Rroxscaffold_7G00214810

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001392
Physical Location & Seq
Forward (+)
65448478 .. 65466636
18159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214810.1

Sequence Viewer

Length: 429 bp
ATGGCCATGTTTGCAGGTAAGACCACTCTGCATTATGTTGGTCATGTTGCGGTGAATTTGAAGGACAAGGCCACTGTGGTAGGTTGGACTGCAGCGCACTACGCAACTGAGGCAGCAGTGGAGGGAACCAAAGCAGCTGCAAGAGTAGTTGCCGGAGCAGCAGGGAGGAGAATCCAAATGCTGGGAGCAAGAGAGACATGGCCAAAACCAGTTCAGAGGGAATCTCATCATATGCAGAGTGGCAGACCAACTGAAAAAACCAGCCAGAAACTGCTCAGTTACAGAGGGAGTGCGAGAGGGCGGTACCTGGGTGCTGGGAACAATCAGCAGGACTTGAGGTTCAAACCTAGGACTGTGCGGCCCGAGGAACGAAGGTCTATGACCTTGGGCGAGAATATCGGGCAATCACGTCTTTGGCCGGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

15.71

Weight (kDa)

10.57

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 5
Acc65I GGTACC 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 181
AciI CCGC 3 cut(s) 50, 301, 358
AcoI YGGCCR 3 cut(s) 3, 200, 416
AcsI RAATTY 1 cut(s) 55
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 2 cut(s) 61, 343
AjiI CACGTC 1 cut(s) 410
AjnI CCWGG 1 cut(s) 306
AloI GAACNNNNNNTCC 2 cut(s) 323, 355
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 5 cut(s) 3, 69, 200, 359, 416
ApeKI GCWGC 5 cut(s) 92, 113, 134, 137, 158
ApoI RAATTY 1 cut(s) 55
Asp718I GGTACC 1 cut(s) 303
AspA2I CCTAGG 1 cut(s) 347
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 362
AvrII CCTAGG 1 cut(s) 347
BaeI ACNNNNGTAYC 2 cut(s) 295, 328
BalI TGGCCA 2 cut(s) 5, 202
BanI GGYRCC 1 cut(s) 303
BbvI GCAGC 5 cut(s) 104, 124, 125, 146, 170
BciT130I CCWGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 90
BfuAI ACCTGC 1 cut(s) 5
BisI GCNGC 6 cut(s) 93, 114, 135, 138, 159, 359
BlnI CCTAGG 1 cut(s) 347
BlsI GCNGC 6 cut(s) 94, 115, 136, 139, 160, 360
Bme1390I CCNGG 1 cut(s) 308
BmeT110I CYCGRG 1 cut(s) 362
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 360
BmiI GGNNCC 2 cut(s) 127, 305
BmrFI CCNGG 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 208, 240
BpuEI CTTGAG 1 cut(s) 355
BsaJI CCNNGG 4 cut(s) 307, 347, 363, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 4 cut(s) 307, 347, 363, 384
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 99, 289
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 5 cut(s) 104, 124, 125, 146, 170
BseYI CCCAGC 2 cut(s) 181, 314
BshFI GGCC 5 cut(s) 5, 71, 202, 361, 418
BshNI GGYRCC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 2 cut(s) 153, 419
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 188
BsnI GGCC 5 cut(s) 5, 71, 202, 361, 418
BsoBI CYCGRG 1 cut(s) 362
BspACI CCGC 3 cut(s) 50, 301, 358
BspANI GGCC 5 cut(s) 5, 71, 202, 361, 418
BspCNI CTCAG 2 cut(s) 100, 288
BspLI GGNNCC 2 cut(s) 127, 305
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 5
BspT107I GGYRCC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 4 cut(s) 307, 347, 363, 384
BssT1I CCWWGG 2 cut(s) 347, 384
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 76, 355
BstDEI CTNAG 2 cut(s) 108, 275
BstHHI GCGC 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 188
BstMWI GCNNNNNNNGC 4 cut(s) 11, 101, 110, 158
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 306
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 5 cut(s) 104, 124, 125, 146, 170
BsuRI GGCC 5 cut(s) 5, 71, 202, 361, 418
BtrI CACGTC 1 cut(s) 410
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 72, 123
BveI ACCTGC 1 cut(s) 5
CaiI CAGNNNCTG 1 cut(s) 271
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 360
Csp6I GTAC 1 cut(s) 304
CviAII CATG 3 cut(s) 7, 44, 198
CviJI RGCY 7 cut(s) 5, 71, 137, 202, 264, 361, 418
CviKI_1 RGCY 7 cut(s) 5, 71, 137, 202, 264, 361, 418
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 2 cut(s) 108, 275
EaeI YGGCCR 3 cut(s) 3, 200, 416
Eco130I CCWWGG 2 cut(s) 347, 384
Eco88I CYCGRG 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 306
EcoT14I CCWWGG 2 cut(s) 347, 384
ErhI CCWWGG 2 cut(s) 347, 384
FaeI CATG 3 cut(s) 10, 47, 201
FaiI YATR 7 cut(s) 8, 36, 45, 199, 231, 233, 380
FatI CATG 3 cut(s) 6, 43, 197
FauNDI CATATG 1 cut(s) 231
Fnu4HI GCNGC 6 cut(s) 93, 114, 135, 138, 159, 359
Fsp4HI GCNGC 6 cut(s) 93, 114, 135, 138, 159, 359
FspBI CTAG 1 cut(s) 348
GlaI GCGC 1 cut(s) 96
GluI GCNGC 6 cut(s) 93, 114, 135, 138, 159, 359
GsaI CCCAGC 2 cut(s) 185, 318
HaeIII GGCC 5 cut(s) 5, 71, 202, 361, 418
HapII CCGG 2 cut(s) 153, 419
HhaI GCGC 1 cut(s) 97
Hin1II CATG 3 cut(s) 10, 47, 201
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 2 cut(s) 171, 221
HpaII CCGG 2 cut(s) 153, 419
HphI GGTGA 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 216
HpyAV CCTTC 2 cut(s) 55, 366
HpyCH4III ACNGT 2 cut(s) 76, 355
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 5 cut(s) 14, 31, 92, 140, 235
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 101, 110, 158
HpyF3I CTNAG 2 cut(s) 108, 275
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 3 cut(s) 10, 47, 201
HspAI GCGC 1 cut(s) 95
KpnI GGTACC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 155, 185
Lsp1109I GCAGC 5 cut(s) 104, 124, 125, 146, 170
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 409
MaeIII GTNAC 1 cut(s) 278
MlsI TGGCCA 2 cut(s) 5, 202
MluCI AATT 1 cut(s) 55
MluNI TGGCCA 2 cut(s) 5, 202
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 8 cut(s) 103, 115, 159, 210, 278, 290, 330, 358
Mox20I TGGCCA 2 cut(s) 5, 202
MscI TGGCCA 2 cut(s) 5, 202
Msp20I TGGCCA 2 cut(s) 5, 202
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 2 cut(s) 153, 419
MspR9I CCNGG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 4 cut(s) 11, 101, 110, 158
NdeI CATATG 1 cut(s) 231
NlaIII CATG 3 cut(s) 10, 47, 201
NlaIV GGNNCC 2 cut(s) 127, 305
PfeI GAWTC 2 cut(s) 171, 221
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 6 cut(s) 94, 115, 136, 139, 160, 360
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 2 cut(s) 181, 314
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 127, 305
PspPI GGNCC 1 cut(s) 360
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
SatI GCNGC 6 cut(s) 93, 114, 135, 138, 159, 359
Sau96I GGNCC 1 cut(s) 360
ScrFI CCNGG 1 cut(s) 308
SetI ASST 9 cut(s) 19, 85, 139, 309, 341, 349, 377, 386, 412
SfcI CTRYAG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 334
SmoI CTYRAG 1 cut(s) 334
Sse9I AATT 1 cut(s) 55
SsiI CCGC 3 cut(s) 50, 301, 358
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 306
StyI CCWWGG 2 cut(s) 347, 384
TaaI ACNGT 2 cut(s) 76, 355
TaiI ACGT 1 cut(s) 412
TasI AATT 1 cut(s) 55
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 2 cut(s) 171, 221
TscAI CASTG 2 cut(s) 79, 123
TseI GCWGC 5 cut(s) 92, 113, 134, 137, 158
TspRI CASTG 2 cut(s) 79, 123
Van91I CCANNNNNTGG 1 cut(s) 181
XapI RAATTY 1 cut(s) 55
XmaJI CCTAGG 1 cut(s) 347
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.