FvH4_6g49270
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000641
Physical Location & Seq
Forward (+)
36956607 .. 36960583
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49270.t1

Sequence Viewer

Length: 1422 bp
ATGGGTCTGGTAAAGGTGGTGACTTGGAATGGGAGCAAATCAAAGGGAAGGAAGAAGAAGAAAGATGAGGCTTTGGAGGAAACTGGGTGTTGGGTTAAGTTCTCTTTTGGGACTTGCATGCCTTCTTCGAGATCCAAAGTTGATAGCACCATGACTGGGACTACTATGTCTTTTGCCGAAACTAAATCTTTGAATGAGAAAAGTAGAGATCGAGAGCTTACTCGTGGAGGTTCCTCTACAACTACTAGTAATGCTGGAAGTACTACATCCACACCCTATTTCAGTGAGGAGCTGAAGGTTGCTTCCCAGCTGCGGAAATTCACGTTTAATGAACTTAAGTTGGCAACTAGAAACTTCAGACCGGAGAGTCTTCTTGGTGAGGGTGGATTTGGTTGTGTGTTCAAGGGGTGGATAGAGGAAAATGGAACTGCGCCTGTGAAACCTGGTACTGGCCTTACAGTTGCAGTTAAGACCCTCAACCATGATGGACTTCAGGGTCACAAAGAATGGCTTGCTGAAATTTATTTTCTTGGTGATCTCATCCATCCAAATTTGGTAAAATTGATTGGTTACTCCATTGAAGATGATCAGAGGTTGCTGGTTTATGAGTTTTTACCACGAGGAAGTTTGGAAAACCACCTATTCAGAAGATCCCTGCCTCTTCCTTGGTCTATCAGAATGAAAATTGCACTTGGAGCCGCAAAGGGCCTTGCCTTTCTTCATGAAGAAGTTCAAAAACCAGTCATATACCGTGATTTCAAAACATCTAATATCCTATTAGACGCGGATTACAATGCCAAGCTCTCTGATTTTGGACTTGCTAAAGATGGTCCTGAAGGTGACAAGACTCATGTGTCTACACGAGTCATGGGAACATATGGTTATGCAGCCCCAGAATATGTCATGACTGGACATTTGACATCAAAGAGTGATGTCTACAGTTTTGGAGTAGTCCTACTTGAAATGCTAAATGGAAGAAGATCCATGGATAAAAATAGACCAAATGGGGAACACAATCTTGTGGAATGGGCAAGACAACTTTTTGGAGACAAGAGGAGGTTATTCCAATTAATTGACCCCCGTCTAGAAGGCCATTTTTCTATCAAGGGTGCGCAAAAAGCTATCCTACTGGCCGCTAGGTGCCTCAGTCGTGACCCAAAAGCCAGACCAATGATGAGTGAAGTTGTTGAAGCCCTAAAGCCTTTACCAAGCCTTAAGGACATGGCCATTTCTTCGTATCACTTTCAAACCATGCAAGCAGCTCGTACCAGATCCAACTTGAATGCTAAGAACGGCATTAGAACACAAGCGGTCTTTGTACAGAGGAATGGGCAACCGGTAAGGAGCTTGTCAAGCCCAAAGGGTCCTCCTGCTTCTCCATATTATCCTCAAAAATCACCAAAACCTAACTCAAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

474

Amino Acids

52.68

Weight (kDa)

9.67

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 122 - 397 8.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 123 - 399 2.9e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 166, 366, 495, 853
Acc16I TGCGCA 1 cut(s) 1113
AccB1I GGYRCC 1 cut(s) 1140
AccI GTMKAC 2 cut(s) 857, 936
AccII CGCG 1 cut(s) 785
AciI CCGC 5 cut(s) 313, 699, 785, 1134, 1310
AclWI GGATC 4 cut(s) 126, 645, 975, 1266
AcoI YGGCCR 2 cut(s) 1131, 1224
AcsI RAATTY 3 cut(s) 317, 519, 550
AcuI CTGAAG 4 cut(s) 314, 340, 476, 855
AfaI GTAC 4 cut(s) 262, 448, 1267, 1320
AfiI CCNNNNNNNGG 3 cut(s) 156, 312, 449
AflII CTTAAG 2 cut(s) 335, 1214
AgeI ACCGGT 1 cut(s) 1336
AgsI TTSAA 9 cut(s) 193, 403, 581, 734, 760, 962, 1190, 1247, 1282
AhlI ACTAGT 1 cut(s) 245
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 7 cut(s) 217, 292, 310, 802, 1121, 1262, 1347
AluI AGCT 7 cut(s) 217, 292, 310, 802, 1121, 1262, 1347
Alw26I GTCTC 1 cut(s) 1041
AlwI GGATC 4 cut(s) 126, 645, 975, 1266
AoxI GGCC 5 cut(s) 451, 706, 1090, 1131, 1224
ApeKI GCWGC 3 cut(s) 310, 887, 1259
ApoI RAATTY 3 cut(s) 317, 519, 550
ArsI GACNNNNNNTTYG 2 cut(s) 898, 930
AseI ATTAAT 1 cut(s) 1070
AsiGI ACCGGT 1 cut(s) 1336
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 2 cut(s) 433, 1114
AspS9I GGNCC 3 cut(s) 706, 830, 1364
AsuHPI GGTGA 5 cut(s) 31, 389, 545, 851, 1389
AvaII GGWCC 2 cut(s) 830, 1364
BalI TGGCCA 1 cut(s) 1226
BanI GGYRCC 1 cut(s) 1140
BauI CACGAG 3 cut(s) 222, 618, 861
BbsI GAAGAC 1 cut(s) 362
BbvI GCAGC 3 cut(s) 297, 899, 1271
BccI CCATC 3 cut(s) 479, 552, 821
BceAI ACGGC 1 cut(s) 1309
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BciT130I CCWGG 1 cut(s) 444
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 1041
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 4 cut(s) 246, 348, 1085, 1137
BfmI CTRYAG 1 cut(s) 937
BfrI CTTAAG 2 cut(s) 335, 1214
BisI GCNGC 5 cut(s) 311, 699, 888, 1134, 1260
BlsI GCNGC 5 cut(s) 312, 700, 889, 1135, 1261
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 444
Bme18I GGWCC 2 cut(s) 830, 1364
BmgT120I GGNCC 3 cut(s) 706, 830, 1364
BmiI GGNNCC 4 cut(s) 232, 697, 1142, 1365
BmrFI CCNGG 1 cut(s) 444
BmrI ACTGGG 2 cut(s) 93, 165
BmuI ACTGGG 2 cut(s) 93, 165
BoxI GACNNNNGTC 1 cut(s) 1080
BpiI GAAGAC 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 665, 984
BsaWI WCCGGW 2 cut(s) 361, 1336
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 312, 449
Bse118I RCCGGY 1 cut(s) 1336
Bse1I ACTGG 6 cut(s) 88, 160, 454, 740, 913, 1134
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 665, 984
BseGI GGATG 3 cut(s) 266, 540, 544
BseLI CCNNNNNNNGG 3 cut(s) 156, 312, 449
BseMII CTCAG 1 cut(s) 1159
BseNI ACTGG 6 cut(s) 88, 160, 454, 740, 913, 1134
BseRI GAGGAG 2 cut(s) 302, 1069
BseXI GCAGC 3 cut(s) 297, 899, 1271
BseYI CCCAGC 1 cut(s) 306
Bsh1236I CGCG 1 cut(s) 785
BshFI GGCC 5 cut(s) 453, 708, 1092, 1133, 1226
BshNI GGYRCC 1 cut(s) 1140
BshTI ACCGGT 1 cut(s) 1336
BsiSI CCGG 2 cut(s) 362, 1337
BslFI GGGAC 2 cut(s) 124, 172
BslI CCNNNNNNNGG 3 cut(s) 156, 312, 449
BsmAI GTCTC 1 cut(s) 1041
BsmFI GGGAC 2 cut(s) 124, 172
BsmI GAATGC 1 cut(s) 1288
BsnI GGCC 5 cut(s) 453, 708, 1092, 1133, 1226
Bsp1407I TGTACA 1 cut(s) 1318
Bsp143I GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
Bsp19I CCATGG 1 cut(s) 984
BspACI CCGC 5 cut(s) 313, 699, 785, 1134, 1310
BspANI GGCC 5 cut(s) 453, 708, 1092, 1133, 1226
BspCNI CTCAG 1 cut(s) 1158
BspFNI CGCG 1 cut(s) 785
BspHI TCATGA 2 cut(s) 721, 903
BspLI GGNNCC 4 cut(s) 232, 697, 1142, 1365
BspPI GGATC 4 cut(s) 126, 645, 975, 1266
BspT107I GGYRCC 1 cut(s) 1140
BspTI CTTAAG 2 cut(s) 335, 1214
BsrFI RCCGGY 1 cut(s) 1336
BsrGI TGTACA 1 cut(s) 1318
BsrI ACTGG 6 cut(s) 88, 160, 454, 740, 913, 1134
BssAI RCCGGY 1 cut(s) 1336
BssECI CCNNGG 2 cut(s) 665, 984
BssMI GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
BssSI CACGAG 3 cut(s) 222, 618, 861
BssT1I CCWWGG 2 cut(s) 665, 984
Bst2BI CACGAG 3 cut(s) 222, 618, 861
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 3 cut(s) 460, 752, 941
Bst6I CTCTTC 1 cut(s) 666
BstAFI CTTAAG 2 cut(s) 335, 1214
BstAUI TGTACA 1 cut(s) 1318
BstC8I GCNNGC 3 cut(s) 119, 513, 1257
BstDEI CTNAG 2 cut(s) 1145, 1287
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 3 cut(s) 266, 540, 544
BstFNI CGCG 1 cut(s) 785
BstHHI GCGC 2 cut(s) 433, 1114
BstKTI GATC 7 cut(s) 134, 211, 538, 589, 653, 983, 1274
BstMAI GTCTC 1 cut(s) 1041
BstMBI GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
BstMWI GCNNNNNNNGC 3 cut(s) 695, 1118, 1353
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 1 cut(s) 121
BstPAI GACNNNNGTC 1 cut(s) 1080
BstSCI CCNGG 1 cut(s) 442
BstSFI CTRYAG 1 cut(s) 937
BstUI CGCG 1 cut(s) 785
BstV1I GCAGC 3 cut(s) 297, 899, 1271
BstV2I GAAGAC 1 cut(s) 362
BstX2I RGATCY 4 cut(s) 131, 650, 980, 1271
BstYI RGATCY 4 cut(s) 131, 650, 980, 1271
BsuRI GGCC 5 cut(s) 453, 708, 1092, 1133, 1226
BtgI CCRYGG 1 cut(s) 984
BtsCI GGATG 3 cut(s) 266, 540, 544
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 119, 513, 1257
CciI TCATGA 2 cut(s) 721, 903
CfoI GCGC 2 cut(s) 433, 1114
Cfr10I RCCGGY 1 cut(s) 1336
Cfr13I GGNCC 3 cut(s) 706, 830, 1364
CseI GACGC 1 cut(s) 791
CsiI ACCWGGT 1 cut(s) 442
Csp6I GTAC 4 cut(s) 261, 447, 1266, 1319
CspAI ACCGGT 1 cut(s) 1336
CviQI GTAC 4 cut(s) 261, 447, 1266, 1319
DdeI CTNAG 2 cut(s) 1145, 1287
DpnI GATC 7 cut(s) 133, 210, 537, 588, 652, 982, 1273
DpnII GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
DrdI GACNNNNNNGTC 4 cut(s) 166, 366, 495, 853
DseDI GACNNNNNNGTC 4 cut(s) 166, 366, 495, 853
EaeI YGGCCR 2 cut(s) 1131, 1224
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
Eco130I CCWWGG 2 cut(s) 665, 984
Eco47I GGWCC 2 cut(s) 830, 1364
Eco57I CTGAAG 4 cut(s) 314, 340, 476, 855
EcoO109I RGGNCCY 2 cut(s) 706, 1364
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 665, 984
ErhI CCWWGG 2 cut(s) 665, 984
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 2 cut(s) 124, 172
FauNDI CATATG 1 cut(s) 877
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 2 cut(s) 857, 936
Fnu4HI GCNGC 5 cut(s) 311, 699, 888, 1134, 1260
FokI GGATG 3 cut(s) 253, 527, 531
Fsp4HI GCNGC 5 cut(s) 311, 699, 888, 1134, 1260
FspBI CTAG 4 cut(s) 246, 348, 1085, 1137
FspI TGCGCA 1 cut(s) 1113
GlaI GCGC 2 cut(s) 432, 1113
GluI GCNGC 5 cut(s) 311, 699, 888, 1134, 1260
GsaI CCCAGC 1 cut(s) 310
HaeIII GGCC 5 cut(s) 453, 708, 1092, 1133, 1226
HapII CCGG 2 cut(s) 362, 1337
HgaI GACGC 1 cut(s) 791
HhaI GCGC 2 cut(s) 433, 1114
Hin6I GCGC 2 cut(s) 431, 1112
HinP1I GCGC 2 cut(s) 431, 1112
HinfI GANTC 3 cut(s) 367, 847, 864
HpaII CCGG 2 cut(s) 362, 1337
HphI GGTGA 5 cut(s) 31, 389, 545, 851, 1389
Hpy166II GTNNAC 2 cut(s) 858, 937
Hpy188I TCNGA 5 cut(s) 359, 591, 647, 677, 808
Hpy188III TCNNGA 7 cut(s) 129, 212, 722, 833, 904, 1085, 1151
Hpy8I GTNNAC 2 cut(s) 858, 937
HpyAV CCTTC 5 cut(s) 42, 132, 289, 830, 1082
HpyCH4III ACNGT 3 cut(s) 460, 752, 941
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 5 cut(s) 117, 464, 689, 887, 1255
HpyF10VI GCNNNNNNNGC 3 cut(s) 695, 1118, 1353
HpyF3I CTNAG 2 cut(s) 1145, 1287
HpySE526I ACGT 1 cut(s) 323
HspAI GCGC 2 cut(s) 431, 1112
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
LmnI GCTCC 4 cut(s) 33, 289, 695, 1344
Lsp1109I GCAGC 3 cut(s) 297, 899, 1271
MabI ACCWGGT 1 cut(s) 442
MaeI CTAG 4 cut(s) 246, 348, 1085, 1137
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 5 cut(s) 19, 497, 569, 839, 1151
MalI GATC 7 cut(s) 133, 210, 537, 588, 652, 982, 1273
MboI GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
MflI RGATCY 4 cut(s) 131, 650, 980, 1271
MlsI TGGCCA 1 cut(s) 1226
MluCI AATT 7 cut(s) 317, 519, 550, 560, 684, 1067, 1071
MluNI TGGCCA 1 cut(s) 1226
MlyI GAGTC 3 cut(s) 376, 841, 873
MmeI TCCRAC 1 cut(s) 1299
Mox20I TGGCCA 1 cut(s) 1226
MroXI GAANNNNTTC 1 cut(s) 729
MscI TGGCCA 1 cut(s) 1226
MseI TTAA 6 cut(s) 96, 327, 336, 468, 1070, 1215
Msp20I TGGCCA 1 cut(s) 1226
MspA1I CMGCKG 1 cut(s) 310
MspCI CTTAAG 2 cut(s) 335, 1214
MspI CCGG 2 cut(s) 362, 1337
MspR9I CCNGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 1 cut(s) 444
MvnI CGCG 1 cut(s) 785
MwoI GCNNNNNNNGC 3 cut(s) 695, 1118, 1353
NcoI CCATGG 1 cut(s) 984
NdeI CATATG 1 cut(s) 877
NdeII GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
NlaIV GGNNCC 4 cut(s) 232, 697, 1142, 1365
NmuCI GTSAC 4 cut(s) 19, 497, 839, 1151
NsbI TGCGCA 1 cut(s) 1113
NspI RCATGY 1 cut(s) 121
PaeI GCATGC 1 cut(s) 121
PagI TCATGA 2 cut(s) 721, 903
PctI GAATGC 1 cut(s) 1288
PdmI GAANNNNTTC 1 cut(s) 729
PinAI ACCGGT 1 cut(s) 1336
PkrI GCNGC 5 cut(s) 312, 700, 889, 1135, 1261
PleI GAGTC 3 cut(s) 375, 841, 872
PpsI GAGTC 3 cut(s) 375, 841, 872
PpuMI RGGWCCY 1 cut(s) 1364
PshAI GACNNNNGTC 1 cut(s) 1080
PshBI ATTAAT 1 cut(s) 1070
Psp5II RGGWCCY 1 cut(s) 1364
Psp6I CCWGG 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 4 cut(s) 232, 697, 1142, 1365
PspPI GGNCC 3 cut(s) 706, 830, 1364
PspPPI RGGWCCY 1 cut(s) 1364
PsuI RGATCY 4 cut(s) 131, 650, 980, 1271
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 4 cut(s) 262, 448, 1267, 1320
RsaNI GTAC 4 cut(s) 261, 447, 1266, 1319
SaqAI TTAA 6 cut(s) 96, 327, 336, 468, 1070, 1215
SatI GCNGC 5 cut(s) 311, 699, 888, 1134, 1260
Sau3AI GATC 7 cut(s) 131, 208, 535, 586, 650, 980, 1271
Sau96I GGNCC 3 cut(s) 706, 830, 1364
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 3 cut(s) 376, 841, 873
ScrFI CCNGG 1 cut(s) 444
SexAI ACCWGGT 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 937
SinI GGWCC 2 cut(s) 830, 1364
SmlI CTYRAG 2 cut(s) 335, 1214
SmoI CTYRAG 2 cut(s) 335, 1214
SpeI ACTAGT 1 cut(s) 245
SphI GCATGC 1 cut(s) 121
Sse9I AATT 7 cut(s) 317, 519, 550, 560, 684, 1067, 1071
SsiI CCGC 5 cut(s) 313, 699, 785, 1134, 1310
SspMI CTAG 4 cut(s) 246, 348, 1085, 1137
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 2 cut(s) 665, 984
TaaI ACNGT 3 cut(s) 460, 752, 941
TaiI ACGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 128, 211
TasI AATT 7 cut(s) 317, 519, 550, 560, 684, 1067, 1071
TatI WGTACW 2 cut(s) 260, 1318
TauI GCSGC 2 cut(s) 701, 1136
Tru1I TTAA 6 cut(s) 96, 327, 336, 468, 1070, 1215
Tru9I TTAA 6 cut(s) 96, 327, 336, 468, 1070, 1215
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 4 cut(s) 19, 497, 839, 1151
TseI GCWGC 3 cut(s) 310, 887, 1259
Tsp45I GTSAC 4 cut(s) 19, 497, 839, 1151
TspDTI ATGAA 4 cut(s) 345, 695, 710, 738
TspRI CASTG 1 cut(s) 289
Vha464I CTTAAG 2 cut(s) 335, 1214
VpaK11BI GGWCC 2 cut(s) 830, 1364
VspI ATTAAT 1 cut(s) 1070
XapI RAATTY 3 cut(s) 317, 519, 550
XbaI TCTAGA 1 cut(s) 1084
XceI RCATGY 1 cut(s) 121
XmiI GTMKAC 2 cut(s) 857, 936
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 4 cut(s) 246, 348, 1085, 1137
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.