Rh2AG200900

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001392
Physical Location & Seq
Reverse (-)
20291851 .. 20292502
652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG200900.1

Sequence Viewer

Length: 384 bp
ATGCCGGTCGTAAGGCTGTGGATATTGTGTCCAAGCCTTTTGAGTGCTGCTAAAGATGCTGCTGTGGTGACTGGTGAGAAAGCTGAGGAGTTCACTGCTCGGAAGAAGGAAGAGGCGAAGAGAGAATATGAGGCCAAGAAAGCAGCTGAAGCAAATAGGACCAAGGGTTCTGAGACTACTTATCAAGGAGGAGAATCCAAATGCTGGGAGCAAGAGGAGACATGGACGAAACCAGTTCAGAGGGAATCTCAGCATATGCGGAGTGGCAGACCAACTGAAAAAACTAGCCAGAAACCGCTCAATTACAGAGGGAGCGCGAGAGGGCGGTACCTGGGTGCTGGGAACAATCGGCAGGACTTGAGGTGTGATACCGGGAGCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

14.28

Weight (kDa)

9.25

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 327
AccB7I CCANNNNNTGG 1 cut(s) 204
AccBSI CCGCTC 1 cut(s) 298
AccII CGCG 1 cut(s) 317
AciI CCGC 3 cut(s) 259, 296, 325
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 204
AjnI CCWGG 1 cut(s) 330
AloI GAACNNNNNNTCC 2 cut(s) 151, 183
AluBI AGCT 3 cut(s) 83, 146, 378
AluI AGCT 3 cut(s) 83, 146, 378
Alw26I GTCTC 2 cut(s) 167, 212
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 47, 59, 143
Asp718I GGTACC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 79, 86
AvaII GGWCC 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BanI GGYRCC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 3 cut(s) 34, 46, 155
BciT130I CCWGG 1 cut(s) 332
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 167, 212
BfaI CTAG 1 cut(s) 285
BisI GCNGC 3 cut(s) 48, 60, 144
BlsI GCNGC 3 cut(s) 49, 61, 145
Bme1390I CCNGG 2 cut(s) 332, 373
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 329
BmrFI CCNGG 2 cut(s) 332, 373
BmsI GCATC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 232, 264
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 379
BpuMI CCSGG 1 cut(s) 373
BsaJI CCNNGG 2 cut(s) 162, 331
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 76, 233
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 162, 331
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMII CTCAG 3 cut(s) 75, 162, 263
BseNI ACTGG 2 cut(s) 76, 233
BseRI GAGGAG 3 cut(s) 101, 204, 230
BseXI GCAGC 3 cut(s) 34, 46, 155
BseYI CCCAGC 2 cut(s) 204, 338
Bsh1236I CGCG 1 cut(s) 317
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 134
BshNI GGYRCC 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 5, 372
BslI CCNNNNNNNGG 1 cut(s) 204
BsmAI GTCTC 2 cut(s) 167, 212
BsnI GGCC 1 cut(s) 134
BspACI CCGC 3 cut(s) 259, 296, 325
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 3 cut(s) 76, 163, 262
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 327
BsrBI CCGCTC 1 cut(s) 298
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 76, 233
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 162, 331
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 332
Bst6I CTCTTC 2 cut(s) 105, 113
BstDEI CTNAG 3 cut(s) 84, 171, 249
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 1 cut(s) 317
BstMAI GTCTC 2 cut(s) 167, 212
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 56, 140, 149
BstNI CCWGG 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 330, 371
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 34, 46, 155
BsuRI GGCC 1 cut(s) 134
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 328
CviAII CATG 1 cut(s) 222
CviJI RGCY 7 cut(s) 16, 36, 83, 134, 146, 288, 378
CviKI_1 RGCY 7 cut(s) 16, 36, 83, 134, 146, 288, 378
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 3 cut(s) 84, 171, 249
Eam1104I CTCTTC 2 cut(s) 105, 113
EarI CTCTTC 2 cut(s) 105, 113
Eco130I CCWWGG 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 1 cut(s) 225
FaiI YATR 4 cut(s) 129, 223, 255, 257
FatI CATG 1 cut(s) 221
FauNDI CATATG 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 48, 60, 144
Fsp4HI GCNGC 3 cut(s) 48, 60, 144
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 316
GluI GCNGC 3 cut(s) 48, 60, 144
GsaI CCCAGC 2 cut(s) 208, 342
HaeIII GGCC 1 cut(s) 134
HapII CCGG 2 cut(s) 5, 372
HhaI GCGC 1 cut(s) 317
Hin1II CATG 1 cut(s) 225
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HinfI GANTC 2 cut(s) 194, 245
HpaII CCGG 2 cut(s) 5, 372
HphI GGTGA 2 cut(s) 79, 86
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 3 cut(s) 102, 172, 240
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 140, 149
HpyF3I CTNAG 3 cut(s) 84, 171, 249
Hsp92II CATG 1 cut(s) 225
HspAI GCGC 1 cut(s) 315
KpnI GGTACC 1 cut(s) 331
LmnI GCTCC 3 cut(s) 208, 312, 375
LpnPI CCDG 9 cut(s) 18, 57, 190, 246, 302, 317, 324, 338, 344
Lsp1109I GCAGC 3 cut(s) 34, 46, 155
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 67
MbiI CCGCTC 1 cut(s) 298
MboII GAAGA 3 cut(s) 115, 122, 130
MluCI AATT 1 cut(s) 301
MnlI CCTC 9 cut(s) 79, 106, 124, 182, 208, 234, 302, 314, 354
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 2 cut(s) 5, 372
MspR9I CCNGG 2 cut(s) 332, 373
MvaI CCWGG 1 cut(s) 332
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 3 cut(s) 56, 140, 149
NciI CCSGG 1 cut(s) 373
NdeI CATATG 1 cut(s) 255
NlaIII CATG 1 cut(s) 225
NlaIV GGNNCC 1 cut(s) 329
NmuCI GTSAC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 194, 245
PflMI CCANNNNNTGG 1 cut(s) 204
PkrI GCNGC 3 cut(s) 49, 61, 145
Psp6I CCWGG 1 cut(s) 330
PspFI CCCAGC 2 cut(s) 204, 338
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 329
PspPI GGNCC 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SatI GCNGC 3 cut(s) 48, 60, 144
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 2 cut(s) 332, 373
SetI ASST 5 cut(s) 85, 148, 333, 365, 380
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 1 cut(s) 301
SsiI CCGC 3 cut(s) 259, 296, 325
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 330, 371
StyI CCWWGG 1 cut(s) 162
TasI AATT 1 cut(s) 301
TfiI GAWTC 2 cut(s) 194, 245
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 3 cut(s) 47, 59, 143
Tsp45I GTSAC 1 cut(s) 67
TspRI CASTG 1 cut(s) 100
Van91I CCANNNNNTGG 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 159
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.