MD17G1047100.v1.1
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000641
Physical Location & Seq
Forward (+)
3445637 .. 3448628
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1047100.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGGGTTTGGTGCTTAATCCTAGCAAGGTGGAGAGTTGCGATAACAGTAGCAAATCGAAGGGAAGGAAGAAGAAGGATGATGCGGTGGAGGAGACTGGGTGTTGGGTTAAGCTCCGGTTTGGGACCTGCATGCCTTCAAGATCCAAAGTTGATAGCTCCATCACTGGAACTAGTGTTTCTTATGCGGAAACTAAATCGTCGAATGAGAAAAGTAGAGATCGAGAACTTACTCGTGGAGGGTCGTCTACAACTACTAGTAATGCCGGAAGTACTACATCCACGCCCTATTTTAGTGAGGAACTGAAGGTTGCTTCTCAGCTCCGAAAATTCACATTTAACGAACTTAAGTTGGCGACTAGAAACTTCAGACCGGAGAGCCTTCTTGGTGAGGGTGGATTTGGTTGTGTATTTAAGGGTTGGGTTGAGGAGAATGGAACTGCTCCTGTGAAACCTGGTACAGGGCTTACGGTTGCAGTTAAGACCCTCAACCATGATGGGCTTCAGGGTCACAAAGAATGGCTTGCTGAAATTTATTTTCTTGGTGATCTCGTCCATCCTAATTTGGTAAAATTGATTGGTTACTCGATTGAAGATGATCAGAGGTTGTTGGTCTATGAGTTTTTGCCTAGGGGAAGTTTGGAAAACCACCTTTTCAGAAGGTCCCTGCCTCTTCCGTGGTCTATTAGAATGAGAATTGCACTCGGTGCTGCAAAGGGTCTTGCCTTTCTTCATGAAGAAGTTCAAAAACCGGTCATATACCGTGACTTTAAAACATCTAATATCCTATTAGATGCAGATTACAATGCCAAGCTTTCGGATTTCGGACTTGCGAAAGATGGTCCTGAGGGTGATAAAACTCATGTTTCCACACGAGTCATGGGAACCTATGGTTATGCAGCCCCAGAGTACGTGATGACTGGTCATTTGACATCAAAGAGCGATGTCTATAGTTTTGGAGTAGTGCTGCTCGAAATGTTAAACGGCAGAAGATCCATGGACAAAAATAGACCAAATGGGGAGCACAATCTTGTGCAGTGGGCGAGACAACTCTTTGGCGACAAGAGGAGGTTATTCCAGTTAATTGACCCCCGTCTTGAAGGTCATTTTTCTATTAAAGGTGCACAAAAAGCAGTCCAGCTTGCCGCCCACTGCCTCAGTCGTGACCCGAAAGCTAGACCAATGATGAGTGAAGTTGTGGAAGCCCTAAAGCCTCTACAAAACCTCAAGGATATGGCCAGTTCTTCCTACCACTTCCAAAACATACAAGCAGCTCGTTCGAGGTCCCACTCGAGTTCTAAGAACGGCATCAGAACGCAGGCAGTATTCATGCCGAGAAACGGACAGCCCGTAAGGAGCTTGTCAAGCCCAAATGGTCCTCCGGCGTCTCCATATTATCCTCGAAAATCGCCTAAACCTAACTCAAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

477

Amino Acids

52.87

Weight (kDa)

9.59

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 125 - 400 2.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 126 - 402 5.2e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 245
AciI CCGC 3 cut(s) 83, 185, 1143
AclWI GGATC 2 cut(s) 135, 984
AcoI YGGCCR 1 cut(s) 1233
AcsI RAATTY 2 cut(s) 326, 528
AcuI CTGAAG 3 cut(s) 323, 349, 485
AcyI GRCGYC 1 cut(s) 1382
AdeI CACNNNGTG 1 cut(s) 704
AfaI GTAC 3 cut(s) 271, 457, 908
AfiI CCNNNNNNNGG 2 cut(s) 458, 1337
AflII CTTAAG 1 cut(s) 344
AgeI ACCGGT 1 cut(s) 748
AgsI TTSAA 4 cut(s) 138, 590, 743, 1097
AhlI ACTAGT 2 cut(s) 170, 254
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 8 cut(s) 112, 156, 319, 811, 1138, 1172, 1271, 1356
AluI AGCT 8 cut(s) 112, 156, 319, 811, 1138, 1172, 1271, 1356
Alw21I GWGCWC 2 cut(s) 1023, 1123
Alw26I GTCTC 3 cut(s) 86, 1036, 1389
Alw44I GTGCAC 1 cut(s) 1119
AlwI GGATC 2 cut(s) 135, 984
Ama87I CYCGRG 1 cut(s) 1288
AoxI GGCC 1 cut(s) 1233
ApaLI GTGCAC 1 cut(s) 1119
ApeKI GCWGC 4 cut(s) 707, 896, 964, 1268
ApoI RAATTY 2 cut(s) 326, 528
ArsI GACNNNNNNTTYG 2 cut(s) 907, 939
AsiGI ACCGGT 1 cut(s) 748
Asp700I GAANNNNTTC 1 cut(s) 738
AspA2I CCTAGG 1 cut(s) 626
AspS9I GGNCC 5 cut(s) 123, 660, 839, 1281, 1373
AsuHPI GGTGA 3 cut(s) 398, 554, 860
AvaI CYCGRG 1 cut(s) 1288
AvaII GGWCC 5 cut(s) 123, 660, 839, 1281, 1373
AvrII CCTAGG 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 1 cut(s) 1123
BalI TGGCCA 1 cut(s) 1235
BauI CACGAG 2 cut(s) 231, 870
Bbv12I GWGCWC 2 cut(s) 1023, 1123
BbvI GCAGC 4 cut(s) 694, 908, 951, 1280
BccI CCATC 4 cut(s) 167, 488, 561, 830
BceAI ACGGC 2 cut(s) 997, 1318
BcgI CGANNNNNNTGC 2 cut(s) 1257, 1291
BciT130I CCWGG 1 cut(s) 453
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 3 cut(s) 86, 1036, 1389
BcuI ACTAGT 2 cut(s) 170, 254
BfaI CTAG 6 cut(s) 21, 171, 255, 357, 627, 1173
BfmI CTRYAG 1 cut(s) 946
BfrI CTTAAG 1 cut(s) 344
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 5 cut(s) 708, 897, 965, 1143, 1269
BlnI CCTAGG 1 cut(s) 626
BlsI GCNGC 5 cut(s) 709, 898, 966, 1144, 1270
BmcAI AGTACT 1 cut(s) 271
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 5 cut(s) 123, 660, 839, 1281, 1373
BmeT110I CYCGRG 1 cut(s) 1288
BmgT120I GGNCC 5 cut(s) 123, 660, 839, 1281, 1373
BmiI GGNNCC 4 cut(s) 124, 662, 883, 1283
BmrFI CCNGG 1 cut(s) 453
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 3 cut(s) 70, 781, 1314
BmuI ACTGGG 1 cut(s) 105
BoxI GACNNNNGTC 1 cut(s) 1089
BpuEI CTTGAG 1 cut(s) 1208
BsaAI YACGTR 1 cut(s) 910
BsaHI GRCGYC 1 cut(s) 1382
BsaJI CCNNGG 3 cut(s) 626, 674, 993
BsaWI WCCGGW 3 cut(s) 114, 370, 748
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 1337
Bse118I RCCGGY 1 cut(s) 748
Bse1I ACTGG 5 cut(s) 100, 169, 922, 1075, 1236
Bse21I CCTNAGG 1 cut(s) 843
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 3 cut(s) 626, 674, 993
BseGI GGATG 3 cut(s) 82, 275, 553
BseLI CCNNNNNNNGG 2 cut(s) 458, 1337
BseMII CTCAG 3 cut(s) 329, 834, 1168
BseNI ACTGG 5 cut(s) 100, 169, 922, 1075, 1236
BseRI GAGGAG 3 cut(s) 104, 440, 1078
BseSI GKGCMC 1 cut(s) 1123
BseXI GCAGC 4 cut(s) 694, 908, 951, 1280
BsgI GTGCAG 1 cut(s) 1052
BshFI GGCC 1 cut(s) 1235
BshTI ACCGGT 1 cut(s) 748
BsiHKAI GWGCWC 2 cut(s) 1023, 1123
BsiHKCI CYCGRG 1 cut(s) 1288
BsiSI CCGG 5 cut(s) 115, 264, 371, 749, 1379
BslFI GGGAC 3 cut(s) 136, 646, 1267
BslI CCNNNNNNNGG 2 cut(s) 458, 1337
BsmAI GTCTC 3 cut(s) 86, 1036, 1389
BsmBI CGTCTC 1 cut(s) 1389
BsmFI GGGAC 3 cut(s) 136, 646, 1267
BsnI GGCC 1 cut(s) 1235
BsoBI CYCGRG 1 cut(s) 1288
Bsp1286I GDGCHC 2 cut(s) 1023, 1123
Bsp143I GATC 5 cut(s) 140, 217, 544, 595, 989
Bsp19I CCATGG 1 cut(s) 993
BspACI CCGC 3 cut(s) 83, 185, 1143
BspANI GGCC 1 cut(s) 1235
BspCNI CTCAG 3 cut(s) 328, 835, 1167
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 4 cut(s) 124, 662, 883, 1283
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 135, 984
BspTI CTTAAG 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 748
BsrI ACTGG 5 cut(s) 100, 169, 922, 1075, 1236
BssAI RCCGGY 1 cut(s) 748
BssECI CCNNGG 3 cut(s) 626, 674, 993
BssMI GATC 5 cut(s) 140, 217, 544, 595, 989
BssNI GRCGYC 1 cut(s) 1382
BssSI CACGAG 2 cut(s) 231, 870
BssT1I CCWWGG 2 cut(s) 626, 993
Bst2BI CACGAG 2 cut(s) 231, 870
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 3 cut(s) 47, 469, 761
Bst6I CTCTTC 1 cut(s) 675
BstACI GRCGYC 1 cut(s) 1382
BstAFI CTTAAG 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 910
BstC8I GCNNGC 4 cut(s) 131, 522, 1140, 1317
BstDEI CTNAG 4 cut(s) 315, 843, 1154, 1296
BstDSI CCRYGG 2 cut(s) 674, 993
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 3 cut(s) 82, 275, 553
BstKTI GATC 5 cut(s) 143, 220, 547, 598, 992
BstMAI GTCTC 3 cut(s) 86, 1036, 1389
BstMBI GATC 5 cut(s) 140, 217, 544, 595, 989
BstMWI GCNNNNNNNGC 3 cut(s) 704, 1127, 1362
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 1 cut(s) 133
BstPAI GACNNNNGTC 1 cut(s) 1089
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 946
BstSLI GKGCMC 1 cut(s) 1123
BstV1I GCAGC 4 cut(s) 694, 908, 951, 1280
BstX2I RGATCY 2 cut(s) 140, 989
BstYI RGATCY 2 cut(s) 140, 989
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 1 cut(s) 1235
BtgI CCRYGG 2 cut(s) 674, 993
BtgZI GCGATG 1 cut(s) 954
BtsCI GGATG 3 cut(s) 82, 275, 553
BtsI GCAGTG 2 cut(s) 1040, 1147
BtsIMutI CAGTG 3 cut(s) 162, 1040, 1147
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 4 cut(s) 131, 522, 1140, 1317
CciI TCATGA 1 cut(s) 730
Cfr10I RCCGGY 1 cut(s) 748
Cfr13I GGNCC 5 cut(s) 123, 660, 839, 1281, 1373
CseI GACGC 1 cut(s) 1371
CsiI ACCWGGT 1 cut(s) 451
Csp6I GTAC 3 cut(s) 270, 456, 907
CspAI ACCGGT 1 cut(s) 748
CviAII CATG 7 cut(s) 130, 491, 731, 860, 877, 994, 1327
CviQI GTAC 3 cut(s) 270, 456, 907
DdeI CTNAG 4 cut(s) 315, 843, 1154, 1296
DpnI GATC 5 cut(s) 142, 219, 546, 597, 991
DpnII GATC 5 cut(s) 140, 217, 544, 595, 989
DraI TTTAAA 1 cut(s) 769
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 1233
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
Eco130I CCWWGG 2 cut(s) 626, 993
Eco47I GGWCC 5 cut(s) 123, 660, 839, 1281, 1373
Eco57I CTGAAG 3 cut(s) 323, 349, 485
Eco81I CCTNAGG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 1288
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 3 cut(s) 123, 660, 1281
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 2 cut(s) 626, 993
ErhI CCWWGG 2 cut(s) 626, 993
Esp3I CGTCTC 1 cut(s) 1389
FaeI CATG 7 cut(s) 133, 494, 734, 863, 880, 997, 1330
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FaqI GGGAC 3 cut(s) 136, 646, 1267
FatI CATG 7 cut(s) 129, 490, 730, 859, 876, 993, 1326
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 5 cut(s) 708, 897, 965, 1143, 1269
FokI GGATG 3 cut(s) 89, 262, 540
Fsp4HI GCNGC 5 cut(s) 708, 897, 965, 1143, 1269
FspBI CTAG 6 cut(s) 21, 171, 255, 357, 627, 1173
GluI GCNGC 5 cut(s) 708, 897, 965, 1143, 1269
HaeIII GGCC 1 cut(s) 1235
HapII CCGG 5 cut(s) 115, 264, 371, 749, 1379
HgaI GACGC 1 cut(s) 1371
Hin1I GRCGYC 1 cut(s) 1382
Hin1II CATG 7 cut(s) 133, 494, 734, 863, 880, 997, 1330
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 1 cut(s) 873
HpaII CCGG 5 cut(s) 115, 264, 371, 749, 1379
HphI GGTGA 3 cut(s) 398, 554, 860
Hpy166II GTNNAC 2 cut(s) 246, 1121
Hpy188I TCNGA 7 cut(s) 323, 368, 600, 656, 817, 824, 1310
Hpy188III TCNNGA 6 cut(s) 138, 221, 731, 842, 1094, 1160
Hpy8I GTNNAC 2 cut(s) 246, 1121
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 8 cut(s) 52, 57, 67, 144, 298, 389, 651, 1091
HpyCH4III ACNGT 3 cut(s) 47, 469, 761
HpyCH4IV ACGT 1 cut(s) 909
HpyCH4V TGCA 8 cut(s) 129, 473, 698, 710, 794, 896, 1033, 1121
HpyF10VI GCNNNNNNNGC 3 cut(s) 704, 1127, 1362
HpyF3I CTNAG 4 cut(s) 315, 843, 1154, 1296
HpySE526I ACGT 1 cut(s) 909
Hsp92I GRCGYC 1 cut(s) 1382
Hsp92II CATG 7 cut(s) 133, 494, 734, 863, 880, 997, 1330
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 5 cut(s) 140, 217, 544, 595, 989
LmnI GCTCC 6 cut(s) 117, 161, 324, 445, 1018, 1353
Lsp1109I GCAGC 4 cut(s) 694, 908, 951, 1280
LweI GCATC 3 cut(s) 70, 781, 1314
MabI ACCWGGT 1 cut(s) 451
MaeI CTAG 6 cut(s) 21, 171, 255, 357, 627, 1173
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 4 cut(s) 506, 578, 761, 1160
MalI GATC 5 cut(s) 142, 219, 546, 597, 991
MboI GATC 5 cut(s) 140, 217, 544, 595, 989
MboII GAAGA 8 cut(s) 79, 82, 602, 662, 719, 746, 999, 1233
MflI RGATCY 2 cut(s) 140, 989
MhlI GDGCHC 2 cut(s) 1023, 1123
MlsI TGGCCA 1 cut(s) 1235
MluCI AATT 6 cut(s) 326, 528, 559, 569, 693, 1080
MluNI TGGCCA 1 cut(s) 1235
MlyI GAGTC 1 cut(s) 882
Mox20I TGGCCA 1 cut(s) 1235
MroXI GAANNNNTTC 1 cut(s) 738
MscI TGGCCA 1 cut(s) 1235
Msp20I TGGCCA 1 cut(s) 1235
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 5 cut(s) 115, 264, 371, 749, 1379
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 3 cut(s) 704, 1127, 1362
NcoI CCATGG 1 cut(s) 993
NdeII GATC 5 cut(s) 140, 217, 544, 595, 989
NlaIII CATG 7 cut(s) 133, 494, 734, 863, 880, 997, 1330
NlaIV GGNNCC 4 cut(s) 124, 662, 883, 1283
NmeAIII GCCGAG 1 cut(s) 1356
NmuCI GTSAC 3 cut(s) 506, 761, 1160
NspI RCATGY 1 cut(s) 133
PaeI GCATGC 1 cut(s) 133
PaeR7I CTCGAG 1 cut(s) 1288
PagI TCATGA 1 cut(s) 730
PcsI WCGNNNNNNNCGW 1 cut(s) 1344
PdmI GAANNNNTTC 1 cut(s) 738
PinAI ACCGGT 1 cut(s) 748
PkrI GCNGC 5 cut(s) 709, 898, 966, 1144, 1270
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
Ppu21I YACGTR 1 cut(s) 910
PpuMI RGGWCCY 3 cut(s) 123, 660, 1281
PshAI GACNNNNGTC 1 cut(s) 1089
Psp5II RGGWCCY 3 cut(s) 123, 660, 1281
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 4 cut(s) 124, 662, 883, 1283
PspPI GGNCC 5 cut(s) 123, 660, 839, 1281, 1373
PspPPI RGGWCCY 3 cut(s) 123, 660, 1281
PspXI VCTCGAGB 1 cut(s) 1288
PsuI RGATCY 2 cut(s) 140, 989
RsaI GTAC 3 cut(s) 271, 457, 908
RsaNI GTAC 3 cut(s) 270, 456, 907
SatI GCNGC 5 cut(s) 708, 897, 965, 1143, 1269
Sau3AI GATC 5 cut(s) 140, 217, 544, 595, 989
Sau96I GGNCC 5 cut(s) 123, 660, 839, 1281, 1373
ScaI AGTACT 1 cut(s) 271
SchI GAGTC 1 cut(s) 882
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 2 cut(s) 1023, 1123
SexAI ACCWGGT 1 cut(s) 451
SfaNI GCATC 3 cut(s) 70, 781, 1314
SfcI CTRYAG 1 cut(s) 946
Sfr274I CTCGAG 1 cut(s) 1288
SinI GGWCC 5 cut(s) 123, 660, 839, 1281, 1373
SlaI CTCGAG 1 cut(s) 1288
SmlI CTYRAG 3 cut(s) 344, 1223, 1288
SmoI CTYRAG 3 cut(s) 344, 1223, 1288
SpeI ACTAGT 2 cut(s) 170, 254
SphI GCATGC 1 cut(s) 133
Sse9I AATT 6 cut(s) 326, 528, 559, 569, 693, 1080
SsiI CCGC 3 cut(s) 83, 185, 1143
SspMI CTAG 6 cut(s) 21, 171, 255, 357, 627, 1173
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 2 cut(s) 626, 993
TaaI ACNGT 3 cut(s) 47, 469, 761
TaiI ACGT 1 cut(s) 912
TaqI TCGA 8 cut(s) 56, 200, 220, 584, 969, 1277, 1289, 1399
TasI AATT 6 cut(s) 326, 528, 559, 569, 693, 1080
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 1145
TscAI CASTG 3 cut(s) 169, 1040, 1154
TseFI GTSAC 3 cut(s) 506, 761, 1160
TseI GCWGC 4 cut(s) 707, 896, 964, 1268
Tsp45I GTSAC 3 cut(s) 506, 761, 1160
TspDTI ATGAA 3 cut(s) 719, 747, 1315
TspGWI ACGGA 2 cut(s) 663, 1353
TspRI CASTG 3 cut(s) 169, 1040, 1154
Vha464I CTTAAG 1 cut(s) 344
VneI GTGCAC 1 cut(s) 1119
VpaK11BI GGWCC 5 cut(s) 123, 660, 839, 1281, 1373
XagI CCTNNNNNAGG 1 cut(s) 456
XapI RAATTY 2 cut(s) 326, 528
XceI RCATGY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 1288
XmaJI CCTAGG 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 6 cut(s) 21, 171, 255, 357, 627, 1173
ZrmI AGTACT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.