Rw2G027270

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001392
Physical Location & Seq
Reverse (-)
42603191 .. 42605674
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027270.1

Sequence Viewer

Length: 1956 bp
ATGTCTTCTGAGCAACTTAGGAGGGAGAATGTGACGCCCGAGAGGGAAGTTCACATTGAGAAAGACAAGGTGCCGAAGATCACCAGCCACTTCGAGTCAATCACCGTGCATGTGCAAGAAGCCGGCGACAAAGACACGCCTATGGACAGCACCAAAGACTCGCACGACGATAAAGAGCGGAAGGAAAACACCGTTGGGGACTTGGGGATGGGAAAGTCCAGAGAGACTAGTGAGATCGGGAGTCACAAGCTGGAGTCCCTCGCGGGACAAATGAAGGAGGCTGAGGAGAAGAGAGGGAGGGAGAGTGGTGCCCGCGGAGTAGGGAAGTTTGAGGTTCAAGAAAGTGATGAGGGTCAGAAGAAGGGAGGTAGAGGAGAGGGTAGAGAGGAAATGATTGTTGTGAGTGAAGGGAAGGAGGATTCAAGGGTGAGACAAGGTGGGAGTGAAATGGAAGGCAGAAACATGGACATAATGGGCAGAGAGAGTAAGAGTGGAGCTCAAGGTGTCGGAAAGCTCGAAGTTATGAGGGTTGATGAAGAGAAGGGCAGAGAGGGTCAAGGTAGACAGCAAATGAGTTCTAAAGGGAGGGAGGGCGAGGGTAGAGAAAGTGAAGGTGGGAGCACACAAATGATGGGCAAAGGAGAGGGTATGACACAAGAGAGAGGAATGGACACGGAGATGATGAGAAGCAAAGAGGAAAGTCGCGGCAGCCAGCAGAGACGAGGCACAGAAACGGAGAGCAATGTGAACAAGCAGCAGCAGCAACCACGACAACAACAACCATCTCTGGAAGAAGTTTCGGCATTTAGACAAACAGCACAGCAGAATTCCATGGCGGCAATAAGGGCGGCAGAAGAGCGATATCAGAAAGCTAAAGAAACTGCAAACCAGACACTCGATACTACAGCCGAGAAGACTAAAGAAACAGCCCAGAAGGCTAAAGAAACTGCAAGCCAGACACTAGGCCCTGCAGCCCAGAAAGCTAAAGAAACTGCCAGTACTGCTACCGAGAAGGCTAAAGAAATTTCAAGCCAGACACTTGGTACTGCAACTGAGAAAGGAGCACAAGTGAAAGACATAGCACTGGAAAAAGGCCAACAAGGATTATATGCAGCAAAAGATACTCTATCAAGTGCAGGGAAGAAAACGGTGGATTACACGGTGCCATTAGCAGAGAAAACTAAAGACATGGCCGTGTCTGCAGGTAAGACTACTCTGCATTATGCTGGTGATGTTGCGGTGGATTTGAAGGACAAGGCGACTGTGGCAGGTTGGACTGCGGCGCACTACACAACTGAGGCAGCAGTGGAGGGAACCAAAGCAGCTGCCAGAGTAGTTGCCGGAGCAGCAGGGTATGCTGGTCATAAGGCTGTGGATATTGTGTCCAAGCCTTTGACTGTTGCTAAAGATGCTGCTGTGGTGACTGGTGAGAAAGCTGAGGAGTTCACTGCTCGGAAGAAGGAAGAGGCGAAGAAAGAATATGAGGCCAAGAAAGCAGCTGAAGCAAATAGTCCCAAGGTAGATATTGGAATGGGTGAGGAGGGCCAGGAAGGGTGGAGCTCAAATGCACAGCAAGGAGGGCAGGAGGGGAGGTCATCTGAGAAGCAAGGCAGGCAAGAGGGGAGGTCATTTGAGCAGCAAGGCAAGCAGAGCGAGACTGTAAGTGTAATACCGGGAGCTGTCGGCGAAACTTTGATTGAGATAGCTCAGACTACAAAAGACAACGTCATCGGGCAGGGCCAAAGCGCAGAGGAGAGTGCTGAGAAGGTTCGCAGGTCATCTGATCAGCAGCAAGGCTTAGGTGCAATACCTCGTGCTGTTGGGGAGACTATGGTTGGAATAGCTCAGACAACAAAAGACAATGTTGACAATATTACTGGGCTTAGGCGGGGACGGGGACAGGGACAGTCGAGAACTGATCAGCAGCAGGGATGGAAGCAAGACCAGACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

651

Amino Acids

70.25

Weight (kDa)

5.85

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1193, 1259, 1764
AccB1I GGYRCC 3 cut(s) 70, 308, 1162
AccBSI CCGCTC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 562
AccII CGCG 3 cut(s) 263, 315, 705
AcoI YGGCCR 1 cut(s) 1191
AcsI RAATTY 2 cut(s) 826, 1023
AcuI CTGAAG 1 cut(s) 1521
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 2 cut(s) 1000, 1045
AgsI TTSAA 4 cut(s) 338, 423, 1029, 1249
AhlI ACTAGT 1 cut(s) 227
AjnI CCWGG 1 cut(s) 1545
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
Alw21I GWGCWC 4 cut(s) 499, 623, 1066, 1562
Alw26I GTCTC 5 cut(s) 218, 424, 712, 1649, 1820
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 6 cut(s) 964, 1093, 1191, 1485, 1543, 1738
ApoI RAATTY 2 cut(s) 826, 1023
AspLEI GCGC 2 cut(s) 1285, 1748
AspS9I GGNCC 3 cut(s) 965, 1543, 1738
AsuC2I CCSGG 1 cut(s) 1674
AsuHPI GGTGA 7 cut(s) 73, 94, 439, 1241, 1432, 1439, 1547
AvaI CYCGRG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 313
BanI GGYRCC 3 cut(s) 70, 308, 1162
BanII GRGCYC 2 cut(s) 499, 1562
BauI CACGAG 1 cut(s) 1812
BbsI GAAGAC 1 cut(s) 920
Bbv12I GWGCWC 4 cut(s) 499, 623, 1066, 1562
BbvCI CCTCAGC 2 cut(s) 282, 1437
BccI CCATC 4 cut(s) 202, 625, 790, 1926
BceAI ACGGC 1 cut(s) 1178
BciT130I CCWGG 1 cut(s) 1547
BclI TGATCA 2 cut(s) 1783, 1918
BcnI CCSGG 1 cut(s) 1674
BcoDI GTCTC 5 cut(s) 218, 424, 712, 1649, 1820
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 2 cut(s) 228, 962
BfmI CTRYAG 3 cut(s) 903, 969, 1200
BfuAI ACCTGC 3 cut(s) 1193, 1259, 1764
BglI GCCNNNNNGGC 1 cut(s) 935
BmcAI AGTACT 1 cut(s) 1000
Bme1390I CCNGG 2 cut(s) 1547, 1674
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 965, 1543, 1738
BmiI GGNNCC 4 cut(s) 72, 310, 1164, 1315
BmrFI CCNGG 2 cut(s) 1547, 1674
BmrI ACTGGG 1 cut(s) 1887
BmsI GCATC 1 cut(s) 1399
BmuI ACTGGG 1 cut(s) 1887
BpiI GAAGAC 1 cut(s) 920
BplI GAGNNNNNCTC 2 cut(s) 481, 513
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 4 cut(s) 282, 1437, 1798, 1883
BpuEI CTTGAG 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 1674
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 313, 831, 1515
BsaXI ACNNNNNCTCC 6 cut(s) 289, 319, 1053, 1083, 1577, 1607
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 4 cut(s) 996, 1089, 1429, 1882
Bse3DI GCAATG 1 cut(s) 748
BseBI CCWGG 1 cut(s) 1547
BseDI CCNNGG 3 cut(s) 313, 831, 1515
BseGI GGATG 2 cut(s) 213, 1937
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 8 cut(s) 273, 1044, 1287, 1428, 1590, 1721, 1752, 1859
BseNI ACTGG 4 cut(s) 996, 1089, 1429, 1882
BseRI GAGGAG 5 cut(s) 299, 387, 1454, 1553, 1766
BseSI GKGCMC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 1155
Bsh1236I CGCG 3 cut(s) 263, 315, 705
BshFI GGCC 6 cut(s) 966, 1095, 1193, 1487, 1545, 1740
BshNI GGYRCC 3 cut(s) 70, 308, 1162
BsiHKAI GWGCWC 4 cut(s) 499, 623, 1066, 1562
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 3 cut(s) 123, 1341, 1673
BslFI GGGAC 7 cut(s) 212, 241, 279, 1497, 1905, 1911, 1917
BsmAI GTCTC 5 cut(s) 218, 424, 712, 1649, 1820
BsmBI CGTCTC 1 cut(s) 712
BsmFI GGGAC 7 cut(s) 212, 241, 279, 1497, 1905, 1911, 1917
BsnI GGCC 6 cut(s) 966, 1095, 1193, 1487, 1545, 1740
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 5 cut(s) 313, 499, 623, 1066, 1562
Bsp143I GATC 4 cut(s) 78, 234, 1783, 1918
Bsp19I CCATGG 1 cut(s) 831
BspANI GGCC 6 cut(s) 966, 1095, 1193, 1487, 1545, 1740
BspCNI CTCAG 8 cut(s) 274, 1045, 1288, 1429, 1591, 1720, 1753, 1858
BspFNI CGCG 3 cut(s) 263, 315, 705
BspLI GGNNCC 4 cut(s) 72, 310, 1164, 1315
BspMAI CTGCAG 2 cut(s) 973, 1204
BspMI ACCTGC 3 cut(s) 1193, 1259, 1764
BspQI GCTCTTC 1 cut(s) 849
BspT107I GGYRCC 3 cut(s) 70, 308, 1162
BsrBI CCGCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 4 cut(s) 996, 1089, 1429, 1882
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 3 cut(s) 313, 831, 1515
BssMI GATC 4 cut(s) 78, 234, 1783, 1918
BssNI GRCGYC 1 cut(s) 35
BssSI CACGAG 1 cut(s) 1812
BssT1I CCWWGG 2 cut(s) 831, 1515
Bst2BI CACGAG 1 cut(s) 1812
Bst2UI CCWGG 1 cut(s) 1547
Bst4CI ACNGT 8 cut(s) 106, 193, 1150, 1162, 1264, 1399, 1660, 1908
Bst6I CTCTTC 4 cut(s) 284, 531, 849, 1458
BstACI GRCGYC 1 cut(s) 35
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstC8I GCNNGC 6 cut(s) 124, 313, 713, 952, 1613, 1646
BstDSI CCRYGG 2 cut(s) 313, 831
BstF5I GGATG 2 cut(s) 213, 1937
BstFNI CGCG 3 cut(s) 263, 315, 705
BstHHI GCGC 2 cut(s) 1285, 1748
BstKTI GATC 4 cut(s) 81, 237, 1786, 1921
BstMAI GTCTC 5 cut(s) 218, 424, 712, 1649, 1820
BstMBI GATC 4 cut(s) 78, 234, 1783, 1918
BstNI CCWGG 1 cut(s) 1547
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 1545, 1672
BstSFI CTRYAG 3 cut(s) 903, 969, 1200
BstSLI GKGCMC 1 cut(s) 313
BstUI CGCG 3 cut(s) 263, 315, 705
BstV2I GAAGAC 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 1040
BsuRI GGCC 6 cut(s) 966, 1095, 1193, 1487, 1545, 1740
BtgI CCRYGG 2 cut(s) 313, 831
BtsCI GGATG 2 cut(s) 213, 1937
BtsI GCAGTG 2 cut(s) 1311, 1446
BtsIMutI CAGTG 3 cut(s) 1082, 1311, 1446
BveI ACCTGC 3 cut(s) 1193, 1259, 1764
Cac8I GCNNGC 6 cut(s) 124, 313, 713, 952, 1613, 1646
CfoI GCGC 2 cut(s) 1285, 1748
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 965, 1543, 1738
Cfr42I CCGCGG 1 cut(s) 316
CseI GACGC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 999, 1044
CviAII CATG 4 cut(s) 110, 463, 832, 1189
CviQI GTAC 2 cut(s) 999, 1044
DpnI GATC 4 cut(s) 80, 236, 1785, 1920
DpnII GATC 4 cut(s) 78, 234, 1783, 1918
EaeI YGGCCR 1 cut(s) 1191
Eam1104I CTCTTC 4 cut(s) 284, 531, 849, 1458
EarI CTCTTC 4 cut(s) 284, 531, 849, 1458
Ecl136II GAGCTC 2 cut(s) 497, 1560
Eco130I CCWWGG 2 cut(s) 831, 1515
Eco24I GRGCYC 2 cut(s) 499, 1562
Eco32I GATATC 1 cut(s) 863
Eco53kI GAGCTC 2 cut(s) 497, 1560
Eco57I CTGAAG 1 cut(s) 1521
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 2 cut(s) 497, 1560
EcoO109I RGGNCCY 1 cut(s) 965
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 1545
EcoRV GATATC 1 cut(s) 863
EcoT14I CCWWGG 2 cut(s) 831, 1515
EcoT38I GRGCYC 2 cut(s) 499, 1562
ErhI CCWWGG 2 cut(s) 831, 1515
Esp3I CGTCTC 1 cut(s) 712
FaeI CATG 4 cut(s) 113, 466, 835, 1192
FaqI GGGAC 7 cut(s) 212, 241, 279, 1497, 1905, 1911, 1917
FatI CATG 4 cut(s) 109, 462, 831, 1188
FauI CCCGC 3 cut(s) 256, 320, 1881
FbaI TGATCA 2 cut(s) 1783, 1918
FblI GTMKAC 1 cut(s) 562
FokI GGATG 2 cut(s) 220, 1944
FriOI GRGCYC 2 cut(s) 499, 1562
FspBI CTAG 2 cut(s) 228, 962
GlaI GCGC 2 cut(s) 1284, 1747
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 6 cut(s) 966, 1095, 1193, 1487, 1545, 1740
HapII CCGG 3 cut(s) 123, 1341, 1673
HgaI GACGC 1 cut(s) 43
HhaI GCGC 2 cut(s) 1285, 1748
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 4 cut(s) 113, 466, 835, 1192
Hin6I GCGC 2 cut(s) 1283, 1746
HinP1I GCGC 2 cut(s) 1283, 1746
HincII GTYRAC 1 cut(s) 1867
HindII GTYRAC 1 cut(s) 1867
HinfI GANTC 5 cut(s) 95, 158, 241, 254, 419
HpaII CCGG 3 cut(s) 123, 1341, 1673
HphI GGTGA 7 cut(s) 73, 94, 439, 1241, 1432, 1439, 1547
Hpy166II GTNNAC 5 cut(s) 52, 563, 748, 1446, 1867
Hpy188I TCNGA 9 cut(s) 10, 357, 509, 867, 1455, 1600, 1710, 1783, 1848
Hpy188III TCNNGA 5 cut(s) 219, 238, 338, 788, 1911
Hpy8I GTNNAC 5 cut(s) 52, 563, 748, 1446, 1867
Hpy99I CGWCG 1 cut(s) 170
HpyCH4III ACNGT 8 cut(s) 106, 193, 1150, 1162, 1264, 1399, 1660, 1908
HpyCH4IV ACGT 1 cut(s) 1725
HpySE526I ACGT 1 cut(s) 1725
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 4 cut(s) 113, 466, 835, 1192
HspAI GCGC 2 cut(s) 1283, 1746
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Ksp22I TGATCA 2 cut(s) 1783, 1918
KspI CCGCGG 1 cut(s) 316
Kzo9I GATC 4 cut(s) 78, 234, 1783, 1918
LguI GCTCTTC 1 cut(s) 849
LmnI GCTCC 6 cut(s) 494, 618, 1061, 1343, 1557, 1676
LweI GCATC 1 cut(s) 1399
MaeI CTAG 2 cut(s) 228, 962
MaeII ACGT 1 cut(s) 1725
MaeIII GTNAC 3 cut(s) 31, 242, 1420
MalI GATC 4 cut(s) 80, 236, 1785, 1920
MbiI CCGCTC 1 cut(s) 178
MboI GATC 4 cut(s) 78, 234, 1783, 1918
MhlI GDGCHC 5 cut(s) 313, 499, 623, 1066, 1562
MluCI AATT 2 cut(s) 826, 1023
MlyI GAGTC 4 cut(s) 104, 152, 250, 263
MmeI TCCRAC 3 cut(s) 487, 1253, 1816
MroNI GCCGGC 1 cut(s) 122
MslI CAYNNNNRTG 4 cut(s) 140, 626, 677, 1193
MspA1I CMGCKG 3 cut(s) 315, 1325, 1499
MspI CCGG 3 cut(s) 123, 1341, 1673
MspR9I CCNGG 2 cut(s) 1547, 1674
MvaI CCWGG 1 cut(s) 1547
MvnI CGCG 3 cut(s) 263, 315, 705
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 1674
NcoI CCATGG 1 cut(s) 831
NdeII GATC 4 cut(s) 78, 234, 1783, 1918
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 4 cut(s) 113, 466, 835, 1192
NlaIV GGNNCC 4 cut(s) 72, 310, 1164, 1315
NmeAIII GCCGAG 1 cut(s) 934
NmuCI GTSAC 3 cut(s) 31, 242, 1420
NspI RCATGY 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 419
PflFI GACNNNGTC 1 cut(s) 1724
PleI GAGTC 4 cut(s) 103, 152, 249, 262
PpsI GAGTC 4 cut(s) 103, 152, 249, 262
Psp124BI GAGCTC 2 cut(s) 499, 1562
Psp6I CCWGG 1 cut(s) 1545
PspGI CCWGG 1 cut(s) 1545
PspN4I GGNNCC 4 cut(s) 72, 310, 1164, 1315
PspPI GGNCC 3 cut(s) 965, 1543, 1738
PstI CTGCAG 2 cut(s) 973, 1204
PsyI GACNNNGTC 1 cut(s) 1724
PvuII CAGCTG 2 cut(s) 1325, 1499
RsaI GTAC 2 cut(s) 1000, 1045
RsaNI GTAC 2 cut(s) 999, 1044
RseI CAYNNNNRTG 4 cut(s) 140, 626, 677, 1193
SacI GAGCTC 2 cut(s) 499, 1562
SacII CCGCGG 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 849
Sau3AI GATC 4 cut(s) 78, 234, 1783, 1918
Sau96I GGNCC 3 cut(s) 965, 1543, 1738
ScaI AGTACT 1 cut(s) 1000
SchI GAGTC 4 cut(s) 104, 152, 250, 263
ScrFI CCNGG 2 cut(s) 1547, 1674
SduI GDGCHC 5 cut(s) 313, 499, 623, 1066, 1562
SfaNI GCATC 1 cut(s) 1399
SfcI CTRYAG 3 cut(s) 903, 969, 1200
Sfr303I CCGCGG 1 cut(s) 316
SgrBI CCGCGG 1 cut(s) 316
SmiMI CAYNNNNRTG 4 cut(s) 140, 626, 677, 1193
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
SpeI ACTAGT 1 cut(s) 227
Sse9I AATT 2 cut(s) 826, 1023
SspI AATATT 1 cut(s) 1873
SspMI CTAG 2 cut(s) 228, 962
SstI GAGCTC 2 cut(s) 499, 1562
StyD4I CCNGG 2 cut(s) 1545, 1672
StyI CCWWGG 2 cut(s) 831, 1515
TaaI ACNGT 8 cut(s) 106, 193, 1150, 1162, 1264, 1399, 1660, 1908
TaiI ACGT 1 cut(s) 1728
TaqI TCGA 4 cut(s) 93, 516, 897, 1910
TasI AATT 2 cut(s) 826, 1023
TatI WGTACW 1 cut(s) 998
TauI GCSGC 4 cut(s) 708, 839, 851, 1283
TfiI GAWTC 1 cut(s) 419
TscAI CASTG 3 cut(s) 1089, 1311, 1453
TseFI GTSAC 3 cut(s) 31, 242, 1420
Tsp45I GTSAC 3 cut(s) 31, 242, 1420
TspDTI ATGAA 2 cut(s) 287, 549
TspGWI ACGGA 2 cut(s) 689, 749
TspRI CASTG 3 cut(s) 1089, 1311, 1453
Tth111I GACNNNGTC 1 cut(s) 1724
XapI RAATTY 2 cut(s) 826, 1023
XceI RCATGY 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 562
XspI CTAG 2 cut(s) 228, 962
ZrmI AGTACT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.