Prupe.3G123300_v2.0.a1

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001392
Physical Location & Seq
Forward (+)
11015627 .. 11018296
2670 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G123300.1

Sequence Viewer

Length: 2085 bp
ATGGCTTCTGAGCAGCAAATGAGAAGGGAAAATACCACCTCGGAGAGGGAGGTGGAGATTGAGAAAGATAAGGTGCCGAAGATCACGACCCACTTTGAGGCTCTGAGAGGGGCGGAAGGCAAAGACACTTCTCTGGACAGCAGCAAGCAGTCGCAGCAGCAGCATGAGGATAAAGCGCGGAGTGGAAGCACGGCAGGTGATGTGGGCATGGGAAAAGCAGAAGAGGCTCATGAGCTCGAGTCTCTCAAGGATCAAGTGGAGAAGGACAAGGATAAGGAGAGAGAAGCGCAGGCTGATCAAGCGAGGACTGCGAAAATGGTGGCGGATAAAGAGGCCGAGGCTAAGAGAGAGAGCGAAGGCCGCACTCAAGGTCAAGGAGAAGGCCAAGGTGGGAAATTTGAAGAGCACCACCAAGGGCAAGGTCAAAGTGAAGGTCAAGGCCAAGGTGGAGCGCGTCCTCAAACTCATACGAAGGTTGAAGAGCACCAACGACAAGCAGGAGCTCAAGGCCAAGGCGGTGGTCAGGTTGGAGAGCACCAACGACAAGTCGGGGCTCAAGGACAAGAAGGTGGTGGTCAATATCATGCGAAGGTTGAAGAGCACCAAGGACAAGGGGGAGGTCGTCAATCTCTTGGGCAAGCTGGAGGTGAAGGAAAAGGCAGAGCTCAATCTGTTGGTAAGTTTGAAGTGAGTGGTGAGGAAGAGAAGGGTTCGGCCAATAAAGGCCGAGAAAGTACTGAAGTTGATAAACAATCTAGATGCACACAGATAGGCCAGGGTATAGAGAAAATGACCCTCGAGGAAGAGAAGAGTTCTAAAGGAGGCAGAGAAAATGAGATGAAAAGCAGGGAAGAAAAGGAAGGAAGAAGCACAAACACGACGGGCAGAGGAGATCAGGGTAGAGAGGAAATGGGTGGTGGGGAAGAGAGAGGCTCTATAGGCAGAGAAGAAAAGGAGGGTCGTCAGCAGCAAAACAAAGAACAGCAACCATCTTTGGAAGAAATCTCAAAGTTTAGAGCAGCAGCACAGCAGAATTCCATGGAGGCAATAAGAGCAGCAGAGGAGCGTTATGCCCAGGCTAAAGAATCGGCGAGGCAAGGGATTGGCAGCGCAACCGAGTTTGTCTCAGAGAAAGGTGAGCAAACCAAGGAGACACTCGTCCAAGGAGCACAAACGGCAAAAGAAACTCTTAGCAGCGCAGGCAAGACGGTCGTGGAGAAGACAGCCCCAGTAGCGGAGAACGCGAAAGATGTGGCGGTGTCCGCGGGACAGACCACTCTGCATTACGTGGGAGAAGGGGCTGCGAAGGCCAAGGACGTGACGTTGGAGGGCGGGAAGACTGCGGGGGCATATGCCGGTGACGTGGCTGTGGGTTTGAAGGACAAGGCGACTGTGGCTGGCTGGAGTGCCGCGCATTACACGACAGAGGCGGCAGTGGAGGGGACCAAGTTGGCGGCTAGAACTGTGAAAGGGGCGGCGGAGTATGCGGGTCATACAGCTGTGGATATTGTTTCCAAGCCCTTGATTATGGCTAAAGATGCTGCGGCGGTGGCAGGTGAGAAGGCAGAGGAGTACACTGCTAGGAAGAGAGAGGAGGCACAGAGAGAAGTGGAGGCCAAGAGGTCAGCTGAGCAAAATAAGTCACAGGGGGGTCTGACTCAACAAGGAAGAGAAAAAGAGGGATCCAAAGGGGACCAAGGGGAGACTGGTCAGGAATGGCAGAGTCAAGTTCAGGGCACCGTGAGAGGTGAGGACATCAGTCGGGATCAGAGAAGAGAGGAGGGTCAAAAGGGAACGCAGAGCGAAGGTGGTGATCAGCAAAGAAGAGAGGAGAGGACTTATGAGGAAGGCGGTGACAAAGCGAGTACTGAGGAATACAGGAGTGGCATGGATACCTCTCAAGTTGCCGGTGGCGGTGCCGGCGTAGGGGTGGTGCTGGGAGCAATCGGAGAGACTTTGGTTGAGATCGCCCAGACGACAAAAGACATGGTGATCGGGCAAGAGGAGGAGCAGAATGGTAACCAGGGGGAGTGGAATTCATCAACTGATCAACAGGGCAAGCAAGGGGTGGGCAAGATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

695

Amino Acids

74.12

Weight (kDa)

5.26

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 185, 1544
AbsI CCTCGAGG 1 cut(s) 797
Acc36I ACCTGC 2 cut(s) 185, 1544
AccB1I GGYRCC 3 cut(s) 73, 1736, 1916
AccII CGCG 5 cut(s) 178, 454, 1244, 1265, 1412
AclWI GGATC 4 cut(s) 258, 1677, 1690, 1773
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 3 cut(s) 395, 1033, 2035
AcuI CTGAAG 1 cut(s) 759
AfaI GTAC 3 cut(s) 736, 1574, 1867
AfiI CCNNNNNNNGG 4 cut(s) 45, 97, 1234, 1926
AgsI TTSAA 5 cut(s) 401, 479, 596, 686, 1378
AjiI CACGTC 2 cut(s) 1318, 1363
AjnI CCWGG 3 cut(s) 774, 1074, 2022
AluBI AGCT 6 cut(s) 235, 503, 641, 665, 1499, 1628
AluI AGCT 6 cut(s) 235, 503, 641, 665, 1499, 1628
Alw21I GWGCWC 8 cut(s) 237, 408, 486, 505, 537, 603, 667, 1171
Alw26I GTCTC 5 cut(s) 246, 1129, 1145, 1697, 1946
AlwI GGATC 4 cut(s) 258, 1677, 1690, 1773
Ama87I CYCGRG 2 cut(s) 236, 797
ApoI RAATTY 3 cut(s) 395, 1033, 2035
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
AspLEI GCGC 6 cut(s) 178, 289, 454, 1112, 1199, 1414
AspS9I GGNCC 2 cut(s) 1443, 1693
AvaI CYCGRG 2 cut(s) 236, 797
AvaII GGWCC 2 cut(s) 1443, 1693
BaeGI GKGCMC 1 cut(s) 1739
BamHI GGATCC 1 cut(s) 1682
BanI GGYRCC 3 cut(s) 73, 1736, 1916
BanII GRGCYC 4 cut(s) 237, 505, 556, 667
BarI GAAGNNNNNNTAC 2 cut(s) 16, 48
BbsI GAAGAC 2 cut(s) 1226, 1343
Bbv12I GWGCWC 8 cut(s) 237, 408, 486, 505, 537, 603, 667, 1171
BccI CCATC 1 cut(s) 997
BceAI ACGGC 2 cut(s) 207, 1191
BcgI CGANNNNNNTGC 2 cut(s) 1192, 1226
BciT130I CCWGG 3 cut(s) 776, 1076, 2024
BciVI GTATCC 1 cut(s) 1885
BclI TGATCA 3 cut(s) 295, 1813, 2047
BcoDI GTCTC 5 cut(s) 246, 1129, 1145, 1697, 1946
BfaI CTAG 3 cut(s) 756, 1458, 1581
BfmI CTRYAG 1 cut(s) 936
BfuAI ACCTGC 2 cut(s) 185, 1544
BfuI GTATCC 1 cut(s) 1885
BlpI GCTNAGC 1 cut(s) 1629
BmcAI AGTACT 2 cut(s) 736, 1867
Bme1390I CCNGG 3 cut(s) 776, 1076, 2024
Bme18I GGWCC 2 cut(s) 1443, 1693
BmeT110I CYCGRG 2 cut(s) 236, 797
BmgBI CACGTC 2 cut(s) 1318, 1363
BmgT120I GGNCC 2 cut(s) 1443, 1693
BmiI GGNNCC 6 cut(s) 75, 1444, 1684, 1694, 1738, 1918
BmrFI CCNGG 3 cut(s) 776, 1076, 2024
BmrI ACTGGG 1 cut(s) 1223
BmsI GCATC 2 cut(s) 749, 1528
BmuI ACTGGG 1 cut(s) 1223
BoxI GACNNNNGTC 2 cut(s) 1157, 1758
BpiI GAAGAC 2 cut(s) 1226, 1343
BplI GAGNNNNNCTC 4 cut(s) 917, 949, 1109, 1141
BpmI CTGGAG 2 cut(s) 663, 1423
Bpu1102I GCTNAGC 1 cut(s) 1629
BpuEI CTTGAG 5 cut(s) 230, 351, 489, 540, 1884
BsaAI YACGTR 1 cut(s) 1288
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 97, 1234, 1926
Bse118I RCCGGY 3 cut(s) 1355, 1907, 1919
Bse1I ACTGG 2 cut(s) 1229, 1711
BseBI CCWGG 3 cut(s) 776, 1076, 2024
BseLI CCNNNNNNNGG 4 cut(s) 45, 97, 1234, 1926
BseMII CTCAG 4 cut(s) 95, 1140, 1620, 1860
BseNI ACTGG 2 cut(s) 1229, 1711
BseRI GAGGAG 8 cut(s) 903, 1076, 1583, 1607, 1793, 1844, 2018, 2021
BseSI GKGCMC 1 cut(s) 1739
BseYI CCCAGC 1 cut(s) 1936
Bsh1236I CGCG 5 cut(s) 178, 454, 1244, 1265, 1412
Bsh1285I CGRYCG 1 cut(s) 1212
BshNI GGYRCC 3 cut(s) 73, 1736, 1916
BsiEI CGRYCG 1 cut(s) 1212
BsiHKAI GWGCWC 8 cut(s) 237, 408, 486, 505, 537, 603, 667, 1171
BsiHKCI CYCGRG 2 cut(s) 236, 797
BsiSI CCGG 3 cut(s) 1356, 1908, 1920
BslFI GGGAC 3 cut(s) 1281, 1456, 1706
BslI CCNNNNNNNGG 4 cut(s) 45, 97, 1234, 1926
BsmAI GTCTC 5 cut(s) 246, 1129, 1145, 1697, 1946
BsmFI GGGAC 3 cut(s) 1281, 1456, 1706
BsoBI CYCGRG 2 cut(s) 236, 797
Bsp1720I GCTNAGC 1 cut(s) 1629
Bsp19I CCATGG 1 cut(s) 1038
BspCNI CTCAG 4 cut(s) 96, 1139, 1621, 1861
BspFNI CGCG 5 cut(s) 178, 454, 1244, 1265, 1412
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 6 cut(s) 75, 1444, 1684, 1694, 1738, 1918
BspMI ACCTGC 2 cut(s) 185, 1544
BspPI GGATC 4 cut(s) 258, 1677, 1690, 1773
BspQI GCTCTTC 3 cut(s) 396, 474, 591
BspT107I GGYRCC 3 cut(s) 73, 1736, 1916
BsrFI RCCGGY 3 cut(s) 1355, 1907, 1919
BsrI ACTGG 2 cut(s) 1229, 1711
BssAI RCCGGY 3 cut(s) 1355, 1907, 1919
Bst2UI CCWGG 3 cut(s) 776, 1076, 2024
Bst4CI ACNGT 4 cut(s) 1210, 1393, 1465, 1741
BstBAI YACGTR 1 cut(s) 1288
BstC8I GCNNGC 7 cut(s) 146, 291, 639, 1201, 1399, 1921, 2060
BstDEI CTNAG 7 cut(s) 9, 104, 342, 1126, 1190, 1629, 1869
BstDSI CCRYGG 2 cut(s) 1038, 1263
BstEII GGTNACC 1 cut(s) 2018
BstENI CCTNNNNNAGG 1 cut(s) 43
BstFNI CGCG 5 cut(s) 178, 454, 1244, 1265, 1412
BstHHI GCGC 6 cut(s) 178, 289, 454, 1112, 1199, 1414
BstMAI GTCTC 5 cut(s) 246, 1129, 1145, 1697, 1946
BstMCI CGRYCG 1 cut(s) 1212
BstNI CCWGG 3 cut(s) 776, 1076, 2024
BstPAI GACNNNNGTC 2 cut(s) 1157, 1758
BstPI GGTNACC 1 cut(s) 2018
BstSCI CCNGG 3 cut(s) 774, 1074, 2022
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 1739
BstUI CGCG 5 cut(s) 178, 454, 1244, 1265, 1412
BstV2I GAAGAC 2 cut(s) 1226, 1343
BstX2I RGATCY 1 cut(s) 1682
BstXI CCANNNNNNTGG 1 cut(s) 518
BstYI RGATCY 1 cut(s) 1682
BsuI GTATCC 1 cut(s) 1885
BtgI CCRYGG 2 cut(s) 1038, 1263
BtrI CACGTC 2 cut(s) 1318, 1363
BtsI GCAGTG 2 cut(s) 1440, 1575
BtsIMutI CAGTG 2 cut(s) 1440, 1575
BveI ACCTGC 2 cut(s) 185, 1544
Cac8I GCNNGC 7 cut(s) 146, 291, 639, 1201, 1399, 1921, 2060
CciI TCATGA 1 cut(s) 229
CfoI GCGC 6 cut(s) 178, 289, 454, 1112, 1199, 1414
Cfr10I RCCGGY 3 cut(s) 1355, 1907, 1919
Cfr13I GGNCC 2 cut(s) 1443, 1693
Cfr42I CCGCGG 1 cut(s) 1266
CseI GACGC 1 cut(s) 443
Csp6I GTAC 3 cut(s) 735, 1573, 1866
CviAII CATG 7 cut(s) 164, 208, 230, 584, 1039, 1888, 1987
CviQI GTAC 3 cut(s) 735, 1573, 1866
DdeI CTNAG 7 cut(s) 9, 104, 342, 1126, 1190, 1629, 1869
EaeI YGGCCR 1 cut(s) 714
EciI GGCGGA 3 cut(s) 128, 338, 1493
Ecl136II GAGCTC 3 cut(s) 235, 503, 665
Eco24I GRGCYC 4 cut(s) 237, 505, 556, 667
Eco47I GGWCC 2 cut(s) 1443, 1693
Eco53kI GAGCTC 3 cut(s) 235, 503, 665
Eco57I CTGAAG 1 cut(s) 759
Eco88I CYCGRG 2 cut(s) 236, 797
Eco91I GGTNACC 1 cut(s) 2018
EcoICRI GAGCTC 3 cut(s) 235, 503, 665
EcoNI CCTNNNNNAGG 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 2018
EcoRI GAATTC 2 cut(s) 1033, 2035
EcoRII CCWGG 3 cut(s) 774, 1074, 2022
EcoT38I GRGCYC 4 cut(s) 237, 505, 556, 667
FaeI CATG 7 cut(s) 167, 211, 233, 587, 1042, 1891, 1990
FalI AAGNNNNNCTT 2 cut(s) 1173, 1205
FaqI GGGAC 3 cut(s) 1281, 1456, 1706
FatI CATG 7 cut(s) 163, 207, 229, 583, 1038, 1887, 1986
FauI CCCGC 4 cut(s) 1258, 1325, 1336, 1480
FauNDI CATATG 1 cut(s) 1351
FbaI TGATCA 3 cut(s) 295, 1813, 2047
FriOI GRGCYC 4 cut(s) 237, 505, 556, 667
FspBI CTAG 3 cut(s) 756, 1458, 1581
GlaI GCGC 6 cut(s) 177, 288, 453, 1111, 1198, 1413
GsaI CCCAGC 1 cut(s) 1940
GsuI CTGGAG 2 cut(s) 663, 1423
HapII CCGG 3 cut(s) 1356, 1908, 1920
HgaI GACGC 1 cut(s) 443
HhaI GCGC 6 cut(s) 178, 289, 454, 1112, 1199, 1414
Hin1II CATG 7 cut(s) 167, 211, 233, 587, 1042, 1891, 1990
Hin6I GCGC 6 cut(s) 176, 287, 452, 1110, 1197, 1412
HinP1I GCGC 6 cut(s) 176, 287, 452, 1110, 1197, 1412
HinfI GANTC 4 cut(s) 239, 1085, 1657, 1723
HpaII CCGG 3 cut(s) 1356, 1908, 1920
Hpy166II GTNNAC 1 cut(s) 1575
Hpy188I TCNGA 7 cut(s) 10, 43, 105, 1129, 1656, 1770, 1949
Hpy188III TCNNGA 6 cut(s) 85, 134, 230, 756, 1712, 1763
Hpy8I GTNNAC 1 cut(s) 1575
Hpy99I CGWCG 1 cut(s) 883
HpyCH4III ACNGT 4 cut(s) 1210, 1393, 1465, 1741
HpyCH4IV ACGT 4 cut(s) 1287, 1317, 1322, 1362
HpyCH4V TGCA 2 cut(s) 762, 1282
HpyF3I CTNAG 7 cut(s) 9, 104, 342, 1126, 1190, 1629, 1869
HpySE526I ACGT 4 cut(s) 1287, 1317, 1322, 1362
Hsp92II CATG 7 cut(s) 167, 211, 233, 587, 1042, 1891, 1990
HspAI GCGC 6 cut(s) 176, 287, 452, 1110, 1197, 1412
KroI GCCGGC 1 cut(s) 1919
KroNI GCCGGC 1 cut(s) 1921
Ksp22I TGATCA 3 cut(s) 295, 1813, 2047
KspI CCGCGG 1 cut(s) 1266
LguI GCTCTTC 3 cut(s) 396, 474, 591
LmnI GCTCC 6 cut(s) 449, 500, 1063, 1166, 1940, 2008
LweI GCATC 2 cut(s) 749, 1528
MaeI CTAG 3 cut(s) 756, 1458, 1581
MaeII ACGT 4 cut(s) 1287, 1317, 1322, 1362
MaeIII GTNAC 5 cut(s) 1318, 1358, 1641, 1853, 2018
MflI RGATCY 1 cut(s) 1682
MluCI AATT 3 cut(s) 395, 1033, 2035
MlyI GAGTC 3 cut(s) 248, 1651, 1732
MmeI TCCRAC 2 cut(s) 508, 1305
MroNI GCCGGC 1 cut(s) 1919
MspA1I CMGCKG 3 cut(s) 1265, 1499, 1628
MspI CCGG 3 cut(s) 1356, 1908, 1920
MspR9I CCNGG 3 cut(s) 776, 1076, 2024
MvaI CCWGG 3 cut(s) 776, 1076, 2024
MvnI CGCG 5 cut(s) 178, 454, 1244, 1265, 1412
NaeI GCCGGC 1 cut(s) 1921
NcoI CCATGG 1 cut(s) 1038
NdeI CATATG 1 cut(s) 1351
NgoMIV GCCGGC 1 cut(s) 1919
NlaIII CATG 7 cut(s) 167, 211, 233, 587, 1042, 1891, 1990
NlaIV GGNNCC 6 cut(s) 75, 1444, 1684, 1694, 1738, 1918
NmeAIII GCCGAG 2 cut(s) 361, 752
NmuCI GTSAC 4 cut(s) 1318, 1358, 1641, 1853
PaeR7I CTCGAG 2 cut(s) 236, 797
PagI TCATGA 1 cut(s) 229
PaqCI CACCTGC 2 cut(s) 185, 1544
PciSI GCTCTTC 3 cut(s) 396, 474, 591
PdiI GCCGGC 1 cut(s) 1921
PfeI GAWTC 1 cut(s) 1085
PleI GAGTC 3 cut(s) 247, 1651, 1731
PpsI GAGTC 3 cut(s) 247, 1651, 1731
Ppu21I YACGTR 1 cut(s) 1288
PshAI GACNNNNGTC 2 cut(s) 1157, 1758
Psp124BI GAGCTC 3 cut(s) 237, 505, 667
Psp6I CCWGG 3 cut(s) 774, 1074, 2022
PspEI GGTNACC 1 cut(s) 2018
PspFI CCCAGC 1 cut(s) 1936
PspGI CCWGG 3 cut(s) 774, 1074, 2022
PspN4I GGNNCC 6 cut(s) 75, 1444, 1684, 1694, 1738, 1918
PspPI GGNCC 2 cut(s) 1443, 1693
PspXI VCTCGAGB 2 cut(s) 236, 797
PsuI RGATCY 1 cut(s) 1682
PvuII CAGCTG 2 cut(s) 1499, 1628
RsaI GTAC 3 cut(s) 736, 1574, 1867
RsaNI GTAC 3 cut(s) 735, 1573, 1866
SacI GAGCTC 3 cut(s) 237, 505, 667
SacII CCGCGG 1 cut(s) 1266
SapI GCTCTTC 3 cut(s) 396, 474, 591
Sau96I GGNCC 2 cut(s) 1443, 1693
ScaI AGTACT 2 cut(s) 736, 1867
SchI GAGTC 3 cut(s) 248, 1651, 1732
ScrFI CCNGG 3 cut(s) 776, 1076, 2024
SfaNI GCATC 2 cut(s) 749, 1528
SfcI CTRYAG 1 cut(s) 936
Sfr274I CTCGAG 2 cut(s) 236, 797
Sfr303I CCGCGG 1 cut(s) 1266
SgrBI CCGCGG 1 cut(s) 1266
SinI GGWCC 2 cut(s) 1443, 1693
SlaI CTCGAG 2 cut(s) 236, 797
SmlI CTYRAG 7 cut(s) 236, 245, 366, 504, 555, 797, 1899
SmoI CTYRAG 7 cut(s) 236, 245, 366, 504, 555, 797, 1899
Sse9I AATT 3 cut(s) 395, 1033, 2035
SspMI CTAG 3 cut(s) 756, 1458, 1581
SstI GAGCTC 3 cut(s) 237, 505, 667
StyD4I CCNGG 3 cut(s) 774, 1074, 2022
TaaI ACNGT 4 cut(s) 1210, 1393, 1465, 1741
TaiI ACGT 4 cut(s) 1290, 1320, 1325, 1365
TaqI TCGA 2 cut(s) 237, 798
TasI AATT 3 cut(s) 395, 1033, 2035
TatI WGTACW 3 cut(s) 734, 1572, 1865
TauI GCSGC 6 cut(s) 363, 1412, 1433, 1457, 1478, 1547
TfiI GAWTC 1 cut(s) 1085
TscAI CASTG 2 cut(s) 1440, 1582
TseFI GTSAC 4 cut(s) 1318, 1358, 1641, 1853
Tsp45I GTSAC 4 cut(s) 1318, 1358, 1641, 1853
TspDTI ATGAA 2 cut(s) 854, 2028
TspRI CASTG 2 cut(s) 1440, 1582
VpaK11BI GGWCC 2 cut(s) 1443, 1693
XagI CCTNNNNNAGG 1 cut(s) 43
XapI RAATTY 3 cut(s) 395, 1033, 2035
XbaI TCTAGA 1 cut(s) 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 1703
XhoI CTCGAG 2 cut(s) 236, 797
XspI CTAG 3 cut(s) 756, 1458, 1581
ZrmI AGTACT 2 cut(s) 736, 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.