Rroxscaffold_1G00021930

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000641
Physical Location & Seq
Forward (+)
26993760 .. 26997410
3651 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00021930.1

Sequence Viewer

Length: 345 bp
ATGCCTCGAGGGAGCTTGGAGAACCACCTATTTAGGAGGTCCCTGCCTCTTCCATGGTCTATTAGAATGAAAATTGCATTGGGTGCGGCAAAAGGTCTTGCTTTTCTTCATGAGGAGGCAGAAAGACCTGTGATTTATCGTGACTTTAAAACCTCCAACATCTTATTGGATGCAGATTACAATGCCAAACTTTCTGATTTTGGACTTGCCAAAGATGGCCCAGAGGGTGATAAAACTCATGTCTCCACACGTGTGATGGGAACTTATGGTTATGCAGCTCCAGAATATGTAATGACTGATACAAGGATGCTAGTAAGACTTCATTCAAGTCCTCAACTCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

114

Amino Acids

12.81

Weight (kDa)

9.0

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 99 4.8e-14 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2 - 97 1e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 6
AciI CCGC 1 cut(s) 86
AcvI CACGTG 1 cut(s) 251
AflIII ACRYGT 2 cut(s) 248, 250
AgsI TTSAA 1 cut(s) 327
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 2 cut(s) 15, 278
AluI AGCT 2 cut(s) 15, 278
Alw26I GTCTC 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 1 cut(s) 217
ApeKI GCWGC 1 cut(s) 275
AspS9I GGNCC 2 cut(s) 39, 218
AsuHPI GGTGA 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 39
BbrPI CACGTG 1 cut(s) 251
BbvI GCAGC 1 cut(s) 287
BccI CCATC 2 cut(s) 209, 250
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 1 cut(s) 311
BisI GCNGC 2 cut(s) 87, 276
BlsI GCNGC 2 cut(s) 88, 277
Bme18I GGWCC 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 2 cut(s) 39, 218
BmiI GGNNCC 1 cut(s) 41
BmsI GCATC 2 cut(s) 160, 297
BpmI CTGGAG 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 2 cut(s) 175, 312
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 287
BshFI GGCC 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 25
BsnI GGCC 1 cut(s) 219
BsoBI CYCGRG 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 219
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 53
Bst6I CTCTTC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 251
BstDEI CTNAG 1 cut(s) 338
BstDSI CCRYGG 1 cut(s) 53
BstF5I GGATG 2 cut(s) 175, 312
BstMAI GTCTC 1 cut(s) 247
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 2 cut(s) 175, 312
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 39, 218
CviAII CATG 3 cut(s) 54, 110, 239
CviJI RGCY 3 cut(s) 15, 219, 278
CviKI_1 RGCY 3 cut(s) 15, 219, 278
DdeI CTNAG 1 cut(s) 338
DraI TTTAAA 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 39
Eco72I CACGTG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 3 cut(s) 57, 113, 242
FaiI YATR 6 cut(s) 55, 111, 240, 267, 273, 288
FaqI GGGAC 1 cut(s) 25
FatI CATG 3 cut(s) 53, 109, 238
Fnu4HI GCNGC 2 cut(s) 87, 276
FokI GGATG 2 cut(s) 182, 319
Fsp4HI GCNGC 2 cut(s) 87, 276
FspBI CTAG 1 cut(s) 311
GluI GCNGC 2 cut(s) 87, 276
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 1 cut(s) 219
Hin1II CATG 3 cut(s) 57, 113, 242
HphI GGTGA 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 3 cut(s) 110, 140, 281
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 3 cut(s) 77, 173, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 338
HpySE526I ACGT 1 cut(s) 250
Hsp92II CATG 3 cut(s) 57, 113, 242
LmnI GCTCC 2 cut(s) 12, 283
LpnPI CCDG 4 cut(s) 56, 141, 234, 294
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 2 cut(s) 160, 297
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 1 cut(s) 140
MboII GAAGA 2 cut(s) 41, 98
MluCI AATT 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 8 cut(s) 15, 30, 57, 106, 109, 163, 217, 342
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 53
NlaIII CATG 3 cut(s) 57, 113, 242
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 140
OliI CACNNNNGTG 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 6
PagI TCATGA 1 cut(s) 109
PkrI GCNGC 2 cut(s) 88, 277
PmaCI CACGTG 1 cut(s) 251
PmlI CACGTG 1 cut(s) 251
Ppu21I YACGTR 1 cut(s) 251
PpuMI RGGWCCY 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 39
PspCI CACGTG 1 cut(s) 251
PspN4I GGNNCC 1 cut(s) 41
PspPI GGNCC 2 cut(s) 39, 218
PspPPI RGGWCCY 1 cut(s) 39
PspXI VCTCGAGB 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 87, 276
Sau96I GGNCC 2 cut(s) 39, 218
SetI ASST 8 cut(s) 17, 30, 41, 97, 130, 155, 253, 280
SfaNI GCATC 2 cut(s) 160, 297
Sfr274I CTCGAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 39
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 1 cut(s) 72
SsiI CCGC 1 cut(s) 86
SspMI CTAG 1 cut(s) 311
StyI CCWWGG 1 cut(s) 53
TaiI ACGT 1 cut(s) 253
TaqI TCGA 1 cut(s) 7
TasI AATT 1 cut(s) 72
TauI GCSGC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 3 cut(s) 83, 98, 311
VpaK11BI GGWCC 1 cut(s) 39
XcmI CCANNNNNNNNNTGG 2 cut(s) 163, 253
XhoI CTCGAG 1 cut(s) 6
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.