Rh4DG385400

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000641
Physical Location & Seq
Reverse (-)
60242378 .. 60245611
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG385400.1

Sequence Viewer

Length: 1194 bp
ATGGGTTTGGGAGCTGAGAATGGGAAAGTGAAAGTGGAGTCTTGGGATATAAGCAAATCCAAGGGGAGGAAGAAGAAGATGGTAGTGGATGATGATGATGATGATGTGGATGTGGAGGAAGAGGAGACCGGGTGCTGGCTAAGGCTGAGGTTTTTTGGGAGCTGCATTTCTTCAAGATCCAAAGTTGATAGCTCAATTAGTGGCACCAGTACTCACTGTGAAATCAAATCTACAAATGATACAAGCAGAGACCAACCGACGGCTACTGCAGTGTCTAACTCAACCACTAGTAACACAGAAAGTAATGCATCCACTTCAAAATTTGAAGAGGAACTTAAAGTTGCTTCAAGGTTGCGAAAATTTGCTTTTAACGATCTTAAATTGGCAACAAGAAATTTCAGACCTGAAAGTCTTCTTGGTGAAGGTGGTTTTGGTTGTGTGTTCAAGGGGTGGATAGAAGAAAATGGAACTGCTCCAGTCAAACCTGGCACAGGACTTACTGTTGCGGTTAAAACTCTTAATCATGATGGGCTTCAGGGTCATAAAGAATGGCTGGATTACAATGCCAAACTTTCTGATTTTGGACTTGCCAAAGATGGCCCGGAGGGTGATAAAACTCATGTCTCCACACGTGTGATGGGAACTTATGGTTATGCAGCTCCAGAATATGTAATGACTGGACATCTTACATCGAGGAGTGATGTCTATAGTTTTGGCGTAGTTTTACTTGAGATGCTGACAGGCAGAAGATCTATGGACAAAAATCGACCTAATGGTGAACATAACCTTGTGGAATGGGCTCGACCACATCTAGGAGATAGAAGAAGATTCTACCGATTAATAGACCCTCGGCTGGAAGGTCACTTTTCAATAAAAGGTGTACAGAAAGCTGCACAACTAGCTGCCCATTGCCTTAGTCGAGATCCAAAAGCAAGGCCCCTAATGAGCGATGTTGTTGAAGCATTAAAGCCATTGCCAAACCTTAAGGATATGGCAAGCTCATCATATTATTTCCAGACCATGCAAGGTGACCGAGTTGGATCCAGTCCGAATACCAGAAATGGTGTCAGAACACAAGGAGGATTGCTTACAAGGAATGGGCACAGGAGCCTATCAATACCAAATGGCTCCCATGCTTCTCCATATCACCATCAATTTCCCCATCCATCTCCGAAACCTAATGGAAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

397

Amino Acids

43.77

Weight (kDa)

9.01

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 188 - 322 8.3e-14 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 188 - 320 4.8e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 203
AciI CCGC 1 cut(s) 506
AclWI GGATC 4 cut(s) 171, 917, 1035, 1048
AcsI RAATTY 3 cut(s) 320, 359, 394
AcuI CTGAAG 1 cut(s) 518
AcvI CACGTG 1 cut(s) 632
AfaI GTAC 2 cut(s) 211, 882
AfiI CCNNNNNNNGG 6 cut(s) 66, 135, 259, 491, 812, 853
AflII CTTAAG 1 cut(s) 983
AflIII ACRYGT 2 cut(s) 629, 631
AgsI TTSAA 7 cut(s) 174, 318, 326, 348, 445, 870, 959
AhlI ACTAGT 1 cut(s) 287
AjnI CCWGG 1 cut(s) 484
AjuI GAANNNNNNNTTGG 4 cut(s) 399, 414, 431, 446
AleI CACNNNNGTG 1 cut(s) 632
AluBI AGCT 7 cut(s) 14, 162, 192, 659, 890, 902, 999
AluI AGCT 7 cut(s) 14, 162, 192, 659, 890, 902, 999
Alw26I GTCTC 3 cut(s) 119, 243, 628
AlwI GGATC 4 cut(s) 171, 917, 1035, 1048
AoxI GGCC 2 cut(s) 598, 935
ApeKI GCWGC 4 cut(s) 162, 656, 890, 902
ApoI RAATTY 3 cut(s) 320, 359, 394
AseI ATTAAT 1 cut(s) 839
Asp700I GAANNNNTTC 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 599, 936
AsuC2I CCSGG 2 cut(s) 130, 602
AsuHPI GGTGA 5 cut(s) 431, 620, 788, 1040, 1139
BaeGI GKGCMC 1 cut(s) 1104
BamHI GGATCC 1 cut(s) 1040
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 802
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 1 cut(s) 404
BbvCI CCTCAGC 1 cut(s) 146
BbvI GCAGC 4 cut(s) 149, 668, 877, 889
BccI CCATC 7 cut(s) 73, 521, 590, 631, 1158, 1170, 1174
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 1 cut(s) 486
BcnI CCSGG 2 cut(s) 130, 602
BcoDI GTCTC 3 cut(s) 119, 243, 628
BcuI ACTAGT 1 cut(s) 287
BfaI CTAG 3 cut(s) 288, 812, 899
BfmI CTRYAG 2 cut(s) 267, 706
BfrI CTTAAG 1 cut(s) 983
BglII AGATCT 1 cut(s) 749
BisI GCNGC 4 cut(s) 163, 657, 891, 903
BlsI GCNGC 4 cut(s) 164, 658, 892, 904
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 3 cut(s) 130, 486, 602
BmgT120I GGNCC 2 cut(s) 599, 936
BmiI GGNNCC 5 cut(s) 205, 938, 1042, 1109, 1129
BmrFI CCNGG 3 cut(s) 130, 486, 602
BmsI GCATC 2 cut(s) 317, 723
BpiI GAAGAC 1 cut(s) 404
BpmI CTGGAG 2 cut(s) 459, 645
Bpu10I CCTNAGC 2 cut(s) 140, 146
BpuEI CTTGAG 1 cut(s) 749
BpuMI CCSGG 2 cut(s) 130, 602
BsaAI YACGTR 1 cut(s) 632
BsaI GGTCTC 2 cut(s) 119, 243
BsaJI CCNNGG 2 cut(s) 60, 848
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 135, 259, 491, 812, 853
Bse1I ACTGG 4 cut(s) 207, 476, 682, 1044
Bse3DI GCAATG 2 cut(s) 907, 971
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 60, 848
BseGI GGATG 4 cut(s) 94, 115, 308, 1162
BseLI CCNNNNNNNGG 6 cut(s) 66, 135, 259, 491, 812, 853
BseMI GCAATG 2 cut(s) 907, 971
BseMII CTCAG 2 cut(s) 6, 137
BseNI ACTGG 4 cut(s) 207, 476, 682, 1044
BseRI GAGGAG 2 cut(s) 137, 709
BseSI GKGCMC 1 cut(s) 1104
BseXI GCAGC 4 cut(s) 149, 668, 877, 889
BsgI GTGCAG 1 cut(s) 876
BshFI GGCC 2 cut(s) 600, 937
BshNI GGYRCC 1 cut(s) 203
BsiSI CCGG 2 cut(s) 129, 602
BslI CCNNNNNNNGG 6 cut(s) 66, 135, 259, 491, 812, 853
BsmAI GTCTC 3 cut(s) 119, 243, 628
BsnI GGCC 2 cut(s) 600, 937
Bso31I GGTCTC 2 cut(s) 119, 243
Bsp1286I GDGCHC 2 cut(s) 802, 1104
Bsp1407I TGTACA 1 cut(s) 880
Bsp143I GATC 5 cut(s) 176, 373, 749, 922, 1040
BspACI CCGC 1 cut(s) 506
BspANI GGCC 2 cut(s) 600, 937
BspCNI CTCAG 2 cut(s) 7, 138
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 5 cut(s) 205, 938, 1042, 1109, 1129
BspMAI CTGCAG 1 cut(s) 271
BspPI GGATC 4 cut(s) 171, 917, 1035, 1048
BspT107I GGYRCC 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 983
BspTNI GGTCTC 2 cut(s) 119, 243
BsrDI GCAATG 2 cut(s) 907, 971
BsrGI TGTACA 1 cut(s) 880
BsrI ACTGG 4 cut(s) 207, 476, 682, 1044
BssECI CCNNGG 2 cut(s) 60, 848
BssMI GATC 5 cut(s) 176, 373, 749, 922, 1040
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 2 cut(s) 218, 502
Bst6I CTCTTC 2 cut(s) 114, 321
BstAFI CTTAAG 1 cut(s) 983
BstAUI TGTACA 1 cut(s) 880
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 137, 997
BstDEI CTNAG 4 cut(s) 15, 140, 146, 914
BstEII GGTNACC 1 cut(s) 1028
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 4 cut(s) 94, 115, 308, 1162
BstKTI GATC 5 cut(s) 179, 376, 752, 925, 1043
BstMAI GTCTC 3 cut(s) 119, 243, 628
BstMBI GATC 5 cut(s) 176, 373, 749, 922, 1040
BstMWI GCNNNNNNNGC 1 cut(s) 899
BstNI CCWGG 1 cut(s) 486
BstPI GGTNACC 1 cut(s) 1028
BstSCI CCNGG 3 cut(s) 128, 484, 600
BstSFI CTRYAG 2 cut(s) 267, 706
BstSLI GKGCMC 1 cut(s) 1104
BstV1I GCAGC 4 cut(s) 149, 668, 877, 889
BstV2I GAAGAC 1 cut(s) 404
BstX2I RGATCY 4 cut(s) 176, 749, 922, 1040
BstYI RGATCY 4 cut(s) 176, 749, 922, 1040
BsuRI GGCC 2 cut(s) 600, 937
BtgZI GCGATG 1 cut(s) 963
BtsCI GGATG 4 cut(s) 94, 115, 308, 1162
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 214, 276
Cac8I GCNNGC 2 cut(s) 137, 997
CciI TCATGA 1 cut(s) 523
Cfr13I GGNCC 2 cut(s) 599, 936
Csp6I GTAC 2 cut(s) 210, 881
CviAII CATG 4 cut(s) 524, 620, 1021, 1133
CviQI GTAC 2 cut(s) 210, 881
DdeI CTNAG 4 cut(s) 15, 140, 146, 914
DpnI GATC 5 cut(s) 178, 375, 751, 924, 1042
DpnII GATC 5 cut(s) 176, 373, 749, 922, 1040
DrdI GACNNNNNNGTC 1 cut(s) 408
DseDI GACNNNNNNGTC 1 cut(s) 408
Eam1104I CTCTTC 2 cut(s) 114, 321
EarI CTCTTC 2 cut(s) 114, 321
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 802
Eco31I GGTCTC 2 cut(s) 119, 243
Eco57I CTGAAG 1 cut(s) 518
Eco72I CACGTG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 1028
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 936
EcoO65I GGTNACC 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 4 cut(s) 527, 623, 1024, 1136
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 4 cut(s) 523, 619, 1020, 1132
Fnu4HI GCNGC 4 cut(s) 163, 657, 891, 903
FokI GGATG 4 cut(s) 101, 122, 295, 1149
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 4 cut(s) 163, 657, 891, 903
FspBI CTAG 3 cut(s) 288, 812, 899
GluI GCNGC 4 cut(s) 163, 657, 891, 903
GsuI CTGGAG 2 cut(s) 459, 645
HaeIII GGCC 2 cut(s) 600, 937
HapII CCGG 2 cut(s) 129, 602
Hin1II CATG 4 cut(s) 527, 623, 1024, 1136
HinfI GANTC 2 cut(s) 38, 828
HpaII CCGG 2 cut(s) 129, 602
HphI GGTGA 5 cut(s) 431, 620, 788, 1040, 1139
Hpy166II GTNNAC 2 cut(s) 779, 881
Hpy188I TCNGA 5 cut(s) 401, 577, 1050, 1070, 1173
Hpy188III TCNNGA 5 cut(s) 174, 524, 662, 920, 1015
Hpy8I GTNNAC 2 cut(s) 779, 881
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 416, 851
HpyCH4III ACNGT 2 cut(s) 218, 502
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 6 cut(s) 165, 269, 308, 656, 893, 1024
HpyF10VI GCNNNNNNNGC 1 cut(s) 899
HpyF3I CTNAG 4 cut(s) 15, 140, 146, 914
HpySE526I ACGT 1 cut(s) 631
Hsp92II CATG 4 cut(s) 527, 623, 1024, 1136
Kzo9I GATC 5 cut(s) 176, 373, 749, 922, 1040
LmnI GCTCC 6 cut(s) 11, 159, 478, 664, 1107, 1133
Lsp1109I GCAGC 4 cut(s) 149, 668, 877, 889
LweI GCATC 2 cut(s) 317, 723
MaeI CTAG 3 cut(s) 288, 812, 899
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 3 cut(s) 290, 860, 1028
MalI GATC 5 cut(s) 178, 375, 751, 924, 1042
MboI GATC 5 cut(s) 176, 373, 749, 922, 1040
MflI RGATCY 4 cut(s) 176, 749, 922, 1040
MhlI GDGCHC 2 cut(s) 802, 1104
MluCI AATT 6 cut(s) 195, 320, 359, 380, 394, 1154
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 1018
MnlI CCTC 9 cut(s) 60, 109, 115, 141, 322, 598, 687, 858, 1073
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 9 cut(s) 336, 369, 378, 510, 519, 839, 965, 984, 1192
MslI CAYNNNNRTG 1 cut(s) 632
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 2 cut(s) 129, 602
MspR9I CCNGG 3 cut(s) 130, 486, 602
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 1 cut(s) 899
NciI CCSGG 2 cut(s) 130, 602
NdeII GATC 5 cut(s) 176, 373, 749, 922, 1040
NlaIII CATG 4 cut(s) 527, 623, 1024, 1136
NlaIV GGNNCC 5 cut(s) 205, 938, 1042, 1109, 1129
NmeAIII GCCGAG 1 cut(s) 829
NmuCI GTSAC 2 cut(s) 860, 1028
NsiI ATGCAT 1 cut(s) 310
OliI CACNNNNGTG 1 cut(s) 632
PagI TCATGA 1 cut(s) 523
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 1 cut(s) 828
PkrI GCNGC 4 cut(s) 164, 658, 892, 904
PleI GAGTC 1 cut(s) 46
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 632
PshBI ATTAAT 1 cut(s) 839
Psp6I CCWGG 1 cut(s) 484
PspCI CACGTG 1 cut(s) 632
PspEI GGTNACC 1 cut(s) 1028
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 5 cut(s) 205, 938, 1042, 1109, 1129
PspPI GGNCC 2 cut(s) 599, 936
PstI CTGCAG 1 cut(s) 271
PsuI RGATCY 4 cut(s) 176, 749, 922, 1040
RsaI GTAC 2 cut(s) 211, 882
RsaNI GTAC 2 cut(s) 210, 881
RseI CAYNNNNRTG 1 cut(s) 632
SaqAI TTAA 9 cut(s) 336, 369, 378, 510, 519, 839, 965, 984, 1192
SatI GCNGC 4 cut(s) 163, 657, 891, 903
Sau3AI GATC 5 cut(s) 176, 373, 749, 922, 1040
Sau96I GGNCC 2 cut(s) 599, 936
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 3 cut(s) 130, 486, 602
SduI GDGCHC 2 cut(s) 802, 1104
SfaNI GCATC 2 cut(s) 317, 723
SfcI CTRYAG 2 cut(s) 267, 706
SmiMI CAYNNNNRTG 1 cut(s) 632
SmlI CTYRAG 2 cut(s) 728, 983
SmoI CTYRAG 2 cut(s) 728, 983
SpeI ACTAGT 1 cut(s) 287
Sse9I AATT 6 cut(s) 195, 320, 359, 380, 394, 1154
SsiI CCGC 1 cut(s) 506
SspMI CTAG 3 cut(s) 288, 812, 899
StyD4I CCNGG 3 cut(s) 128, 484, 600
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 218, 502
TaiI ACGT 1 cut(s) 634
TaqI TCGA 4 cut(s) 692, 766, 802, 919
TaqII GACCGA 1 cut(s) 1047
TasI AATT 6 cut(s) 195, 320, 359, 380, 394, 1154
TatI WGTACW 2 cut(s) 209, 880
TfiI GAWTC 1 cut(s) 828
Tru1I TTAA 9 cut(s) 336, 369, 378, 510, 519, 839, 965, 984, 1192
Tru9I TTAA 9 cut(s) 336, 369, 378, 510, 519, 839, 965, 984, 1192
TscAI CASTG 2 cut(s) 221, 276
TseFI GTSAC 2 cut(s) 860, 1028
TseI GCWGC 4 cut(s) 162, 656, 890, 902
Tsp45I GTSAC 2 cut(s) 860, 1028
TspRI CASTG 2 cut(s) 221, 276
Vha464I CTTAAG 1 cut(s) 983
VspI ATTAAT 1 cut(s) 839
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 3 cut(s) 320, 359, 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 3 cut(s) 288, 812, 899
ZrmI AGTACT 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.