cut(s) 152, 235, 249, 916, 921
GsaI CCCAGC 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 921
HaeIII GGCC 3 cut(s) 286, 574, 845
HhaI GCGC 2 cut(s) 86, 920
Hin1I GRCGYC 1 cut(s) 712
Hin1II CATG 7 cut(s) 112, 212, 392, 573, 670, 778, 911
Hin6I GCGC 2 cut(s) 84, 918
HinP1I GCGC 2 cut(s) 84, 918
HinfI GANTC 2 cut(s) 434, 860
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 710
Hpy188I TCNGA 8 cut(s) 273, 381, 416, 453, 478, 544, 555, 954
Hpy188III TCNNGA 4 cut(s) 216, 222, 673, 897
Hpy8I GTNNAC 1 cut(s) 710
HpyAV CCTTC 3 cut(s) 169, 307, 458
HpyCH4III ACNGT 4 cut(s) 13, 139, 824, 890
HpyCH4IV ACGT 2 cut(s) 597, 712
HpyCH4V TGCA 6 cut(s) 43, 242, 483, 737, 905, 923
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 101, 248, 254, 626, 926, 935
HpyF3I CTNAG 7 cut(s) 214, 281, 378, 413, 452, 554, 895
HpySE526I ACGT 2 cut(s) 597, 712
Hsp92I GRCGYC 1 cut(s) 712
Hsp92II CATG 7 cut(s) 112, 212, 392, 573, 670, 778, 911
HspAI GCGC 2 cut(s) 84, 918
Kzo9I GATC 4 cut(s) 218, 566, 655, 873
LmnI GCTCC 1 cut(s) 341
Lsp1109I GCAGC 3 cut(s) 138, 260, 907
LweI GCATC 2 cut(s) 370, 535
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 597, 712
MaeIII GTNAC 1 cut(s) 743
MalI GATC 4 cut(s) 220, 568, 657, 875
MboI GATC 4 cut(s) 218, 566, 655, 873
MboII GAAGA 5 cut(s) 319, 523, 658, 707, 758
MflI RGATCY 2 cut(s) 218, 655
MluCI AATT 6 cut(s) 77, 168, 507, 622, 676, 852
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 7 cut(s) 137, 190, 297, 335, 462, 573, 751
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 555
MspR9I CCNGG 3 cut(s) 259, 576, 940
MvaI CCWGG 3 cut(s) 259, 576, 940
MvnI CGCG 1 cut(s) 966
MwoI GCNNNNNNNGC 7 cut(s) 92, 101, 248, 254, 626, 926, 935
NdeII GATC 4 cut(s) 218, 566, 655, 873
NlaIII CATG 7 cut(s) 112, 212, 392, 573, 670, 778, 911
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 743
NspI RCATGY 2 cut(s) 778, 911
PceI AGGCCT 1 cut(s) 286
PciI ACATGT 2 cut(s) 774, 907
PfeI GAWTC 2 cut(s) 434, 860
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 5 cut(s) 153, 236, 250, 917, 922
PscI ACATGT 2 cut(s) 774, 907
Psp6I CCWGG 3 cut(s) 257, 574, 938
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 3 cut(s) 257, 574, 938
PspN4I GGNNCC 1 cut(s) 10
PstI CTGCAG 2 cut(s) 244, 925
PstNI CAGNNNCTG 3 cut(s) 143, 221, 659
PsuI RGATCY 2 cut(s) 218, 655
RsaI GTAC 2 cut(s) 264, 945
RsaNI GTAC 2 cut(s) 263, 944
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 5 cut(s) 152, 235, 249, 916, 921
Sau3AI GATC 4 cut(s) 218, 566, 655, 873
ScrFI CCNGG 3 cut(s) 259, 576, 940
SetI ASST 8 cut(s) 299, 327, 338, 600, 622, 715, 816, 896
SfaNI GCATC 2 cut(s) 370, 535
SfcI CTRYAG 2 cut(s) 240, 921
SmiMI CAYNNNNRTG 1 cut(s) 555
Sse9I AATT 6 cut(s) 77, 168, 507, 622, 676, 852
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 4 cut(s) 146, 234, 438, 915
SspMI CTAG 1 cut(s) 561
StuI AGGCCT 1 cut(s) 286
StyD4I CCNGG 3 cut(s) 257, 574, 938
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 4 cut(s) 13, 139, 824, 890
TaiI ACGT 2 cut(s) 600, 715
TasI AATT 6 cut(s) 77, 168, 507, 622, 676, 852
TauI GCSGC 2 cut(s) 237, 918
TfiI GAWTC 2 cut(s) 434, 860
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 3 cut(s) 151, 248, 920
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 3 cut(s) 197, 377, 655
TspGWI ACGGA 1 cut(s) 822
TspRI CASTG 1 cut(s) 244
Van91I CCANNNNNTGG 1 cut(s) 258
XapI RAATTY 2 cut(s) 507, 676
XceI RCATGY 2 cut(s) 778, 911
XspI CTAG 1 cut(s) 561
ZraI GACGTC 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.