pycom13g06850

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000641
Physical Location & Seq
Reverse (-)
4532693 .. 4535821
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g06850.5

Sequence Viewer

Length: 1218 bp
ATGGGATTGAAAGCTGAGGATGGTAAGGTGGAGGCCTGGGACATTAGCAAATCGAAGGTGAGGAGGAAGAAGAAGCACAAAGCTGACGGGGATGATGATGACGACGTGGAAGAGGAGGGGAGTGGGTGTTGGGTTAGGCTGAGGTTCTTTGGGAGCTGCATTTCTTCAAGATCCAAAGTCGATAGCTCAACCAGTGGGACCAGTGCTGCTCACTGTGGTAAAATTAAATCTATAAATGATACGAGCAGAGACCAACCAACAGCTACTGTAGTCTCTTCGTCAACCACTAGTAATACAGAAAGTAATGCATCCACTTCCAAACTTGAAGAGGAACTTAAAGTTGCTTCACGGTTGCGAAAATTCACATTGATCGATCTTAAATTGGCAACAAGAAATTTCAGACCTGAAAGTCTCCTTGGTGAAGGTGGCTTTGGTCGCGCCTTCAAGGGGTGGATCGAAGAAAATGGAACTGCTCCAGTAAAACCTGGCACAGGTCTTACTGTTGCAGTGAAAACTCTTAACCATGATGGACTTCAGGGTCATAAAGAATGGCTGGCTGAAGTAAATTATCTTGGTGACCTTGTCCATCCAAACTTAGTTAAACTTATAGGATACTGCATTGAAGATGATCAATGGCTACTAGTGTATGAGTTCATGCCTCGAGGGAGCTTGGAGAACCATCTATTTAGGAGGTCGTTGCCTCTTCCATGGTCTATTCGAATGAAGATTGCATTAGGTGCTGCAAAGGGCCTTGCTTTTCTCCACGAGGAAGCAGAAAGACCTGCGATCTATCGTGACTTTAAAACCTCCAACATTTTATTGGACGCAGATTACAATGCCAAGCTGTCTGACTTTGGACTTGCCAAAGATGGTCCCGAGGGTGATAAAACTCATGTCTCCACCCGTGTGATGGGAACTTATGGTTATGCAGCTCCAGAATATGTAATGACTGGACATCTTACATCCAAGAGTGATGTCTATAGTTTTGGTGTGGTTTTACTCGAGATGCTGACTGGCAGAAGATCCATGGACAAAAATCGACCCAATGGCGAACATAACCTTGTGGAATGGGCACGGCCACATCTAGGAGAAAGAAGAAGATTCTACCGGTTGATAGACCCTCGGCTTGAAGGCCACTTTTCAATAAAAGGTGTTCAAAAAGCCGCACAACTAGCTGCTCACTGCCTTAGTCGATCGATCCAAAAGCCAGGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

406

Amino Acids

44.99

Weight (kDa)

8.88

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 408, 537
AbsI CCTCGAGG 1 cut(s) 660
Acc36I ACCTGC 1 cut(s) 790
AccII CGCG 1 cut(s) 438
AciI CCGC 1 cut(s) 1164
AclWI GGATC 4 cut(s) 165, 461, 1017, 1192
AcoI YGGCCR 1 cut(s) 1076
AcsI RAATTY 2 cut(s) 359, 394
AcuI CTGAAG 2 cut(s) 518, 579
AfiI CCNNNNNNNGG 4 cut(s) 447, 491, 910, 1085
AgeI ACCGGT 1 cut(s) 1107
AgsI TTSAA 8 cut(s) 10, 168, 326, 445, 623, 1130, 1143, 1157
AhlI ACTAGT 2 cut(s) 287, 640
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 3 cut(s) 35, 484, 1207
AjuI GAANNNNNNNTTGG 4 cut(s) 399, 414, 431, 446
AleI CACNNNNGTG 1 cut(s) 905
AluBI AGCT 9 cut(s) 14, 83, 156, 186, 263, 669, 844, 932, 1175
AluI AGCT 9 cut(s) 14, 83, 156, 186, 263, 669, 844, 932, 1175
Alw26I GTCTC 4 cut(s) 243, 277, 416, 901
AlwI GGATC 4 cut(s) 165, 461, 1017, 1192
AlwNI CAGNNNCTG 1 cut(s) 266
Ama87I CYCGRG 3 cut(s) 660, 875, 1001
AoxI GGCC 5 cut(s) 33, 748, 1076, 1132, 1210
ApeKI GCWGC 5 cut(s) 156, 206, 740, 929, 1175
ApoI RAATTY 2 cut(s) 359, 394
AsiGI ACCGGT 1 cut(s) 1107
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 4 cut(s) 198, 748, 872, 1211
AsuHPI GGTGA 4 cut(s) 70, 431, 587, 893
AsuII TTCGAA 1 cut(s) 718
AvaI CYCGRG 3 cut(s) 660, 875, 1001
AvaII GGWCC 2 cut(s) 198, 872
BaeGI GKGCMC 1 cut(s) 1075
BauI CACGAG 1 cut(s) 764
BbvCI CCTCAGC 2 cut(s) 15, 140
BbvI GCAGC 5 cut(s) 143, 193, 727, 941, 1162
BccI CCATC 6 cut(s) 14, 521, 594, 687, 863, 904
BceAI ACGGC 1 cut(s) 1091
BciT130I CCWGG 3 cut(s) 37, 486, 1209
BciVI GTATCC 1 cut(s) 605
BclI TGATCA 1 cut(s) 628
BcoDI GTCTC 4 cut(s) 243, 277, 416, 901
BcuI ACTAGT 2 cut(s) 287, 640
BfaI CTAG 4 cut(s) 288, 641, 1085, 1172
BfmI CTRYAG 2 cut(s) 267, 979
BfuAI ACCTGC 1 cut(s) 790
BfuI GTATCC 1 cut(s) 605
BisI GCNGC 6 cut(s) 157, 207, 741, 930, 1164, 1176
BlsI GCNGC 6 cut(s) 158, 208, 742, 931, 1165, 1177
Bme1390I CCNGG 3 cut(s) 37, 486, 1209
Bme18I GGWCC 2 cut(s) 198, 872
BmeT110I CYCGRG 3 cut(s) 660, 875, 1001
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 198, 748, 872, 1211
BmiI GGNNCC 2 cut(s) 199, 874
BmrFI CCNGG 3 cut(s) 37, 486, 1209
BmsI GCATC 2 cut(s) 317, 996
BpmI CTGGAG 2 cut(s) 459, 918
Bpu10I CCTNAGC 2 cut(s) 15, 140
Bpu14I TTCGAA 1 cut(s) 718
Bsa29I ATCGAT 2 cut(s) 372, 1196
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 6 cut(s) 36, 415, 707, 876, 1026, 1121
BsaWI WCCGGW 1 cut(s) 1107
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 4 cut(s) 447, 491, 910, 1085
Bse118I RCCGGY 1 cut(s) 1107
Bse1I ACTGG 5 cut(s) 192, 201, 476, 955, 1018
BseBI CCWGG 3 cut(s) 37, 486, 1209
BseCI ATCGAT 2 cut(s) 372, 1196
BseDI CCNNGG 6 cut(s) 36, 415, 707, 876, 1026, 1121
BseGI GGATG 5 cut(s) 25, 97, 308, 586, 962
BseLI CCNNNNNNNGG 4 cut(s) 447, 491, 910, 1085
BseMII CTCAG 2 cut(s) 6, 131
BseNI ACTGG 5 cut(s) 192, 201, 476, 955, 1018
BseRI GAGGAG 2 cut(s) 76, 128
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 5 cut(s) 143, 193, 727, 941, 1162
Bsh1236I CGCG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 1196
BshFI GGCC 5 cut(s) 35, 750, 1078, 1134, 1212
BshTI ACCGGT 1 cut(s) 1107
BshVI ATCGAT 2 cut(s) 372, 1196
BsiEI CGRYCG 1 cut(s) 1196
BsiHKCI CYCGRG 3 cut(s) 660, 875, 1001
BsiSI CCGG 1 cut(s) 1108
BslFI GGGAC 3 cut(s) 53, 211, 858
BslI CCNNNNNNNGG 4 cut(s) 447, 491, 910, 1085
BsmAI GTCTC 4 cut(s) 243, 277, 416, 901
BsmFI GGGAC 3 cut(s) 53, 211, 858
BsnI GGCC 5 cut(s) 35, 750, 1078, 1134, 1212
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 3 cut(s) 660, 875, 1001
Bsp119I TTCGAA 1 cut(s) 718
Bsp1286I GDGCHC 1 cut(s) 1075
Bsp143I GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
Bsp19I CCATGG 2 cut(s) 707, 1026
BspACI CCGC 1 cut(s) 1164
BspANI GGCC 5 cut(s) 35, 750, 1078, 1134, 1212
BspCNI CTCAG 2 cut(s) 7, 132
BspDI ATCGAT 2 cut(s) 372, 1196
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 199, 874
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 4 cut(s) 165, 461, 1017, 1192
BspT104I TTCGAA 1 cut(s) 718
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 1107
BsrI ACTGG 5 cut(s) 192, 201, 476, 955, 1018
BssAI RCCGGY 1 cut(s) 1107
BssECI CCNNGG 6 cut(s) 36, 415, 707, 876, 1026, 1121
BssMI GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
BssSI CACGAG 1 cut(s) 764
BssT1I CCWWGG 3 cut(s) 415, 707, 1026
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 3 cut(s) 37, 486, 1209
Bst4CI ACNGT 4 cut(s) 215, 268, 351, 502
Bst6I CTCTTC 4 cut(s) 105, 280, 321, 708
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 718
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 4 cut(s) 15, 140, 595, 1187
BstDSI CCRYGG 2 cut(s) 707, 1026
BstEII GGTNACC 1 cut(s) 575
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 5 cut(s) 25, 97, 308, 586, 962
BstFNI CGCG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 9 cut(s) 173, 372, 376, 456, 631, 789, 1025, 1196, 1200
BstMAI GTCTC 4 cut(s) 243, 277, 416, 901
BstMBI GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
BstMCI CGRYCG 1 cut(s) 1196
BstMWI GCNNNNNNNGC 3 cut(s) 435, 737, 1172
BstNI CCWGG 3 cut(s) 37, 486, 1209
BstPI GGTNACC 1 cut(s) 575
BstSCI CCNGG 3 cut(s) 35, 484, 1207
BstSFI CTRYAG 2 cut(s) 267, 979
BstSLI GKGCMC 1 cut(s) 1075
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 5 cut(s) 143, 193, 727, 941, 1162
BstX2I RGATCY 2 cut(s) 170, 1022
BstYI RGATCY 2 cut(s) 170, 1022
Bsu15I ATCGAT 2 cut(s) 372, 1196
BsuI GTATCC 1 cut(s) 605
BsuRI GGCC 5 cut(s) 35, 750, 1078, 1134, 1212
BsuTUI ATCGAT 2 cut(s) 372, 1196
BtgI CCRYGG 2 cut(s) 707, 1026
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 5 cut(s) 25, 97, 308, 586, 962
BtsI GCAGTG 2 cut(s) 513, 1180
BtsIMutI CAGTG 5 cut(s) 199, 208, 211, 513, 1180
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 266
CfoI GCGC 1 cut(s) 440
Cfr10I RCCGGY 1 cut(s) 1107
Cfr13I GGNCC 4 cut(s) 198, 748, 872, 1211
ClaI ATCGAT 2 cut(s) 372, 1196
CseI GACGC 1 cut(s) 833
CspAI ACCGGT 1 cut(s) 1107
CviAII CATG 5 cut(s) 524, 655, 708, 893, 1027
DdeI CTNAG 4 cut(s) 15, 140, 595, 1187
DpnI GATC 9 cut(s) 172, 371, 375, 455, 630, 788, 1024, 1195, 1199
DpnII GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
DraI TTTAAA 1 cut(s) 802
DrdI GACNNNNNNGTC 2 cut(s) 408, 537
DseDI GACNNNNNNGTC 2 cut(s) 408, 537
EaeI YGGCCR 1 cut(s) 1076
Eam1104I CTCTTC 4 cut(s) 105, 280, 321, 708
EarI CTCTTC 4 cut(s) 105, 280, 321, 708
Eco130I CCWWGG 3 cut(s) 415, 707, 1026
Eco147I AGGCCT 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 198, 872
Eco57I CTGAAG 2 cut(s) 518, 579
Eco88I CYCGRG 3 cut(s) 660, 875, 1001
Eco91I GGTNACC 1 cut(s) 575
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 2 cut(s) 748, 1211
EcoO65I GGTNACC 1 cut(s) 575
EcoRII CCWGG 3 cut(s) 35, 484, 1207
EcoT14I CCWWGG 3 cut(s) 415, 707, 1026
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 3 cut(s) 415, 707, 1026
FaeI CATG 5 cut(s) 527, 658, 711, 896, 1030
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 3 cut(s) 53, 211, 858
FatI CATG 5 cut(s) 523, 654, 707, 892, 1026
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 6 cut(s) 157, 207, 741, 930, 1164, 1176
FokI GGATG 5 cut(s) 32, 104, 295, 573, 949
Fsp4HI GCNGC 6 cut(s) 157, 207, 741, 930, 1164, 1176
FspBI CTAG 4 cut(s) 288, 641, 1085, 1172
GlaI GCGC 1 cut(s) 439
GluI GCNGC 6 cut(s) 157, 207, 741, 930, 1164, 1176
GsuI CTGGAG 2 cut(s) 459, 918
HaeIII GGCC 5 cut(s) 35, 750, 1078, 1134, 1212
HapII CCGG 1 cut(s) 1108
HgaI GACGC 1 cut(s) 833
HhaI GCGC 1 cut(s) 440
Hin1II CATG 5 cut(s) 527, 658, 711, 896, 1030
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 1 cut(s) 1101
HpaII CCGG 1 cut(s) 1108
HphI GGTGA 4 cut(s) 70, 431, 587, 893
Hpy166II GTNNAC 1 cut(s) 282
Hpy188I TCNGA 2 cut(s) 401, 850
Hpy188III TCNNGA 5 cut(s) 168, 794, 875, 935, 1003
Hpy8I GTNNAC 1 cut(s) 282
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 49, 416, 451, 1124
HpyCH4III ACNGT 4 cut(s) 215, 268, 351, 502
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 7 cut(s) 159, 308, 506, 618, 731, 743, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 737, 1172
HpyF3I CTNAG 4 cut(s) 15, 140, 595, 1187
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 5 cut(s) 527, 658, 711, 896, 1030
HspAI GCGC 1 cut(s) 438
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
LmnI GCTCC 4 cut(s) 153, 478, 666, 937
Lsp1109I GCAGC 5 cut(s) 143, 193, 727, 941, 1162
LweI GCATC 2 cut(s) 317, 996
MaeI CTAG 4 cut(s) 288, 641, 1085, 1172
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 575, 794
MalI GATC 9 cut(s) 172, 371, 375, 455, 630, 788, 1024, 1195, 1199
MboI GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
MflI RGATCY 2 cut(s) 170, 1022
MhlI GDGCHC 1 cut(s) 1075
MluCI AATT 5 cut(s) 222, 359, 380, 394, 565
MmeI TCCRAC 1 cut(s) 834
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 6 cut(s) 225, 336, 378, 519, 600, 801
MslI CAYNNNNRTG 1 cut(s) 905
MspI CCGG 1 cut(s) 1108
MspR9I CCNGG 3 cut(s) 37, 486, 1209
MvaI CCWGG 3 cut(s) 37, 486, 1209
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 3 cut(s) 435, 737, 1172
NcoI CCATGG 2 cut(s) 707, 1026
NdeII GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
NlaIII CATG 5 cut(s) 527, 658, 711, 896, 1030
NlaIV GGNNCC 2 cut(s) 199, 874
NmeAIII GCCGAG 1 cut(s) 1102
NmuCI GTSAC 2 cut(s) 575, 794
NsiI ATGCAT 1 cut(s) 310
NspV TTCGAA 1 cut(s) 718
OliI CACNNNNGTG 1 cut(s) 905
PaeR7I CTCGAG 2 cut(s) 660, 1001
PceI AGGCCT 1 cut(s) 35
PfeI GAWTC 1 cut(s) 1101
PflFI GACNNNGTC 1 cut(s) 581
PinAI ACCGGT 1 cut(s) 1107
PkrI GCNGC 6 cut(s) 158, 208, 742, 931, 1165, 1177
Ple19I CGATCG 1 cut(s) 1196
Psp6I CCWGG 3 cut(s) 35, 484, 1207
PspEI GGTNACC 1 cut(s) 575
PspGI CCWGG 3 cut(s) 35, 484, 1207
PspN4I GGNNCC 2 cut(s) 199, 874
PspPI GGNCC 4 cut(s) 198, 748, 872, 1211
PspXI VCTCGAGB 1 cut(s) 660
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 2 cut(s) 170, 1022
PsyI GACNNNGTC 1 cut(s) 581
PvuI CGATCG 1 cut(s) 1196
RseI CAYNNNNRTG 1 cut(s) 905
SaqAI TTAA 6 cut(s) 225, 336, 378, 519, 600, 801
SatI GCNGC 6 cut(s) 157, 207, 741, 930, 1164, 1176
Sau3AI GATC 9 cut(s) 170, 369, 373, 453, 628, 786, 1022, 1193, 1197
Sau96I GGNCC 4 cut(s) 198, 748, 872, 1211
ScrFI CCNGG 3 cut(s) 37, 486, 1209
SduI GDGCHC 1 cut(s) 1075
SfaNI GCATC 2 cut(s) 317, 996
SfcI CTRYAG 2 cut(s) 267, 979
Sfr274I CTCGAG 2 cut(s) 660, 1001
SfuI TTCGAA 1 cut(s) 718
SinI GGWCC 2 cut(s) 198, 872
SlaI CTCGAG 2 cut(s) 660, 1001
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 2 cut(s) 660, 1001
SmoI CTYRAG 2 cut(s) 660, 1001
SpeI ACTAGT 2 cut(s) 287, 640
Sse9I AATT 5 cut(s) 222, 359, 380, 394, 565
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 1 cut(s) 1164
SspMI CTAG 4 cut(s) 288, 641, 1085, 1172
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 3 cut(s) 35, 484, 1207
StyI CCWWGG 3 cut(s) 415, 707, 1026
TaaI ACNGT 4 cut(s) 215, 268, 351, 502
TaiI ACGT 1 cut(s) 108
TasI AATT 5 cut(s) 222, 359, 380, 394, 565
TauI GCSGC 1 cut(s) 1166
TfiI GAWTC 1 cut(s) 1101
Tru1I TTAA 6 cut(s) 225, 336, 378, 519, 600, 801
Tru9I TTAA 6 cut(s) 225, 336, 378, 519, 600, 801
TscAI CASTG 5 cut(s) 199, 208, 218, 513, 1187
TseFI GTSAC 2 cut(s) 575, 794
TseI GCWGC 5 cut(s) 156, 206, 740, 929, 1175
Tsp45I GTSAC 2 cut(s) 575, 794
TspDTI ATGAA 2 cut(s) 643, 737
TspRI CASTG 5 cut(s) 199, 208, 218, 513, 1187
Tth111I GACNNNGTC 1 cut(s) 581
VpaK11BI GGWCC 2 cut(s) 198, 872
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 2 cut(s) 359, 394
XcmI CCANNNNNNNNNTGG 2 cut(s) 817, 907
XhoI CTCGAG 2 cut(s) 660, 1001
XspI CTAG 4 cut(s) 288, 641, 1085, 1172
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.