MD09G1048900.v1.1

receptor-like protein kinase At5g15080

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000641
Physical Location & Seq
Reverse (-)
3210536 .. 3214897
4362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1048900.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGGATTGGGACGTGGTGCTTCTGTTGTATCTAAGAATGTCGGAAAATCGAAGGGAAAGAAAAGGAAAGATGATCAAGATGAAGTGGCAGGATGTTGGATGAAGTTAAGGTTCATGGGGATGTGCATGTCTCCAAAGACCAAACTGAATACGCCTACAAGTGGAACTACCTCACCAGGGGAAAGTAAAGGTGCAAATGACTCGATCAAAGACCAGCCAGCTGCTCCGGTTGCTTCGTCTTCATCCACCAGTAACACAGGAAGTACTGTGTCTACTCCCAAGGAAGCTGAGGAACTGAAAGTTGCTTCTCAGCTACGAAAATTCACCTTTGATGAGCTTAAGTCTGTAACAAGGAATTTTAGACCTGTGAATCTTCTAGGCGAGGGTGGATTTGGTTGTGTATATAAGGGCTGGATTGACGAACATGGAACTACTCCAGTAAAACCTGGAGCTGGGCTTGCAGTTGCCGTAAAGACTCTGAACCATGAGGGACTTCAAGGTCATAAAGAATGGCTGGCTGAAGTTAATTATCTTGGCAACCTACTTCATCCAAATTTAGTCAAGCTATATGGTTATTGCATTGAGGATGATCAAAGGTTACTTGTCTATGAGTTTATGCCTCGAGGAAGCTTGGAGAATCACCTCTTTAGAAGGACCTTGCCGCTCCCTTGGCCTATCCGGATGAAAATAGCTCTTGGTGCTGCACAGGGTCTTACCTTTCTTCATGAGGAAGCCAAAAGGCCAGTGATTTATCGAGATTTCAAAACATCTAATATACTGCTGGATGCGGACTACACCGCTAAGCTTTCTGATTTTGGGCTCGCAAAAGATGCTCCAGAGGGAGACAAAACTCACGTCTCCACCCGAGTTATGGGCACTTACGGTTATGCAGCCCCTGAGTACGTAATGACAGGACACCTCACTTCAAAAAGCGATGTCTATAGCTTCGGGGTGGTTTTGCTTGAAATGTTGGTTGGTAAAAGATCTATGGACAAAAACCGACCCAATGGGGAGCATAACTTGGTTGAGTGGGCACGCCCGCATCTGGGAGACAGGCAAAGGTTCTACCGTCTAATAGATCCTAGACTTGAAGGCCACTTCTCTATCAAAGGTGCTCAGAAGGCGTTTCATTTGGCTGCCCGTTGTCTTAGCCGTGACCCCAAGGCTAGACCTCTGATGAGTGAAGTTGTTGAAGTGCTGAAGCCTCTTCCTGACCTCAATGACATGGCCAGATCCTCATATTACTACCAAGCCTTGCAAGCTCCTGGGTCTAATCCAAATTCTCGAAATGGCGGTAGAGGGCTGGTCAGGGTTTCAAGGAACGGACAACCAACGAGAACCCGATCCATGCCAAATGGTCCGCATGCCTCACCATTTCACCTATACCATCTTAACCGATCACCCAGACCTATTTCGCAATCTGCACAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

478

Amino Acids

52.73

Weight (kDa)

9.64

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 123 - 398 7.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 125 - 400 3.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 498
AbsI CCTCGAGG 1 cut(s) 621
AccBSI CCGCTC 1 cut(s) 664
AccI GTMKAC 1 cut(s) 272
AccIII TCCGGA 1 cut(s) 678
AciI CCGC 6 cut(s) 662, 788, 798, 1040, 1293, 1361
AclWI GGATC 3 cut(s) 1073, 1227, 1338
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 4 cut(s) 320, 355, 553, 1279
AcuI CTGAAG 2 cut(s) 540, 1220
AfaI GTAC 2 cut(s) 265, 902
AfiI CCNNNNNNNGG 6 cut(s) 161, 177, 452, 871, 1045, 1046
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 7 cut(s) 497, 763, 927, 965, 1091, 1193, 1317
AjiI CACGTC 2 cut(s) 14, 856
AjnI CCWGG 3 cut(s) 175, 445, 1264
AjuI GAANNNNNNNTTGG 2 cut(s) 957, 989
Alw21I GWGCWC 1 cut(s) 1117
Alw26I GTCTC 4 cut(s) 135, 837, 862, 1044
AlwI GGATC 3 cut(s) 1073, 1227, 1338
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 2 cut(s) 621, 864
Aor13HI TCCGGA 1 cut(s) 678
AoxI GGCC 4 cut(s) 671, 740, 1093, 1227
ApeKI GCWGC 4 cut(s) 221, 701, 890, 1136
ApoI RAATTY 4 cut(s) 320, 355, 553, 1279
ArsI GACNNNNNNTTYG 2 cut(s) 840, 872
AspS9I GGNCC 2 cut(s) 654, 1358
AsuHPI GGTGA 6 cut(s) 165, 316, 632, 1362, 1370, 1392
AvaI CYCGRG 2 cut(s) 621, 864
AvaII GGWCC 2 cut(s) 654, 1358
BaeGI GKGCMC 2 cut(s) 878, 1036
BalI TGGCCA 1 cut(s) 1229
BanII GRGCYC 1 cut(s) 822
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 1117
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 208, 688, 902, 1123
BccI CCATC 1 cut(s) 1395
BceAI ACGGC 2 cut(s) 452, 1137
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BciT130I CCWGG 3 cut(s) 177, 447, 1266
BclI TGATCA 2 cut(s) 73, 589
BcoDI GTCTC 4 cut(s) 135, 837, 862, 1044
BfaI CTAG 3 cut(s) 377, 1083, 1167
BfmI CTRYAG 1 cut(s) 940
BfrI CTTAAG 1 cut(s) 338
BglII AGATCT 1 cut(s) 983
BisI GCNGC 5 cut(s) 222, 662, 702, 891, 1137
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 5 cut(s) 223, 663, 703, 892, 1138
BmcAI AGTACT 1 cut(s) 265
Bme1390I CCNGG 3 cut(s) 177, 447, 1266
Bme18I GGWCC 2 cut(s) 654, 1358
BmeT110I CYCGRG 2 cut(s) 621, 864
BmgBI CACGTC 2 cut(s) 14, 856
BmgT120I GGNCC 2 cut(s) 654, 1358
BmrFI CCNGG 3 cut(s) 177, 447, 1266
BmsI GCATC 3 cut(s) 775, 820, 1051
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 3 cut(s) 420, 468, 819
Bpu10I CCTNAGC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 801
BsaAI YACGTR 1 cut(s) 904
BsaJI CCNNGG 5 cut(s) 176, 279, 668, 1161, 1265
BsaWI WCCGGW 2 cut(s) 226, 678
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 177, 452, 871, 1045, 1046
Bse1I ACTGG 3 cut(s) 249, 437, 743
BseAI TCCGGA 1 cut(s) 678
BseBI CCWGG 3 cut(s) 177, 447, 1266
BseDI CCNNGG 5 cut(s) 176, 279, 668, 1161, 1265
BseGI GGATG 8 cut(s) 98, 105, 126, 242, 547, 592, 687, 790
BseLI CCNNNNNNNGG 6 cut(s) 161, 177, 452, 871, 1045, 1046
BseMII CTCAG 4 cut(s) 279, 323, 888, 1130
BseNI ACTGG 3 cut(s) 249, 437, 743
BseSI GKGCMC 2 cut(s) 878, 1036
BseXI GCAGC 4 cut(s) 208, 688, 902, 1123
BseYI CCCAGC 1 cut(s) 452
BsgI GTGCAG 2 cut(s) 687, 1407
BshFI GGCC 4 cut(s) 673, 742, 1095, 1229
BsiHKAI GWGCWC 1 cut(s) 1117
BsiHKCI CYCGRG 2 cut(s) 621, 864
BsiSI CCGG 2 cut(s) 227, 679
BslFI GGGAC 2 cut(s) 24, 504
BslI CCNNNNNNNGG 6 cut(s) 161, 177, 452, 871, 1045, 1046
BsmAI GTCTC 4 cut(s) 135, 837, 862, 1044
BsmBI CGTCTC 1 cut(s) 862
BsmFI GGGAC 2 cut(s) 24, 504
BsnI GGCC 4 cut(s) 673, 742, 1095, 1229
BsoBI CYCGRG 2 cut(s) 621, 864
Bsp1286I GDGCHC 4 cut(s) 822, 878, 1036, 1117
Bsp13I TCCGGA 1 cut(s) 678
Bsp143I GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 6 cut(s) 662, 788, 798, 1040, 1293, 1361
BspANI GGCC 4 cut(s) 673, 742, 1095, 1229
BspCNI CTCAG 4 cut(s) 280, 322, 889, 1129
BspEI TCCGGA 1 cut(s) 678
BspHI TCATGA 1 cut(s) 724
BspPI GGATC 3 cut(s) 1073, 1227, 1338
BspTI CTTAAG 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 664
BsrI ACTGG 3 cut(s) 249, 437, 743
BssECI CCNNGG 5 cut(s) 176, 279, 668, 1161, 1265
BssMI GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
BssT1I CCWWGG 3 cut(s) 279, 668, 1161
Bst2UI CCWGG 3 cut(s) 177, 447, 1266
Bst4CI ACNGT 3 cut(s) 268, 884, 1070
Bst6I CTCTTC 1 cut(s) 1212
BstAFI CTTAAG 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 830
BstBAI YACGTR 1 cut(s) 904
BstC8I GCNNGC 8 cut(s) 219, 459, 516, 822, 1036, 1040, 1260, 1365
BstDEI CTNAG 7 cut(s) 33, 288, 309, 801, 897, 1116, 1148
BstF5I GGATG 8 cut(s) 98, 105, 126, 242, 547, 592, 687, 790
BstKTI GATC 8 cut(s) 76, 207, 592, 986, 1081, 1235, 1346, 1400
BstMAI GTCTC 4 cut(s) 135, 837, 862, 1044
BstMBI GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
BstMWI GCNNNNNNNGC 7 cut(s) 230, 458, 670, 698, 830, 1121, 1259
BstNI CCWGG 3 cut(s) 177, 447, 1266
BstNSI RCATGY 2 cut(s) 130, 1367
BstSCI CCNGG 3 cut(s) 175, 445, 1264
BstSFI CTRYAG 1 cut(s) 940
BstSLI GKGCMC 2 cut(s) 878, 1036
BstSNI TACGTA 1 cut(s) 904
BstV1I GCAGC 4 cut(s) 208, 688, 902, 1123
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 3 cut(s) 983, 1078, 1232
BstYI RGATCY 3 cut(s) 983, 1078, 1232
BsuRI GGCC 4 cut(s) 673, 742, 1095, 1229
BtgZI GCGATG 1 cut(s) 948
BtrI CACGTC 2 cut(s) 14, 856
BtsCI GGATG 8 cut(s) 98, 105, 126, 242, 547, 592, 687, 790
BtsIMutI CAGTG 1 cut(s) 750
Cac8I GCNNGC 8 cut(s) 219, 459, 516, 822, 1036, 1040, 1260, 1365
CaiI CAGNNNCTG 1 cut(s) 896
CciI TCATGA 1 cut(s) 724
Cfr13I GGNCC 2 cut(s) 654, 1358
Csp6I GTAC 2 cut(s) 264, 901
CviAII CATG 8 cut(s) 115, 127, 425, 485, 725, 1225, 1348, 1364
CviQI GTAC 2 cut(s) 264, 901
DdeI CTNAG 7 cut(s) 33, 288, 309, 801, 897, 1116, 1148
DpnI GATC 8 cut(s) 75, 206, 591, 985, 1080, 1234, 1345, 1399
DpnII GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
DrdI GACNNNNNNGTC 1 cut(s) 498
DseDI GACNNNNNNGTC 1 cut(s) 498
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 1 cut(s) 1212
EarI CTCTTC 1 cut(s) 1212
Eco105I TACGTA 1 cut(s) 904
Eco130I CCWWGG 3 cut(s) 279, 668, 1161
Eco24I GRGCYC 1 cut(s) 822
Eco47I GGWCC 2 cut(s) 654, 1358
Eco57I CTGAAG 2 cut(s) 540, 1220
Eco88I CYCGRG 2 cut(s) 621, 864
EcoO109I RGGNCCY 1 cut(s) 654
EcoRII CCWGG 3 cut(s) 175, 445, 1264
EcoT14I CCWWGG 3 cut(s) 279, 668, 1161
EcoT38I GRGCYC 1 cut(s) 822
ErhI CCWWGG 3 cut(s) 279, 668, 1161
Esp3I CGTCTC 1 cut(s) 862
FaeI CATG 8 cut(s) 118, 130, 428, 488, 728, 1228, 1351, 1367
FaqI GGGAC 2 cut(s) 24, 504
FatI CATG 8 cut(s) 114, 126, 424, 484, 724, 1224, 1347, 1363
FauI CCCGC 1 cut(s) 1047
FbaI TGATCA 2 cut(s) 73, 589
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 5 cut(s) 222, 662, 702, 891, 1137
FokI GGATG 8 cut(s) 105, 112, 133, 229, 534, 599, 694, 797
FriOI GRGCYC 1 cut(s) 822
Fsp4HI GCNGC 5 cut(s) 222, 662, 702, 891, 1137
FspBI CTAG 3 cut(s) 377, 1083, 1167
GluI GCNGC 5 cut(s) 222, 662, 702, 891, 1137
GsaI CCCAGC 1 cut(s) 456
GsuI CTGGAG 3 cut(s) 420, 468, 819
HaeIII GGCC 4 cut(s) 673, 742, 1095, 1229
HapII CCGG 2 cut(s) 227, 679
Hin1II CATG 8 cut(s) 118, 130, 428, 488, 728, 1228, 1351, 1367
HindIII AAGCTT 2 cut(s) 628, 803
HinfI GANTC 4 cut(s) 200, 370, 475, 637
HpaII CCGG 2 cut(s) 227, 679
HphI GGTGA 6 cut(s) 165, 316, 632, 1362, 1370, 1392
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 5 cut(s) 44, 480, 811, 1119, 1176
Hpy188III TCNNGA 7 cut(s) 77, 679, 725, 755, 836, 1211, 1284
Hpy8I GTNNAC 1 cut(s) 273
HpyAV CCTTC 4 cut(s) 46, 645, 1085, 1114
HpyCH4III ACNGT 3 cut(s) 268, 884, 1070
HpyCH4IV ACGT 3 cut(s) 13, 855, 903
HpyCH4V TGCA 8 cut(s) 126, 194, 461, 579, 704, 890, 1258, 1424
HpyF10VI GCNNNNNNNGC 7 cut(s) 230, 458, 670, 698, 830, 1121, 1259
HpyF3I CTNAG 7 cut(s) 33, 288, 309, 801, 897, 1116, 1148
HpySE526I ACGT 3 cut(s) 13, 855, 903
Hsp92II CATG 8 cut(s) 118, 130, 428, 488, 728, 1228, 1351, 1367
Kpn2I TCCGGA 1 cut(s) 678
Ksp22I TGATCA 2 cut(s) 73, 589
Kzo9I GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
LmnI GCTCC 6 cut(s) 229, 449, 669, 838, 1012, 1267
Lsp1109I GCAGC 4 cut(s) 208, 688, 902, 1123
LweI GCATC 3 cut(s) 775, 820, 1051
MaeI CTAG 3 cut(s) 377, 1083, 1167
MaeII ACGT 3 cut(s) 13, 855, 903
MaeIII GTNAC 4 cut(s) 251, 346, 597, 1154
MalI GATC 8 cut(s) 75, 206, 591, 985, 1080, 1234, 1345, 1399
MbiI CCGCTC 1 cut(s) 664
MboI GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
MboII GAAGA 4 cut(s) 231, 365, 713, 1199
MflI RGATCY 3 cut(s) 983, 1078, 1232
MhlI GDGCHC 4 cut(s) 822, 878, 1036, 1117
MlsI TGGCCA 1 cut(s) 1229
MluCI AATT 5 cut(s) 320, 355, 526, 553, 1279
MluNI TGGCCA 1 cut(s) 1229
MlyI GAGTC 2 cut(s) 194, 469
MmeI TCCRAC 2 cut(s) 22, 77
Mox20I TGGCCA 1 cut(s) 1229
MroI TCCGGA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 4 cut(s) 107, 339, 525, 1392
MslI CAYNNNNRTG 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 1229
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 2 cut(s) 227, 679
MspR9I CCNGG 3 cut(s) 177, 447, 1266
MvaI CCWGG 3 cut(s) 177, 447, 1266
MwoI GCNNNNNNNGC 7 cut(s) 230, 458, 670, 698, 830, 1121, 1259
NdeII GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
NlaIII CATG 8 cut(s) 118, 130, 428, 488, 728, 1228, 1351, 1367
NmuCI GTSAC 1 cut(s) 1154
NspI RCATGY 2 cut(s) 130, 1367
PaeI GCATGC 1 cut(s) 1367
PaeR7I CTCGAG 1 cut(s) 621
PagI TCATGA 1 cut(s) 724
PfeI GAWTC 2 cut(s) 370, 637
PkrI GCNGC 5 cut(s) 223, 663, 703, 892, 1138
PleI GAGTC 2 cut(s) 194, 469
PpsI GAGTC 2 cut(s) 194, 469
Ppu21I YACGTR 1 cut(s) 904
PpuMI RGGWCCY 1 cut(s) 654
Psp5II RGGWCCY 1 cut(s) 654
Psp6I CCWGG 3 cut(s) 175, 445, 1264
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 3 cut(s) 175, 445, 1264
PspPI GGNCC 2 cut(s) 654, 1358
PspPPI RGGWCCY 1 cut(s) 654
PspXI VCTCGAGB 1 cut(s) 621
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 3 cut(s) 983, 1078, 1232
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 2 cut(s) 265, 902
RsaNI GTAC 2 cut(s) 264, 901
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 4 cut(s) 107, 339, 525, 1392
SatI GCNGC 5 cut(s) 222, 662, 702, 891, 1137
Sau3AI GATC 8 cut(s) 73, 204, 589, 983, 1078, 1232, 1343, 1397
Sau96I GGNCC 2 cut(s) 654, 1358
ScaI AGTACT 1 cut(s) 265
SchI GAGTC 2 cut(s) 194, 469
ScrFI CCNGG 3 cut(s) 177, 447, 1266
SduI GDGCHC 4 cut(s) 822, 878, 1036, 1117
SfaNI GCATC 3 cut(s) 775, 820, 1051
SfcI CTRYAG 1 cut(s) 940
Sfr274I CTCGAG 1 cut(s) 621
SinI GGWCC 2 cut(s) 654, 1358
SlaI CTCGAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 338, 621
SmoI CTYRAG 2 cut(s) 338, 621
SnaBI TACGTA 1 cut(s) 904
SphI GCATGC 1 cut(s) 1367
Sse9I AATT 5 cut(s) 320, 355, 526, 553, 1279
SsiI CCGC 6 cut(s) 662, 788, 798, 1040, 1293, 1361
SspMI CTAG 3 cut(s) 377, 1083, 1167
StyD4I CCNGG 3 cut(s) 175, 445, 1264
StyI CCWWGG 3 cut(s) 279, 668, 1161
TaaI ACNGT 3 cut(s) 268, 884, 1070
TaiI ACGT 3 cut(s) 16, 858, 906
TaqI TCGA 5 cut(s) 50, 203, 622, 754, 1285
TasI AATT 5 cut(s) 320, 355, 526, 553, 1279
TatI WGTACW 1 cut(s) 263
TauI GCSGC 1 cut(s) 664
TfiI GAWTC 2 cut(s) 370, 637
Tru1I TTAA 4 cut(s) 107, 339, 525, 1392
Tru9I TTAA 4 cut(s) 107, 339, 525, 1392
TscAI CASTG 1 cut(s) 750
TseFI GTSAC 1 cut(s) 1154
TseI GCWGC 4 cut(s) 221, 701, 890, 1136
Tsp45I GTSAC 1 cut(s) 1154
TspDTI ATGAA 8 cut(s) 96, 103, 116, 231, 536, 698, 713, 1118
TspGWI ACGGA 1 cut(s) 1338
TspRI CASTG 1 cut(s) 750
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 654, 1358
XapI RAATTY 4 cut(s) 320, 355, 553, 1279
XceI RCATGY 2 cut(s) 130, 1367
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XhoI CTCGAG 1 cut(s) 621
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 3 cut(s) 377, 1083, 1167
ZrmI AGTACT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.