FvH4_6g27100

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001392
Physical Location & Seq
Reverse (-)
20794408 .. 20798012
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27100.t2

Sequence Viewer

Length: 2310 bp
ATGTCTTCTGAGCAACTTAGGAGGCAGAATGTGACGCCCGAGAGGGAAGTTCACATTGAGAAAGACAGGGTGCCCAAGATCACCAGCCACTTCGAGTCACTCACCGTGCATGTGCAAGAGGCCGGCGACAAAGAGACTCCAACGGACAGCACCAAGGACTCGCATGATGATCAGCAGAGGAAGGGAAACACCGTTGGCGACATGGGGATGGGAAAGTCCAGAGAGACTCACGAGCTGGAGTCTCATTCGAGACAGATGAAGGAGGAGAATGCTGAGAAGCAGAGAGAGAGGATGAGGGAGTCCCATGCGGATAGGGCTAGGACGGCGAATATAGTGGCAGATAAAGAAGCTGAGGAGAAAAGAGGGAGGGAGAGCGGTGCTCGTGGAGTAGGGATGTTTGAGATTCAAGAAAGTGATGAAGGTCAGAGGAAGGGAGGTAGAGGAGAAAGTAGAGAAGAAATGATTGTTGTGAGTCAAGGGAAGGAGGATTCGAGGGTTAGACAAGATAGAAGTGAAATGGAAGGCAGAAACATGGACATAATGGGTGGAGAGAGTAAGAGTGGAGCTCAAGGTGCCGGAAAAGTCGAAGTGATGAGGGTTGGGGAAGAGAAGGACAGAGAGGGTGAAGGCAGACAGCAAATGAGTTCTAAAGGAAGGCAGGGCGAGGGTAGACAAAGTGAAGGTGGGAGCACACAGATGATGGGCAAAGGACAGGGTATGAAACAAGAGAGAGCAACGGACACGGAGATGATGAGAAGCAAAGTGGAGGGTCGAGTAGGTTGCTCAGAGACTAGGAGCAGATCAGAAGAGGGTCGGGGCCGCGAGCAGAGACAAGGCACAGAAATGGAGAGCAGTGCGAACAAGCAGCAGCAACAGCAACAACAACCATCTCTAGACGAAGTTTCGGCATTTAGACAAACAGCACAGCAGAATTCCATGGAGGCAATAAGGGCTGCCGAAGAGCGATATCAGAAAACCAAAGAAAGTGCGAGCCAGACACTCGGTACTGCAGCCCAGAAGGCTAAAGAAGCATCTGAGAGAACTACAGAAACTGCAAGCCAGACACTTGGTACTGCAGCCCAGAGAGCTAAAGATACAGCCAGTACCGCTACCCAGAAGGCTAAAGAAATTTCAAGCCAGACACTTGGTACTGCAACTGAGAAAGGAGCACAAGTGAAAGACATTGCATTGGAGAAAGGCCAGCAAGGACTATATACAGCAAAAGACACTCTATCAAGTGCAGGGGCGAAAACAGCGGAGTATACGGTGCCACTAGCAGAGAAAGCTAAAGACATGGCTGTGTCTGCAGGTAAGACCACTCTGCATTATGCTGGTGATGTTGCGGTGGATTTGAAGGACAAGGTGACTGTGGCAGGTTGGACTGCGGCACAATACACAACTGAGGCAGCAGTGGAAGGAACCAAAACAGCCGCAAGAGTAGTTGCTGGAGCGGCGGAGTATGCTGGTCATAAGGCTGTAGATATTGTGTCCAAGCCATTGAGTGTTGCCAAAAATGCAGCTGCGGTGACTGGTGAGAGAGCTGAGGAGTTCACTGCTCGGAAGAAGGAAGAGGCACAGCGAGAATATGAAGCCAAGAAAGAAGCCGAAGCAAATAGGCCCAAGGGTTCCGAGACTACTTATCAAGGGGGAGAATCCAAGCAAGAGGAGACATGGACGAAACAAGTTGAGAAGGAATCTCAGCATATGCAGAGGGAAGGTGGGCAAGTAACTGAAAAAACCAGCCAAGAAACTGCTCAATCACAGAGGGAGGGAGTGCAAGAGGGTGGTTCTGGGGTGCTGGGAACAATCGGCGGGACTTTGGGTGTGATACCAGGAGCTGTTGGTGAGACCTTGGTTGAGATAGCTGAGAATACAAAAAACTTGGTTGTTGGGCAGGGTGGTAGTGGAATGGGTGAGGAGGGCCAGGAGGTGTGGAGCACAAATGCACGGCAAGGAGGGCAGCAGTGGAGCTCAAATGCTCAGGAAGGCAGGCAGGAAGGGAGGTCATCTGAGCAGCAAGGCAAGCAGGGTGAGACCTTGAGTGCAATTCCGGTAGCTGTTGGAGAGACTTTGGTTGAGATAACTCAGATTACAAGAGACAATGTCACTGGGCAGGGCCAAAGTGCAGAAGAAAGTGCTGAGATGGATTGGAGGTCATCTGATCAGCAGCAAGGCTTGGGTGCAATACCGCGTGCTGTTGGGGAGACTGTGGTTGGAATAGCTCAGACAACAAAAGACAACGTTGACAATCTTACTGGGCTTAGGCAGAGACAGGGACAGGGACAGGGACAGATGAGATCTGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

770

Amino Acids

83.23

Weight (kDa)

5.47

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1298, 1364
AccB1I GGYRCC 3 cut(s) 70, 572, 1267
AccBSI CCGCTC 2 cut(s) 375, 1451
AccI GTMKAC 2 cut(s) 670, 1262
AccII CGCG 2 cut(s) 822, 2191
AclI AACGTT 1 cut(s) 2241
AcsI RAATTY 2 cut(s) 931, 1128
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 4 cut(s) 1006, 1072, 1105, 1150
AgsI TTSAA 3 cut(s) 407, 1134, 1354
AjnI CCWGG 2 cut(s) 1831, 1923
Alw21I GWGCWC 6 cut(s) 382, 568, 692, 1171, 1940, 1973
AlwNI CAGNNNCTG 2 cut(s) 1052, 1838
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 6 cut(s) 120, 817, 1198, 1616, 1921, 2116
ApoI RAATTY 2 cut(s) 931, 1128
AspS9I GGNCC 4 cut(s) 817, 1617, 1921, 2116
AvaI CYCGRG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 75
BanI GGYRCC 3 cut(s) 70, 572, 1267
BanII GRGCYC 2 cut(s) 568, 1973
BauI CACGAG 2 cut(s) 230, 381
Bbv12I GWGCWC 6 cut(s) 382, 568, 692, 1171, 1940, 1973
BbvCI CCTCAGC 2 cut(s) 351, 1542
BccI CCATC 4 cut(s) 202, 694, 895, 2137
BceAI ACGGC 2 cut(s) 339, 1964
BcgI CGANNNNNNTGC 2 cut(s) 762, 796
BciT130I CCWGG 2 cut(s) 1833, 1925
BclI TGATCA 3 cut(s) 169, 2161, 2302
BfaI CTAG 4 cut(s) 318, 792, 893, 1274
BfmI CTRYAG 5 cut(s) 1008, 1044, 1074, 1305, 1476
BfuAI ACCTGC 2 cut(s) 1298, 1364
BglI GCCNNNNNGGC 1 cut(s) 1019
BglII AGATCT 1 cut(s) 2297
Bme1390I CCNGG 2 cut(s) 1833, 1925
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 4 cut(s) 817, 1617, 1921, 2116
BmiI GGNNCC 6 cut(s) 72, 574, 818, 1269, 1420, 1627
BmrFI CCNGG 2 cut(s) 1833, 1925
BmrI ACTGGG 2 cut(s) 2118, 2265
BmsI GCATC 1 cut(s) 1040
BmuI ACTGGG 2 cut(s) 2118, 2265
BplI GAGNNNNNCTC 4 cut(s) 364, 396, 550, 582
BpmI CTGGAG 2 cut(s) 257, 1467
Bpu10I CCTNAGC 4 cut(s) 351, 1542, 1980, 2261
BpuEI CTTGAG 2 cut(s) 552, 2059
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 2 cut(s) 1841, 2027
BsaJI CCNNGG 4 cut(s) 153, 936, 1620, 1851
BsaWI WCCGGW 1 cut(s) 2050
BsaXI ACNNNNNCTCC 2 cut(s) 1158, 1188
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 4 cut(s) 1101, 1534, 2113, 2260
Bse3DI GCAATG 1 cut(s) 1182
BseBI CCWGG 2 cut(s) 1833, 1925
BseDI CCNNGG 4 cut(s) 153, 936, 1620, 1851
BseGI GGATG 3 cut(s) 213, 297, 399
BseMI GCAATG 1 cut(s) 1182
BseNI ACTGG 4 cut(s) 1101, 1534, 2113, 2260
BseRI GAGGAG 6 cut(s) 278, 368, 456, 1559, 1679, 1931
BseSI GKGCMC 1 cut(s) 75
BseYI CCCAGC 1 cut(s) 1798
BsgI GTGCAG 2 cut(s) 1260, 2145
Bsh1236I CGCG 2 cut(s) 822, 2191
BshFI GGCC 6 cut(s) 122, 819, 1200, 1618, 1923, 2118
BshNI GGYRCC 3 cut(s) 70, 572, 1267
BsiHKAI GWGCWC 6 cut(s) 382, 568, 692, 1171, 1940, 1973
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 3 cut(s) 123, 576, 2051
BslFI GGGAC 5 cut(s) 286, 1828, 2289, 2295, 2301
BsmFI GGGAC 5 cut(s) 286, 1828, 2289, 2295, 2301
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 6 cut(s) 122, 819, 1200, 1618, 1923, 2118
Bso31I GGTCTC 2 cut(s) 1841, 2027
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 7 cut(s) 75, 382, 568, 692, 1171, 1940, 1973
Bsp143I GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
Bsp19I CCATGG 1 cut(s) 936
BspANI GGCC 6 cut(s) 122, 819, 1200, 1618, 1923, 2118
BspFNI CGCG 2 cut(s) 822, 2191
BspLI GGNNCC 6 cut(s) 72, 574, 818, 1269, 1420, 1627
BspMAI CTGCAG 3 cut(s) 1012, 1078, 1309
BspMI ACCTGC 2 cut(s) 1298, 1364
BspQI GCTCTTC 1 cut(s) 954
BspT107I GGYRCC 3 cut(s) 70, 572, 1267
BspTNI GGTCTC 2 cut(s) 1841, 2027
BsrBI CCGCTC 2 cut(s) 375, 1451
BsrDI GCAATG 1 cut(s) 1182
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 4 cut(s) 1101, 1534, 2113, 2260
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 153, 936, 1620, 1851
BssMI GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
BssNAI GTATAC 1 cut(s) 1263
BssNI GRCGYC 1 cut(s) 35
BssSI CACGAG 2 cut(s) 230, 381
BssT1I CCWWGG 4 cut(s) 153, 936, 1620, 1851
Bst1107I GTATAC 1 cut(s) 1263
Bst2BI CACGAG 2 cut(s) 230, 381
Bst2UI CCWGG 2 cut(s) 1833, 1925
Bst4CI ACNGT 5 cut(s) 106, 193, 1267, 1369, 2209
Bst6I CTCTTC 4 cut(s) 600, 801, 954, 1563
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 8 cut(s) 124, 824, 991, 1057, 1202, 1991, 2024, 2193
BstDSI CCRYGG 1 cut(s) 936
BstF5I GGATG 3 cut(s) 213, 297, 399
BstFNI CGCG 2 cut(s) 822, 2191
BstKTI GATC 6 cut(s) 81, 172, 803, 2164, 2300, 2305
BstMBI GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
BstNI CCWGG 2 cut(s) 1833, 1925
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 1831, 1923
BstSFI CTRYAG 5 cut(s) 1008, 1044, 1074, 1305, 1476
BstSLI GKGCMC 1 cut(s) 75
BstUI CGCG 2 cut(s) 822, 2191
BstX2I RGATCY 1 cut(s) 2297
BstXI CCANNNNNNTGG 2 cut(s) 1067, 1145
BstYI RGATCY 1 cut(s) 2297
BstZ17I GTATAC 1 cut(s) 1263
BsuRI GGCC 6 cut(s) 122, 819, 1200, 1618, 1923, 2118
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 3 cut(s) 213, 297, 399
BtsI GCAGTG 4 cut(s) 859, 1416, 1551, 1970
BtsIMutI CAGTG 5 cut(s) 859, 1416, 1551, 1970, 2106
BveI ACCTGC 2 cut(s) 1298, 1364
Cac8I GCNNGC 8 cut(s) 124, 824, 991, 1057, 1202, 1991, 2024, 2193
CaiI CAGNNNCTG 2 cut(s) 1052, 1838
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 817, 1617, 1921, 2116
CseI GACGC 1 cut(s) 43
Csp6I GTAC 4 cut(s) 1005, 1071, 1104, 1149
CviAII CATG 8 cut(s) 110, 164, 202, 305, 532, 937, 1294, 1671
CviQI GTAC 4 cut(s) 1005, 1071, 1104, 1149
DpnI GATC 6 cut(s) 80, 171, 802, 2163, 2299, 2304
DpnII GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
Eam1104I CTCTTC 4 cut(s) 600, 801, 954, 1563
EarI CTCTTC 4 cut(s) 600, 801, 954, 1563
EciI GGCGGA 1 cut(s) 1469
Ecl136II GAGCTC 2 cut(s) 566, 1971
Eco130I CCWWGG 4 cut(s) 153, 936, 1620, 1851
Eco24I GRGCYC 2 cut(s) 568, 1973
Eco31I GGTCTC 2 cut(s) 1841, 2027
Eco32I GATATC 1 cut(s) 968
Eco53kI GAGCTC 2 cut(s) 566, 1971
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 2 cut(s) 566, 1971
EcoRI GAATTC 1 cut(s) 931
EcoRII CCWGG 2 cut(s) 1831, 1923
EcoRV GATATC 1 cut(s) 968
EcoT14I CCWWGG 4 cut(s) 153, 936, 1620, 1851
EcoT38I GRGCYC 2 cut(s) 568, 1973
ErhI CCWWGG 4 cut(s) 153, 936, 1620, 1851
FaeI CATG 8 cut(s) 113, 167, 205, 308, 535, 940, 1297, 1674
FaqI GGGAC 5 cut(s) 286, 1828, 2289, 2295, 2301
FatI CATG 8 cut(s) 109, 163, 201, 304, 531, 936, 1293, 1670
FauI CCCGC 1 cut(s) 1805
FauNDI CATATG 1 cut(s) 1704
FbaI TGATCA 3 cut(s) 169, 2161, 2302
FblI GTMKAC 2 cut(s) 670, 1262
FokI GGATG 3 cut(s) 220, 304, 406
FriOI GRGCYC 2 cut(s) 568, 1973
FspBI CTAG 4 cut(s) 318, 792, 893, 1274
GsaI CCCAGC 1 cut(s) 1802
GsuI CTGGAG 2 cut(s) 257, 1467
HaeIII GGCC 6 cut(s) 122, 819, 1200, 1618, 1923, 2118
HapII CCGG 3 cut(s) 123, 576, 2051
HgaI GACGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 8 cut(s) 113, 167, 205, 308, 535, 940, 1297, 1674
HincII GTYRAC 1 cut(s) 2245
HindII GTYRAC 1 cut(s) 2245
HpaII CCGG 3 cut(s) 123, 576, 2051
Hpy166II GTNNAC 5 cut(s) 52, 671, 1263, 1551, 2245
Hpy188III TCNNGA 6 cut(s) 219, 230, 249, 407, 893, 1982
Hpy8I GTNNAC 5 cut(s) 52, 671, 1263, 1551, 2245
HpyCH4III ACNGT 5 cut(s) 106, 193, 1267, 1369, 2209
HpyCH4IV ACGT 1 cut(s) 2241
HpySE526I ACGT 1 cut(s) 2241
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 8 cut(s) 113, 167, 205, 308, 535, 940, 1297, 1674
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Ksp22I TGATCA 3 cut(s) 169, 2161, 2302
Kzo9I GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 8 cut(s) 563, 687, 795, 1166, 1448, 1835, 1935, 1968
LweI GCATC 1 cut(s) 1040
MaeI CTAG 4 cut(s) 318, 792, 893, 1274
MaeII ACGT 1 cut(s) 2241
MaeIII GTNAC 6 cut(s) 31, 96, 1363, 1525, 1726, 2104
MalI GATC 6 cut(s) 80, 171, 802, 2163, 2299, 2304
MbiI CCGCTC 2 cut(s) 375, 1451
MboI GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
MboII GAAGA 7 cut(s) 467, 617, 818, 971, 1573, 1580, 2141
MflI RGATCY 1 cut(s) 2297
MhlI GDGCHC 7 cut(s) 75, 382, 568, 692, 1171, 1940, 1973
MluCI AATT 3 cut(s) 931, 1128, 2046
MlyI GAGTC 7 cut(s) 104, 130, 152, 220, 248, 308, 481
MmeI TCCRAC 4 cut(s) 164, 1358, 2041, 2194
MroNI GCCGGC 1 cut(s) 122
MslI CAYNNNNRTG 5 cut(s) 206, 695, 746, 842, 1298
MspA1I CMGCKG 2 cut(s) 1256, 1520
MspI CCGG 3 cut(s) 123, 576, 2051
MspR9I CCNGG 2 cut(s) 1833, 1925
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 2 cut(s) 1833, 1925
MvnI CGCG 2 cut(s) 822, 2191
NaeI GCCGGC 1 cut(s) 124
NcoI CCATGG 1 cut(s) 936
NdeI CATATG 1 cut(s) 1704
NdeII GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 8 cut(s) 113, 167, 205, 308, 535, 940, 1297, 1674
NlaIV GGNNCC 6 cut(s) 72, 574, 818, 1269, 1420, 1627
NmuCI GTSAC 5 cut(s) 31, 96, 1363, 1525, 2104
NspI RCATGY 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 954
PctI GAATGC 1 cut(s) 274
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 4 cut(s) 403, 488, 1652, 1694
PflFI GACNNNGTC 1 cut(s) 2102
PleI GAGTC 7 cut(s) 103, 130, 152, 220, 247, 307, 480
PpsI GAGTC 7 cut(s) 103, 130, 152, 220, 247, 307, 480
Psp124BI GAGCTC 2 cut(s) 568, 1973
Psp1406I AACGTT 1 cut(s) 2241
Psp6I CCWGG 2 cut(s) 1831, 1923
PspFI CCCAGC 1 cut(s) 1798
PspGI CCWGG 2 cut(s) 1831, 1923
PspN4I GGNNCC 6 cut(s) 72, 574, 818, 1269, 1420, 1627
PspPI GGNCC 4 cut(s) 817, 1617, 1921, 2116
PstI CTGCAG 3 cut(s) 1012, 1078, 1309
PstNI CAGNNNCTG 2 cut(s) 1052, 1838
PsuI RGATCY 1 cut(s) 2297
PsyI GACNNNGTC 1 cut(s) 2102
PvuII CAGCTG 1 cut(s) 1520
RsaI GTAC 4 cut(s) 1006, 1072, 1105, 1150
RsaNI GTAC 4 cut(s) 1005, 1071, 1104, 1149
RseI CAYNNNNRTG 5 cut(s) 206, 695, 746, 842, 1298
SacI GAGCTC 2 cut(s) 568, 1973
SapI GCTCTTC 1 cut(s) 954
Sau3AI GATC 6 cut(s) 78, 169, 800, 2161, 2297, 2302
Sau96I GGNCC 4 cut(s) 817, 1617, 1921, 2116
SchI GAGTC 7 cut(s) 104, 130, 152, 220, 248, 308, 481
ScrFI CCNGG 2 cut(s) 1833, 1925
SduI GDGCHC 7 cut(s) 75, 382, 568, 692, 1171, 1940, 1973
SfaNI GCATC 1 cut(s) 1040
SfcI CTRYAG 5 cut(s) 1008, 1044, 1074, 1305, 1476
SmiMI CAYNNNNRTG 5 cut(s) 206, 695, 746, 842, 1298
SmlI CTYRAG 2 cut(s) 567, 2038
SmoI CTYRAG 2 cut(s) 567, 2038
Sse9I AATT 3 cut(s) 931, 1128, 2046
SspMI CTAG 4 cut(s) 318, 792, 893, 1274
SstI GAGCTC 2 cut(s) 568, 1973
StyD4I CCNGG 2 cut(s) 1831, 1923
StyI CCWWGG 4 cut(s) 153, 936, 1620, 1851
TaaI ACNGT 5 cut(s) 106, 193, 1267, 1369, 2209
TaiI ACGT 1 cut(s) 2244
TaqI TCGA 5 cut(s) 93, 248, 491, 585, 772
TasI AATT 3 cut(s) 931, 1128, 2046
TauI GCSGC 4 cut(s) 822, 1388, 1433, 1454
TfiI GAWTC 4 cut(s) 403, 488, 1652, 1694
TscAI CASTG 5 cut(s) 859, 1416, 1558, 1970, 2113
TseFI GTSAC 5 cut(s) 31, 96, 1363, 1525, 2104
Tsp45I GTSAC 5 cut(s) 31, 96, 1363, 1525, 2104
TspDTI ATGAA 4 cut(s) 272, 432, 734, 1602
TspGWI ACGGA 3 cut(s) 158, 752, 758
TspRI CASTG 5 cut(s) 859, 1416, 1558, 1970, 2113
Tth111I GACNNNGTC 1 cut(s) 2102
XapI RAATTY 2 cut(s) 931, 1128
XbaI TCTAGA 1 cut(s) 892
XceI RCATGY 1 cut(s) 113
XmiI GTMKAC 2 cut(s) 670, 1262
XspI CTAG 4 cut(s) 318, 792, 893, 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.