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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 523 |
| AcsI | RAATTY | 2 cut(s) | 47, 542 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 224, 433, 511 |
| AjnI | CCWGG | 1 cut(s) | 211 |
| AluBI | AGCT | 1 cut(s) | 405 |
| AluI | AGCT | 1 cut(s) | 405 |
| AlwI | GGATC | 1 cut(s) | 523 |
| ApoI | RAATTY | 2 cut(s) | 47, 542 |
| AspS9I | GGNCC | 1 cut(s) | 215 |
| AvaII | GGWCC | 1 cut(s) | 215 |
| BciT130I | CCWGG | 1 cut(s) | 213 |
| BclI | TGATCA | 1 cut(s) | 507 |
| BfaI | CTAG | 1 cut(s) | 652 |
| BfmI | CTRYAG | 1 cut(s) | 19 |
| Bme1390I | CCNGG | 1 cut(s) | 213 |
| Bme18I | GGWCC | 1 cut(s) | 215 |
| BmgT120I | GGNCC | 1 cut(s) | 215 |
| BmrFI | CCNGG | 1 cut(s) | 213 |
| BpuEI | CTTGAG | 1 cut(s) | 416 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 224, 433, 511 |
| BseBI | CCWGG | 1 cut(s) | 213 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 224, 433, 511 |
| BseMII | CTCAG | 1 cut(s) | 510 |
| BseRI | GAGGAG | 1 cut(s) | 419 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 224, 433, 511 |
| Bsp143I | GATC | 2 cut(s) | 507, 515 |
| BspCNI | CTCAG | 1 cut(s) | 511 |
| BspPI | GGATC | 1 cut(s) | 523 |
| BssMI | GATC | 2 cut(s) | 507, 515 |
| Bst2UI | CCWGG | 1 cut(s) | 213 |
| Bst6I | CTCTTC | 1 cut(s) | 226 |
| BstDEI | CTNAG | 1 cut(s) | 519 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 222, 431 |
| BstKTI | GATC | 2 cut(s) | 510, 518 |
| BstMBI | GATC | 2 cut(s) | 507, 515 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 402, 411 |
| BstNI | CCWGG | 1 cut(s) | 213 |
| BstSCI | CCNGG | 1 cut(s) | 211 |
| BstSFI | CTRYAG | 1 cut(s) | 19 |
| BstX2I | RGATCY | 1 cut(s) | 515 |
| BstYI | RGATCY | 1 cut(s) | 515 |
| Cfr13I | GGNCC | 1 cut(s) | 215 |
| CsiI | ACCWGGT | 1 cut(s) | 211 |
| CviJI | RGCY | 3 cut(s) | 405, 575, 651 |
| CviKI_1 | RGCY | 3 cut(s) | 405, 575, 651 |
| DdeI | CTNAG | 1 cut(s) | 519 |
| DpnI | GATC | 2 cut(s) | 509, 517 |
| DpnII | GATC | 2 cut(s) | 507, 515 |
| Eam1104I | CTCTTC | 1 cut(s) | 226 |
| EarI | CTCTTC | 1 cut(s) | 226 |
| Eco47I | GGWCC | 1 cut(s) | 215 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 222, 431 |
| EcoO109I | RGGNCCY | 1 cut(s) | 215 |
| EcoRII | CCWGG | 1 cut(s) | 211 |
| FaiI | YATR | 5 cut(s) | 12, 42, 91, 105, 124 |
| FbaI | TGATCA | 1 cut(s) | 507 |
| FspBI | CTAG | 1 cut(s) | 652 |
| HinfI | GANTC | 2 cut(s) | 443, 589 |
| Hpy188I | TCNGA | 3 cut(s) | 64, 204, 520 |
| Hpy188III | TCNNGA | 1 cut(s) | 505 |
| HpyCH4V | TGCA | 2 cut(s) | 168, 414 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 402, 411 |
| HpyF3I | CTNAG | 1 cut(s) | 519 |
| Ksp22I | TGATCA | 1 cut(s) | 507 |
| Kzo9I | GATC | 2 cut(s) | 507, 515 |
| LpnPI | CCDG | 8 cut(s) | 178, 178, 198, 200, 225, 496, 518, 578 |
| MabI | ACCWGGT | 1 cut(s) | 211 |
| MaeI | CTAG | 1 cut(s) | 652 |
| MaeIII | GTNAC | 1 cut(s) | 182 |
| MalI | GATC | 2 cut(s) | 509, 517 |
| MboI | GATC | 2 cut(s) | 507, 515 |
| MboII | GAAGA | 5 cut(s) | 17, 106, 243, 463, 483 |
| MflI | RGATCY | 1 cut(s) | 515 |
| MluCI | AATT | 7 cut(s) | 33, 47, 66, 129, 383, 438, 542 |
| MmeI | TCCRAC | 1 cut(s) | 602 |
| MnlI | CCTC | 5 cut(s) | 23, 228, 437, 440, 476 |
| MseI | TTAA | 4 cut(s) | 36, 179, 534, 633 |
| MspR9I | CCNGG | 1 cut(s) | 213 |
| MvaI | CCWGG | 1 cut(s) | 213 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 402, 411 |
| NdeII | GATC | 2 cut(s) | 507, 515 |
| NmuCI | GTSAC | 1 cut(s) | 182 |
| PfeI | GAWTC | 2 cut(s) | 443, 589 |
| PpuMI | RGGWCCY | 1 cut(s) | 215 |
| Psp5II | RGGWCCY | 1 cut(s) | 215 |
| Psp6I | CCWGG | 1 cut(s) | 211 |
| PspGI | CCWGG | 1 cut(s) | 211 |
| PspPI | GGNCC | 1 cut(s) | 215 |
| PspPPI | RGGWCCY | 1 cut(s) | 215 |
| PsuI | RGATCY | 1 cut(s) | 515 |
| SaqAI | TTAA | 4 cut(s) | 36, 179, 534, 633 |
| Sau3AI | GATC | 2 cut(s) | 507, 515 |
| Sau96I | GGNCC | 1 cut(s) | 215 |
| ScrFI | CCNGG | 1 cut(s) | 213 |
| SetI | ASST | 7 cut(s) | 137, 189, 217, 407, 429, 438, 487 |
| SexAI | ACCWGGT | 1 cut(s) | 211 |
| SfcI | CTRYAG | 1 cut(s) | 19 |
| SinI | GGWCC | 1 cut(s) | 215 |
| SmlI | CTYRAG | 1 cut(s) | 431 |
| SmoI | CTYRAG | 1 cut(s) | 431 |
| Sse9I | AATT | 7 cut(s) | 33, 47, 66, 129, 383, 438, 542 |
| SspMI | CTAG | 1 cut(s) | 652 |
| StyD4I | CCNGG | 1 cut(s) | 211 |
| TaqI | TCGA | 2 cut(s) | 75, 579 |
| TasI | AATT | 7 cut(s) | 33, 47, 66, 129, 383, 438, 542 |
| TfiI | GAWTC | 2 cut(s) | 443, 589 |
| Tru1I | TTAA | 4 cut(s) | 36, 179, 534, 633 |
| Tru9I | TTAA | 4 cut(s) | 36, 179, 534, 633 |
| TseFI | GTSAC | 1 cut(s) | 182 |
| Tsp45I | GTSAC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 2 cut(s) | 434, 602 |
| TspGWI | ACGGA | 1 cut(s) | 379 |
| VpaK11BI | GGWCC | 1 cut(s) | 215 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 222, 431 |
| XapI | RAATTY | 2 cut(s) | 47, 542 |
| XspI | CTAG | 1 cut(s) | 652 |