Rw4G032540

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000641
Physical Location & Seq
Reverse (-)
59627924 .. 59631132
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032540.1

Sequence Viewer

Length: 1461 bp
ATGGGTTTGGGAGCTGAGAATGGGAAAGTGAAAGTGGAGTCTTGGGATATAAGCAAATCCAAGGGGAGGAAGAAGAAGATGGTAGTGGATGATGATGATGATGATGTGGAGGAAGAGGAGCCCGGGTGCTGGCTAAGGCTGAGGTTTTTTGGGAGCTGCATTTCTTCAAGATCCAAAGTTGATAGCTCAATTAGTGGCACCAGCACTCACTGTGAAATCAAATCTACAAATGATACAAGCAGAGACCAACCGACGGCTACTGCAGTGTCTAACTCAACCACTAGTAACACAGAAAGTAATGCATCCACTTCAAAATTTGAAGAGGAACTTAAAGTTGCTTCAAGGTTGCGAAAATTTGCTTTTAACGATCTTAAATTGGCAACAAGAAATTTCAGACCTGAAAGTCTTCTTGGTGAAGGTGGTTTTGGTTGTGTGTTCAAGGGGTGGATAGAAGAAAATGGAACTGCTCCAGTCAAACCTGGCACAGGACTTACTGTTGCGGTTAAAACTCTTAATCATGATGGGCTTCAGGGTCATAAAGAATGGCTGGCTGAAGTAAATTTCCTTGGTGACCTTGTCCATCCAAACTTGGTAAAACTTATTGGATACTGCATTGAAGATGATCAAAGGCTGTTAGTGTATGAGTTCATGCCTCGAGGGAGCTTGGAGAACCACCTATTTAGGAGGTCCCTGCCTCTTCCATGGTCTATTAGAATGAAAATTGCATTGGGTGCGGCAAGAGGTCTTGCTTTTCTTCATGAGGAGGCAGAAAGACCTGTGATTTATCGTGACTTTAAAACCTCCAACATCTTATTGGATGCAGATTACAATGCCAAACTTTCTGATTTTGGACTTGCCAAAGATGGCCCGGAGGGTGATAAAACTCATGTCTCCACACGTGTGATGGGAACTTATGGTTATGCAGCTCCAGAATATGTAATGACTGGACATCTTACATCGAGGAGTGATGTCTATAGTTTTGGCGTAGTTTTACTTGAGATGCTGACAGGCAGAAGATCTATGGACAAAAATCGACCTAATGGTGAACATAACCTTGTGGAATGGGCTCGACCACATCTAGGAGATAGAAGAAGATTCTACCGATTAATAGACCCTCGGCTGGAAGGTCACTTTTCAATAAAAGGTGTACAGAAAGCTGCACAACTAGCTGCCCATTGCCTTAGTCGAGATCCAAAAGCAAGGCCCCTAATGAGCGATGTTGTCGAAGCATTAAAGCCATTGCCAAACCTTAAGGATATGGCAAGCTCATCATATTATTTCCAGACCATGCAAGGTGACCGAGTTGGATCCAGTCCGAACACCAGAAATGGTGTCAGAACACAAGGAGGATTGCTTACAAGGAATGGGCACAGGAGCCTATCAATACCAAATGGCTCCCATGCTTCTCCATATCACCATCAATTTCCCCATCCATCTCCGAAACCTAATGGAAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

486

Amino Acids

54.03

Weight (kDa)

8.92

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 134 - 409 4.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 135 - 411 1.5e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 402
AbsI CCTCGAGG 1 cut(s) 654
AccB1I GGYRCC 1 cut(s) 197
AciI CCGC 2 cut(s) 500, 734
AclWI GGATC 4 cut(s) 165, 1184, 1302, 1315
AcsI RAATTY 4 cut(s) 314, 353, 388, 559
AcuI CTGAAG 2 cut(s) 512, 573
AcvI CACGTG 1 cut(s) 899
AfaI GTAC 1 cut(s) 1149
AfiI CCNNNNNNNGG 6 cut(s) 66, 129, 253, 485, 1079, 1120
AflII CTTAAG 1 cut(s) 1250
AflIII ACRYGT 2 cut(s) 896, 898
AgsI TTSAA 7 cut(s) 168, 312, 320, 342, 439, 617, 1137
AhlI ACTAGT 1 cut(s) 281
AjnI CCWGG 1 cut(s) 478
AjuI GAANNNNNNNTTGG 6 cut(s) 393, 408, 425, 440, 710, 742
AleI CACNNNNGTG 1 cut(s) 899
AluBI AGCT 8 cut(s) 14, 156, 186, 663, 926, 1157, 1169, 1266
AluI AGCT 8 cut(s) 14, 156, 186, 663, 926, 1157, 1169, 1266
Alw26I GTCTC 2 cut(s) 237, 895
AlwI GGATC 4 cut(s) 165, 1184, 1302, 1315
Ama87I CYCGRG 2 cut(s) 122, 654
AoxI GGCC 2 cut(s) 865, 1202
ApeKI GCWGC 4 cut(s) 156, 923, 1157, 1169
ApoI RAATTY 4 cut(s) 314, 353, 388, 559
AseI ATTAAT 1 cut(s) 1106
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 687, 866, 1203
AsuC2I CCSGG 3 cut(s) 123, 124, 869
AsuHPI GGTGA 6 cut(s) 425, 581, 887, 1055, 1307, 1406
AvaI CYCGRG 2 cut(s) 122, 654
AvaII GGWCC 1 cut(s) 687
BaeGI GKGCMC 1 cut(s) 1371
BamHI GGATCC 1 cut(s) 1307
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 2 cut(s) 123, 1069
BbrPI CACGTG 1 cut(s) 899
BbsI GAAGAC 1 cut(s) 398
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 4 cut(s) 143, 935, 1144, 1156
BccI CCATC 8 cut(s) 73, 515, 588, 857, 898, 1425, 1437, 1441
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 1 cut(s) 480
BciVI GTATCC 1 cut(s) 599
BclI TGATCA 1 cut(s) 622
BcnI CCSGG 3 cut(s) 123, 124, 869
BcoDI GTCTC 2 cut(s) 237, 895
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 3 cut(s) 282, 1079, 1166
BfmI CTRYAG 2 cut(s) 261, 973
BfrI CTTAAG 1 cut(s) 1250
BfuI GTATCC 1 cut(s) 599
BglII AGATCT 1 cut(s) 1016
BisI GCNGC 5 cut(s) 157, 735, 924, 1158, 1170
BlsI GCNGC 5 cut(s) 158, 736, 925, 1159, 1171
Bme1390I CCNGG 4 cut(s) 123, 124, 480, 869
Bme18I GGWCC 1 cut(s) 687
BmeT110I CYCGRG 2 cut(s) 122, 654
BmgT120I GGNCC 3 cut(s) 687, 866, 1203
BmiI GGNNCC 7 cut(s) 120, 199, 689, 1205, 1309, 1376, 1396
BmrFI CCNGG 4 cut(s) 123, 124, 480, 869
BmsI GCATC 3 cut(s) 311, 808, 990
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 2 cut(s) 453, 912
Bpu10I CCTNAGC 2 cut(s) 134, 140
BpuEI CTTGAG 1 cut(s) 1016
BpuMI CCSGG 3 cut(s) 123, 124, 869
BsaAI YACGTR 1 cut(s) 899
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 5 cut(s) 60, 122, 565, 701, 1115
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 129, 253, 485, 1079, 1120
Bse1I ACTGG 3 cut(s) 470, 949, 1311
Bse3DI GCAATG 2 cut(s) 1174, 1238
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 5 cut(s) 60, 122, 565, 701, 1115
BseGI GGATG 5 cut(s) 94, 302, 580, 823, 1429
BseLI CCNNNNNNNGG 6 cut(s) 66, 129, 253, 485, 1079, 1120
BseMI GCAATG 2 cut(s) 1174, 1238
BseMII CTCAG 2 cut(s) 6, 131
BseNI ACTGG 3 cut(s) 470, 949, 1311
BseRI GAGGAG 3 cut(s) 131, 776, 976
BseSI GKGCMC 1 cut(s) 1371
BseXI GCAGC 4 cut(s) 143, 935, 1144, 1156
BsgI GTGCAG 1 cut(s) 1143
BshFI GGCC 2 cut(s) 867, 1204
BshNI GGYRCC 1 cut(s) 197
BsiHKCI CYCGRG 2 cut(s) 122, 654
BsiSI CCGG 2 cut(s) 123, 869
BslFI GGGAC 1 cut(s) 673
BslI CCNNNNNNNGG 6 cut(s) 66, 129, 253, 485, 1079, 1120
BsmAI GTCTC 2 cut(s) 237, 895
BsmFI GGGAC 1 cut(s) 673
BsnI GGCC 2 cut(s) 867, 1204
Bso31I GGTCTC 1 cut(s) 237
BsoBI CYCGRG 2 cut(s) 122, 654
Bsp1286I GDGCHC 3 cut(s) 123, 1069, 1371
Bsp1407I TGTACA 1 cut(s) 1147
Bsp143I GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
Bsp19I CCATGG 1 cut(s) 701
BspACI CCGC 2 cut(s) 500, 734
BspANI GGCC 2 cut(s) 867, 1204
BspCNI CTCAG 2 cut(s) 7, 132
BspHI TCATGA 2 cut(s) 517, 757
BspLI GGNNCC 7 cut(s) 120, 199, 689, 1205, 1309, 1376, 1396
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 4 cut(s) 165, 1184, 1302, 1315
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 1250
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 2 cut(s) 1174, 1238
BsrGI TGTACA 1 cut(s) 1147
BsrI ACTGG 3 cut(s) 470, 949, 1311
BssECI CCNNGG 5 cut(s) 60, 122, 565, 701, 1115
BssMI GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
BssT1I CCWWGG 3 cut(s) 60, 565, 701
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 212, 496
Bst6I CTCTTC 3 cut(s) 108, 315, 702
BstAFI CTTAAG 1 cut(s) 1250
BstAPI GCANNNNNTGC 1 cut(s) 731
BstAUI TGTACA 1 cut(s) 1147
BstBAI YACGTR 1 cut(s) 899
BstC8I GCNNGC 3 cut(s) 131, 549, 1264
BstDEI CTNAG 4 cut(s) 15, 134, 140, 1181
BstDSI CCRYGG 1 cut(s) 701
BstEII GGTNACC 2 cut(s) 569, 1295
BstENI CCTNNNNNAGG 1 cut(s) 483
BstF5I GGATG 5 cut(s) 94, 302, 580, 823, 1429
BstKTI GATC 6 cut(s) 173, 370, 625, 1019, 1192, 1310
BstMAI GTCTC 2 cut(s) 237, 895
BstMBI GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
BstMWI GCNNNNNNNGC 2 cut(s) 731, 1166
BstNI CCWGG 1 cut(s) 480
BstPI GGTNACC 2 cut(s) 569, 1295
BstSCI CCNGG 4 cut(s) 121, 122, 478, 867
BstSFI CTRYAG 2 cut(s) 261, 973
BstSLI GKGCMC 1 cut(s) 1371
BstV1I GCAGC 4 cut(s) 143, 935, 1144, 1156
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 4 cut(s) 170, 1016, 1189, 1307
BstYI RGATCY 4 cut(s) 170, 1016, 1189, 1307
BsuI GTATCC 1 cut(s) 599
BsuRI GGCC 2 cut(s) 867, 1204
BtgI CCRYGG 1 cut(s) 701
BtgZI GCGATG 1 cut(s) 1230
BtsCI GGATG 5 cut(s) 94, 302, 580, 823, 1429
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 208, 270
Cac8I GCNNGC 3 cut(s) 131, 549, 1264
CciI TCATGA 2 cut(s) 517, 757
Cfr13I GGNCC 3 cut(s) 687, 866, 1203
Cfr9I CCCGGG 1 cut(s) 122
Csp6I GTAC 1 cut(s) 1148
CviAII CATG 7 cut(s) 518, 649, 702, 758, 887, 1288, 1400
CviQI GTAC 1 cut(s) 1148
DdeI CTNAG 4 cut(s) 15, 134, 140, 1181
DpnI GATC 6 cut(s) 172, 369, 624, 1018, 1191, 1309
DpnII GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
DraI TTTAAA 1 cut(s) 796
DrdI GACNNNNNNGTC 1 cut(s) 402
DseDI GACNNNNNNGTC 1 cut(s) 402
Eam1104I CTCTTC 3 cut(s) 108, 315, 702
EarI CTCTTC 3 cut(s) 108, 315, 702
Eco130I CCWWGG 3 cut(s) 60, 565, 701
Eco24I GRGCYC 2 cut(s) 123, 1069
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 687
Eco57I CTGAAG 2 cut(s) 512, 573
Eco72I CACGTG 1 cut(s) 899
Eco88I CYCGRG 2 cut(s) 122, 654
Eco91I GGTNACC 2 cut(s) 569, 1295
EcoNI CCTNNNNNAGG 1 cut(s) 483
EcoO109I RGGNCCY 2 cut(s) 687, 1203
EcoO65I GGTNACC 2 cut(s) 569, 1295
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 60, 565, 701
EcoT22I ATGCAT 1 cut(s) 304
EcoT38I GRGCYC 2 cut(s) 123, 1069
ErhI CCWWGG 3 cut(s) 60, 565, 701
FaeI CATG 7 cut(s) 521, 652, 705, 761, 890, 1291, 1403
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 673
FatI CATG 7 cut(s) 517, 648, 701, 757, 886, 1287, 1399
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 5 cut(s) 157, 735, 924, 1158, 1170
FokI GGATG 5 cut(s) 101, 289, 567, 830, 1416
FriOI GRGCYC 2 cut(s) 123, 1069
Fsp4HI GCNGC 5 cut(s) 157, 735, 924, 1158, 1170
FspBI CTAG 3 cut(s) 282, 1079, 1166
GluI GCNGC 5 cut(s) 157, 735, 924, 1158, 1170
GsuI CTGGAG 2 cut(s) 453, 912
HaeIII GGCC 2 cut(s) 867, 1204
HapII CCGG 2 cut(s) 123, 869
Hin1II CATG 7 cut(s) 521, 652, 705, 761, 890, 1291, 1403
HinfI GANTC 2 cut(s) 38, 1095
HpaII CCGG 2 cut(s) 123, 869
HphI GGTGA 6 cut(s) 425, 581, 887, 1055, 1307, 1406
Hpy166II GTNNAC 2 cut(s) 1046, 1148
Hpy188I TCNGA 5 cut(s) 395, 844, 1317, 1337, 1440
Hpy188III TCNNGA 7 cut(s) 168, 518, 758, 788, 929, 1187, 1282
Hpy8I GTNNAC 2 cut(s) 1046, 1148
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 2 cut(s) 410, 1118
HpyCH4III ACNGT 2 cut(s) 212, 496
HpyCH4IV ACGT 1 cut(s) 898
HpyCH4V TGCA 9 cut(s) 159, 263, 302, 612, 725, 821, 923, 1160, 1291
HpyF10VI GCNNNNNNNGC 2 cut(s) 731, 1166
HpyF3I CTNAG 4 cut(s) 15, 134, 140, 1181
HpySE526I ACGT 1 cut(s) 898
Hsp92II CATG 7 cut(s) 521, 652, 705, 761, 890, 1291, 1403
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
LmnI GCTCC 8 cut(s) 11, 118, 153, 472, 660, 931, 1374, 1400
Lsp1109I GCAGC 4 cut(s) 143, 935, 1144, 1156
LweI GCATC 3 cut(s) 311, 808, 990
MaeI CTAG 3 cut(s) 282, 1079, 1166
MaeII ACGT 1 cut(s) 898
MaeIII GTNAC 5 cut(s) 284, 569, 788, 1127, 1295
MalI GATC 6 cut(s) 172, 369, 624, 1018, 1191, 1309
MboI GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
MflI RGATCY 4 cut(s) 170, 1016, 1189, 1307
MhlI GDGCHC 3 cut(s) 123, 1069, 1371
MluCI AATT 8 cut(s) 189, 314, 353, 374, 388, 559, 720, 1421
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 2 cut(s) 828, 1285
Mph1103I ATGCAT 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 899
MspCI CTTAAG 1 cut(s) 1250
MspI CCGG 2 cut(s) 123, 869
MspR9I CCNGG 4 cut(s) 123, 124, 480, 869
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 731, 1166
NciI CCSGG 3 cut(s) 123, 124, 869
NcoI CCATGG 1 cut(s) 701
NdeII GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
NlaIII CATG 7 cut(s) 521, 652, 705, 761, 890, 1291, 1403
NlaIV GGNNCC 7 cut(s) 120, 199, 689, 1205, 1309, 1376, 1396
NmeAIII GCCGAG 1 cut(s) 1096
NmuCI GTSAC 4 cut(s) 569, 788, 1127, 1295
NsiI ATGCAT 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 899
PaeR7I CTCGAG 1 cut(s) 654
PagI TCATGA 2 cut(s) 517, 757
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 1095
PflFI GACNNNGTC 1 cut(s) 575
PkrI GCNGC 5 cut(s) 158, 736, 925, 1159, 1171
PleI GAGTC 1 cut(s) 46
PmaCI CACGTG 1 cut(s) 899
PmlI CACGTG 1 cut(s) 899
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 899
PpuMI RGGWCCY 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 1106
Psp5II RGGWCCY 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 478
PspCI CACGTG 1 cut(s) 899
PspEI GGTNACC 2 cut(s) 569, 1295
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 7 cut(s) 120, 199, 689, 1205, 1309, 1376, 1396
PspPI GGNCC 3 cut(s) 687, 866, 1203
PspPPI RGGWCCY 1 cut(s) 687
PspXI VCTCGAGB 1 cut(s) 654
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 4 cut(s) 170, 1016, 1189, 1307
PsyI GACNNNGTC 1 cut(s) 575
RsaI GTAC 1 cut(s) 1149
RsaNI GTAC 1 cut(s) 1148
RseI CAYNNNNRTG 1 cut(s) 899
SatI GCNGC 5 cut(s) 157, 735, 924, 1158, 1170
Sau3AI GATC 6 cut(s) 170, 367, 622, 1016, 1189, 1307
Sau96I GGNCC 3 cut(s) 687, 866, 1203
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 4 cut(s) 123, 124, 480, 869
SduI GDGCHC 3 cut(s) 123, 1069, 1371
SfaNI GCATC 3 cut(s) 311, 808, 990
SfcI CTRYAG 2 cut(s) 261, 973
Sfr274I CTCGAG 1 cut(s) 654
SinI GGWCC 1 cut(s) 687
SlaI CTCGAG 1 cut(s) 654
SmaI CCCGGG 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 3 cut(s) 654, 995, 1250
SmoI CTYRAG 3 cut(s) 654, 995, 1250
SpeI ACTAGT 1 cut(s) 281
Sse9I AATT 8 cut(s) 189, 314, 353, 374, 388, 559, 720, 1421
SsiI CCGC 2 cut(s) 500, 734
SspMI CTAG 3 cut(s) 282, 1079, 1166
StyD4I CCNGG 4 cut(s) 121, 122, 478, 867
StyI CCWWGG 3 cut(s) 60, 565, 701
TaaI ACNGT 2 cut(s) 212, 496
TaiI ACGT 1 cut(s) 901
TaqI TCGA 6 cut(s) 655, 959, 1033, 1069, 1186, 1224
TaqII GACCGA 1 cut(s) 1314
TasI AATT 8 cut(s) 189, 314, 353, 374, 388, 559, 720, 1421
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 1 cut(s) 1095
TscAI CASTG 2 cut(s) 215, 270
TseFI GTSAC 4 cut(s) 569, 788, 1127, 1295
TseI GCWGC 4 cut(s) 156, 923, 1157, 1169
Tsp45I GTSAC 4 cut(s) 569, 788, 1127, 1295
TspDTI ATGAA 3 cut(s) 637, 731, 746
TspMI CCCGGG 1 cut(s) 122
TspRI CASTG 2 cut(s) 215, 270
Tth111I GACNNNGTC 1 cut(s) 575
Vha464I CTTAAG 1 cut(s) 1250
VpaK11BI GGWCC 1 cut(s) 687
VspI ATTAAT 1 cut(s) 1106
XagI CCTNNNNNAGG 1 cut(s) 483
XapI RAATTY 4 cut(s) 314, 353, 388, 559
XcmI CCANNNNNNNNNTGG 2 cut(s) 811, 901
XhoI CTCGAG 1 cut(s) 654
XmaI CCCGGG 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 3 cut(s) 282, 1079, 1166
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.