Prupe.3G270000_v2.0.a1

receptor-like protein kinase At5g15080

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000641
Physical Location & Seq
Forward (+)
25150881 .. 25156685
5805 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G270000.1

Sequence Viewer

Length: 1437 bp
ATGGGATTAGGCCCTGATGCTTTTCATGCTGGAGCTTGGAATGTGGGAAAATCAAAGGGAAAGAAAAAGAAAGATGATGAAGATGAAATGACAGGGTGTTGGATGAAGTTTAGGCTCATGGGAGGATGCGTGTCTTCAAGGACCAAAGTGGATAGCTCTACAAGTGGAACTAGCACACAAGATGCGGAAAGTAAATGTACAAATGACTCCATCAAAGACCAGCTAGCTGCTCCAGTTGCTTCGTCTTCAACCACTAGCAACACAGAAAGCACACTGTCCACTCCCAAGGCAGCTGAGGAGCTGAAAGTTGCTTCTCAGCTTCGAAAATTCACCTTTGATGAGCTTAAGTCAGTGACAAAGAATTTTAGACCTGTGAATCTTTTGGGCGAGGGTGGATTTGGTTGTGTATTCAAAGGCTGGATCGATGAAAATGGAACTGCTCCAGTAAAACCTGGAACTGGGCTTGCGGTTGCTGTAAAGACTCTGAACCATGATGGACTTCAAGGTCATAAAGAATGGCTGGCTGAAGTTATTTATCTTGGTGACCTACTTCATCCTAATTTAGTCAAACTATATGGCTATTGCATTGAGGATGATCAAAGATTACTTGTGTATGAGTTTATGCCTCGGGGAAGCTTGGAGAATCACCTCTTTAGAAGGTCCTTGCCGCTTCCTTGGTCTATCCGAATGAAAATAGCTCTTGGTGCTGCAAAGGGTCTTGCTTTTCTTCACGAGGAAGCTGAAAGGCCAGTGATTTATCGAGATTTTAAAACATCTAATATATTGTTGGATGCGGATTACAACGCCAAGCTTTCTGATTTTGGCCTCGCTAAAGATGCTCCAGAGGGAGATAAAACTCATGTATCCACCCGAGTGATGGGCACTTATGGTTATGCAGCCCCTGAGTATGTTATGACAGGACATCTTACTTCAAAAAGTGATGTATACAGCTTCGGGGTGGTTCTGCTCGAAATGCTGGTTGGTCGAAGATCAATGGACAAAAACCGACCCAATGGAGAGGTTAACTTGGTTGAGTGGGCACGCCCACACCTTGGAGATAGACGAAGATTCTATCGACTAGTAGATCCTCGACTTGAAGGCCGCTTCTCAATCAAAGGTGCCCAGAAGGCACTTCAGTTGGCTGCCCGTTGTCTTAGTCGTGATCCCAAGGCTAGACCTCAGATGAGTGAAGTTGTTGAAGTGCTGCAGCCTCTACCCACTCTCAAGGATATGGCATCTTCCTCATCTTACTTCCAAGCCTTGCAAATAGAGCGGCCTGGCTCCAATCTAAATTCTCGGAATGGCAGTAGAGGGCAGGCTGGGGTTTCAAGGAATGGACAACCAACTAGAAGCCGGTCCATGCCAAATGGTCCGCATGCCTCACCATATCACCTTAACCATCCTCACCGATCACCCAAACCTGTCGCTCAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

479

Amino Acids

52.7

Weight (kDa)

9.32

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 1118
AccB7I CCANNNNNTGG 1 cut(s) 1052
AccBSI CCGCTC 1 cut(s) 1273
AccI GTMKAC 1 cut(s) 945
AciI CCGC 7 cut(s) 185, 467, 668, 794, 1102, 1273, 1373
AclWI GGATC 3 cut(s) 428, 1079, 1157
AcsI RAATTY 3 cut(s) 326, 361, 1291
AcuI CTGAAG 2 cut(s) 546, 1118
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 3 cut(s) 458, 877, 1052
AflII CTTAAG 1 cut(s) 344
AgsI TTSAA 8 cut(s) 138, 249, 412, 503, 933, 1097, 1199, 1329
AhlI ACTAGT 1 cut(s) 1078
AjnI CCWGG 2 cut(s) 451, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 963, 995
AleI CACNNNNGTG 1 cut(s) 872
AlwI GGATC 3 cut(s) 428, 1079, 1157
AlwNI CAGNNNCTG 1 cut(s) 902
Ama87I CYCGRG 2 cut(s) 627, 870
AoxI GGCC 5 cut(s) 10, 746, 823, 1099, 1274
ApeKI GCWGC 7 cut(s) 227, 290, 707, 896, 1142, 1204, 1207
ApoI RAATTY 3 cut(s) 326, 361, 1291
AspS9I GGNCC 5 cut(s) 11, 141, 660, 1356, 1370
AsuHPI GGTGA 7 cut(s) 322, 554, 638, 1374, 1382, 1397, 1404
AsuII TTCGAA 1 cut(s) 322
AsuNHI GCTAGC 1 cut(s) 223
AvaI CYCGRG 2 cut(s) 627, 870
AvaII GGWCC 4 cut(s) 141, 660, 1356, 1370
BaeGI GKGCMC 3 cut(s) 884, 1042, 1123
BaeI ACNNNNGTAYC 2 cut(s) 846, 879
BanI GGYRCC 1 cut(s) 1118
BauI CACGAG 1 cut(s) 731
BbsI GAAGAC 2 cut(s) 126, 237
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 7 cut(s) 214, 302, 694, 908, 1129, 1191, 1219
BccI CCATC 4 cut(s) 218, 488, 871, 1407
BciT130I CCWGG 2 cut(s) 453, 1278
BciVI GTATCC 1 cut(s) 874
BclI TGATCA 1 cut(s) 595
BcuI ACTAGT 1 cut(s) 1078
BfaI CTAG 6 cut(s) 171, 224, 255, 1079, 1173, 1347
BfmI CTRYAG 1 cut(s) 1205
BfrI CTTAAG 1 cut(s) 344
BfuI GTATCC 1 cut(s) 874
BglI GCCNNNNNGGC 1 cut(s) 1127
Bme1390I CCNGG 2 cut(s) 453, 1278
Bme18I GGWCC 4 cut(s) 141, 660, 1356, 1370
BmeT110I CYCGRG 2 cut(s) 627, 870
BmgT120I GGNCC 5 cut(s) 11, 141, 660, 1356, 1370
BmiI GGNNCC 2 cut(s) 1120, 1282
BmrFI CCNGG 2 cut(s) 453, 1278
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 6 cut(s) 7, 116, 172, 781, 826, 1244
BmtI GCTAGC 1 cut(s) 227
BmuI ACTGGG 1 cut(s) 468
BpiI GAAGAC 2 cut(s) 126, 237
BpmI CTGGAG 4 cut(s) 51, 216, 426, 825
Bpu10I CCTNAGC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 1208
Bsa29I ATCGAT 1 cut(s) 423
BsaJI CCNNGG 5 cut(s) 285, 626, 674, 1051, 1167
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 3 cut(s) 458, 877, 1052
Bse118I RCCGGY 1 cut(s) 1353
Bse1I ACTGG 4 cut(s) 233, 443, 463, 749
BseBI CCWGG 2 cut(s) 453, 1278
BseCI ATCGAT 1 cut(s) 423
BseDI CCNNGG 5 cut(s) 285, 626, 674, 1051, 1167
BseGI GGATG 6 cut(s) 108, 131, 553, 598, 796, 1399
BseLI CCNNNNNNNGG 3 cut(s) 458, 877, 1052
BseMII CTCAG 4 cut(s) 285, 329, 894, 1193
BseNI ACTGG 4 cut(s) 233, 443, 463, 749
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 3 cut(s) 884, 1042, 1123
BseXI GCAGC 7 cut(s) 214, 302, 694, 908, 1129, 1191, 1219
BseYI CCCAGC 1 cut(s) 1319
BshFI GGCC 5 cut(s) 12, 748, 825, 1101, 1276
BshNI GGYRCC 1 cut(s) 1118
BshVI ATCGAT 1 cut(s) 423
BsiHKCI CYCGRG 2 cut(s) 627, 870
BsiSI CCGG 1 cut(s) 1354
BslI CCNNNNNNNGG 3 cut(s) 458, 877, 1052
BsnI GGCC 5 cut(s) 12, 748, 825, 1101, 1276
BsoBI CYCGRG 2 cut(s) 627, 870
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 3 cut(s) 884, 1042, 1123
Bsp1407I TGTACA 1 cut(s) 197
Bsp143I GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
BspACI CCGC 7 cut(s) 185, 467, 668, 794, 1102, 1273, 1373
BspANI GGCC 5 cut(s) 12, 748, 825, 1101, 1276
BspCNI CTCAG 4 cut(s) 286, 328, 895, 1192
BspDI ATCGAT 1 cut(s) 423
BspLI GGNNCC 2 cut(s) 1120, 1282
BspMAI CTGCAG 1 cut(s) 1209
BspOI GCTAGC 1 cut(s) 227
BspPI GGATC 3 cut(s) 428, 1079, 1157
BspT104I TTCGAA 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 1118
BspTI CTTAAG 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 1273
BsrFI RCCGGY 1 cut(s) 1353
BsrGI TGTACA 1 cut(s) 197
BsrI ACTGG 4 cut(s) 233, 443, 463, 749
BssAI RCCGGY 1 cut(s) 1353
BssECI CCNNGG 5 cut(s) 285, 626, 674, 1051, 1167
BssMI GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
BssNAI GTATAC 1 cut(s) 946
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 4 cut(s) 285, 674, 1051, 1167
Bst1107I GTATAC 1 cut(s) 946
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 2 cut(s) 453, 1278
Bst4CI ACNGT 1 cut(s) 276
BstAFI CTTAAG 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 6 cut(s) 225, 465, 522, 1042, 1317, 1377
BstDEI CTNAG 5 cut(s) 294, 315, 903, 1154, 1179
BstEII GGTNACC 1 cut(s) 542
BstF5I GGATG 6 cut(s) 108, 131, 553, 598, 796, 1399
BstKTI GATC 6 cut(s) 423, 598, 992, 1087, 1165, 1412
BstMBI GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
BstMWI GCNNNNNNNGC 7 cut(s) 26, 236, 704, 836, 973, 1127, 1270
BstNI CCWGG 2 cut(s) 453, 1278
BstNSI RCATGY 1 cut(s) 1379
BstPI GGTNACC 1 cut(s) 542
BstSCI CCNGG 2 cut(s) 451, 1276
BstSFI CTRYAG 1 cut(s) 1205
BstSLI GKGCMC 3 cut(s) 884, 1042, 1123
BstV1I GCAGC 7 cut(s) 214, 302, 694, 908, 1129, 1191, 1219
BstV2I GAAGAC 2 cut(s) 126, 237
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BstZ17I GTATAC 1 cut(s) 946
Bsu15I ATCGAT 1 cut(s) 423
BsuI GTATCC 1 cut(s) 874
BsuRI GGCC 5 cut(s) 12, 748, 825, 1101, 1276
BsuTUI ATCGAT 1 cut(s) 423
BtsCI GGATG 6 cut(s) 108, 131, 553, 598, 796, 1399
BtsIMutI CAGTG 3 cut(s) 272, 357, 756
Cac8I GCNNGC 6 cut(s) 225, 465, 522, 1042, 1317, 1377
CaiI CAGNNNCTG 1 cut(s) 902
Cfr10I RCCGGY 1 cut(s) 1353
Cfr13I GGNCC 5 cut(s) 11, 141, 660, 1356, 1370
ClaI ATCGAT 1 cut(s) 423
Csp6I GTAC 1 cut(s) 198
CviAII CATG 6 cut(s) 26, 118, 491, 860, 1360, 1376
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 5 cut(s) 294, 315, 903, 1154, 1179
DpnI GATC 6 cut(s) 422, 597, 991, 1086, 1164, 1411
DpnII GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
DraI TTTAAA 1 cut(s) 769
DrdI GACNNNNNNGTC 1 cut(s) 504
DseDI GACNNNNNNGTC 1 cut(s) 504
Eco130I CCWWGG 4 cut(s) 285, 674, 1051, 1167
Eco47I GGWCC 4 cut(s) 141, 660, 1356, 1370
Eco57I CTGAAG 2 cut(s) 546, 1118
Eco88I CYCGRG 2 cut(s) 627, 870
Eco91I GGTNACC 1 cut(s) 542
EcoO109I RGGNCCY 2 cut(s) 11, 660
EcoO65I GGTNACC 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 451, 1276
EcoT14I CCWWGG 4 cut(s) 285, 674, 1051, 1167
ErhI CCWWGG 4 cut(s) 285, 674, 1051, 1167
FaeI CATG 6 cut(s) 29, 121, 494, 863, 1363, 1379
FatI CATG 6 cut(s) 25, 117, 490, 859, 1359, 1375
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 945
FokI GGATG 6 cut(s) 115, 138, 540, 605, 803, 1386
FspBI CTAG 6 cut(s) 171, 224, 255, 1079, 1173, 1347
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 4 cut(s) 51, 216, 426, 825
HaeIII GGCC 5 cut(s) 12, 748, 825, 1101, 1276
HapII CCGG 1 cut(s) 1354
Hin1II CATG 6 cut(s) 29, 121, 494, 863, 1363, 1379
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 2 cut(s) 634, 809
HinfI GANTC 5 cut(s) 206, 376, 481, 643, 1068
HpaI GTTAAC 1 cut(s) 1024
HpaII CCGG 1 cut(s) 1354
HphI GGTGA 7 cut(s) 322, 554, 638, 1374, 1382, 1397, 1404
Hpy166II GTNNAC 3 cut(s) 279, 946, 1024
Hpy188I TCNGA 5 cut(s) 486, 686, 817, 1182, 1299
Hpy188III TCNNGA 4 cut(s) 731, 761, 842, 1160
Hpy8I GTNNAC 3 cut(s) 279, 946, 1024
HpyAV CCTTC 3 cut(s) 651, 1091, 1120
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 585, 710, 896, 1207, 1264
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 236, 704, 836, 973, 1127, 1270
HpyF3I CTNAG 5 cut(s) 294, 315, 903, 1154, 1179
Hsp92II CATG 6 cut(s) 29, 121, 494, 863, 1363, 1379
Ksp22I TGATCA 1 cut(s) 595
KspAI GTTAAC 1 cut(s) 1024
Kzo9I GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
LmnI GCTCC 6 cut(s) 32, 235, 298, 445, 844, 1286
Lsp1109I GCAGC 7 cut(s) 214, 302, 694, 908, 1129, 1191, 1219
LweI GCATC 6 cut(s) 7, 116, 172, 781, 826, 1244
MaeI CTAG 6 cut(s) 171, 224, 255, 1079, 1173, 1347
MaeIII GTNAC 2 cut(s) 352, 542
MalI GATC 6 cut(s) 422, 597, 991, 1086, 1164, 1411
MbiI CCGCTC 1 cut(s) 1273
MboI GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
MboII GAAGA 7 cut(s) 92, 126, 237, 719, 999, 1077, 1230
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 3 cut(s) 884, 1042, 1123
MluCI AATT 4 cut(s) 326, 361, 559, 1291
MlyI GAGTC 2 cut(s) 200, 475
MmeI TCCRAC 2 cut(s) 80, 768
MseI TTAA 4 cut(s) 345, 768, 1023, 1395
MslI CAYNNNNRTG 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 1 cut(s) 1354
MspR9I CCNGG 2 cut(s) 453, 1278
MvaI CCWGG 2 cut(s) 453, 1278
MwoI GCNNNNNNNGC 7 cut(s) 26, 236, 704, 836, 973, 1127, 1270
NdeII GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
NheI GCTAGC 1 cut(s) 223
NlaIII CATG 6 cut(s) 29, 121, 494, 863, 1363, 1379
NlaIV GGNNCC 2 cut(s) 1120, 1282
NmuCI GTSAC 2 cut(s) 352, 542
NspI RCATGY 1 cut(s) 1379
NspV TTCGAA 1 cut(s) 322
OliI CACNNNNGTG 1 cut(s) 872
PaeI GCATGC 1 cut(s) 1379
PfeI GAWTC 3 cut(s) 376, 643, 1068
PflMI CCANNNNNTGG 1 cut(s) 1052
PleI GAGTC 2 cut(s) 200, 475
PpsI GAGTC 2 cut(s) 200, 475
PpuMI RGGWCCY 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 451, 1276
PspEI GGTNACC 1 cut(s) 542
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 2 cut(s) 451, 1276
PspN4I GGNNCC 2 cut(s) 1120, 1282
PspPI GGNCC 5 cut(s) 11, 141, 660, 1356, 1370
PspPPI RGGWCCY 1 cut(s) 660
PstI CTGCAG 1 cut(s) 1209
PstNI CAGNNNCTG 1 cut(s) 902
PsuI RGATCY 1 cut(s) 1084
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 1 cut(s) 872
SaqAI TTAA 4 cut(s) 345, 768, 1023, 1395
Sau3AI GATC 6 cut(s) 420, 595, 989, 1084, 1162, 1409
Sau96I GGNCC 5 cut(s) 11, 141, 660, 1356, 1370
SchI GAGTC 2 cut(s) 200, 475
ScrFI CCNGG 2 cut(s) 453, 1278
SduI GDGCHC 3 cut(s) 884, 1042, 1123
SfaNI GCATC 6 cut(s) 7, 116, 172, 781, 826, 1244
SfcI CTRYAG 1 cut(s) 1205
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 4 cut(s) 141, 660, 1356, 1370
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 2 cut(s) 344, 1223
SmoI CTYRAG 2 cut(s) 344, 1223
SpeI ACTAGT 1 cut(s) 1078
SphI GCATGC 1 cut(s) 1379
Sse9I AATT 4 cut(s) 326, 361, 559, 1291
SsiI CCGC 7 cut(s) 185, 467, 668, 794, 1102, 1273, 1373
SspMI CTAG 6 cut(s) 171, 224, 255, 1079, 1173, 1347
StyD4I CCNGG 2 cut(s) 451, 1276
StyI CCWWGG 4 cut(s) 285, 674, 1051, 1167
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 7 cut(s) 322, 423, 760, 969, 985, 1075, 1090
TasI AATT 4 cut(s) 326, 361, 559, 1291
TatI WGTACW 1 cut(s) 197
TauI GCSGC 3 cut(s) 670, 1104, 1276
TfiI GAWTC 3 cut(s) 376, 643, 1068
Tru1I TTAA 4 cut(s) 345, 768, 1023, 1395
Tru9I TTAA 4 cut(s) 345, 768, 1023, 1395
TscAI CASTG 3 cut(s) 279, 357, 756
TseFI GTSAC 2 cut(s) 352, 542
TseI GCWGC 7 cut(s) 227, 290, 707, 896, 1142, 1204, 1207
Tsp45I GTSAC 2 cut(s) 352, 542
TspDTI ATGAA 7 cut(s) 14, 93, 99, 119, 441, 542, 704
TspRI CASTG 3 cut(s) 279, 357, 756
Van91I CCANNNNNTGG 1 cut(s) 1052
Vha464I CTTAAG 1 cut(s) 344
VpaK11BI GGWCC 4 cut(s) 141, 660, 1356, 1370
XapI RAATTY 3 cut(s) 326, 361, 1291
XceI RCATGY 1 cut(s) 1379
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XmiI GTMKAC 1 cut(s) 945
XspI CTAG 6 cut(s) 171, 224, 255, 1079, 1173, 1347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.