Rh2DG632800
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000641
Physical Location & Seq
Reverse (-)
86194639 .. 86195574
936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG632800.1

Sequence Viewer

Length: 555 bp
ATGGGAACCTATGGTTATGCAGCCCCAGAATATGTGATGACTGGACATTTGACATCAAAGAGTGATGTCTACAGTTTTGGAGTAGTCCTACTTGAAATGCTAAATGGAAGAAGATCCATGGATAAAAATAGACCAAATGGGGAACACAATCTTGTGGAATGGGCGAGACAACTTTTTGGAGACAAGAGGAGGTTATTCCAGTTAATTGACCCCCGGCTCGAAGGCCATTTTTCAATCAAGGGTGCACAAAAAGCTATCCAACTGGCTGCCCGCTGCCTCAGCCGTGACCCAAAAGCCAGACCAATGATGAGTGAAGTTGTTGAAGCCCTAAAGCCTTTACCAAGCCTCAAGGACATGGCCAGTTCTTCTTATCACTTTCAAACCATGCAAGCAGCTCGTACCAGATCCAACTTGAATGCTAAGAATGGCATTAGAACACAAGCAATCTTTGTACCGAGGAATGGACAACCGGTAAGGAGCTTGTCAAGCCCAAAGGGTCCTCCTGCTTCTCCATGTTATCCTCAGAAATCACCTAAACCTAACTCGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

184

Amino Acids

20.53

Weight (kDa)

10.13

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 108 1.6e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 110 2.9e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 271
AclWI GGATC 2 cut(s) 108, 399
AcoI YGGCCR 1 cut(s) 357
AfaI GTAC 2 cut(s) 400, 453
AfiI CCNNNNNNNGG 1 cut(s) 461
AgeI ACCGGT 1 cut(s) 469
AgsI TTSAA 5 cut(s) 95, 234, 323, 380, 415
AluBI AGCT 3 cut(s) 254, 395, 480
AluI AGCT 3 cut(s) 254, 395, 480
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 160, 174
Alw44I GTGCAC 1 cut(s) 243
AlwI GGATC 2 cut(s) 108, 399
AoxI GGCC 2 cut(s) 223, 357
ApaLI GTGCAC 1 cut(s) 243
ApeKI GCWGC 4 cut(s) 20, 266, 273, 392
ArsI GACNNNNNNTTYG 2 cut(s) 31, 63
AsiGI ACCGGT 1 cut(s) 469
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 522
AvaII GGWCC 1 cut(s) 497
BaeGI GKGCMC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 247
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 4 cut(s) 32, 253, 260, 404
BceAI ACGGC 1 cut(s) 267
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 2 cut(s) 160, 174
BfmI CTRYAG 1 cut(s) 70
BisI GCNGC 4 cut(s) 21, 267, 274, 393
BlsI GCNGC 4 cut(s) 22, 268, 275, 394
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 2 cut(s) 7, 498
BmrFI CCNGG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 117, 212, 455
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse118I RCCGGY 1 cut(s) 469
Bse1I ACTGG 4 cut(s) 46, 199, 267, 360
BseDI CCNNGG 3 cut(s) 117, 212, 455
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMII CTCAG 2 cut(s) 292, 536
BseNI ACTGG 4 cut(s) 46, 199, 267, 360
BseRI GAGGAG 1 cut(s) 202
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 4 cut(s) 32, 253, 260, 404
BshFI GGCC 2 cut(s) 225, 359
BshTI ACCGGT 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 2 cut(s) 214, 470
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 2 cut(s) 160, 174
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 2 cut(s) 225, 359
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 2 cut(s) 113, 404
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 1 cut(s) 271
BspANI GGCC 2 cut(s) 225, 359
BspCNI CTCAG 2 cut(s) 291, 535
BspLI GGNNCC 2 cut(s) 7, 498
BspPI GGATC 2 cut(s) 108, 399
BsrFI RCCGGY 1 cut(s) 469
BsrI ACTGG 4 cut(s) 46, 199, 267, 360
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 117, 212, 455
BssMI GATC 2 cut(s) 113, 404
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 271, 390
BstDEI CTNAG 3 cut(s) 278, 420, 522
BstDSI CCRYGG 1 cut(s) 117
BstKTI GATC 2 cut(s) 116, 407
BstMAI GTCTC 2 cut(s) 160, 174
BstMBI GATC 2 cut(s) 113, 404
BstMWI GCNNNNNNNGC 3 cut(s) 251, 279, 486
BstSCI CCNGG 1 cut(s) 212
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 4 cut(s) 32, 253, 260, 404
BstX2I RGATCY 2 cut(s) 113, 404
BstYI RGATCY 2 cut(s) 113, 404
BsuRI GGCC 2 cut(s) 225, 359
BtgI CCRYGG 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 271, 390
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 399, 452
CspAI ACCGGT 1 cut(s) 469
CviAII CATG 4 cut(s) 118, 355, 385, 513
CviQI GTAC 2 cut(s) 399, 452
DdeI CTNAG 3 cut(s) 278, 420, 522
DpnI GATC 2 cut(s) 115, 406
DpnII GATC 2 cut(s) 113, 404
EaeI YGGCCR 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 4 cut(s) 121, 358, 388, 516
FaiI YATR 7 cut(s) 12, 18, 33, 119, 356, 386, 514
FatI CATG 4 cut(s) 117, 354, 384, 512
FauI CCCGC 1 cut(s) 278
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 4 cut(s) 21, 267, 274, 393
Fsp4HI GCNGC 4 cut(s) 21, 267, 274, 393
GluI GCNGC 4 cut(s) 21, 267, 274, 393
HaeIII GGCC 2 cut(s) 225, 359
HapII CCGG 2 cut(s) 214, 470
Hin1II CATG 4 cut(s) 121, 358, 388, 516
HpaII CCGG 2 cut(s) 214, 470
HphI GGTGA 1 cut(s) 522
Hpy166II GTNNAC 2 cut(s) 70, 245
Hpy188I TCNGA 1 cut(s) 525
Hpy8I GTNNAC 2 cut(s) 70, 245
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 1 cut(s) 74
HpyCH4V TGCA 3 cut(s) 20, 245, 388
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 279, 486
HpyF3I CTNAG 3 cut(s) 278, 420, 522
Hsp92II CATG 4 cut(s) 121, 358, 388, 516
Kzo9I GATC 2 cut(s) 113, 404
LmnI GCTCC 1 cut(s) 477
Lsp1109I GCAGC 4 cut(s) 32, 253, 260, 404
MaeIII GTNAC 1 cut(s) 284
MalI GATC 2 cut(s) 115, 406
MboI GATC 2 cut(s) 113, 404
MboII GAAGA 3 cut(s) 120, 123, 357
MflI RGATCY 2 cut(s) 113, 404
MhlI GDGCHC 1 cut(s) 247
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 1 cut(s) 204
MluNI TGGCCA 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 283, 432
MnlI CCTC 7 cut(s) 180, 183, 287, 356, 450, 510, 531
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 1 cut(s) 203
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 2 cut(s) 214, 470
MspR9I CCNGG 1 cut(s) 214
Mva1269I GAATGC 1 cut(s) 421
MwoI GCNNNNNNNGC 3 cut(s) 251, 279, 486
NciI CCSGG 1 cut(s) 214
NcoI CCATGG 1 cut(s) 117
NdeII GATC 2 cut(s) 113, 404
NlaIII CATG 4 cut(s) 121, 358, 388, 516
NlaIV GGNNCC 2 cut(s) 7, 498
NmuCI GTSAC 1 cut(s) 284
PctI GAATGC 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 469
PkrI GCNGC 4 cut(s) 22, 268, 275, 394
PpuMI RGGWCCY 1 cut(s) 497
Psp5II RGGWCCY 1 cut(s) 497
PspN4I GGNNCC 2 cut(s) 7, 498
PspPI GGNCC 1 cut(s) 497
PspPPI RGGWCCY 1 cut(s) 497
PsuI RGATCY 2 cut(s) 113, 404
RsaI GTAC 2 cut(s) 400, 453
RsaNI GTAC 2 cut(s) 399, 452
SaqAI TTAA 1 cut(s) 203
SatI GCNGC 4 cut(s) 21, 267, 274, 393
Sau3AI GATC 2 cut(s) 113, 404
Sau96I GGNCC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 214
SduI GDGCHC 1 cut(s) 247
SetI ASST 7 cut(s) 11, 194, 256, 397, 482, 535, 541
SfcI CTRYAG 1 cut(s) 70
SinI GGWCC 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 1 cut(s) 204
SsiI CCGC 1 cut(s) 271
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 74
TaqI TCGA 2 cut(s) 219, 545
TasI AATT 1 cut(s) 204
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 4 cut(s) 20, 266, 273, 392
Tsp45I GTSAC 1 cut(s) 284
VneI GTGCAC 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.