MD17G1048900.v1.1

receptor-like protein kinase At5g15080

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000641
Physical Location & Seq
Reverse (-)
3557289 .. 3562021
4733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1048900.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGAATTGGGTCCTGATGCTTTTGTTGAAGCTAAGAATGTCGGGAAATCGAAGGGAAAGGAAAAGAAAGATGACGAAGATGAAGTGACAGGGTGTTGGATGAAGTTAAGGTTCATGGGGAGGTGCATGTCTCCAAAGACCAAACTTAATAGTTCTGCAAGTGGAACTAGCTCACCAGATGCAGAAAGTAAAGGTACACATGAATCGATCAAAGACCAACCAGGTGCTCCAGTTGCTTTGTCTTCAACCACCAGTAACACAGGAAGTACTGTGCCTACTCCTAAGGCAGCTGAGGAACTGAAAGTTGCTTCCCAGCTACGAAAGTTCACCTTTGATGAGCTCAAGTCTGTAACAAAGAATTTTAGACCTGTGAATCTTCTGGGCGAAGGTGGATTTGGTTGTGTATTTAAAGGCTGGATTGACGAACATGGAACTGCTCCAGTAAAACCTGGAGGTGGGCTTGCAGTTGCCGTAAAGACTCTGAACCATGACGGACTTCAAGGTCATAAAGAATGGCTGGCTGAAGTTAATTATCTTGGCGACCTACTTCATCCTAATTTAGTCAAACTATATGGTTATTGCATTGAGGATGATCAAAGGTTACTTGTATATGAGTTTATGCCTCGGGGAAGCTTGGAGAATCACCTCTTTAGAAAGTCCTGGCCGCTCCCTTGGTCTATCCGGATGAAAATAGCTCTTGGCGCTGCAAAGGGTCTTACCTTTCTTCATGAGGAAGCCGAAAGGCCAGTGATTTATCGAGATTTCAAAACATCTAATATATTGCTGGATGCGGACTACACTGCCAAGCTTTCTGATTTTGGGCTCGCCAAAGATGCTCCAGAGGGAGACAAAACTCACGTCTCCACCCGAGTTATGGGCACTTACGGTTATGCAGCCCCAGAGTATGTAATGACAGGACACCTCACTTCAAAAAGTGATGTGTATAGCTTCGGGGTGGTTCTGCTTGAAATGTTGGTTGGTAAAAGATCTATGGACAAAAACCGACCCAAAGGGGAGCATAACTTGGTTGAGTGGGCACACCCGCATCTGAGAGACAGGCGAAGGTTCTATCGTCTAGTAGATCCTCGAATTGAAGGCCGCTTCTCTATCAAAGGTGCTCAGAAGGCATTTCATTTGGCTGCCCGTTGTCTTAGCCGTGACCCCAAGGCTAGACCCCTGATGAGTGAAGTTGTTGAAGTGCTGAAGCCTCTTCCCGATCTCAAAGACATGGCCTGCTCCTCGTCTTACTACCAAGCCTTACAAGCTCCTGGGTCCAATCCAAATTCTCGAAATGGCAGCAGAGGGCTGGCTGGGGTTTCAAGGAATGGACAACCAACCAGAACCCGATCCATGCCAAATGGTCCGCATGCCTCACCATATCACCTAAACCATCTTAACCGATCACCCAGACCTATTGCGCAATCTGCACAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

479

Amino Acids

52.8

Weight (kDa)

9.32

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 124 - 399 2.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 126 - 401 5.8e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 501
Acc16I TGCGCA 1 cut(s) 1419
AccBSI CCGCTC 1 cut(s) 667
AccIII TCCGGA 1 cut(s) 681
AciI CCGC 5 cut(s) 665, 791, 1043, 1099, 1364
AclWI GGATC 2 cut(s) 1076, 1341
AcoI YGGCCR 1 cut(s) 662
AcsI RAATTY 2 cut(s) 358, 1282
AcuI CTGAAG 2 cut(s) 543, 1223
AfaI GTAC 2 cut(s) 196, 268
AfiI CCNNNNNNNGG 2 cut(s) 455, 874
AgsI TTSAA 9 cut(s) 29, 246, 500, 766, 930, 968, 1094, 1196, 1320
AjiI CACGTC 1 cut(s) 859
AjnI CCWGG 4 cut(s) 220, 448, 659, 1267
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 960, 992
Alw21I GWGCWC 3 cut(s) 229, 342, 1120
Alw26I GTCTC 4 cut(s) 135, 840, 865, 1047
AlwI GGATC 2 cut(s) 1076, 1341
Ama87I CYCGRG 2 cut(s) 624, 867
Aor13HI TCCGGA 1 cut(s) 681
AoxI GGCC 4 cut(s) 662, 743, 1096, 1230
ApeKI GCWGC 5 cut(s) 287, 704, 893, 1139, 1296
ApoI RAATTY 2 cut(s) 358, 1282
ArsI GACNNNNNNTTYG 2 cut(s) 843, 875
AspLEI GCGC 2 cut(s) 704, 1420
AspS9I GGNCC 3 cut(s) 11, 1272, 1361
AsuHPI GGTGA 6 cut(s) 165, 319, 635, 1365, 1373, 1395
AvaI CYCGRG 2 cut(s) 624, 867
AvaII GGWCC 3 cut(s) 11, 1272, 1361
AxyI CCTNAGG 1 cut(s) 282
BaeGI GKGCMC 2 cut(s) 881, 1039
BanII GRGCYC 2 cut(s) 342, 825
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 3 cut(s) 229, 342, 1120
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 5 cut(s) 299, 691, 905, 1126, 1308
BccI CCATC 1 cut(s) 1398
BceAI ACGGC 2 cut(s) 455, 1140
BciT130I CCWGG 4 cut(s) 222, 450, 661, 1269
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 4 cut(s) 135, 840, 865, 1047
BfaI CTAG 3 cut(s) 168, 1076, 1170
BfoI RGCGCY 1 cut(s) 705
BglII AGATCT 1 cut(s) 986
BisI GCNGC 7 cut(s) 288, 665, 705, 894, 1099, 1140, 1297
BlsI GCNGC 7 cut(s) 289, 666, 706, 895, 1100, 1141, 1298
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 4 cut(s) 222, 450, 661, 1269
Bme18I GGWCC 3 cut(s) 11, 1272, 1361
BmeT110I CYCGRG 2 cut(s) 624, 867
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 3 cut(s) 11, 1272, 1361
BmiI GGNNCC 2 cut(s) 12, 1273
BmrFI CCNGG 4 cut(s) 222, 450, 661, 1269
BmsI GCATC 5 cut(s) 7, 169, 778, 823, 1054
BpiI GAAGAC 1 cut(s) 234
BpmI CTGGAG 4 cut(s) 213, 423, 471, 822
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 206
BsaJI CCNNGG 4 cut(s) 623, 671, 1164, 1268
BsaWI WCCGGW 1 cut(s) 681
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 874
Bse1I ACTGG 4 cut(s) 230, 252, 440, 746
Bse21I CCTNAGG 1 cut(s) 282
BseAI TCCGGA 1 cut(s) 681
BseBI CCWGG 4 cut(s) 222, 450, 661, 1269
BseCI ATCGAT 1 cut(s) 206
BseDI CCNNGG 4 cut(s) 623, 671, 1164, 1268
BseGI GGATG 5 cut(s) 105, 550, 595, 690, 793
BseLI CCNNNNNNNGG 2 cut(s) 455, 874
BseMII CTCAG 3 cut(s) 282, 1040, 1133
BseNI ACTGG 4 cut(s) 230, 252, 440, 746
BseRI GAGGAG 1 cut(s) 1228
BseSI GKGCMC 2 cut(s) 881, 1039
BseXI GCAGC 5 cut(s) 299, 691, 905, 1126, 1308
BseYI CCCAGC 2 cut(s) 312, 1310
BsgI GTGCAG 1 cut(s) 1410
BshFI GGCC 4 cut(s) 664, 745, 1098, 1232
BshVI ATCGAT 1 cut(s) 206
BsiHKAI GWGCWC 3 cut(s) 229, 342, 1120
BsiHKCI CYCGRG 2 cut(s) 624, 867
BsiSI CCGG 1 cut(s) 682
BslI CCNNNNNNNGG 2 cut(s) 455, 874
BsmAI GTCTC 4 cut(s) 135, 840, 865, 1047
BsmBI CGTCTC 1 cut(s) 865
BsnI GGCC 4 cut(s) 664, 745, 1098, 1232
BsoBI CYCGRG 2 cut(s) 624, 867
Bsp1286I GDGCHC 6 cut(s) 229, 342, 825, 881, 1039, 1120
Bsp13I TCCGGA 1 cut(s) 681
Bsp143I GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
BspACI CCGC 5 cut(s) 665, 791, 1043, 1099, 1364
BspANI GGCC 4 cut(s) 664, 745, 1098, 1232
BspCNI CTCAG 3 cut(s) 283, 1041, 1132
BspDI ATCGAT 1 cut(s) 206
BspEI TCCGGA 1 cut(s) 681
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 2 cut(s) 12, 1273
BspPI GGATC 2 cut(s) 1076, 1341
BsrBI CCGCTC 1 cut(s) 667
BsrI ACTGG 4 cut(s) 230, 252, 440, 746
BssECI CCNNGG 4 cut(s) 623, 671, 1164, 1268
BssMI GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
BssT1I CCWWGG 2 cut(s) 671, 1164
Bst2UI CCWGG 4 cut(s) 222, 450, 661, 1269
Bst4CI ACNGT 2 cut(s) 271, 887
Bst6I CTCTTC 1 cut(s) 1215
BstC8I GCNNGC 6 cut(s) 462, 519, 825, 1234, 1308, 1368
BstDEI CTNAG 6 cut(s) 33, 282, 291, 1049, 1119, 1151
BstF5I GGATG 5 cut(s) 105, 550, 595, 690, 793
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 2 cut(s) 704, 1420
BstKTI GATC 7 cut(s) 210, 595, 989, 1084, 1219, 1349, 1403
BstMAI GTCTC 4 cut(s) 135, 840, 865, 1047
BstMBI GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
BstMWI GCNNNNNNNGC 6 cut(s) 233, 701, 833, 1124, 1262, 1424
BstNI CCWGG 4 cut(s) 222, 450, 661, 1269
BstNSI RCATGY 2 cut(s) 130, 1370
BstSCI CCNGG 4 cut(s) 220, 448, 659, 1267
BstSLI GKGCMC 2 cut(s) 881, 1039
BstV1I GCAGC 5 cut(s) 299, 691, 905, 1126, 1308
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 2 cut(s) 986, 1081
BstYI RGATCY 2 cut(s) 986, 1081
Bsu15I ATCGAT 1 cut(s) 206
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 4 cut(s) 664, 745, 1098, 1232
BsuTUI ATCGAT 1 cut(s) 206
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 5 cut(s) 105, 550, 595, 690, 793
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 2 cut(s) 753, 798
Cac8I GCNNGC 6 cut(s) 462, 519, 825, 1234, 1308, 1368
CciI TCATGA 1 cut(s) 727
CfoI GCGC 2 cut(s) 704, 1420
Cfr13I GGNCC 3 cut(s) 11, 1272, 1361
ClaI ATCGAT 1 cut(s) 206
CsiI ACCWGGT 1 cut(s) 220
Csp6I GTAC 2 cut(s) 195, 267
CviAII CATG 9 cut(s) 115, 127, 200, 428, 488, 728, 1228, 1351, 1367
CviQI GTAC 2 cut(s) 195, 267
DdeI CTNAG 6 cut(s) 33, 282, 291, 1049, 1119, 1151
DpnI GATC 7 cut(s) 209, 594, 988, 1083, 1218, 1348, 1402
DpnII GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
DraI TTTAAA 1 cut(s) 409
DrdI GACNNNNNNGTC 1 cut(s) 501
DseDI GACNNNNNNGTC 1 cut(s) 501
EaeI YGGCCR 1 cut(s) 662
Eam1104I CTCTTC 1 cut(s) 1215
EarI CTCTTC 1 cut(s) 1215
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 2 cut(s) 671, 1164
Eco24I GRGCYC 2 cut(s) 342, 825
Eco47I GGWCC 3 cut(s) 11, 1272, 1361
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 543, 1223
Eco81I CCTNAGG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 624, 867
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 4 cut(s) 220, 448, 659, 1267
EcoT14I CCWWGG 2 cut(s) 671, 1164
EcoT38I GRGCYC 2 cut(s) 342, 825
ErhI CCWWGG 2 cut(s) 671, 1164
Esp3I CGTCTC 1 cut(s) 865
FaeI CATG 9 cut(s) 118, 130, 203, 431, 491, 731, 1231, 1354, 1370
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FatI CATG 9 cut(s) 114, 126, 199, 427, 487, 727, 1227, 1350, 1366
FauI CCCGC 1 cut(s) 1050
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 7 cut(s) 288, 665, 705, 894, 1099, 1140, 1297
FokI GGATG 5 cut(s) 112, 537, 602, 697, 800
FriOI GRGCYC 2 cut(s) 342, 825
Fsp4HI GCNGC 7 cut(s) 288, 665, 705, 894, 1099, 1140, 1297
FspBI CTAG 3 cut(s) 168, 1076, 1170
FspI TGCGCA 1 cut(s) 1419
GlaI GCGC 2 cut(s) 703, 1419
GluI GCNGC 7 cut(s) 288, 665, 705, 894, 1099, 1140, 1297
GsaI CCCAGC 2 cut(s) 316, 1314
GsuI CTGGAG 4 cut(s) 213, 423, 471, 822
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 4 cut(s) 664, 745, 1098, 1232
HapII CCGG 1 cut(s) 682
HhaI GCGC 2 cut(s) 704, 1420
Hin1II CATG 9 cut(s) 118, 130, 203, 431, 491, 731, 1231, 1354, 1370
Hin6I GCGC 2 cut(s) 702, 1418
HinP1I GCGC 2 cut(s) 702, 1418
HindIII AAGCTT 2 cut(s) 631, 806
HinfI GANTC 4 cut(s) 203, 373, 478, 640
HpaII CCGG 1 cut(s) 682
HphI GGTGA 6 cut(s) 165, 319, 635, 1365, 1373, 1395
Hpy166II GTNNAC 2 cut(s) 197, 327
Hpy188I TCNGA 4 cut(s) 483, 814, 1050, 1122
Hpy188III TCNNGA 8 cut(s) 14, 43, 682, 728, 758, 839, 1214, 1287
Hpy8I GTNNAC 2 cut(s) 197, 327
HpyAV CCTTC 5 cut(s) 46, 380, 1056, 1088, 1117
HpyCH4III ACNGT 2 cut(s) 271, 887
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 8 cut(s) 126, 158, 182, 464, 582, 707, 893, 1427
HpyF10VI GCNNNNNNNGC 6 cut(s) 233, 701, 833, 1124, 1262, 1424
HpyF3I CTNAG 6 cut(s) 33, 282, 291, 1049, 1119, 1151
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 9 cut(s) 118, 130, 203, 431, 491, 731, 1231, 1354, 1370
HspAI GCGC 2 cut(s) 702, 1418
Kpn2I TCCGGA 1 cut(s) 681
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
LmnI GCTCC 7 cut(s) 232, 442, 672, 841, 1015, 1241, 1270
Lsp1109I GCAGC 5 cut(s) 299, 691, 905, 1126, 1308
LweI GCATC 5 cut(s) 7, 169, 778, 823, 1054
MabI ACCWGGT 1 cut(s) 220
MaeI CTAG 3 cut(s) 168, 1076, 1170
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 5 cut(s) 85, 254, 349, 600, 1157
MalI GATC 7 cut(s) 209, 594, 988, 1083, 1218, 1348, 1402
MbiI CCGCTC 1 cut(s) 667
MboI GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
MboII GAAGA 5 cut(s) 89, 234, 368, 716, 1202
MflI RGATCY 2 cut(s) 986, 1081
MhlI GDGCHC 6 cut(s) 229, 342, 825, 881, 1039, 1120
MluCI AATT 6 cut(s) 5, 358, 529, 556, 1089, 1282
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 77
MroI TCCGGA 1 cut(s) 681
MseI TTAA 5 cut(s) 107, 147, 408, 528, 1395
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 4 cut(s) 222, 450, 661, 1269
MvaI CCWGG 4 cut(s) 222, 450, 661, 1269
MwoI GCNNNNNNNGC 6 cut(s) 233, 701, 833, 1124, 1262, 1424
NdeII GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
NlaIII CATG 9 cut(s) 118, 130, 203, 431, 491, 731, 1231, 1354, 1370
NlaIV GGNNCC 2 cut(s) 12, 1273
NmuCI GTSAC 2 cut(s) 85, 1157
NsbI TGCGCA 1 cut(s) 1419
NspI RCATGY 2 cut(s) 130, 1370
PaeI GCATGC 1 cut(s) 1370
PagI TCATGA 1 cut(s) 727
PfeI GAWTC 3 cut(s) 203, 373, 640
PkrI GCNGC 7 cut(s) 289, 666, 706, 895, 1100, 1141, 1298
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PpuMI RGGWCCY 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 4 cut(s) 220, 448, 659, 1267
PspFI CCCAGC 2 cut(s) 312, 1310
PspGI CCWGG 4 cut(s) 220, 448, 659, 1267
PspN4I GGNNCC 2 cut(s) 12, 1273
PspPI GGNCC 3 cut(s) 11, 1272, 1361
PspPPI RGGWCCY 1 cut(s) 11
PsuI RGATCY 2 cut(s) 986, 1081
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 196, 268
RsaNI GTAC 2 cut(s) 195, 267
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 5 cut(s) 107, 147, 408, 528, 1395
SatI GCNGC 7 cut(s) 288, 665, 705, 894, 1099, 1140, 1297
Sau3AI GATC 7 cut(s) 207, 592, 986, 1081, 1216, 1346, 1400
Sau96I GGNCC 3 cut(s) 11, 1272, 1361
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 4 cut(s) 222, 450, 661, 1269
SduI GDGCHC 6 cut(s) 229, 342, 825, 881, 1039, 1120
SexAI ACCWGGT 1 cut(s) 220
SfaNI GCATC 5 cut(s) 7, 169, 778, 823, 1054
SinI GGWCC 3 cut(s) 11, 1272, 1361
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SphI GCATGC 1 cut(s) 1370
Sse9I AATT 6 cut(s) 5, 358, 529, 556, 1089, 1282
SsiI CCGC 5 cut(s) 665, 791, 1043, 1099, 1364
SspMI CTAG 3 cut(s) 168, 1076, 1170
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 4 cut(s) 220, 448, 659, 1267
StyI CCWWGG 2 cut(s) 671, 1164
TaaI ACNGT 2 cut(s) 271, 887
TaiI ACGT 1 cut(s) 861
TaqI TCGA 5 cut(s) 50, 206, 757, 1087, 1288
TasI AATT 6 cut(s) 5, 358, 529, 556, 1089, 1282
TatI WGTACW 1 cut(s) 266
TauI GCSGC 2 cut(s) 667, 1101
TfiI GAWTC 3 cut(s) 203, 373, 640
Tru1I TTAA 5 cut(s) 107, 147, 408, 528, 1395
Tru9I TTAA 5 cut(s) 107, 147, 408, 528, 1395
TscAI CASTG 2 cut(s) 753, 805
TseFI GTSAC 2 cut(s) 85, 1157
TseI GCWGC 5 cut(s) 287, 704, 893, 1139, 1296
Tsp45I GTSAC 2 cut(s) 85, 1157
TspDTI ATGAA 8 cut(s) 96, 103, 116, 216, 539, 701, 716, 1121
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 2 cut(s) 753, 805
VpaK11BI GGWCC 3 cut(s) 11, 1272, 1361
XapI RAATTY 2 cut(s) 358, 1282
XceI RCATGY 2 cut(s) 130, 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XspI CTAG 3 cut(s) 168, 1076, 1170
ZrmI AGTACT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.