MD17G1233000.v1.1

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001392
Physical Location & Seq
Forward (+)
28134571 .. 28137185
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1233000.v1.1.491

Sequence Viewer

Length: 2280 bp
ATGGCTTCCGAGCAGCAGCAAAGAAGGGAAAACACAAAGCCGGAGAGGGAGGTGCACATAGAGAAAGAAAAGGTGCCCAAAATGACTACTCACTTTGAGGCTATGACGGTGCATGTTACTGATACGAGTGCTGGCAGAAAAGACACTCCTCTCGACAGCGGCAGCAAGCAGTCATCGAAGGACGAAGGCGATAAAGCAGCCTCGATGAGGAGAGGGAATACGGTGGGCGATGTGGAAATGGAGAAAGCAAGAGAGACTCATGAGATCGGGTCTCATAAGCTCGAGTCTCTTAAAGATACTCTGGCGTCGGCGAGGGAGGACAGACAGAGAGAATCGCAGGCAGACAAGGCGAGGGCAGAAAACATGGTGGCAGATAAAGAAGCTGAGTCTAAGAAAGAGAGCCGCCAACAAGGTGGACCTGGAATTCCTCAGTCTGAACAGAGCCATGAAGCTAAAAGTCAAGCCGGAGGTCAAGCTCATGGTCAACGTCAAGGTAGTGGCCCTCAGTTTGAACAGGGCCATGAAACTAAAAGCCATGGCGGAGTTCAAGGTGTAGCTCATTCATTTGAACAGCGCCATGAAGGTAAAGGTCAAGGTGGAGGTCAAGGTCAACGTCAAGGTGGAACTCGTCAATCTGTTGGGAAGGTTGAAGAGCGCCATGAAGCTCAAGGTCGCCAAACTACTGGGAAGGTTGAAGGGCTTCAAGGAGGTCAAGGACAAGCCCATGGTGGACGGCAAGGCCAATCTTTTGGGAAGGTTCAAGAGCGTCAAGGAGCCCATGGTGGAGGTCAACAACAACGAGAAGGCAGTGGTGGTGATCGCGGTCATCAGACGGTTGGAAAGTTTGAAGTGAGTGGTGAAGAAGAGCAGGGTCGGACTGATAAAGGCCGAGAAAGTAGTGAAGCTGATAAAGAATGTAGACGCACACAAATACACGAGGGTGTGGAGAAAATGACTCTTACCGGGGAAGACAAGAAGCTGCAGCTGGGCCAAGAGGAGGAGCAGCAAGGTTCTAAAGGAAGACGAGGCTCCGAGTCTGAGATGAAAAGCACACACGAGAGAAGCACTACTGAAGAGGAGACTAAGATAATCAAAGAAGAAGAGGGTCGACGTCAAGGAACCAAACAACAACAACCATCTTTGGAAGAAATCTCACAGCTGAGAGGAACAGCTCAGCAAAATTCTATGGAGGCGATGAGGGCTGCAGAGGAGCGTTACGCCAAGGCTAAAGAGTCGGCCAGGCAAGGGCTTGGCAGCGCAACCGAGTATGCCTCGGAGACAGCTGGACAAGCCAAAGACACTCTCCTCCAAGGAGCACAAACTGCCAAAGAGACTCTCACTAGTGCAGGTAAGACCGCCATCGAAAAAACAGCTCCACTAGCGGAGAGAGCCAAAGACGTTGCTGTCTCCGCCGGTCAGACCACTCTGCATTACGTGGAAGAAGGGGCTGTGAAGGCCAAGGACAAGACTTTGGAGGGCGGGAAGACTTCGGCGGCGTATGCAGGTGATGTGGCGGTGGAATTGAAGGACAAGGGGACTGTAGCCGGTTGGACGGCAGCGCATTACACCACAGAGGCTGCAGTGGAGGGGACTAAAAAGGCAGCGAAGGTTGTGATAGGGGCAGCTGAATATGCTGGGCATAAAGCAGTGGATATTGTTTCGAAGCCTTTGAGTGTGGTTAAAGATGCCGCTGCGGTGGCTGGGGAGAAGGCTGAGGAGTATACCGCGAGGAAGAAGGAGGAGGCGCAGAGAGTACTGGAGGCCAAGAGATCAGCTGAAGAGCAAAATAAGCAGCAGTCTCTCCAAGGAGGAGAAAGAGAAGGATCCGAATGGGAGCAAGGCACGACATGGAACAAGCAAGCTCATGAGAATGTTAGCGTTGGGATTCGAGGAGAGGGTGACCGATCATCCGAAAAAACTCAGGGTTATCAAGGTGGACAGGAATCCCAGAATCGATCTCAGGGCCTCATGCGAGGGGCAGACATGAGTCGGGATCAGTGCAGGGATGAGAGTTGGATGAAATCGCGGAGCGGTGGCGCTGGGGCAGGAAGCAACGAGGAGTACATGATGAGCATGGACAGCTCTCAAGTGGGAGAGGCTGGTGAGGGAGTGCTGGGAGCAGTCACGGAGACTTTACTGGAGATTGGTCAGACAACAAAAGACATGCTGATCGGCGGGCAGCACGGAATGGAGGAGGAGGAACTGAGTGCTGGCCAAGAACAGCAGTGGAAGTCGTCGACGGATGAACGGAGCAAGCAAGGGATGACCAAGAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

760

Amino Acids

81.96

Weight (kDa)

5.89

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1493
AasI GACNNNNNNGTC 1 cut(s) 1403
AatII GACGTC 1 cut(s) 1114
Acc36I ACCTGC 2 cut(s) 1337, 1493
AccB1I GGYRCC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 2031
AccI GTMKAC 4 cut(s) 919, 1108, 1721, 2237
AccII CGCG 3 cut(s) 822, 1727, 2026
AclWI GGATC 3 cut(s) 1818, 1831, 2001
AcoI YGGCCR 2 cut(s) 1236, 2212
AcsI RAATTY 2 cut(s) 423, 1180
AcuI CTGAAG 2 cut(s) 1092, 1797
AcyI GRCGYC 2 cut(s) 305, 1111
AfaI GTAC 2 cut(s) 1755, 2063
AfiI CCNNNNNNNGG 3 cut(s) 207, 997, 1245
AgsI TTSAA 9 cut(s) 512, 548, 569, 650, 695, 704, 761, 848, 1525
AhlI ACTAGT 1 cut(s) 1340
AjnI CCWGG 2 cut(s) 418, 1238
AleI CACNNNNGTG 1 cut(s) 939
Alw21I GWGCWC 2 cut(s) 57, 1318
Alw26I GTCTC 9 cut(s) 248, 276, 291, 1073, 1271, 1325, 1411, 1803, 2123
Alw44I GTGCAC 1 cut(s) 53
AlwI GGATC 3 cut(s) 1818, 1831, 2001
AlwNI CAGNNNCTG 1 cut(s) 1577
Ama87I CYCGRG 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 53
ApoI RAATTY 2 cut(s) 423, 1180
Asp700I GAANNNNTTC 1 cut(s) 699
AspLEI GCGC 6 cut(s) 576, 657, 1259, 1561, 1747, 2039
AspS9I GGNCC 5 cut(s) 416, 500, 517, 988, 1963
AsuC2I CCSGG 1 cut(s) 964
AsuHPI GGTGA 5 cut(s) 827, 869, 1517, 1910, 2114
AsuII TTCGAA 1 cut(s) 1661
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 2 cut(s) 57, 78
BalI TGGCCA 1 cut(s) 2214
BamHI GGATCC 1 cut(s) 1823
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 778
BauI CACGAG 2 cut(s) 935, 1055
BbsI GAAGAC 3 cut(s) 975, 1027, 1490
Bbv12I GWGCWC 2 cut(s) 57, 1318
BbvCI CCTCAGC 1 cut(s) 1713
BccI CCATC 2 cut(s) 1144, 1367
BceAI ACGGC 2 cut(s) 749, 1569
BciT130I CCWGG 2 cut(s) 420, 1240
BcnI CCSGG 1 cut(s) 964
BcoDI GTCTC 9 cut(s) 248, 276, 291, 1073, 1271, 1325, 1411, 1803, 2123
BcuI ACTAGT 1 cut(s) 1340
BfaI CTAG 2 cut(s) 1341, 1379
BfmI CTRYAG 4 cut(s) 980, 1203, 1539, 1578
BfoI RGCGCY 3 cut(s) 577, 658, 2040
BfuAI ACCTGC 2 cut(s) 1337, 1493
BlpI GCTNAGC 1 cut(s) 1173
BmcAI AGTACT 1 cut(s) 1755
Bme1390I CCNGG 3 cut(s) 420, 964, 1240
Bme18I GGWCC 1 cut(s) 416
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 5 cut(s) 416, 500, 517, 988, 1963
BmiI GGNNCC 5 cut(s) 75, 775, 1030, 1120, 1825
BmrFI CCNGG 3 cut(s) 420, 964, 1240
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 1 cut(s) 1675
BmuI ACTGGG 1 cut(s) 693
BoxI GACNNNNGTC 1 cut(s) 1986
BpiI GAAGAC 3 cut(s) 975, 1027, 1490
BplI GAGNNNNNCTC 2 cut(s) 1256, 1288
BpmI CTGGAG 2 cut(s) 1778, 2159
Bpu10I CCTNAGC 1 cut(s) 1713
Bpu1102I GCTNAGC 1 cut(s) 1173
Bpu14I TTCGAA 1 cut(s) 1661
BpuEI CTTGAG 2 cut(s) 651, 2070
BpuMI CCSGG 1 cut(s) 964
Bsa29I ATCGAT 1 cut(s) 1954
BsaAI YACGTR 1 cut(s) 1435
BsaHI GRCGYC 2 cut(s) 305, 1111
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 9 cut(s) 535, 724, 778, 963, 1221, 1272, 1309, 1458, 1804
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 997, 1245
Bse118I RCCGGY 2 cut(s) 1412, 1544
Bse1I ACTGG 3 cut(s) 688, 1761, 2142
BseBI CCWGG 2 cut(s) 420, 1240
BseCI ATCGAT 1 cut(s) 1954
BseDI CCNNGG 9 cut(s) 535, 724, 778, 963, 1221, 1272, 1309, 1458, 1804
BseGI GGATG 5 cut(s) 1907, 2011, 2022, 2248, 2268
BseLI CCNNNNNNNGG 3 cut(s) 207, 997, 1245
BseNI ACTGG 3 cut(s) 688, 1761, 2142
BseSI GKGCMC 2 cut(s) 57, 78
BseYI CCCAGC 5 cut(s) 985, 1634, 1700, 2039, 2113
BsgI GTGCAG 2 cut(s) 1365, 2020
Bsh1236I CGCG 3 cut(s) 822, 1727, 2026
BshNI GGYRCC 1 cut(s) 73
BshVI ATCGAT 1 cut(s) 1954
BsiHKAI GWGCWC 2 cut(s) 57, 1318
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 5 cut(s) 41, 465, 963, 1413, 1545
BslFI GGGAC 2 cut(s) 1549, 1603
BslI CCNNNNNNNGG 3 cut(s) 207, 997, 1245
BsmAI GTCTC 9 cut(s) 248, 276, 291, 1073, 1271, 1325, 1411, 1803, 2123
BsmFI GGGAC 2 cut(s) 1549, 1603
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 1661
Bsp1286I GDGCHC 4 cut(s) 57, 78, 778, 1318
Bsp143I GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
Bsp1720I GCTNAGC 1 cut(s) 1173
Bsp19I CCATGG 3 cut(s) 535, 724, 778
BspDI ATCGAT 1 cut(s) 1954
BspFNI CGCG 3 cut(s) 822, 1727, 2026
BspHI TCATGA 2 cut(s) 259, 1864
BspLI GGNNCC 5 cut(s) 75, 775, 1030, 1120, 1825
BspMAI CTGCAG 3 cut(s) 984, 1207, 1582
BspMI ACCTGC 2 cut(s) 1337, 1493
BspPI GGATC 3 cut(s) 1818, 1831, 2001
BspQI GCTCTTC 3 cut(s) 645, 858, 1773
BspT104I TTCGAA 1 cut(s) 1661
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 2031
BsrFI RCCGGY 2 cut(s) 1412, 1544
BsrI ACTGG 3 cut(s) 688, 1761, 2142
BssAI RCCGGY 2 cut(s) 1412, 1544
BssECI CCNNGG 9 cut(s) 535, 724, 778, 963, 1221, 1272, 1309, 1458, 1804
BssMI GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
BssNAI GTATAC 1 cut(s) 1722
BssNI GRCGYC 2 cut(s) 305, 1111
BssSI CACGAG 2 cut(s) 935, 1055
BssT1I CCWWGG 7 cut(s) 535, 724, 778, 1221, 1309, 1458, 1804
Bst1107I GTATAC 1 cut(s) 1722
Bst2BI CACGAG 2 cut(s) 935, 1055
Bst2UI CCWGG 2 cut(s) 420, 1240
Bst4CI ACNGT 4 cut(s) 109, 223, 835, 1540
Bst6I CTCTTC 5 cut(s) 645, 858, 1068, 1095, 1773
BstACI GRCGYC 2 cut(s) 305, 1111
BstAPI GCANNNNNTGC 1 cut(s) 1322
BstBAI YACGTR 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 1661
BstC8I GCNNGC 7 cut(s) 133, 167, 339, 1860, 2177, 2212, 2255
BstDSI CCRYGG 3 cut(s) 535, 724, 778
BstEII GGTNACC 1 cut(s) 1898
BstENI CCTNNNNNAGG 1 cut(s) 205
BstF5I GGATG 5 cut(s) 1907, 2011, 2022, 2248, 2268
BstFNI CGCG 3 cut(s) 822, 1727, 2026
BstH2I RGCGCY 3 cut(s) 577, 658, 2040
BstHHI GCGC 6 cut(s) 576, 657, 1259, 1561, 1747, 2039
BstKTI GATC 8 cut(s) 267, 820, 1772, 1826, 1907, 1958, 1996, 2172
BstMAI GTCTC 9 cut(s) 248, 276, 291, 1073, 1271, 1325, 1411, 1803, 2123
BstMBI GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
BstNI CCWGG 2 cut(s) 420, 1240
BstNSI RCATGY 2 cut(s) 116, 2167
BstPAI GACNNNNGTC 1 cut(s) 1986
BstPI GGTNACC 1 cut(s) 1898
BstSCI CCNGG 3 cut(s) 418, 962, 1238
BstSFI CTRYAG 4 cut(s) 980, 1203, 1539, 1578
BstSLI GKGCMC 2 cut(s) 57, 78
BstUI CGCG 3 cut(s) 822, 1727, 2026
BstV2I GAAGAC 3 cut(s) 975, 1027, 1490
BstX2I RGATCY 1 cut(s) 1823
BstXI CCANNNNNNTGG 3 cut(s) 413, 683, 749
BstYI RGATCY 1 cut(s) 1823
BstZ17I GTATAC 1 cut(s) 1722
Bsu15I ATCGAT 1 cut(s) 1954
BsuTUI ATCGAT 1 cut(s) 1954
BtgI CCRYGG 3 cut(s) 535, 724, 778
BtgZI GCGATG 2 cut(s) 243, 1208
BtsCI GGATG 5 cut(s) 1907, 2011, 2022, 2248, 2268
BtsI GCAGTG 4 cut(s) 814, 1587, 1653, 2231
BtsIMutI CAGTG 5 cut(s) 814, 1587, 1653, 2003, 2231
BveI ACCTGC 2 cut(s) 1337, 1493
Cac8I GCNNGC 7 cut(s) 133, 167, 339, 1860, 2177, 2212, 2255
CaiI CAGNNNCTG 1 cut(s) 1577
CciI TCATGA 2 cut(s) 259, 1864
CfoI GCGC 6 cut(s) 576, 657, 1259, 1561, 1747, 2039
Cfr10I RCCGGY 2 cut(s) 1412, 1544
Cfr13I GGNCC 5 cut(s) 416, 500, 517, 988, 1963
ClaI ATCGAT 1 cut(s) 1954
CseI GACGC 3 cut(s) 294, 755, 930
Csp6I GTAC 2 cut(s) 1754, 2062
CviQI GTAC 2 cut(s) 1754, 2062
DpnI GATC 8 cut(s) 266, 819, 1771, 1825, 1906, 1957, 1995, 2171
DpnII GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
DrdI GACNNNNNNGTC 1 cut(s) 1403
DseDI GACNNNNNNGTC 1 cut(s) 1403
EaeI YGGCCR 2 cut(s) 1236, 2212
Eam1104I CTCTTC 5 cut(s) 645, 858, 1068, 1095, 1773
EarI CTCTTC 5 cut(s) 645, 858, 1068, 1095, 1773
EciI GGCGGA 2 cut(s) 555, 1399
Eco130I CCWWGG 7 cut(s) 535, 724, 778, 1221, 1309, 1458, 1804
Eco24I GRGCYC 1 cut(s) 778
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 1092, 1797
Eco88I CYCGRG 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 1898
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 1963
EcoO65I GGTNACC 1 cut(s) 1898
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 418, 1238
EcoT14I CCWWGG 7 cut(s) 535, 724, 778, 1221, 1309, 1458, 1804
EcoT38I GRGCYC 1 cut(s) 778
ErhI CCWWGG 7 cut(s) 535, 724, 778, 1221, 1309, 1458, 1804
FaqI GGGAC 2 cut(s) 1549, 1603
FauI CCCGC 2 cut(s) 1472, 2168
FblI GTMKAC 4 cut(s) 919, 1108, 1721, 2237
FokI GGATG 5 cut(s) 1894, 2018, 2029, 2255, 2275
FriOI GRGCYC 1 cut(s) 778
FspBI CTAG 2 cut(s) 1341, 1379
GlaI GCGC 6 cut(s) 575, 656, 1258, 1560, 1746, 2038
GsaI CCCAGC 5 cut(s) 989, 1638, 1704, 2043, 2117
GsuI CTGGAG 2 cut(s) 1778, 2159
HaeII RGCGCY 3 cut(s) 577, 658, 2040
HapII CCGG 5 cut(s) 41, 465, 963, 1413, 1545
HgaI GACGC 3 cut(s) 294, 755, 930
HhaI GCGC 6 cut(s) 576, 657, 1259, 1561, 1747, 2039
Hin1I GRCGYC 2 cut(s) 305, 1111
Hin6I GCGC 6 cut(s) 574, 655, 1257, 1559, 1745, 2037
HinP1I GCGC 6 cut(s) 574, 655, 1257, 1559, 1745, 2037
HincII GTYRAC 5 cut(s) 485, 611, 791, 1109, 2238
HindII GTYRAC 5 cut(s) 485, 611, 791, 1109, 2238
HpaII CCGG 5 cut(s) 41, 465, 963, 1413, 1545
HphI GGTGA 5 cut(s) 827, 869, 1517, 1910, 2114
Hpy188III TCNNGA 5 cut(s) 152, 260, 761, 1865, 1991
Hpy99I CGWCG 4 cut(s) 310, 1113, 2239, 2242
HpyCH4III ACNGT 4 cut(s) 109, 223, 835, 1540
HpyCH4IV ACGT 5 cut(s) 487, 613, 1111, 1398, 1434
HpyCH4V TGCA 9 cut(s) 55, 112, 982, 1205, 1346, 1429, 1502, 1580, 2001
HpySE526I ACGT 5 cut(s) 487, 613, 1111, 1398, 1434
Hsp92I GRCGYC 2 cut(s) 305, 1111
HspAI GCGC 6 cut(s) 574, 655, 1257, 1559, 1745, 2037
Kzo9I GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
LguI GCTCTTC 3 cut(s) 645, 858, 1773
LweI GCATC 1 cut(s) 1675
MaeI CTAG 2 cut(s) 1341, 1379
MaeII ACGT 5 cut(s) 487, 613, 1111, 1398, 1434
MaeIII GTNAC 4 cut(s) 115, 1214, 1898, 2122
MalI GATC 8 cut(s) 266, 819, 1771, 1825, 1906, 1957, 1995, 2171
MbiI CCGCTC 1 cut(s) 2031
MboI GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
MflI RGATCY 1 cut(s) 1823
MhlI GDGCHC 4 cut(s) 57, 78, 778, 1318
MlsI TGGCCA 1 cut(s) 2214
MluCI AATT 4 cut(s) 423, 1180, 1520, 2275
MluNI TGGCCA 1 cut(s) 2214
MlyI GAGTC 8 cut(s) 250, 293, 395, 949, 1043, 1241, 1327, 1996
MmeI TCCRAC 4 cut(s) 817, 854, 1529, 1994
Mox20I TGGCCA 1 cut(s) 2214
MroXI GAANNNNTTC 1 cut(s) 699
MscI TGGCCA 1 cut(s) 2214
MseI TTAA 2 cut(s) 291, 1680
MslI CAYNNNNRTG 2 cut(s) 939, 1869
Msp20I TGGCCA 1 cut(s) 2214
MspA1I CMGCKG 7 cut(s) 159, 985, 1159, 1283, 1625, 1691, 1775
MspI CCGG 5 cut(s) 41, 465, 963, 1413, 1545
MspR9I CCNGG 3 cut(s) 420, 964, 1240
MvaI CCWGG 2 cut(s) 420, 1240
MvnI CGCG 3 cut(s) 822, 1727, 2026
NciI CCSGG 1 cut(s) 964
NcoI CCATGG 3 cut(s) 535, 724, 778
NdeII GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
NlaIV GGNNCC 5 cut(s) 75, 775, 1030, 1120, 1825
NmeAIII GCCGAG 1 cut(s) 914
NmuCI GTSAC 2 cut(s) 1898, 2122
NspI RCATGY 2 cut(s) 116, 2167
NspV TTCGAA 1 cut(s) 1661
OliI CACNNNNGTG 1 cut(s) 939
PaeR7I CTCGAG 1 cut(s) 281
PagI TCATGA 2 cut(s) 259, 1864
PaqCI CACCTGC 1 cut(s) 1493
PciSI GCTCTTC 3 cut(s) 645, 858, 1773
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 4 cut(s) 332, 1885, 1943, 1951
PleI GAGTC 8 cut(s) 250, 292, 394, 949, 1042, 1240, 1327, 1995
PpsI GAGTC 8 cut(s) 250, 292, 394, 949, 1042, 1240, 1327, 1995
Ppu21I YACGTR 1 cut(s) 1435
PshAI GACNNNNGTC 1 cut(s) 1986
Psp6I CCWGG 2 cut(s) 418, 1238
PspEI GGTNACC 1 cut(s) 1898
PspFI CCCAGC 5 cut(s) 985, 1634, 1700, 2039, 2113
PspGI CCWGG 2 cut(s) 418, 1238
PspN4I GGNNCC 5 cut(s) 75, 775, 1030, 1120, 1825
PspPI GGNCC 5 cut(s) 416, 500, 517, 988, 1963
PspXI VCTCGAGB 1 cut(s) 281
PstI CTGCAG 3 cut(s) 984, 1207, 1582
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 1 cut(s) 1823
PvuII CAGCTG 5 cut(s) 985, 1159, 1283, 1625, 1775
RsaI GTAC 2 cut(s) 1755, 2063
RsaNI GTAC 2 cut(s) 1754, 2062
RseI CAYNNNNRTG 2 cut(s) 939, 1869
SalI GTCGAC 2 cut(s) 1107, 2236
SapI GCTCTTC 3 cut(s) 645, 858, 1773
SaqAI TTAA 2 cut(s) 291, 1680
Sau3AI GATC 8 cut(s) 264, 817, 1769, 1823, 1904, 1955, 1993, 2169
Sau96I GGNCC 5 cut(s) 416, 500, 517, 988, 1963
ScaI AGTACT 1 cut(s) 1755
SchI GAGTC 8 cut(s) 250, 293, 395, 949, 1043, 1241, 1327, 1996
ScrFI CCNGG 3 cut(s) 420, 964, 1240
SduI GDGCHC 4 cut(s) 57, 78, 778, 1318
SfaNI GCATC 1 cut(s) 1675
SfcI CTRYAG 4 cut(s) 980, 1203, 1539, 1578
Sfr274I CTCGAG 1 cut(s) 281
SfuI TTCGAA 1 cut(s) 1661
SgrDI CGTCGACG 1 cut(s) 2236
SinI GGWCC 1 cut(s) 416
SlaI CTCGAG 1 cut(s) 281
SmiMI CAYNNNNRTG 2 cut(s) 939, 1869
SmlI CTYRAG 3 cut(s) 281, 666, 2085
SmoI CTYRAG 3 cut(s) 281, 666, 2085
SpeI ACTAGT 1 cut(s) 1340
Sse9I AATT 4 cut(s) 423, 1180, 1520, 2275
SspMI CTAG 2 cut(s) 1341, 1379
StyD4I CCNGG 3 cut(s) 418, 962, 1238
StyI CCWWGG 7 cut(s) 535, 724, 778, 1221, 1309, 1458, 1804
TaaI ACNGT 4 cut(s) 109, 223, 835, 1540
TaiI ACGT 5 cut(s) 490, 616, 1114, 1401, 1437
TaqII GACCGA 1 cut(s) 1917
TasI AATT 4 cut(s) 423, 1180, 1520, 2275
TatI WGTACW 2 cut(s) 1753, 2061
TauI GCSGC 4 cut(s) 162, 405, 1496, 1691
TfiI GAWTC 4 cut(s) 332, 1885, 1943, 1951
Tru1I TTAA 2 cut(s) 291, 1680
Tru9I TTAA 2 cut(s) 291, 1680
TscAI CASTG 5 cut(s) 814, 1587, 1653, 2003, 2231
TseFI GTSAC 2 cut(s) 1898, 2122
Tsp45I GTSAC 2 cut(s) 1898, 2122
TspDTI ATGAA 8 cut(s) 462, 537, 552, 594, 675, 1058, 2033, 2259
TspGWI ACGGA 4 cut(s) 2141, 2199, 2255, 2263
TspRI CASTG 5 cut(s) 814, 1587, 1653, 2003, 2231
VneI GTGCAC 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 416
XagI CCTNNNNNAGG 1 cut(s) 205
XapI RAATTY 2 cut(s) 423, 1180
XceI RCATGY 2 cut(s) 116, 2167
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 4 cut(s) 919, 1108, 1721, 2237
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 2 cut(s) 1341, 1379
ZraI GACGTC 1 cut(s) 1112
ZrmI AGTACT 1 cut(s) 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.