Rw2G050530
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000641
Physical Location & Seq
Reverse (-)
78109392 .. 78112973
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050530.1

Sequence Viewer

Length: 1425 bp
ATGGGTTTGTTAAAGGTGGAGACTTGGGATGGGAGCAAATCAAAGGGAAGGAAGAAGAAGAAGAAGCATGAGGCTGTGGAGGAAACTGGGTGTTGCATTAAGTTCTCTTTTGGGATTTGCATGCCTTCTTCAAGATCCAAAGTTGATAGCACCATGACTGGGACTACTATATCTTTTGCGGAAACTAAATCTTCAAATGAAAAAAGTAGAGATCGAGACCTTACTCGTGGAGGGTCCTCTACAACTACTAGTAATGCTGGAAGTACTACATCCACACCCTATTTCAGTGAGGAACTGAAGGTTGCTTCTCAGCTGCGGAAATTCACATTTAATGACCTTAAGTTAGCAACTAGAAACTTCAGACCGGAGTCTCTTCTTGGGGAGGGTGGATTTGGTTGTGTGTTCAAGGGCTGGATAGAGGAGAATGGAACTGCTCCTGTGAAGCCTGGTACTGGGCTTACAGTCGCAGTTAAGACCCTCAACCATGATGGACTTCAGGGTCACAAAGAATGGCTTGCTGAAATTTATTTTCTTGGTGATCTCATCCATCCAAATTTGGTAAAATTGATTGGTTACTCCATTGAAGATGATCAGAGGTTGCTGGTTTATGAGTTTTTACCGCGAGGAAGTTTGGAAAACCACCTATTCAGAAGGTCCCTGCCTCTTCCTTGGTCCATTAGAATGAAAATTGCACTTGGTGCTGCAAAGGGTCTTGCCTTTCTTCATGAAGAAGTTCAAAAACCTGTCATATACCGTGACTTCAAAACATCTAATATCCTATTAGACGCGGATTACAACGCCAAGCTCTCGGATTTTGGACTTGCCAAAGATGGTCCTGAAGGTGATAAGACTCATGTGTCTACGCGAGTCATGGGAACCTATGGTTATGCAGCCCCAGAATATGTGATGACTGGACATTTGACATCAAAGAGTGATGTCTACAGTTTTGGAGTAGTCCTACTTGAAATGCTAAATGGAAGAAGATCCATGGATAAAAATAGACCAAATGGGGAACACAATCTTGTGGAATGGGCGAGACAACTTTTTGGAGACAAGAGGAGGTTATTCCAGTTAATTGACCCCCGGCTCGAAGGCCATTTTTCAATCAAGGGTGCACAAAAAGCTATCCAACTGGCTGCCCGCTGCCTCAGCCGTGACCCAAAAGCCAGACCAATGATGAGTGAAGTTGTTGAAGCCCTAAAGCCTTTACCAAGCCTCAAGGACATGGCCAGTTCTTCTTATCACTTTCAAACCATGCAAGCAGCTCGTACCAGAGCCAACTTGAATGCTAAGAATGGCATTAGAACACAAGCAATCTTTGTACCGAGGAATGGACAACCGGTAAGGAGCTTGTCAAGCCCAAAGGGTCCTCCCGCTTCTCCATGTTATCCTCAGAAATCACCTAAACCTAACTCGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

474

Amino Acids

52.63

Weight (kDa)

9.61

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 123 - 398 1.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 124 - 400 6e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 498, 856
AccI GTMKAC 2 cut(s) 860, 939
AccII CGCG 3 cut(s) 622, 788, 865
AciI CCGC 6 cut(s) 179, 316, 620, 788, 1141, 1374
AclWI GGATC 2 cut(s) 129, 978
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 3 cut(s) 320, 522, 553
AcuI CTGAAG 4 cut(s) 317, 343, 479, 858
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 4 cut(s) 265, 451, 1270, 1323
AfiI CCNNNNNNNGG 3 cut(s) 159, 452, 1331
AflII CTTAAG 1 cut(s) 338
AgeI ACCGGT 1 cut(s) 1339
AhlI ACTAGT 1 cut(s) 248
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 5 cut(s) 313, 805, 1124, 1265, 1350
AluI AGCT 5 cut(s) 313, 805, 1124, 1265, 1350
Alw21I GWGCWC 1 cut(s) 1117
Alw26I GTCTC 5 cut(s) 14, 210, 375, 1030, 1044
Alw44I GTGCAC 1 cut(s) 1113
AlwI GGATC 2 cut(s) 129, 978
AoxI GGCC 2 cut(s) 1093, 1227
ApaLI GTGCAC 1 cut(s) 1113
ApeKI GCWGC 6 cut(s) 313, 701, 890, 1136, 1143, 1262
ApoI RAATTY 3 cut(s) 320, 522, 553
ArsI GACNNNNNNTTYG 2 cut(s) 901, 933
AsiGI ACCGGT 1 cut(s) 1339
Asp700I GAANNNNTTC 1 cut(s) 732
AspS9I GGNCC 5 cut(s) 234, 654, 672, 833, 1367
AsuC2I CCSGG 1 cut(s) 1084
AsuHPI GGTGA 3 cut(s) 548, 854, 1392
AvaII GGWCC 5 cut(s) 234, 654, 672, 833, 1367
BaeGI GKGCMC 1 cut(s) 1117
BalI TGGCCA 1 cut(s) 1229
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 1117
BbvCI CCTCAGC 1 cut(s) 1148
BbvI GCAGC 6 cut(s) 300, 688, 902, 1123, 1130, 1274
BccI CCATC 4 cut(s) 23, 482, 555, 824
BceAI ACGGC 1 cut(s) 1137
BcgI CGANNNNNNTGC 2 cut(s) 1247, 1281
BciT130I CCWGG 1 cut(s) 447
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 1 cut(s) 1084
BcoDI GTCTC 5 cut(s) 14, 210, 375, 1030, 1044
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 2 cut(s) 249, 351
BfmI CTRYAG 1 cut(s) 940
BfrI CTTAAG 1 cut(s) 338
BisI GCNGC 6 cut(s) 314, 702, 891, 1137, 1144, 1263
BlsI GCNGC 6 cut(s) 315, 703, 892, 1138, 1145, 1264
BmcAI AGTACT 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 447, 1084
Bme18I GGWCC 5 cut(s) 234, 654, 672, 833, 1367
BmgT120I GGNCC 5 cut(s) 234, 654, 672, 833, 1367
BmiI GGNNCC 4 cut(s) 235, 656, 877, 1368
BmrFI CCNGG 2 cut(s) 447, 1084
BmrI ACTGGG 3 cut(s) 96, 168, 462
BmuI ACTGGG 3 cut(s) 96, 168, 462
BoxI GACNNNNGTC 1 cut(s) 367
Bpu10I CCTNAGC 1 cut(s) 1148
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 1084
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 4 cut(s) 668, 987, 1082, 1325
BsaWI WCCGGW 2 cut(s) 364, 1339
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 452, 1331
Bse118I RCCGGY 1 cut(s) 1339
Bse1I ACTGG 7 cut(s) 91, 163, 457, 916, 1069, 1137, 1230
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 4 cut(s) 668, 987, 1082, 1325
BseGI GGATG 4 cut(s) 34, 269, 543, 547
BseLI CCNNNNNNNGG 3 cut(s) 159, 452, 1331
BseMII CTCAG 3 cut(s) 323, 1162, 1406
BseNI ACTGG 7 cut(s) 91, 163, 457, 916, 1069, 1137, 1230
BseRI GAGGAG 2 cut(s) 434, 1072
BseSI GKGCMC 1 cut(s) 1117
BseXI GCAGC 6 cut(s) 300, 688, 902, 1123, 1130, 1274
Bsh1236I CGCG 3 cut(s) 622, 788, 865
BshFI GGCC 2 cut(s) 1095, 1229
BshTI ACCGGT 1 cut(s) 1339
BsiHKAI GWGCWC 1 cut(s) 1117
BsiSI CCGG 3 cut(s) 365, 1084, 1340
BslFI GGGAC 2 cut(s) 175, 640
BslI CCNNNNNNNGG 3 cut(s) 159, 452, 1331
BsmAI GTCTC 5 cut(s) 14, 210, 375, 1030, 1044
BsmFI GGGAC 2 cut(s) 175, 640
BsmI GAATGC 1 cut(s) 1291
BsnI GGCC 2 cut(s) 1095, 1229
Bso31I GGTCTC 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 1117
Bsp143I GATC 5 cut(s) 134, 211, 538, 589, 983
Bsp19I CCATGG 1 cut(s) 987
BspACI CCGC 6 cut(s) 179, 316, 620, 788, 1141, 1374
BspANI GGCC 2 cut(s) 1095, 1229
BspCNI CTCAG 3 cut(s) 322, 1161, 1405
BspFNI CGCG 3 cut(s) 622, 788, 865
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 4 cut(s) 235, 656, 877, 1368
BspPI GGATC 2 cut(s) 129, 978
BspTI CTTAAG 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 1339
BsrI ACTGG 7 cut(s) 91, 163, 457, 916, 1069, 1137, 1230
BssAI RCCGGY 1 cut(s) 1339
BssECI CCNNGG 4 cut(s) 668, 987, 1082, 1325
BssMI GATC 5 cut(s) 134, 211, 538, 589, 983
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 668, 987
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 463, 755, 944
Bst6I CTCTTC 2 cut(s) 378, 669
BstAFI CTTAAG 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 698
BstC8I GCNNGC 4 cut(s) 122, 516, 1141, 1260
BstDEI CTNAG 4 cut(s) 309, 1148, 1290, 1392
BstDSI CCRYGG 1 cut(s) 987
BstF5I GGATG 4 cut(s) 34, 269, 543, 547
BstFNI CGCG 3 cut(s) 622, 788, 865
BstKTI GATC 5 cut(s) 137, 214, 541, 592, 986
BstMAI GTCTC 5 cut(s) 14, 210, 375, 1030, 1044
BstMBI GATC 5 cut(s) 134, 211, 538, 589, 983
BstMWI GCNNNNNNNGC 4 cut(s) 698, 1121, 1149, 1356
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 1 cut(s) 124
BstPAI GACNNNNGTC 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 445, 1082
BstSFI CTRYAG 1 cut(s) 940
BstSLI GKGCMC 1 cut(s) 1117
BstUI CGCG 3 cut(s) 622, 788, 865
BstV1I GCAGC 6 cut(s) 300, 688, 902, 1123, 1130, 1274
BstX2I RGATCY 2 cut(s) 134, 983
BstYI RGATCY 2 cut(s) 134, 983
BsuRI GGCC 2 cut(s) 1095, 1229
BtgI CCRYGG 1 cut(s) 987
BtsCI GGATG 4 cut(s) 34, 269, 543, 547
BtsIMutI CAGTG 1 cut(s) 292
Cac8I GCNNGC 4 cut(s) 122, 516, 1141, 1260
CciI TCATGA 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 1339
Cfr13I GGNCC 5 cut(s) 234, 654, 672, 833, 1367
CseI GACGC 1 cut(s) 794
Csp6I GTAC 4 cut(s) 264, 450, 1269, 1322
CspAI ACCGGT 1 cut(s) 1339
CviQI GTAC 4 cut(s) 264, 450, 1269, 1322
DdeI CTNAG 4 cut(s) 309, 1148, 1290, 1392
DpnI GATC 5 cut(s) 136, 213, 540, 591, 985
DpnII GATC 5 cut(s) 134, 211, 538, 589, 983
DraIII CACNNNGTG 1 cut(s) 698
DrdI GACNNNNNNGTC 2 cut(s) 498, 856
DseDI GACNNNNNNGTC 2 cut(s) 498, 856
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 2 cut(s) 378, 669
EarI CTCTTC 2 cut(s) 378, 669
Eco130I CCWWGG 2 cut(s) 668, 987
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 5 cut(s) 234, 654, 672, 833, 1367
Eco57I CTGAAG 4 cut(s) 317, 343, 479, 858
EcoO109I RGGNCCY 3 cut(s) 234, 654, 1367
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 2 cut(s) 668, 987
ErhI CCWWGG 2 cut(s) 668, 987
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 2 cut(s) 175, 640
FauI CCCGC 2 cut(s) 1148, 1381
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 2 cut(s) 860, 939
Fnu4HI GCNGC 6 cut(s) 314, 702, 891, 1137, 1144, 1263
FokI GGATG 4 cut(s) 41, 256, 530, 534
Fsp4HI GCNGC 6 cut(s) 314, 702, 891, 1137, 1144, 1263
FspBI CTAG 2 cut(s) 249, 351
GluI GCNGC 6 cut(s) 314, 702, 891, 1137, 1144, 1263
HaeIII GGCC 2 cut(s) 1095, 1229
HapII CCGG 3 cut(s) 365, 1084, 1340
HgaI GACGC 1 cut(s) 794
HinfI GANTC 3 cut(s) 368, 850, 867
HpaII CCGG 3 cut(s) 365, 1084, 1340
HphI GGTGA 3 cut(s) 548, 854, 1392
Hpy166II GTNNAC 3 cut(s) 861, 940, 1115
Hpy188I TCNGA 5 cut(s) 362, 594, 650, 811, 1395
Hpy188III TCNNGA 4 cut(s) 132, 215, 725, 836
Hpy8I GTNNAC 3 cut(s) 861, 940, 1115
HpyAV CCTTC 6 cut(s) 42, 135, 292, 645, 833, 1085
HpyCH4III ACNGT 3 cut(s) 463, 755, 944
HpyCH4V TGCA 7 cut(s) 96, 120, 692, 704, 890, 1115, 1258
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 1121, 1149, 1356
HpyF3I CTNAG 4 cut(s) 309, 1148, 1290, 1392
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 5 cut(s) 134, 211, 538, 589, 983
LmnI GCTCC 3 cut(s) 33, 439, 1347
Lsp1109I GCAGC 6 cut(s) 300, 688, 902, 1123, 1130, 1274
MaeI CTAG 2 cut(s) 249, 351
MaeIII GTNAC 4 cut(s) 500, 572, 755, 1154
MalI GATC 5 cut(s) 136, 213, 540, 591, 985
MboI GATC 5 cut(s) 134, 211, 538, 589, 983
MflI RGATCY 2 cut(s) 134, 983
MhlI GDGCHC 1 cut(s) 1117
MlsI TGGCCA 1 cut(s) 1229
MluCI AATT 6 cut(s) 320, 522, 553, 563, 687, 1074
MluNI TGGCCA 1 cut(s) 1229
MlyI GAGTC 3 cut(s) 377, 844, 876
MmeI TCCRAC 1 cut(s) 1153
Mox20I TGGCCA 1 cut(s) 1229
MroXI GAANNNNTTC 1 cut(s) 732
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 6 cut(s) 11, 99, 330, 339, 471, 1073
MslI CAYNNNNRTG 1 cut(s) 680
Msp20I TGGCCA 1 cut(s) 1229
MspA1I CMGCKG 2 cut(s) 313, 1143
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 3 cut(s) 365, 1084, 1340
MspR9I CCNGG 2 cut(s) 447, 1084
Mva1269I GAATGC 1 cut(s) 1291
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 3 cut(s) 622, 788, 865
MwoI GCNNNNNNNGC 4 cut(s) 698, 1121, 1149, 1356
NciI CCSGG 1 cut(s) 1084
NcoI CCATGG 1 cut(s) 987
NdeII GATC 5 cut(s) 134, 211, 538, 589, 983
NlaIV GGNNCC 4 cut(s) 235, 656, 877, 1368
NmuCI GTSAC 3 cut(s) 500, 755, 1154
NspI RCATGY 1 cut(s) 124
PaeI GCATGC 1 cut(s) 124
PagI TCATGA 1 cut(s) 724
PctI GAATGC 1 cut(s) 1291
PdmI GAANNNNTTC 1 cut(s) 732
PinAI ACCGGT 1 cut(s) 1339
PkrI GCNGC 6 cut(s) 315, 703, 892, 1138, 1145, 1264
PleI GAGTC 3 cut(s) 376, 844, 875
PpsI GAGTC 3 cut(s) 376, 844, 875
PpuMI RGGWCCY 3 cut(s) 234, 654, 1367
PshAI GACNNNNGTC 1 cut(s) 367
Psp5II RGGWCCY 3 cut(s) 234, 654, 1367
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 4 cut(s) 235, 656, 877, 1368
PspPI GGNCC 5 cut(s) 234, 654, 672, 833, 1367
PspPPI RGGWCCY 3 cut(s) 234, 654, 1367
PsuI RGATCY 2 cut(s) 134, 983
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 4 cut(s) 265, 451, 1270, 1323
RsaNI GTAC 4 cut(s) 264, 450, 1269, 1322
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 6 cut(s) 11, 99, 330, 339, 471, 1073
SatI GCNGC 6 cut(s) 314, 702, 891, 1137, 1144, 1263
Sau3AI GATC 5 cut(s) 134, 211, 538, 589, 983
Sau96I GGNCC 5 cut(s) 234, 654, 672, 833, 1367
ScaI AGTACT 1 cut(s) 265
SchI GAGTC 3 cut(s) 377, 844, 876
ScrFI CCNGG 2 cut(s) 447, 1084
SduI GDGCHC 1 cut(s) 1117
SfcI CTRYAG 1 cut(s) 940
SinI GGWCC 5 cut(s) 234, 654, 672, 833, 1367
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 2 cut(s) 338, 1217
SmoI CTYRAG 2 cut(s) 338, 1217
SpeI ACTAGT 1 cut(s) 248
SphI GCATGC 1 cut(s) 124
Sse9I AATT 6 cut(s) 320, 522, 553, 563, 687, 1074
SsiI CCGC 6 cut(s) 179, 316, 620, 788, 1141, 1374
SspMI CTAG 2 cut(s) 249, 351
StyD4I CCNGG 2 cut(s) 445, 1082
StyI CCWWGG 2 cut(s) 668, 987
TaaI ACNGT 3 cut(s) 463, 755, 944
TaqI TCGA 3 cut(s) 214, 1089, 1415
TasI AATT 6 cut(s) 320, 522, 553, 563, 687, 1074
TatI WGTACW 1 cut(s) 263
Tru1I TTAA 6 cut(s) 11, 99, 330, 339, 471, 1073
Tru9I TTAA 6 cut(s) 11, 99, 330, 339, 471, 1073
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 3 cut(s) 500, 755, 1154
TseI GCWGC 6 cut(s) 313, 701, 890, 1136, 1143, 1262
Tsp45I GTSAC 3 cut(s) 500, 755, 1154
TspDTI ATGAA 4 cut(s) 213, 698, 713, 741
TspRI CASTG 1 cut(s) 292
Vha464I CTTAAG 1 cut(s) 338
VneI GTGCAC 1 cut(s) 1113
VpaK11BI GGWCC 5 cut(s) 234, 654, 672, 833, 1367
XapI RAATTY 3 cut(s) 320, 522, 553
XceI RCATGY 1 cut(s) 124
XmiI GTMKAC 2 cut(s) 860, 939
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 2 cut(s) 249, 351
ZrmI AGTACT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.