Rh2CG323200

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001392
Physical Location & Seq
Reverse (-)
41650992 .. 41651786
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG323200.1

Sequence Viewer

Length: 795 bp
ATGGTTATTTTCAGAGGCCACCAATTTCATAACTTCGTATACAAACTAATTAGTTTTTTGCTTGATGTGAACAAGGGTTCTGAGACTACTTATCAAGGAGGAGAATCCAAAAGCTGGGAGCAAGAGGAGACACGGACGAAACCAGTTGAGAGGGAATCTCAGCATATGCAGAGAGAAGGTGGCAGACCAACTGAAAAAACCAGCCAAGAAACCGCTGAATTACAAAGGGAGGGAGCGCAAGAGGGCGGTGCTGGGGTGCTGGAAACAATCGGCGGGACTTTGGGTGTGATACCGGGAGCTGTTAGTGAGACATTGGTTGAGATAGCTGAGAATACAAAAAACTTTGTTATTGGGCAGGGTGAAAGTGGAAAGGGTGAGGAGGGCCAGGAAGGGTGGAGTTCAAATGCACAGCAAGGAGGGCAGGGGGGGAGGTCATCTGAGAAGCAAGGCAGGCAAGAGGGGAGGTCATTTGAGCAGCAAGGCAAGCAGAGCGAGACTGTAAGTGTAATACCGGGAGCTGTCGGCGAAACTTTGATTGAGATAGCTCAGACTACAAAAGACAACGTCATCGGGCAGGGCCAAAGCGCAGAGGAGAGTGCTGAGAAGGTTCGCAGGTCATCTGATCAGCAGCAAGGCTTAGGTGCAATACCTCGTGCTGTTGGGGAGACTATGGTTGGAATAGCTCAGACAACAAAAGACAATGTTGACAATATTACTGGGCTTAGGCGGGGACGGGGACAGGGACAGTCGAGAACTGATCAGCAGCAGGGATGGAAGCAAGACCAGACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

28.55

Weight (kDa)

5.18

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 603
AccB7I CCANNNNNTGG 1 cut(s) 114
AccI GTMKAC 1 cut(s) 39
AciI CCGC 4 cut(s) 213, 246, 273, 727
AfiI CCNNNNNNNGG 1 cut(s) 114
AgsI TTSAA 1 cut(s) 402
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 6 cut(s) 114, 299, 326, 518, 545, 683
AluI AGCT 6 cut(s) 114, 299, 326, 518, 545, 683
Alw26I GTCTC 5 cut(s) 77, 122, 302, 488, 659
AoxI GGCC 3 cut(s) 16, 382, 577
ApeKI GCWGC 3 cut(s) 475, 628, 763
AspLEI GCGC 2 cut(s) 238, 587
AspS9I GGNCC 2 cut(s) 382, 577
AsuC2I CCSGG 2 cut(s) 294, 513
AsuHPI GGTGA 2 cut(s) 371, 386
BauI CACGAG 1 cut(s) 651
BbvI GCAGC 3 cut(s) 487, 640, 775
BccI CCATC 1 cut(s) 765
BciT130I CCWGG 1 cut(s) 386
BclI TGATCA 2 cut(s) 622, 757
BcnI CCSGG 2 cut(s) 294, 513
BcoDI GTCTC 5 cut(s) 77, 122, 302, 488, 659
BfuAI ACCTGC 1 cut(s) 603
BisI GCNGC 3 cut(s) 476, 629, 764
BlsI GCNGC 3 cut(s) 477, 630, 765
Bme1390I CCNGG 3 cut(s) 294, 386, 513
BmgT120I GGNCC 2 cut(s) 382, 577
BmrFI CCNGG 3 cut(s) 294, 386, 513
BmrI ACTGGG 1 cut(s) 726
BmuI ACTGGG 1 cut(s) 726
BplI GAGNNNNNCTC 2 cut(s) 142, 174
Bpu10I CCTNAGC 2 cut(s) 637, 722
BpuMI CCSGG 2 cut(s) 294, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 2 cut(s) 143, 721
BseBI CCWGG 1 cut(s) 386
BseGI GGATG 1 cut(s) 776
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMII CTCAG 7 cut(s) 72, 173, 318, 429, 560, 591, 698
BseNI ACTGG 2 cut(s) 143, 721
BseRI GAGGAG 4 cut(s) 114, 140, 392, 605
BseXI GCAGC 3 cut(s) 487, 640, 775
BseYI CCCAGC 2 cut(s) 114, 251
BshFI GGCC 3 cut(s) 18, 384, 579
BsiSI CCGG 2 cut(s) 293, 512
BslFI GGGAC 4 cut(s) 289, 744, 750, 756
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 5 cut(s) 77, 122, 302, 488, 659
BsmFI GGGAC 4 cut(s) 289, 744, 750, 756
BsnI GGCC 3 cut(s) 18, 384, 579
Bsp143I GATC 2 cut(s) 622, 757
BspACI CCGC 4 cut(s) 213, 246, 273, 727
BspANI GGCC 3 cut(s) 18, 384, 579
BspCNI CTCAG 7 cut(s) 73, 172, 319, 430, 559, 592, 697
BspMI ACCTGC 1 cut(s) 603
BsrI ACTGG 2 cut(s) 143, 721
BssMI GATC 2 cut(s) 622, 757
BssNAI GTATAC 1 cut(s) 40
BssSI CACGAG 1 cut(s) 651
Bst1107I GTATAC 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 2 cut(s) 499, 747
BstC8I GCNNGC 2 cut(s) 452, 485
BstDEI CTNAG 9 cut(s) 81, 159, 327, 438, 546, 600, 637, 684, 722
BstF5I GGATG 1 cut(s) 776
BstHHI GCGC 2 cut(s) 238, 587
BstKTI GATC 2 cut(s) 625, 760
BstMAI GTCTC 5 cut(s) 77, 122, 302, 488, 659
BstMBI GATC 2 cut(s) 622, 757
BstMWI GCNNNNNNNGC 4 cut(s) 418, 451, 484, 489
BstNI CCWGG 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 292, 384, 511
BstV1I GCAGC 3 cut(s) 487, 640, 775
BstZ17I GTATAC 1 cut(s) 40
BsuRI GGCC 3 cut(s) 18, 384, 579
BtsCI GGATG 1 cut(s) 776
BveI ACCTGC 1 cut(s) 603
Cac8I GCNNGC 2 cut(s) 452, 485
CfoI GCGC 2 cut(s) 238, 587
Cfr13I GGNCC 2 cut(s) 382, 577
DdeI CTNAG 9 cut(s) 81, 159, 327, 438, 546, 600, 637, 684, 722
DpnI GATC 2 cut(s) 624, 759
DpnII GATC 2 cut(s) 622, 757
EcoRII CCWGG 1 cut(s) 384
FaiI YATR 5 cut(s) 30, 40, 165, 167, 671
FaqI GGGAC 4 cut(s) 289, 744, 750, 756
FauI CCCGC 2 cut(s) 266, 720
FauNDI CATATG 1 cut(s) 165
FbaI TGATCA 2 cut(s) 622, 757
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 3 cut(s) 476, 629, 764
FokI GGATG 1 cut(s) 783
Fsp4HI GCNGC 3 cut(s) 476, 629, 764
GlaI GCGC 2 cut(s) 237, 586
GluI GCNGC 3 cut(s) 476, 629, 764
GsaI CCCAGC 2 cut(s) 118, 255
HaeIII GGCC 3 cut(s) 18, 384, 579
HapII CCGG 2 cut(s) 293, 512
HhaI GCGC 2 cut(s) 238, 587
Hin6I GCGC 2 cut(s) 236, 585
HinP1I GCGC 2 cut(s) 236, 585
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 2 cut(s) 104, 155
HpaII CCGG 2 cut(s) 293, 512
HphI GGTGA 2 cut(s) 371, 386
Hpy166II GTNNAC 3 cut(s) 40, 70, 706
Hpy188I TCNGA 6 cut(s) 14, 82, 439, 549, 622, 687
Hpy188III TCNNGA 1 cut(s) 750
Hpy8I GTNNAC 3 cut(s) 40, 70, 706
HpyAV CCTTC 3 cut(s) 170, 383, 598
HpyCH4III ACNGT 2 cut(s) 499, 747
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 3 cut(s) 169, 407, 644
HpyF10VI GCNNNNNNNGC 4 cut(s) 418, 451, 484, 489
HpyF3I CTNAG 9 cut(s) 81, 159, 327, 438, 546, 600, 637, 684, 722
HpySE526I ACGT 1 cut(s) 564
HspAI GCGC 2 cut(s) 236, 585
Ksp22I TGATCA 2 cut(s) 622, 757
Kzo9I GATC 2 cut(s) 622, 757
LmnI GCTCC 4 cut(s) 118, 233, 296, 515
Lsp1109I GCAGC 3 cut(s) 487, 640, 775
MaeII ACGT 1 cut(s) 564
MalI GATC 2 cut(s) 624, 759
MboI GATC 2 cut(s) 622, 757
MluCI AATT 3 cut(s) 23, 48, 218
MmeI TCCRAC 1 cut(s) 655
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 2 cut(s) 293, 512
MspR9I CCNGG 3 cut(s) 294, 386, 513
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 4 cut(s) 418, 451, 484, 489
NciI CCSGG 2 cut(s) 294, 513
NdeI CATATG 1 cut(s) 165
NdeII GATC 2 cut(s) 622, 757
PfeI GAWTC 2 cut(s) 104, 155
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 114
PkrI GCNGC 3 cut(s) 477, 630, 765
Psp6I CCWGG 1 cut(s) 384
PspFI CCCAGC 2 cut(s) 114, 251
PspGI CCWGG 1 cut(s) 384
PspPI GGNCC 2 cut(s) 382, 577
PsyI GACNNNGTC 1 cut(s) 563
SatI GCNGC 3 cut(s) 476, 629, 764
Sau3AI GATC 2 cut(s) 622, 757
Sau96I GGNCC 2 cut(s) 382, 577
ScrFI CCNGG 3 cut(s) 294, 386, 513
Sse9I AATT 3 cut(s) 23, 48, 218
SsiI CCGC 4 cut(s) 213, 246, 273, 727
SspI AATATT 1 cut(s) 712
StyD4I CCNGG 3 cut(s) 292, 384, 511
TaaI ACNGT 2 cut(s) 499, 747
TaiI ACGT 1 cut(s) 567
TaqI TCGA 1 cut(s) 749
TasI AATT 3 cut(s) 23, 48, 218
TfiI GAWTC 2 cut(s) 104, 155
TseI GCWGC 3 cut(s) 475, 628, 763
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 148
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.