Rorug02G0283800

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001392
Physical Location & Seq
Reverse (-)
30855920 .. 30859942
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0283800.1

Sequence Viewer

Length: 2610 bp
ATGGCGAACGCGACATCGGCCAACGGGACTAATATGACTAATGCATCAGATAGTTCAATAGTATGCTATGCCCCAACCATGATAACCACCAATGGTATTTGGCAGGGAGATAACCCTTTAGACTATTCCCTCCCTCTTTTCATCTTGCAGTTGACTCTGGTCGTCGTCACCACTCGCGTTCTCGTCCTCCTTCTCAAACCCTTTCGGCAACCTCGTGTCATCGCAGAAATCATGGGTGGAGTGTTATTGGGTCCATCGGTGCTTGGACAGTATAAACCATTCGCCGACACAATATTCCCCATGAGAAGTGTGATGGTGATCGAGACCATGGCAAATGTGGGTCTCCTCTACTTCCTCTTCTTGGTCGGCTTGGAGATGGACATTTCCGTTATTCGCCGCATGGGCAAAAGAGCCATTATCATTGCTGTTGCCGGCATGATCCTCCCCTTCGCCATTGGTGCTGCCTTTTCGTTCCTCATCCACGATAGAGGAAAATCAATGAACCAAGGTACCTTTATTCTCTTCCTCGGCGTTGCCCTCTCCGTCACCGCCTTCCCGGTCCTTGCTCGAGTCCTTGCCGAGCTCAAGCTTGTCAACACCGAGCTCGGCAGGATTGCTTTATCCTCGGCACTGGTAAACGACATGTTCGCTTGGGGTCTCTTAGCTCTGGCCATTGCCTTGGCGGAAAATGACTCCACTTCGCTGGCTTCCCTCTACGTTGTTTTGTCTAGCATTGCCTTTGTTTGCTTTTGCATATTCGTGGTCCGACCTGCAGTGTGCTGGATTATCCGAAGAACTCCAGAAGGAGAATCCTTTAGCGAGTTCTACATTTGCCTTATTCTCACCGGGGTCATGATCTCCGGGTTTGTTACTGACGCCATAGGAACACATTCCGTTTTTGGGGCTTTCGTGTTTGGTTTAGTCATCCCTAATGGACCACTTGGCGTCACTCTCATCGAGAAGCTCGAAGATTTTGTTTCGGGGCTTCTGCTCCCTCTTTTCTTTGCCATTAGCGGACTTAGAACCAATATTACTACGATCGAAGGAATTGGAACCTGGGGCCTTCTTGTACTTGTTATCTTTCTCGCTTGCATCGGAAAAATAGCCGGTACAGTCATTGTCTGCCTCTTTTACCAGATGCCATTTGACGAAGGGTTCACTCTAGGACTGCTCATGAACACAAAAGGCCTTGTCGAATTGATTGTCCTCAATGTCGGCAAAGACCAGAAGGTTCTAGACGATGAGGCCTTTGCAATTATGGTGATTGTAGCTGTTGTTATGACTGGAATCATTACACCAATAGTAACAACAGTTTACAAGCCGGCAAGAAGGTCTATACCTTACAAACGAAGAACGATTCAAAGGTCGAAGCCCGATGCGGAGTTACGAGTACTTGTTTGTGTCCACACACCTCGAAATGTGCCTACAATGATCAACCTTTTAGAAGCCTCTTACCCCACAAAGAGATCTCCTTTGTGCGTCTACATACTCCATTTAGTTGAGCTCAGTGGCCGTGCATCTGCAATGCTCATTGTTCATAACACTAGGAAATCCGGCAGGCCGGCGCTCAATCGAACCCAAGCTCAGTCTGACCACATCATAAATGCGTTCGAGAACTACGAGCAGCATGCCGGTTGTGTCTCGGTGCAGCCCTTGACTGCAATCTCACCATACTCTACAATGCACGAAGACATTTGCAACTTGGCAGAGGACAAACGTGTTGCCTTTTTAATTCTTCCTTTCCACAAACAGCAAACGGTGGATGGAGGAATGGAATCCATGAACCCGGCTTTTCGAACAATGAACCAAAATGTATTAGCAAATGCGCCATGCTCAGTCGGAATTCTAGTGGATAGAGGTTTCAATGGCTCCAATCGCGTGGCTGCAAACCAGGCATCTCACAATATAGTGGTACTTTTCTTCGGTGGACCGGACGACCGAGAGGCGTTGTCATATGCATGGAGGATGTCTGAACATCCAGGAATAACTCTTGCAGTCATGCGTTTTGTTCCCGGGCCGGACGTCGTGGAGCCGGTAGGAATAGTGAAACCCGATCCTGATGCCGCAAACAACGGCAACAACAATTCAAAGATATTGTGTGTCGAAACGGACGAGGGGTGGGAGAAGCAACTCGACGAGAAGTTGATAAATGAGTTTAGGACGCAAAATATGAACGACGAATCAATTGTGTACCTGGAGAAGGAGGTGAACAATGGAGAGGAGACGGTGGCGGTGGTAAGGTCAATGGACAGCGTGCATGACCTATTTATAGTCGGCAGAGGACAAGGCATAATATCGCCATTAACGGCGGGGCTAACTGATTGGAGTGAGTGCCCGGAACTCGGTGCTATTGGGGATTTGCTTGCGTCTTCCGACTTCGCAACAACCGCATCGGTTTTGGTAGTGCAACAATTCGTTGGTGATGAAGGGAATGCAGCAGTCGATCACGAGCTCGAGGATCCCAATGAGGAGCACGGAACACCGCAACCGGTAGCCAGCAGCCCAGCAGATGAACTTCAAAATGCGCAGTTTGGCAACATGCAGCGACATATGAACAAGCGGACTCAACCACCACCTAGGGTACAAAATAGTAACGTGTACAGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

869

Amino Acids

94.64

Weight (kDa)

5.34

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 59 - 436 1.5e-64 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 491 - 630 9e-31 Plant cation/H(+) antiporter domain
CHX17_C PF23259 635 - 674 2.4e-08 Plant cation/H(+) antiporter, C-terminal domain
CHX17_C PF23259 699 - 807 9e-10 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2025
Acc16I TGCGCA 1 cut(s) 2526
Acc36I ACCTGC 1 cut(s) 778
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AccI GTMKAC 1 cut(s) 1482
AccII CGCG 3 cut(s) 11, 177, 1878
AclWI GGATC 4 cut(s) 433, 2048, 2453, 2466
AcoI YGGCCR 3 cut(s) 18, 669, 1510
AcsI RAATTY 1 cut(s) 1842
AcyI GRCGYC 3 cut(s) 876, 945, 2022
AfaI GTAC 8 cut(s) 511, 1071, 1111, 1392, 1914, 2192, 2583, 2600
AfiI CCNNNNNNNGG 4 cut(s) 361, 900, 2200, 2342
AflIII ACRYGT 3 cut(s) 642, 1717, 2595
AgeI ACCGGT 1 cut(s) 2488
AgsI TTSAA 5 cut(s) 57, 1361, 1864, 2088, 2519
AjnI CCWGG 4 cut(s) 1055, 1890, 1978, 2193
AluBI AGCT 9 cut(s) 583, 589, 604, 665, 964, 1271, 1504, 1583, 2452
AluI AGCT 9 cut(s) 583, 589, 604, 665, 964, 1271, 1504, 1583, 2452
Alw21I GWGCWC 5 cut(s) 585, 606, 1506, 2454, 2475
Alw26I GTCTC 5 cut(s) 317, 347, 662, 1645, 2216
AlwI GGATC 4 cut(s) 433, 2048, 2453, 2466
Ama87I CYCGRG 3 cut(s) 567, 2012, 2453
AoxI GGCC 8 cut(s) 18, 669, 1060, 1186, 1245, 1510, 1559, 2015
ApeKI GCWGC 7 cut(s) 461, 1624, 1648, 1883, 2435, 2499, 2542
ApoI RAATTY 1 cut(s) 1842
ArsI GACNNNNNNTTYG 2 cut(s) 1188, 1220
AsiGI ACCGGT 1 cut(s) 2488
Asp700I GAANNNNTTC 1 cut(s) 889
Asp718I GGTACC 1 cut(s) 509
AspA2I CCTAGG 1 cut(s) 2576
AspLEI GCGC 3 cut(s) 1567, 1828, 2527
AspS9I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
AsuC2I CCSGG 7 cut(s) 557, 847, 862, 1787, 2013, 2014, 2336
AsuHPI GGTGA 8 cut(s) 160, 328, 538, 835, 1273, 1659, 2218, 2432
AsuII TTCGAA 1 cut(s) 1795
AvaI CYCGRG 3 cut(s) 567, 2012, 2453
AvaII GGWCC 5 cut(s) 251, 559, 763, 935, 1928
AvrII CCTAGG 1 cut(s) 2576
BaeGI GKGCMC 1 cut(s) 2336
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BalI TGGCCA 1 cut(s) 671
BamHI GGATCC 1 cut(s) 2458
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 4 cut(s) 585, 606, 1506, 2454
BauI CACGAG 2 cut(s) 213, 2447
BbsI GAAGAC 2 cut(s) 1695, 2361
Bbv12I GWGCWC 5 cut(s) 585, 606, 1506, 2454, 2475
BbvI GCAGC 7 cut(s) 448, 1636, 1660, 1870, 2447, 2511, 2554
BccI CCATC 4 cut(s) 262, 307, 370, 1757
BceAI ACGGC 3 cut(s) 1497, 2089, 2322
BcgI CGANNNNNNTGC 4 cut(s) 1610, 1644, 2042, 2076
BciT130I CCWGG 4 cut(s) 1057, 1892, 1980, 2195
BclI TGATCA 1 cut(s) 1431
BcnI CCSGG 7 cut(s) 557, 847, 862, 1787, 2013, 2014, 2336
BcoDI GTCTC 5 cut(s) 317, 347, 662, 1645, 2216
BfaI CTAG 6 cut(s) 729, 1163, 1235, 1545, 1847, 2577
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 1568
BfuAI ACCTGC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 402
BglII AGATCT 1 cut(s) 1466
BisI GCNGC 9 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2436, 2500, 2543
BlnI CCTAGG 1 cut(s) 2576
BlsI GCNGC 9 cut(s) 398, 463, 1626, 1650, 1885, 2065, 2437, 2501, 2544
BmcAI AGTACT 1 cut(s) 1392
Bme18I GGWCC 5 cut(s) 251, 559, 763, 935, 1928
BmeT110I CYCGRG 3 cut(s) 567, 2012, 2453
BmgT120I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
BmiI GGNNCC 7 cut(s) 252, 511, 1054, 1061, 1870, 2031, 2460
BmsI GCATC 8 cut(s) 53, 1101, 1128, 1366, 1526, 1904, 2050, 2399
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 2 cut(s) 1695, 2361
BpmI CTGGAG 2 cut(s) 783, 2216
Bpu14I TTCGAA 1 cut(s) 1795
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 7 cut(s) 557, 847, 862, 1787, 2013, 2014, 2336
BsaBI GATNNNNATC 1 cut(s) 317
BsaHI GRCGYC 3 cut(s) 876, 945, 2022
BsaI GGTCTC 3 cut(s) 317, 347, 662
BsaJI CCNNGG 9 cut(s) 327, 505, 526, 624, 678, 846, 1056, 2012, 2576
BsaWI WCCGGW 2 cut(s) 1930, 2488
Bsc4I CCNNNNNNNGG 4 cut(s) 361, 900, 2200, 2342
Bse118I RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
Bse1I ACTGG 2 cut(s) 636, 1288
Bse3DI GCAATG 4 cut(s) 420, 672, 732, 1530
Bse8I GATNNNNATC 1 cut(s) 317
BseBI CCWGG 4 cut(s) 1057, 1892, 1980, 2195
BseDI CCNNGG 9 cut(s) 327, 505, 526, 624, 678, 846, 1056, 2012, 2576
BseGI GGATG 5 cut(s) 477, 924, 1768, 1971, 1975
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 4 cut(s) 361, 900, 2200, 2342
BseMI GCAATG 4 cut(s) 420, 672, 732, 1530
BseMII CTCAG 3 cut(s) 1519, 1598, 1848
BseNI ACTGG 2 cut(s) 636, 1288
BseRI GAGGAG 3 cut(s) 335, 2234, 2483
BseSI GKGCMC 1 cut(s) 2336
BseXI GCAGC 7 cut(s) 448, 1636, 1660, 1870, 2447, 2511, 2554
BseYI CCCAGC 1 cut(s) 2503
BsgI GTGCAG 1 cut(s) 1667
Bsh1236I CGCG 3 cut(s) 11, 177, 1878
Bsh1285I CGRYCG 2 cut(s) 1041, 1939
BshFI GGCC 8 cut(s) 20, 671, 1062, 1188, 1247, 1512, 1561, 2017
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 2488
BsiEI CGRYCG 2 cut(s) 1041, 1939
BsiHKAI GWGCWC 5 cut(s) 585, 606, 1506, 2454, 2475
BsiHKCI CYCGRG 3 cut(s) 567, 2012, 2453
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 4 cut(s) 361, 900, 2200, 2342
BsmAI GTCTC 5 cut(s) 317, 347, 662, 1645, 2216
BsmBI CGTCTC 1 cut(s) 2216
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 2437
BsnI GGCC 8 cut(s) 20, 671, 1062, 1188, 1247, 1512, 1561, 2017
Bso31I GGTCTC 3 cut(s) 317, 347, 662
BsoBI CYCGRG 3 cut(s) 567, 2012, 2453
Bsp119I TTCGAA 1 cut(s) 1795
Bsp1286I GDGCHC 6 cut(s) 585, 606, 1506, 2336, 2454, 2475
Bsp1407I TGTACA 1 cut(s) 2598
Bsp143I GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
Bsp19I CCATGG 1 cut(s) 327
BspANI GGCC 8 cut(s) 20, 671, 1062, 1188, 1247, 1512, 1561, 2017
BspCNI CTCAG 3 cut(s) 1518, 1597, 1847
BspFNI CGCG 3 cut(s) 11, 177, 1878
BspHI TCATGA 2 cut(s) 852, 1173
BspLI GGNNCC 7 cut(s) 252, 511, 1054, 1061, 1870, 2031, 2460
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 4 cut(s) 433, 2048, 2453, 2466
BspT104I TTCGAA 1 cut(s) 1795
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 3 cut(s) 317, 347, 662
BsrDI GCAATG 4 cut(s) 420, 672, 732, 1530
BsrFI RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
BsrGI TGTACA 1 cut(s) 2598
BsrI ACTGG 2 cut(s) 636, 1288
BssAI RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
BssECI CCNNGG 9 cut(s) 327, 505, 526, 624, 678, 846, 1056, 2012, 2576
BssMI GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
BssNI GRCGYC 3 cut(s) 876, 945, 2022
BssSI CACGAG 2 cut(s) 213, 2447
BssT1I CCWWGG 4 cut(s) 327, 505, 678, 2576
Bst2BI CACGAG 2 cut(s) 213, 2447
Bst2UI CCWGG 4 cut(s) 1057, 1892, 1980, 2195
Bst4CI ACNGT 5 cut(s) 270, 1114, 1312, 1759, 2227
Bst6I CTCTTC 2 cut(s) 362, 527
BstACI GRCGYC 3 cut(s) 876, 945, 2022
BstAUI TGTACA 1 cut(s) 2598
BstBI TTCGAA 1 cut(s) 1795
BstDEI CTNAG 5 cut(s) 661, 1019, 1505, 1584, 1834
BstDSI CCRYGG 1 cut(s) 327
BstENI CCTNNNNNAGG 1 cut(s) 2198
BstF5I GGATG 5 cut(s) 477, 924, 1768, 1971, 1975
BstFNI CGCG 3 cut(s) 11, 177, 1878
BstH2I RGCGCY 1 cut(s) 1568
BstHHI GCGC 3 cut(s) 1567, 1828, 2527
BstKTI GATC 9 cut(s) 321, 441, 858, 1041, 1434, 1469, 2056, 2446, 2461
BstMAI GTCTC 5 cut(s) 317, 347, 662, 1645, 2216
BstMBI GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
BstMCI CGRYCG 2 cut(s) 1041, 1939
BstMWI GCNNNNNNNGC 6 cut(s) 17, 402, 458, 1875, 1892, 2387
BstNI CCWGG 4 cut(s) 1057, 1892, 1980, 2195
BstNSI RCATGY 3 cut(s) 646, 1631, 2542
BstPAI GACNNNNGTC 1 cut(s) 158
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 2336
BstUI CGCG 3 cut(s) 11, 177, 1878
BstV1I GCAGC 7 cut(s) 448, 1636, 1660, 1870, 2447, 2511, 2554
BstV2I GAAGAC 2 cut(s) 1695, 2361
BstX2I RGATCY 2 cut(s) 1466, 2458
BstXI CCANNNNNNTGG 3 cut(s) 679, 703, 1879
BstYI RGATCY 2 cut(s) 1466, 2458
BsuRI GGCC 8 cut(s) 20, 671, 1062, 1188, 1247, 1512, 1561, 2017
BtgI CCRYGG 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 5 cut(s) 477, 924, 1768, 1971, 1975
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 3 cut(s) 629, 780, 1513
BveI ACCTGC 1 cut(s) 778
CciI TCATGA 2 cut(s) 852, 1173
CfoI GCGC 3 cut(s) 1567, 1828, 2527
Cfr10I RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
Cfr13I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
Cfr9I CCCGGG 1 cut(s) 2012
CseI GACGC 5 cut(s) 884, 934, 1468, 2170, 2355
Csp6I GTAC 8 cut(s) 510, 1070, 1110, 1391, 1913, 2191, 2582, 2599
CspAI ACCGGT 1 cut(s) 2488
CviQI GTAC 8 cut(s) 510, 1070, 1110, 1391, 1913, 2191, 2582, 2599
DdeI CTNAG 5 cut(s) 661, 1019, 1505, 1584, 1834
DpnI GATC 9 cut(s) 320, 440, 857, 1040, 1433, 1468, 2055, 2445, 2460
DpnII GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
EaeI YGGCCR 3 cut(s) 18, 669, 1510
Eam1104I CTCTTC 2 cut(s) 362, 527
EarI CTCTTC 2 cut(s) 362, 527
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 4 cut(s) 583, 604, 1504, 2452
Eco130I CCWWGG 4 cut(s) 327, 505, 678, 2576
Eco147I AGGCCT 2 cut(s) 1188, 1247
Eco24I GRGCYC 4 cut(s) 585, 606, 1506, 2454
Eco31I GGTCTC 3 cut(s) 317, 347, 662
Eco47I GGWCC 5 cut(s) 251, 559, 763, 935, 1928
Eco53kI GAGCTC 4 cut(s) 583, 604, 1504, 2452
Eco88I CYCGRG 3 cut(s) 567, 2012, 2453
EcoICRI GAGCTC 4 cut(s) 583, 604, 1504, 2452
EcoNI CCTNNNNNAGG 1 cut(s) 2198
EcoO109I RGGNCCY 1 cut(s) 1060
EcoRI GAATTC 1 cut(s) 1842
EcoRII CCWGG 4 cut(s) 1055, 1890, 1978, 2193
EcoT14I CCWWGG 4 cut(s) 327, 505, 678, 2576
EcoT22I ATGCAT 2 cut(s) 46, 1960
EcoT38I GRGCYC 4 cut(s) 585, 606, 1506, 2454
ErhI CCWWGG 4 cut(s) 327, 505, 678, 2576
Esp3I CGTCTC 1 cut(s) 2216
FaqI GGGAC 1 cut(s) 40
FauI CCCGC 1 cut(s) 2302
FauNDI CATATG 2 cut(s) 1954, 2550
FbaI TGATCA 1 cut(s) 1431
FblI GTMKAC 1 cut(s) 1482
Fnu4HI GCNGC 9 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2436, 2500, 2543
FokI GGATG 5 cut(s) 464, 911, 1775, 1962, 1978
FriOI GRGCYC 4 cut(s) 585, 606, 1506, 2454
Fsp4HI GCNGC 9 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2436, 2500, 2543
FspBI CTAG 6 cut(s) 729, 1163, 1235, 1545, 1847, 2577
FspI TGCGCA 1 cut(s) 2526
GlaI GCGC 3 cut(s) 1566, 1827, 2526
GluI GCNGC 9 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2436, 2500, 2543
GsaI CCCAGC 1 cut(s) 2507
GsuI CTGGAG 2 cut(s) 783, 2216
HaeII RGCGCY 1 cut(s) 1568
HaeIII GGCC 8 cut(s) 20, 671, 1062, 1188, 1247, 1512, 1561, 2017
HgaI GACGC 5 cut(s) 884, 934, 1468, 2170, 2355
HhaI GCGC 3 cut(s) 1567, 1828, 2527
Hin1I GRCGYC 3 cut(s) 876, 945, 2022
Hin6I GCGC 3 cut(s) 1565, 1826, 2525
HinP1I GCGC 3 cut(s) 1565, 1826, 2525
HincII GTYRAC 2 cut(s) 153, 595
HindII GTYRAC 2 cut(s) 153, 595
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 9 cut(s) 154, 570, 692, 809, 1287, 1357, 1775, 2180, 2563
HphI GGTGA 8 cut(s) 160, 328, 538, 835, 1273, 1659, 2218, 2432
Hpy188I TCNGA 8 cut(s) 49, 767, 791, 1097, 1591, 1841, 1972, 2374
Hpy188III TCNNGA 9 cut(s) 322, 800, 853, 958, 1174, 1235, 1612, 2057, 2447
Hpy99I CGWCG 4 cut(s) 167, 2027, 2138, 2180
HpyCH4III ACNGT 5 cut(s) 270, 1114, 1312, 1759, 2227
HpyCH4IV ACGT 4 cut(s) 717, 1717, 2022, 2595
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 402, 458, 1875, 1892, 2387
HpyF3I CTNAG 5 cut(s) 661, 1019, 1505, 1584, 1834
HpySE526I ACGT 4 cut(s) 717, 1717, 2022, 2595
Hsp92I GRCGYC 3 cut(s) 876, 945, 2022
HspAI GCGC 3 cut(s) 1565, 1826, 2525
KpnI GGTACC 1 cut(s) 513
KroI GCCGGC 3 cut(s) 431, 1321, 1561
KroNI GCCGGC 3 cut(s) 433, 1323, 1563
Ksp22I TGATCA 1 cut(s) 1431
Kzo9I GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
LmnI GCTCC 4 cut(s) 996, 1874, 2029, 2470
Lsp1109I GCAGC 7 cut(s) 448, 1636, 1660, 1870, 2447, 2511, 2554
LweI GCATC 8 cut(s) 53, 1101, 1128, 1366, 1526, 1904, 2050, 2399
MaeI CTAG 6 cut(s) 729, 1163, 1235, 1545, 1847, 2577
MaeII ACGT 4 cut(s) 717, 1717, 2022, 2595
MaeIII GTNAC 7 cut(s) 166, 544, 868, 946, 1303, 1383, 2591
MalI GATC 9 cut(s) 320, 440, 857, 1040, 1433, 1468, 2055, 2445, 2460
MboI GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
MboII GAAGA 9 cut(s) 349, 514, 804, 980, 1362, 1700, 1727, 1912, 2361
MfeI CAATTG 1 cut(s) 2184
MflI RGATCY 2 cut(s) 1466, 2458
MhlI GDGCHC 6 cut(s) 585, 606, 1506, 2336, 2454, 2475
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 8 cut(s) 1047, 1196, 1254, 1731, 1842, 2083, 2184, 2411
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 4 cut(s) 148, 579, 686, 2557
MmeI TCCRAC 3 cut(s) 790, 1819, 2397
Mox20I TGGCCA 1 cut(s) 671
Mph1103I ATGCAT 2 cut(s) 46, 1960
MroNI GCCGGC 3 cut(s) 431, 1321, 1561
MroXI GAANNNNTTC 1 cut(s) 889
MscI TGGCCA 1 cut(s) 671
MseI TTAA 2 cut(s) 1730, 2303
MslI CAYNNNNRTG 2 cut(s) 758, 1957
Msp20I TGGCCA 1 cut(s) 671
MunI CAATTG 1 cut(s) 2184
Mva1269I GAATGC 1 cut(s) 2437
MvaI CCWGG 4 cut(s) 1057, 1892, 1980, 2195
MvnI CGCG 3 cut(s) 11, 177, 1878
MwoI GCNNNNNNNGC 6 cut(s) 17, 402, 458, 1875, 1892, 2387
NaeI GCCGGC 3 cut(s) 433, 1323, 1563
NciI CCSGG 7 cut(s) 557, 847, 862, 1787, 2013, 2014, 2336
NcoI CCATGG 1 cut(s) 327
NdeI CATATG 2 cut(s) 1954, 2550
NdeII GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
NgoMIV GCCGGC 3 cut(s) 431, 1321, 1561
NlaIV GGNNCC 7 cut(s) 252, 511, 1054, 1061, 1870, 2031, 2460
NmeAIII GCCGAG 4 cut(s) 507, 585, 604, 605
NmuCI GTSAC 3 cut(s) 166, 544, 946
NsbI TGCGCA 1 cut(s) 2526
NsiI ATGCAT 2 cut(s) 46, 1960
NspI RCATGY 3 cut(s) 646, 1631, 2542
NspV TTCGAA 1 cut(s) 1795
PaeI GCATGC 1 cut(s) 1631
PaeR7I CTCGAG 2 cut(s) 567, 2453
PagI TCATGA 2 cut(s) 852, 1173
PceI AGGCCT 2 cut(s) 1188, 1247
PciI ACATGT 1 cut(s) 642
PcsI WCGNNNNNNNCGW 4 cut(s) 211, 963, 1617, 2109
PctI GAATGC 1 cut(s) 2437
PdiI GCCGGC 3 cut(s) 433, 1323, 1563
PdmI GAANNNNTTC 1 cut(s) 889
PfeI GAWTC 5 cut(s) 809, 1287, 1357, 1775, 2180
PfoI TCCNGGA 1 cut(s) 1978
PinAI ACCGGT 1 cut(s) 2488
PkrI GCNGC 9 cut(s) 398, 463, 1626, 1650, 1885, 2065, 2437, 2501, 2544
Ple19I CGATCG 1 cut(s) 1041
PleI GAGTC 4 cut(s) 148, 578, 686, 2557
PpsI GAGTC 4 cut(s) 148, 578, 686, 2557
PscI ACATGT 1 cut(s) 642
PshAI GACNNNNGTC 1 cut(s) 158
Psp124BI GAGCTC 4 cut(s) 585, 606, 1506, 2454
Psp6I CCWGG 4 cut(s) 1055, 1890, 1978, 2193
PspFI CCCAGC 1 cut(s) 2503
PspGI CCWGG 4 cut(s) 1055, 1890, 1978, 2193
PspN4I GGNNCC 7 cut(s) 252, 511, 1054, 1061, 1870, 2031, 2460
PspPI GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
PspXI VCTCGAGB 2 cut(s) 567, 2453
PsrI GAACNNNNNNTAC 2 cut(s) 1015, 1047
PstI CTGCAG 1 cut(s) 775
PsuI RGATCY 2 cut(s) 1466, 2458
PvuI CGATCG 1 cut(s) 1041
RsaI GTAC 8 cut(s) 511, 1071, 1111, 1392, 1914, 2192, 2583, 2600
RsaNI GTAC 8 cut(s) 510, 1070, 1110, 1391, 1913, 2191, 2582, 2599
RseI CAYNNNNRTG 2 cut(s) 758, 1957
SacI GAGCTC 4 cut(s) 585, 606, 1506, 2454
SaqAI TTAA 2 cut(s) 1730, 2303
SatI GCNGC 9 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2436, 2500, 2543
Sau3AI GATC 9 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2443, 2458
Sau96I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 4 cut(s) 148, 579, 686, 2557
SduI GDGCHC 6 cut(s) 585, 606, 1506, 2336, 2454, 2475
SfaNI GCATC 8 cut(s) 53, 1101, 1128, 1366, 1526, 1904, 2050, 2399
SfcI CTRYAG 1 cut(s) 771
Sfr274I CTCGAG 2 cut(s) 567, 2453
SfuI TTCGAA 1 cut(s) 1795
SinI GGWCC 5 cut(s) 251, 559, 763, 935, 1928
SlaI CTCGAG 2 cut(s) 567, 2453
SmaI CCCGGG 1 cut(s) 2014
SmiMI CAYNNNNRTG 2 cut(s) 758, 1957
SmlI CTYRAG 3 cut(s) 567, 584, 2453
SmoI CTYRAG 3 cut(s) 567, 584, 2453
SphI GCATGC 1 cut(s) 1631
Sse9I AATT 8 cut(s) 1047, 1196, 1254, 1731, 1842, 2083, 2184, 2411
SseBI AGGCCT 2 cut(s) 1188, 1247
SspI AATATT 2 cut(s) 294, 1030
SspMI CTAG 6 cut(s) 729, 1163, 1235, 1545, 1847, 2577
SstI GAGCTC 4 cut(s) 585, 606, 1506, 2454
StuI AGGCCT 2 cut(s) 1188, 1247
StyI CCWWGG 4 cut(s) 327, 505, 678, 2576
TaaI ACNGT 5 cut(s) 270, 1114, 1312, 1759, 2227
TaiI ACGT 4 cut(s) 720, 1720, 2025, 2598
TaqII GACCGA 1 cut(s) 1953
TasI AATT 8 cut(s) 1047, 1196, 1254, 1731, 1842, 2083, 2184, 2411
TatI WGTACW 3 cut(s) 1069, 1390, 2598
TauI GCSGC 2 cut(s) 399, 2066
TfiI GAWTC 5 cut(s) 809, 1287, 1357, 1775, 2180
Tru1I TTAA 2 cut(s) 1730, 2303
Tru9I TTAA 2 cut(s) 1730, 2303
TscAI CASTG 3 cut(s) 636, 780, 1513
TseFI GTSAC 3 cut(s) 166, 544, 946
TseI GCWGC 7 cut(s) 461, 1624, 1648, 1883, 2435, 2499, 2542
Tsp45I GTSAC 3 cut(s) 166, 544, 946
TspGWI ACGGA 5 cut(s) 376, 532, 883, 2123, 2490
TspMI CCCGGG 1 cut(s) 2012
TspRI CASTG 3 cut(s) 636, 780, 1513
VpaK11BI GGWCC 5 cut(s) 251, 559, 763, 935, 1928
XagI CCTNNNNNAGG 1 cut(s) 2198
XapI RAATTY 1 cut(s) 1842
XbaI TCTAGA 1 cut(s) 1234
XceI RCATGY 3 cut(s) 646, 1631, 2542
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XhoI CTCGAG 2 cut(s) 567, 2453
XmaI CCCGGG 1 cut(s) 2012
XmaJI CCTAGG 1 cut(s) 2576
XmiI GTMKAC 1 cut(s) 1482
XmnI GAANNNNTTC 1 cut(s) 889
XspI CTAG 6 cut(s) 729, 1163, 1235, 1545, 1847, 2577
ZraI GACGTC 1 cut(s) 2023
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 2 cut(s) 46, 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.