Rh2CG323300

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001392
Physical Location & Seq
Reverse (-)
41651786 .. 41653479
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG323300.1

Sequence Viewer

Length: 1491 bp
ATGTCTTCTGAGCAACTTAGGAGGGAGAATGTGACGCCCGAGAGGGAAGTTCACATTGAGAAAGACAAGGTGCCGAAGATCACCAGCCACTTCGAGTCAATCACCGTGCATGTGCAAGAAGCCGGCGACAAAGACACGCCTATGGACAGCAGTCACAGCACCAAAGACTCGCACGACGATAAAGAGCGGAAGGGAAACACCGTTGGGGACTTGGGGATGGGAAAGTCCAGAGAGACTCGTGAGATCGGGAGTCACAAGCTGGAGTCCCTGGCGGGACAAATGAAGGAGGCTGAGGAGAAGAGAGGGAGGGAGAGTGGTGCCCGCGGAGTAGGGAAGTTTGAGGTTCAAGAAAGTGATGAGGATCAGAAGAAGGGAGGTAGAGGAGAGGGTAGAGAGGAAATGGTTGTTGTGAGTGAAGGGAAGGAGGATTCAAGGGTGAGACAAGGTGGGAGTGAAATGGAAGGCAGAAACATGGACATAATGGGCAGAGAGAGTAAGAGTGGAGCTCAAGGTGTCGGAAAGCTCGAAGTTATGAGGGTTGATGAAGAGAAGGGCAGAGAGGGTCAAGGTAGACAGCAAATGAGTTCTAAAGGGAGGGAGGGCGAGGGTAGAGAAAGAGAAGGTGGGAGCACACAAATGATGGGCAAAGGAGAGGGTATGACTATGACACAAGAGAGAGGAATGGACACGGAGATGATGAGAAGCAAAGTGGAAAGTCGCGGCCGCGAGCAGAGACGAGGCACAGAAACGGAGAGCAATGTGAACAAGCAGCAGCAGCAACCACGACAACAACAACCATCTCTGGAAGAAGTTTCGGCATTTAGACAAACAGCACAGCAGAATTCCATGGAGGCAATAAGAGCCGCAGAAGAGCGATATCAGAAAGCTAAAGAAACTGCAAACCAGACACTCGCTACTACAACCGAGAAGACTAAAGAAACAGCCCAGAAGGCTAAAGAAACTGCAAGCCAGACACTAGGCCTTGCAGCCCAGAAAGCTAAAGAAACTGCCAGTACTGCTACCGAGAAGGCTAAAGAAATTTCAAGCCAGACACTTGGTACTGCAACTGAGAAAGGAGCACAAGTGAAAGACATAGCACTGGAAAAAGGCCAACAAGGATTATATGCAGCAAAAGATACTCTATCAAGTGCAGGGAAGAAAACGGTGGATAACACGGTGCCATTAGCAGAGAAAACTAAAGACATGGCCGTGTCTGCAGGTTGGACTGCGGCGCACTACACAACTGAGGCAGCAGTGGAGGGAACCAAAGCAGCTGCAAGAGTAGTTGCCGGAGCAGCAGGGTATGCTGGTCATAAGGCTGTGGATATTGTGTCCAAGCCTTTGACTGTTGCTAAAGATGCTGCTGTGGTGACTGGTGAGAAAGCTGAGGAGTTCACTGCTCGGAAGAAGGAAGAGGCGAAGAGAGAATATGAGGCCAAGAAAGCAGCTGAAGCAAATAGTCCCAAGGTAGATATGTCATGTCGCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

496

Amino Acids

54.01

Weight (kDa)

6.79

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1208
AccB1I GGYRCC 3 cut(s) 70, 317, 1177
AccBSI CCGCTC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 571
AccII CGCG 3 cut(s) 324, 720, 726
AciI CCGC 8 cut(s) 187, 272, 322, 324, 720, 724, 864, 1229
AclWI GGATC 1 cut(s) 369
AcoI YGGCCR 2 cut(s) 721, 1206
AcsI RAATTY 2 cut(s) 841, 1038
AcuI CTGAAG 1 cut(s) 1470
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 2 cut(s) 1015, 1060
AgsI TTSAA 3 cut(s) 347, 432, 1044
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 8 cut(s) 259, 506, 523, 887, 998, 1274, 1385, 1448
AluI AGCT 8 cut(s) 259, 506, 523, 887, 998, 1274, 1385, 1448
Alw21I GWGCWC 3 cut(s) 508, 632, 1081
Alw26I GTCTC 3 cut(s) 227, 433, 727
AlwI GGATC 1 cut(s) 369
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 5 cut(s) 721, 979, 1108, 1206, 1434
ApoI RAATTY 2 cut(s) 841, 1038
AspLEI GCGC 1 cut(s) 1234
AsuHPI GGTGA 5 cut(s) 73, 94, 448, 1381, 1388
AvaI CYCGRG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 322
BanI GGYRCC 3 cut(s) 70, 317, 1177
BanII GRGCYC 1 cut(s) 508
BauI CACGAG 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 3 cut(s) 508, 632, 1081
BbvCI CCTCAGC 2 cut(s) 291, 1386
BccI CCATC 3 cut(s) 211, 634, 805
BceAI ACGGC 1 cut(s) 1193
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 3 cut(s) 227, 433, 727
BfaI CTAG 1 cut(s) 977
BfmI CTRYAG 1 cut(s) 1215
BfuAI ACCTGC 1 cut(s) 1208
BglI GCCNNNNNGGC 1 cut(s) 950
BmcAI AGTACT 1 cut(s) 1015
Bme1390I CCNGG 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 38
BmiI GGNNCC 4 cut(s) 72, 319, 1179, 1264
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 1348
BoxI GACNNNNGTC 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 935
BplI GAGNNNNNCTC 2 cut(s) 490, 522
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 2 cut(s) 291, 1386
BpuEI CTTGAG 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 4 cut(s) 267, 322, 846, 1464
BsaXI ACNNNNNCTCC 4 cut(s) 298, 328, 1068, 1098
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 3 cut(s) 1011, 1104, 1378
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 4 cut(s) 267, 322, 846, 1464
BseGI GGATG 1 cut(s) 222
BseJI GATNNNNATC 1 cut(s) 360
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 4 cut(s) 282, 1059, 1236, 1377
BseNI ACTGG 3 cut(s) 1011, 1104, 1378
BseRI GAGGAG 3 cut(s) 308, 396, 1403
BseSI GKGCMC 1 cut(s) 322
BseX3I CGGCCG 1 cut(s) 721
BsgI GTGCAG 1 cut(s) 1170
Bsh1236I CGCG 3 cut(s) 324, 720, 726
Bsh1285I CGRYCG 1 cut(s) 724
BshFI GGCC 5 cut(s) 723, 981, 1110, 1208, 1436
BshNI GGYRCC 3 cut(s) 70, 317, 1177
BsiEI CGRYCG 1 cut(s) 724
BsiHKAI GWGCWC 3 cut(s) 508, 632, 1081
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 2 cut(s) 123, 1290
BslFI GGGAC 4 cut(s) 221, 250, 288, 1446
BsmAI GTCTC 3 cut(s) 227, 433, 727
BsmBI CGTCTC 1 cut(s) 727
BsmFI GGGAC 4 cut(s) 221, 250, 288, 1446
BsnI GGCC 5 cut(s) 723, 981, 1110, 1208, 1436
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 4 cut(s) 322, 508, 632, 1081
Bsp143I GATC 3 cut(s) 78, 243, 361
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 8 cut(s) 187, 272, 322, 324, 720, 724, 864, 1229
BspANI GGCC 5 cut(s) 723, 981, 1110, 1208, 1436
BspCNI CTCAG 4 cut(s) 283, 1060, 1237, 1378
BspFNI CGCG 3 cut(s) 324, 720, 726
BspLI GGNNCC 4 cut(s) 72, 319, 1179, 1264
BspMAI CTGCAG 1 cut(s) 1219
BspMI ACCTGC 1 cut(s) 1208
BspPI GGATC 1 cut(s) 369
BspQI GCTCTTC 1 cut(s) 864
BspT107I GGYRCC 3 cut(s) 70, 317, 1177
BsrBI CCGCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 763
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 3 cut(s) 1011, 1104, 1378
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 267, 322, 846, 1464
BssMI GATC 3 cut(s) 78, 243, 361
BssNI GRCGYC 1 cut(s) 35
BssSI CACGAG 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 846, 1464
Bst2BI CACGAG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 5 cut(s) 106, 202, 1165, 1177, 1348
Bst6I CTCTTC 5 cut(s) 293, 540, 864, 1407, 1415
BstACI GRCGYC 1 cut(s) 35
BstAPI GCANNNNNTGC 1 cut(s) 1304
BstC8I GCNNGC 4 cut(s) 124, 322, 728, 967
BstDEI CTNAG 6 cut(s) 9, 17, 291, 1068, 1245, 1386
BstDSI CCRYGG 2 cut(s) 322, 846
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 3 cut(s) 324, 720, 726
BstHHI GCGC 1 cut(s) 1234
BstKTI GATC 3 cut(s) 81, 246, 364
BstMAI GTCTC 3 cut(s) 227, 433, 727
BstMBI GATC 3 cut(s) 78, 243, 361
BstMCI CGRYCG 1 cut(s) 724
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 113
BstPAI GACNNNNGTC 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 1215
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 3 cut(s) 324, 720, 726
BstV2I GAAGAC 1 cut(s) 935
BstXI CCANNNNNNTGG 1 cut(s) 1055
BstZI CGGCCG 1 cut(s) 721
BsuRI GGCC 5 cut(s) 723, 981, 1110, 1208, 1436
BtgI CCRYGG 2 cut(s) 322, 846
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 2 cut(s) 1260, 1395
BtsIMutI CAGTG 3 cut(s) 1097, 1260, 1395
BveI ACCTGC 1 cut(s) 1208
Cac8I GCNNGC 4 cut(s) 124, 322, 728, 967
CciNI GCGGCCGC 1 cut(s) 721
CfoI GCGC 1 cut(s) 1234
Cfr10I RCCGGY 1 cut(s) 122
Cfr42I CCGCGG 1 cut(s) 325
CseI GACGC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 1014, 1059
CviAII CATG 5 cut(s) 110, 472, 847, 1204, 1479
CviQI GTAC 2 cut(s) 1014, 1059
DdeI CTNAG 6 cut(s) 9, 17, 291, 1068, 1245, 1386
DpnI GATC 3 cut(s) 80, 245, 363
DpnII GATC 3 cut(s) 78, 243, 361
EaeI YGGCCR 2 cut(s) 721, 1206
EagI CGGCCG 1 cut(s) 721
Eam1104I CTCTTC 5 cut(s) 293, 540, 864, 1407, 1415
EarI CTCTTC 5 cut(s) 293, 540, 864, 1407, 1415
Ecl136II GAGCTC 1 cut(s) 506
EclXI CGGCCG 1 cut(s) 721
Eco130I CCWWGG 2 cut(s) 846, 1464
Eco147I AGGCCT 1 cut(s) 981
Eco24I GRGCYC 1 cut(s) 508
Eco32I GATATC 1 cut(s) 878
Eco52I CGGCCG 1 cut(s) 721
Eco53kI GAGCTC 1 cut(s) 506
Eco57I CTGAAG 1 cut(s) 1470
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 506
EcoRI GAATTC 1 cut(s) 841
EcoRII CCWGG 1 cut(s) 267
EcoRV GATATC 1 cut(s) 878
EcoT14I CCWWGG 2 cut(s) 846, 1464
EcoT38I GRGCYC 1 cut(s) 508
ErhI CCWWGG 2 cut(s) 846, 1464
Esp3I CGTCTC 1 cut(s) 727
FaeI CATG 5 cut(s) 113, 475, 850, 1207, 1482
FaqI GGGAC 4 cut(s) 221, 250, 288, 1446
FatI CATG 5 cut(s) 109, 471, 846, 1203, 1478
FauI CCCGC 2 cut(s) 265, 329
FblI GTMKAC 1 cut(s) 571
FokI GGATG 1 cut(s) 229
FriOI GRGCYC 1 cut(s) 508
FspBI CTAG 1 cut(s) 977
GlaI GCGC 1 cut(s) 1233
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 5 cut(s) 723, 981, 1110, 1208, 1436
HapII CCGG 2 cut(s) 123, 1290
HgaI GACGC 1 cut(s) 43
HhaI GCGC 1 cut(s) 1234
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 5 cut(s) 113, 475, 850, 1207, 1482
Hin6I GCGC 1 cut(s) 1232
HinP1I GCGC 1 cut(s) 1232
HinfI GANTC 6 cut(s) 95, 167, 235, 250, 263, 428
HpaII CCGG 2 cut(s) 123, 1290
HphI GGTGA 5 cut(s) 73, 94, 448, 1381, 1388
Hpy166II GTNNAC 4 cut(s) 52, 572, 763, 1395
Hpy188I TCNGA 5 cut(s) 10, 366, 518, 882, 1404
Hpy188III TCNNGA 5 cut(s) 228, 239, 247, 347, 803
Hpy8I GTNNAC 4 cut(s) 52, 572, 763, 1395
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 5 cut(s) 106, 202, 1165, 1177, 1348
HpyF3I CTNAG 6 cut(s) 9, 17, 291, 1068, 1245, 1386
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 5 cut(s) 113, 475, 850, 1207, 1482
HspAI GCGC 1 cut(s) 1232
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
KspI CCGCGG 1 cut(s) 325
Kzo9I GATC 3 cut(s) 78, 243, 361
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 4 cut(s) 503, 627, 1076, 1292
LweI GCATC 1 cut(s) 1348
MaeI CTAG 1 cut(s) 977
MaeIII GTNAC 4 cut(s) 31, 152, 251, 1369
MalI GATC 3 cut(s) 80, 245, 363
MbiI CCGCTC 1 cut(s) 187
MboI GATC 3 cut(s) 78, 243, 361
MhlI GDGCHC 4 cut(s) 322, 508, 632, 1081
MluCI AATT 2 cut(s) 841, 1038
MlyI GAGTC 5 cut(s) 104, 161, 229, 259, 272
MmeI TCCRAC 2 cut(s) 496, 1202
MroNI GCCGGC 1 cut(s) 122
MslI CAYNNNNRTG 4 cut(s) 140, 635, 692, 1208
MspA1I CMGCKG 3 cut(s) 324, 1274, 1448
MspI CCGG 2 cut(s) 123, 1290
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 3 cut(s) 324, 720, 726
NaeI GCCGGC 1 cut(s) 124
NcoI CCATGG 1 cut(s) 846
NdeII GATC 3 cut(s) 78, 243, 361
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 5 cut(s) 113, 475, 850, 1207, 1482
NlaIV GGNNCC 4 cut(s) 72, 319, 1179, 1264
NmuCI GTSAC 4 cut(s) 31, 152, 251, 1369
NotI GCGGCCGC 1 cut(s) 721
NspI RCATGY 1 cut(s) 113
PceI AGGCCT 1 cut(s) 981
PciSI GCTCTTC 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 428
PleI GAGTC 5 cut(s) 103, 161, 229, 258, 271
PpsI GAGTC 5 cut(s) 103, 161, 229, 258, 271
PshAI GACNNNNGTC 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 4 cut(s) 72, 319, 1179, 1264
PstI CTGCAG 1 cut(s) 1219
PvuII CAGCTG 2 cut(s) 1274, 1448
RsaI GTAC 2 cut(s) 1015, 1060
RsaNI GTAC 2 cut(s) 1014, 1059
RseI CAYNNNNRTG 4 cut(s) 140, 635, 692, 1208
SacI GAGCTC 1 cut(s) 508
SacII CCGCGG 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 864
Sau3AI GATC 3 cut(s) 78, 243, 361
ScaI AGTACT 1 cut(s) 1015
SchI GAGTC 5 cut(s) 104, 161, 229, 259, 272
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 4 cut(s) 322, 508, 632, 1081
SfaNI GCATC 1 cut(s) 1348
SfcI CTRYAG 1 cut(s) 1215
Sfr303I CCGCGG 1 cut(s) 325
SgrBI CCGCGG 1 cut(s) 325
SmiMI CAYNNNNRTG 4 cut(s) 140, 635, 692, 1208
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
Sse9I AATT 2 cut(s) 841, 1038
SseBI AGGCCT 1 cut(s) 981
SsiI CCGC 8 cut(s) 187, 272, 322, 324, 720, 724, 864, 1229
SspMI CTAG 1 cut(s) 977
SstI GAGCTC 1 cut(s) 508
StuI AGGCCT 1 cut(s) 981
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 846, 1464
TaaI ACNGT 5 cut(s) 106, 202, 1165, 1177, 1348
TaqI TCGA 2 cut(s) 93, 525
TasI AATT 2 cut(s) 841, 1038
TatI WGTACW 1 cut(s) 1013
TauI GCSGC 4 cut(s) 723, 726, 866, 1232
TfiI GAWTC 1 cut(s) 428
TscAI CASTG 3 cut(s) 1104, 1260, 1402
TseFI GTSAC 4 cut(s) 31, 152, 251, 1369
Tsp45I GTSAC 4 cut(s) 31, 152, 251, 1369
TspDTI ATGAA 2 cut(s) 296, 558
TspGWI ACGGA 2 cut(s) 704, 764
TspRI CASTG 3 cut(s) 1104, 1260, 1402
XapI RAATTY 2 cut(s) 841, 1038
XceI RCATGY 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 571
XspI CTAG 1 cut(s) 977
ZrmI AGTACT 1 cut(s) 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.