FvH4_6g49010

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000641
Physical Location & Seq
Forward (+)
36801384 .. 36807140
5757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49010.t5

Sequence Viewer

Length: 1524 bp
ATGGGAGTCGGGGTTGAGGCTTTGCAGGTTGGGAGTTGGAACAAGGGAAAATCAAAGGGGAAGCATAAGATTGAGGAGGGAGATGAAATTAAAGGTTGTTGGATGAAGTTTAGGTTCATGGGGAGATGCTTGTCTCCAAAGACCAAAATGGATAATTCTACCAGTGGAACTAGCACACAATATGAAGATAGCAAGTCTACAAATGACACAATCTACAACCATGCAGTAGCTCCAGGTGCTTTGTCTTCAACTACTAGTAACACAGAAAGTGCTTTGTCCACTCCCAAAGCACCTGAGGAACTGATAGTTGCTTCTCAGCTTCGAAGATTCACCTTCAATGAGCTTAAGTCAGCAGCAAAGAATTTTAGACCTGAGAATCTTTTGGGGGAAGGCGGATTTGGCTGTGTATACAAAGGCTGGATCAGTGAGACTGGAACGTCTCCAACAAAACCTGGTATCGGGCTTCCCGTCGCTGTAAAGACTCTGAACCATGATGGACTTCAGGGCCACAAAGAATGGATGGCTGAAGTTATTTATCTTGGTGAATTGCTCCATCCTAACTTAGTCAAATTAGTTGGATATGGCATCGAGGATGATAAAAGATTACTTGTGTATGAGTTTATGCCTCGAGGGAGCTTGGAGAATCACCTCTTTAGAAGGAACTTGCCTCTTCCTTGGTCCATCAGAATGAAAATAGCTCTTGGTGCTGCAAAGGGTCTTGCATATCTTCATGAGGAATCTGAAAGACCGGTGATTTTTCGAGATTTTAAAACATCTAACATCTTATTAGATGCGGACTACAACGCCAAGCTTTCTGATTTTGGGCTCGCTAAAGATGGTCCAGAGGGAGAAAAAACCCATGTCTCCACCCGAGTGATGGGCACTTACGGTTATGCGGCACCCGAGTATGTTATGACAGGTCACCTTACTTCAAAAAGTGATGTATATAGTTTTGGGGTGGTTCTGCTTGAATTACTGATTGGACGAAGATCTATGGACAAAAATCGACCCCAAGGAGAGCACAACTTGGTGGAGTGGGCACGCCCACATCTTGGAGATAGGCGAAGATACTACCGACTAATAGATCCTCGACTTGAAGGTCGCTTCTCAATTAAAGGTGCTGAAATGGCATTTCAGTTGGCTGCCCACTGTCTTAGTCGTAACCCAAAGGCTAGACCTCTTATGAGTGAAGTTGTTCAAGTGATCAAGCCTCTGCTGCACCTCAAGGATCAAGCATATTCCTCATCTCACCCCCAAGGCATGCACACAGAGCGTCCTGGTTCCAGTCCCAACTTTAGAAATGGCCTTTGGGTTCCGGCAAGGAATGGACAACCATCTAGAAGTCAGTCTCTACCAAATGGTCCCAATGTCTCACCATATCACCTTAGCCATCCTCACCGCTCTCTACCAAATGGTCCCAATGTCTCACCATATCACCTTAACCATCCTCACCGATCACCCAAACCATCTACAGCTAAGTCCCCCAAAACTACACCAAAATCACCCAAACCTGCTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

508

Amino Acids

56.26

Weight (kDa)

9.58

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 124 - 399 1.1e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 125 - 399 5.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 436, 1098
AbsI CCTCGAGG 1 cut(s) 627
Acc36I ACCTGC 2 cut(s) 16, 1519
AccB1I GGYRCC 1 cut(s) 898
AccB7I CCANNNNNTGG 1 cut(s) 1052
AccBSI CCGCTC 1 cut(s) 1401
AccI GTMKAC 2 cut(s) 197, 408
AciI CCGC 4 cut(s) 393, 794, 896, 1399
AclWI GGATC 3 cut(s) 428, 1079, 1236
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 2 cut(s) 485, 546
AfiI CCNNNNNNNGG 3 cut(s) 458, 877, 1052
AflII CTTAAG 1 cut(s) 344
AgeI ACCGGT 1 cut(s) 748
AgsI TTSAA 6 cut(s) 249, 337, 933, 971, 1097, 1199
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 3 cut(s) 232, 451, 1276
AjuI GAANNNNNNNTTGG 6 cut(s) 381, 413, 963, 995, 1291, 1323
AleI CACNNNNGTG 1 cut(s) 872
AluBI AGCT 7 cut(s) 230, 319, 343, 636, 698, 811, 1475
AluI AGCT 7 cut(s) 230, 319, 343, 636, 698, 811, 1475
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 7 cut(s) 138, 422, 444, 868, 1353, 1375, 1429
AlwI GGATC 3 cut(s) 428, 1079, 1236
Ama87I CYCGRG 3 cut(s) 627, 870, 902
AoxI GGCC 2 cut(s) 505, 1303
ApeKI GCWGC 4 cut(s) 353, 707, 1142, 1216
ApoI RAATTY 1 cut(s) 361
AsiGI ACCGGT 1 cut(s) 748
Asp700I GAANNNNTTC 1 cut(s) 1194
AspS9I GGNCC 5 cut(s) 505, 678, 839, 1361, 1415
AsuII TTCGAA 1 cut(s) 322
AvaI CYCGRG 3 cut(s) 627, 870, 902
AvaII GGWCC 4 cut(s) 678, 839, 1361, 1415
AxyI CCTNAGG 1 cut(s) 294
BaeGI GKGCMC 2 cut(s) 884, 1042
BanI GGYRCC 1 cut(s) 898
BanII GRGCYC 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 1023
BbvI GCAGC 4 cut(s) 365, 694, 1129, 1203
BciT130I CCWGG 3 cut(s) 234, 453, 1278
BclI TGATCA 1 cut(s) 1203
BcoDI GTCTC 7 cut(s) 138, 422, 444, 868, 1353, 1375, 1429
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 4 cut(s) 171, 255, 1173, 1338
BfmI CTRYAG 1 cut(s) 1470
BfrI CTTAAG 1 cut(s) 344
BfuAI ACCTGC 2 cut(s) 16, 1519
BglII AGATCT 1 cut(s) 989
BisI GCNGC 5 cut(s) 354, 708, 897, 1143, 1217
BlsI GCNGC 5 cut(s) 355, 709, 898, 1144, 1218
Bme1390I CCNGG 3 cut(s) 234, 453, 1278
Bme18I GGWCC 4 cut(s) 678, 839, 1361, 1415
BmeT110I CYCGRG 3 cut(s) 627, 870, 902
BmgT120I GGNCC 5 cut(s) 505, 678, 839, 1361, 1415
BmiI GGNNCC 5 cut(s) 900, 1282, 1314, 1363, 1417
BmrFI CCNGG 3 cut(s) 234, 453, 1278
BmsI GCATC 3 cut(s) 116, 594, 781
BpiI GAAGAC 1 cut(s) 237
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 1385
Bpu14I TTCGAA 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 1208
BsaJI CCNNGG 3 cut(s) 674, 1012, 1255
BsaWI WCCGGW 1 cut(s) 748
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 458, 877, 1052
Bse118I RCCGGY 1 cut(s) 748
Bse1I ACTGG 3 cut(s) 162, 436, 1284
Bse21I CCTNAGG 1 cut(s) 294
BseBI CCWGG 3 cut(s) 234, 453, 1278
BseDI CCNNGG 3 cut(s) 674, 1012, 1255
BseGI GGATG 6 cut(s) 108, 525, 553, 598, 1390, 1444
BseLI CCNNNNNNNGG 3 cut(s) 458, 877, 1052
BseMII CTCAG 3 cut(s) 285, 329, 363
BseNI ACTGG 3 cut(s) 162, 436, 1284
BseRI GAGGAG 1 cut(s) 89
BseSI GKGCMC 2 cut(s) 884, 1042
BseXI GCAGC 4 cut(s) 365, 694, 1129, 1203
BsgI GTGCAG 1 cut(s) 1202
BshFI GGCC 2 cut(s) 507, 1305
BshNI GGYRCC 1 cut(s) 898
BshTI ACCGGT 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 3 cut(s) 627, 870, 902
BsiSI CCGG 2 cut(s) 749, 1316
BslFI GGGAC 4 cut(s) 1272, 1347, 1401, 1465
BslI CCNNNNNNNGG 3 cut(s) 458, 877, 1052
BsmAI GTCTC 7 cut(s) 138, 422, 444, 868, 1353, 1375, 1429
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 4 cut(s) 1272, 1347, 1401, 1465
BsnI GGCC 2 cut(s) 507, 1305
BsoBI CYCGRG 3 cut(s) 627, 870, 902
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 4 cut(s) 828, 884, 1023, 1042
Bsp143I GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
BspACI CCGC 4 cut(s) 393, 794, 896, 1399
BspANI GGCC 2 cut(s) 507, 1305
BspCNI CTCAG 3 cut(s) 286, 328, 364
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 5 cut(s) 900, 1282, 1314, 1363, 1417
BspMI ACCTGC 2 cut(s) 16, 1519
BspPI GGATC 3 cut(s) 428, 1079, 1236
BspT104I TTCGAA 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 898
BspTI CTTAAG 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 1401
BsrFI RCCGGY 1 cut(s) 748
BsrI ACTGG 3 cut(s) 162, 436, 1284
BssAI RCCGGY 1 cut(s) 748
BssECI CCNNGG 3 cut(s) 674, 1012, 1255
BssMI GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
BssNAI GTATAC 1 cut(s) 409
BssT1I CCWWGG 3 cut(s) 674, 1012, 1255
Bst1107I GTATAC 1 cut(s) 409
Bst2UI CCWGG 3 cut(s) 234, 453, 1278
Bst4CI ACNGT 2 cut(s) 890, 1151
Bst6I CTCTTC 1 cut(s) 675
BstAFI CTTAAG 1 cut(s) 344
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 3 cut(s) 828, 1042, 1262
BstDEI CTNAG 7 cut(s) 294, 315, 372, 562, 1154, 1385, 1476
BstEII GGTNACC 1 cut(s) 920
BstF5I GGATG 6 cut(s) 108, 525, 553, 598, 1390, 1444
BstKTI GATC 6 cut(s) 423, 992, 1087, 1206, 1231, 1457
BstMAI GTCTC 7 cut(s) 138, 422, 444, 868, 1353, 1375, 1429
BstMBI GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
BstMWI GCNNNNNNNGC 6 cut(s) 236, 399, 704, 1127, 1216, 1270
BstNI CCWGG 3 cut(s) 234, 453, 1278
BstNSI RCATGY 1 cut(s) 1264
BstPI GGTNACC 1 cut(s) 920
BstSCI CCNGG 3 cut(s) 232, 451, 1276
BstSFI CTRYAG 1 cut(s) 1470
BstSLI GKGCMC 2 cut(s) 884, 1042
BstV1I GCAGC 4 cut(s) 365, 694, 1129, 1203
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 2 cut(s) 989, 1084
BstYI RGATCY 2 cut(s) 989, 1084
BstZ17I GTATAC 1 cut(s) 409
Bsu36I CCTNAGG 1 cut(s) 294
BsuRI GGCC 2 cut(s) 507, 1305
BtsCI GGATG 6 cut(s) 108, 525, 553, 598, 1390, 1444
BtsIMutI CAGTG 3 cut(s) 169, 430, 1147
BveI ACCTGC 2 cut(s) 16, 1519
Cac8I GCNNGC 3 cut(s) 828, 1042, 1262
CciI TCATGA 1 cut(s) 730
Cfr10I RCCGGY 1 cut(s) 748
Cfr13I GGNCC 5 cut(s) 505, 678, 839, 1361, 1415
CseI GACGC 1 cut(s) 1262
CsiI ACCWGGT 1 cut(s) 451
CspAI ACCGGT 1 cut(s) 748
CviAII CATG 6 cut(s) 118, 221, 491, 731, 860, 1261
DdeI CTNAG 7 cut(s) 294, 315, 372, 562, 1154, 1385, 1476
DpnI GATC 6 cut(s) 422, 991, 1086, 1205, 1230, 1456
DpnII GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
DraI TTTAAA 1 cut(s) 769
DrdI GACNNNNNNGTC 2 cut(s) 436, 1098
DseDI GACNNNNNNGTC 2 cut(s) 436, 1098
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
EciI GGCGGA 1 cut(s) 408
Eco130I CCWWGG 3 cut(s) 674, 1012, 1255
Eco24I GRGCYC 1 cut(s) 828
Eco47I GGWCC 4 cut(s) 678, 839, 1361, 1415
Eco57I CTGAAG 2 cut(s) 485, 546
Eco81I CCTNAGG 1 cut(s) 294
Eco88I CYCGRG 3 cut(s) 627, 870, 902
Eco91I GGTNACC 1 cut(s) 920
EcoO65I GGTNACC 1 cut(s) 920
EcoRII CCWGG 3 cut(s) 232, 451, 1276
EcoT14I CCWWGG 3 cut(s) 674, 1012, 1255
EcoT38I GRGCYC 1 cut(s) 828
ErhI CCWWGG 3 cut(s) 674, 1012, 1255
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 6 cut(s) 121, 224, 494, 734, 863, 1264
FaqI GGGAC 4 cut(s) 1272, 1347, 1401, 1465
FatI CATG 6 cut(s) 117, 220, 490, 730, 859, 1260
FbaI TGATCA 1 cut(s) 1203
FblI GTMKAC 2 cut(s) 197, 408
Fnu4HI GCNGC 5 cut(s) 354, 708, 897, 1143, 1217
FokI GGATG 6 cut(s) 115, 532, 540, 605, 1377, 1431
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 5 cut(s) 354, 708, 897, 1143, 1217
FspBI CTAG 4 cut(s) 171, 255, 1173, 1338
GluI GCNGC 5 cut(s) 354, 708, 897, 1143, 1217
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 507, 1305
HapII CCGG 2 cut(s) 749, 1316
HgaI GACGC 1 cut(s) 1262
Hin1II CATG 6 cut(s) 121, 224, 494, 734, 863, 1264
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 6 cut(s) 6, 327, 376, 481, 643, 737
HpaII CCGG 2 cut(s) 749, 1316
Hpy166II GTNNAC 3 cut(s) 198, 279, 409
Hpy188I TCNGA 4 cut(s) 486, 686, 742, 817
Hpy188III TCNNGA 4 cut(s) 731, 761, 842, 1338
Hpy8I GTNNAC 3 cut(s) 198, 279, 409
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 4 cut(s) 343, 383, 651, 1091
HpyCH4III ACNGT 2 cut(s) 890, 1151
HpyCH4IV ACGT 1 cut(s) 437
HpyCH4V TGCA 6 cut(s) 25, 224, 710, 722, 1219, 1264
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 399, 704, 1127, 1216, 1270
HpyF3I CTNAG 7 cut(s) 294, 315, 372, 562, 1154, 1385, 1476
HpySE526I ACGT 1 cut(s) 437
Hsp92II CATG 6 cut(s) 121, 224, 494, 734, 863, 1264
Ksp22I TGATCA 1 cut(s) 1203
Kzo9I GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
LmnI GCTCC 3 cut(s) 235, 555, 633
Lsp1109I GCAGC 4 cut(s) 365, 694, 1129, 1203
LweI GCATC 3 cut(s) 116, 594, 781
MabI ACCWGGT 1 cut(s) 451
MaeI CTAG 4 cut(s) 171, 255, 1173, 1338
MaeII ACGT 1 cut(s) 437
MaeIII GTNAC 3 cut(s) 257, 920, 1160
MalI GATC 6 cut(s) 422, 991, 1086, 1205, 1230, 1456
MbiI CCGCTC 1 cut(s) 1401
MboI GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
MboII GAAGA 7 cut(s) 197, 237, 336, 662, 719, 999, 1077
MflI RGATCY 2 cut(s) 989, 1084
MhlI GDGCHC 4 cut(s) 828, 884, 1023, 1042
MluCI AATT 7 cut(s) 87, 154, 361, 545, 569, 971, 1110
MlyI GAGTC 2 cut(s) 15, 475
MmeI TCCRAC 4 cut(s) 17, 80, 467, 556
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 5 cut(s) 90, 345, 768, 1113, 1440
MslI CAYNNNNRTG 2 cut(s) 686, 872
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 2 cut(s) 749, 1316
MspR9I CCNGG 3 cut(s) 234, 453, 1278
MvaI CCWGG 3 cut(s) 234, 453, 1278
MwoI GCNNNNNNNGC 6 cut(s) 236, 399, 704, 1127, 1216, 1270
NdeII GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
NlaIII CATG 6 cut(s) 121, 224, 494, 734, 863, 1264
NlaIV GGNNCC 5 cut(s) 900, 1282, 1314, 1363, 1417
NmuCI GTSAC 1 cut(s) 920
NspI RCATGY 1 cut(s) 1264
NspV TTCGAA 1 cut(s) 322
OliI CACNNNNGTG 1 cut(s) 872
PaeI GCATGC 1 cut(s) 1264
PaeR7I CTCGAG 1 cut(s) 627
PagI TCATGA 1 cut(s) 730
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 4 cut(s) 327, 376, 643, 737
PflMI CCANNNNNTGG 1 cut(s) 1052
PinAI ACCGGT 1 cut(s) 748
PkrI GCNGC 5 cut(s) 355, 709, 898, 1144, 1218
PleI GAGTC 2 cut(s) 14, 475
PpsI GAGTC 2 cut(s) 14, 475
Psp6I CCWGG 3 cut(s) 232, 451, 1276
PspEI GGTNACC 1 cut(s) 920
PspGI CCWGG 3 cut(s) 232, 451, 1276
PspN4I GGNNCC 5 cut(s) 900, 1282, 1314, 1363, 1417
PspPI GGNCC 5 cut(s) 505, 678, 839, 1361, 1415
PspXI VCTCGAGB 1 cut(s) 627
PsuI RGATCY 2 cut(s) 989, 1084
RseI CAYNNNNRTG 2 cut(s) 686, 872
SaqAI TTAA 5 cut(s) 90, 345, 768, 1113, 1440
SatI GCNGC 5 cut(s) 354, 708, 897, 1143, 1217
Sau3AI GATC 6 cut(s) 420, 989, 1084, 1203, 1228, 1454
Sau96I GGNCC 5 cut(s) 505, 678, 839, 1361, 1415
SchI GAGTC 2 cut(s) 15, 475
ScrFI CCNGG 3 cut(s) 234, 453, 1278
SduI GDGCHC 4 cut(s) 828, 884, 1023, 1042
SexAI ACCWGGT 1 cut(s) 451
SfaNI GCATC 3 cut(s) 116, 594, 781
SfcI CTRYAG 1 cut(s) 1470
Sfr274I CTCGAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 4 cut(s) 678, 839, 1361, 1415
SlaI CTCGAG 1 cut(s) 627
SmiMI CAYNNNNRTG 2 cut(s) 686, 872
SmlI CTYRAG 3 cut(s) 344, 627, 1223
SmoI CTYRAG 3 cut(s) 344, 627, 1223
SpeI ACTAGT 1 cut(s) 254
SphI GCATGC 1 cut(s) 1264
Sse9I AATT 7 cut(s) 87, 154, 361, 545, 569, 971, 1110
SsiI CCGC 4 cut(s) 393, 794, 896, 1399
SspMI CTAG 4 cut(s) 171, 255, 1173, 1338
StyD4I CCNGG 3 cut(s) 232, 451, 1276
StyI CCWWGG 3 cut(s) 674, 1012, 1255
TaaI ACNGT 2 cut(s) 890, 1151
TaiI ACGT 1 cut(s) 440
TaqI TCGA 6 cut(s) 322, 588, 628, 760, 1006, 1090
TasI AATT 7 cut(s) 87, 154, 361, 545, 569, 971, 1110
TauI GCSGC 1 cut(s) 899
TfiI GAWTC 4 cut(s) 327, 376, 643, 737
Tru1I TTAA 5 cut(s) 90, 345, 768, 1113, 1440
Tru9I TTAA 5 cut(s) 90, 345, 768, 1113, 1440
TscAI CASTG 3 cut(s) 169, 430, 1154
TseFI GTSAC 1 cut(s) 920
TseI GCWGC 4 cut(s) 353, 707, 1142, 1216
Tsp45I GTSAC 1 cut(s) 920
TspDTI ATGAA 6 cut(s) 99, 106, 119, 198, 704, 719
TspRI CASTG 3 cut(s) 169, 430, 1154
Van91I CCANNNNNTGG 1 cut(s) 1052
Vha464I CTTAAG 1 cut(s) 344
VpaK11BI GGWCC 4 cut(s) 678, 839, 1361, 1415
XapI RAATTY 1 cut(s) 361
XbaI TCTAGA 1 cut(s) 1337
XceI RCATGY 1 cut(s) 1264
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 627
XmiI GTMKAC 2 cut(s) 197, 408
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 4 cut(s) 171, 255, 1173, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.