MD16G1078600.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000641
Physical Location & Seq
Reverse (-)
5515515 .. 5519550
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1078600.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGGGTTTGAAAGCTGAGGATGGTAAAGTGGAGGCCTGGGACATCTGCAAATCGAAGGTGAGGAGGAAGAAGAAGCAGAAAGCTGATGGGGATGATGATGATGACGTGGAGGAGGAGGGGAGTGGGTGTTGGGTTAGGCTGAGGTACTTTGGGAGCTGCATTTCTTCAAGATCCAAAGTTGATAGCTCAATCAGTGGGACCAGTAATACTCACTGTGGTGAAACTAAATCTATAAATGATACGAGCAGGGACCAACCAACAGCTACTGTAGTCTCTTCATCAACCACTAGTAATACAGAAAGTAATGCATCCACTTCCAAACTTGAAGAGGAACTTAAAGTTGCTTCACGGTTGCGAAAATTCACATTTATTGATCTTAAATGGGCAACAAGAAATTTCAGACCTGAAAGTCTTCTTGGTGAAGGTGGTTTTGGTTGTGTGTTCAAGGGGTGGATAGAAGAAAATGGAACTGCTCCAGTAAAACCTGGCACAGGTCTTACTGTTGCCGTTAAAACTCTTAACCATGATGGACTTCAGGGTCATAAAGAATGGCTGGCTGAAGTAAATTATCTTGGTGACCTTGTCCATCCAAACTTAGTTAAACTTGTAGGATACTGCATTGAAGATGATCAAAGGCTGCTAGTGTATGAGTTCATGCCTCGAGGGAGCTTGGAGAACCATCTATTTAGGAGGTCGTTGCCTCTTCCATGGTCTATTCGAATGAAGATTGCATTAGGTGCTGCAAAAGGCCTTGCTTTTCTCCACGAGGAAGCAGAAAGACCTGTGATCTATCGTGACTTTAAAACCTCCAACATTTTATTGGACGCCGATTATAATGCCAAGCTGTCTGACTTCGGACTTGCCAAAGATGGTCCCGAGGGTGATAAAACTCATGTCTCCACCCGTGTGATGGGAACTTATGGTTATGCAGCTCCTGAATATGTAATGACTGGACATCTTACATCCAGGAGTGATGTATATAGTTTTGGCGTGGTTTTACTCGAGATGCTGACCGGCAGAAGATCCATGGACAAAAACCGACCCAATGGCGAACATAACCTTGTGGAATGGGCACGGCCACATCTAGGAGAAAGAAGAAGATTCTACCGGTTGATAGACCCTCGGCTTGAAGGCCACTTTTCGATAAAAGGTGTCCAAAAAGCCGCACAACTAGCTGCCCACTGCCTTAGTCGAGATCCAAAAGCCAGGCCCCTAATGAGTGAAGTTGTGGAAGCCTTAAAGCCCCTGCCAGCCCTCAAGGATATGGCAAGCTCATCATATTATTTCCAGACAATGCAAGGTGACCGTGTCGGTTCCAGCCCAAATACCAGAAACGGCGTCAGACCACAAGGAGGATTGCTGACGAGGAACGGGCATCATCAGCAGAGGAGCCTTTCGATACCAAATGGTACCCATGCTTCTCCATATCACCATCAATATCCCCATCCATCGCCAAAACCTAATGTCAAACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

492

Amino Acids

54.71

Weight (kDa)

9.01

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 136 - 411 2.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 137 - 413 9.1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AasI GACNNNNNNGTC 2 cut(s) 408, 537
AbsI CCTCGAGG 1 cut(s) 660
Acc65I GGTACC 1 cut(s) 1409
AccB1I GGYRCC 1 cut(s) 1409
AciI CCGC 1 cut(s) 1164
AclWI GGATC 3 cut(s) 165, 1017, 1190
AcoI YGGCCR 1 cut(s) 1076
AcsI RAATTY 2 cut(s) 359, 394
AcuI CTGAAG 2 cut(s) 518, 579
AcyI GRCGYC 2 cut(s) 825, 1338
AfaI GTAC 2 cut(s) 146, 1411
AfiI CCNNNNNNNGG 4 cut(s) 491, 910, 1085, 1352
AgeI ACCGGT 1 cut(s) 1107
AgsI TTSAA 6 cut(s) 10, 168, 326, 445, 623, 1130
AhlI ACTAGT 1 cut(s) 287
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 4 cut(s) 35, 484, 965, 1205
AjuI GAANNNNNNNTTGG 4 cut(s) 399, 414, 431, 446
AleI CACNNNNGTG 2 cut(s) 216, 905
Alw26I GTCTC 2 cut(s) 277, 901
AlwI GGATC 3 cut(s) 165, 1017, 1190
AlwNI CAGNNNCTG 2 cut(s) 266, 935
Ama87I CYCGRG 3 cut(s) 660, 875, 1001
AoxI GGCC 5 cut(s) 33, 748, 1076, 1132, 1208
ApeKI GCWGC 5 cut(s) 156, 637, 740, 929, 1175
ApoI RAATTY 2 cut(s) 359, 394
AsiGI ACCGGT 1 cut(s) 1107
Asp700I GAANNNNTTC 1 cut(s) 411
Asp718I GGTACC 1 cut(s) 1409
AspS9I GGNCC 4 cut(s) 198, 250, 872, 1209
AsuHPI GGTGA 7 cut(s) 70, 230, 431, 587, 893, 1313, 1421
AsuII TTCGAA 1 cut(s) 718
AvaI CYCGRG 3 cut(s) 660, 875, 1001
AvaII GGWCC 3 cut(s) 198, 250, 872
BaeGI GKGCMC 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 1409
BarI GAAGNNNNNNTAC 2 cut(s) 1402, 1434
BauI CACGAG 1 cut(s) 764
BbsI GAAGAC 1 cut(s) 404
BbvCI CCTCAGC 2 cut(s) 15, 140
BbvI GCAGC 5 cut(s) 143, 624, 727, 941, 1162
BceAI ACGGC 3 cut(s) 491, 1091, 1351
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BciT130I CCWGG 4 cut(s) 37, 486, 967, 1207
BciVI GTATCC 1 cut(s) 605
BclI TGATCA 1 cut(s) 628
BcoDI GTCTC 2 cut(s) 277, 901
BcuI ACTAGT 1 cut(s) 287
BfaI CTAG 4 cut(s) 288, 641, 1085, 1172
BfmI CTRYAG 1 cut(s) 267
BfuI GTATCC 1 cut(s) 605
BisI GCNGC 6 cut(s) 157, 638, 741, 930, 1164, 1176
BlsI GCNGC 6 cut(s) 158, 639, 742, 931, 1165, 1177
Bme1390I CCNGG 4 cut(s) 37, 486, 967, 1207
Bme18I GGWCC 3 cut(s) 198, 250, 872
BmeT110I CYCGRG 3 cut(s) 660, 875, 1001
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 198, 250, 872, 1209
BmiI GGNNCC 7 cut(s) 199, 251, 874, 1211, 1315, 1391, 1411
BmrFI CCNGG 4 cut(s) 37, 486, 967, 1207
BmsI GCATC 3 cut(s) 317, 996, 1384
BpiI GAAGAC 1 cut(s) 404
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 2 cut(s) 15, 140
Bpu14I TTCGAA 1 cut(s) 718
BpuEI CTTGAG 1 cut(s) 1241
BsaHI GRCGYC 2 cut(s) 825, 1338
BsaJI CCNNGG 5 cut(s) 36, 707, 876, 1026, 1121
BsaWI WCCGGW 1 cut(s) 1107
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 4 cut(s) 491, 910, 1085, 1352
Bse118I RCCGGY 2 cut(s) 1013, 1107
Bse1I ACTGG 3 cut(s) 201, 476, 955
BseBI CCWGG 4 cut(s) 37, 486, 967, 1207
BseDI CCNNGG 5 cut(s) 36, 707, 876, 1026, 1121
BseGI GGATG 6 cut(s) 25, 97, 308, 586, 962, 1444
BseLI CCNNNNNNNGG 4 cut(s) 491, 910, 1085, 1352
BseMII CTCAG 2 cut(s) 6, 131
BseNI ACTGG 3 cut(s) 201, 476, 955
BseRI GAGGAG 4 cut(s) 76, 125, 128, 1402
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 5 cut(s) 143, 624, 727, 941, 1162
BshFI GGCC 5 cut(s) 35, 750, 1078, 1134, 1210
BshNI GGYRCC 1 cut(s) 1409
BshTI ACCGGT 1 cut(s) 1107
BsiHKCI CYCGRG 3 cut(s) 660, 875, 1001
BsiSI CCGG 2 cut(s) 1014, 1108
BslFI GGGAC 4 cut(s) 53, 211, 263, 858
BslI CCNNNNNNNGG 4 cut(s) 491, 910, 1085, 1352
BsmAI GTCTC 2 cut(s) 277, 901
BsmFI GGGAC 4 cut(s) 53, 211, 263, 858
BsnI GGCC 5 cut(s) 35, 750, 1078, 1134, 1210
BsoBI CYCGRG 3 cut(s) 660, 875, 1001
Bsp119I TTCGAA 1 cut(s) 718
Bsp1286I GDGCHC 1 cut(s) 1075
Bsp143I GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
Bsp19I CCATGG 2 cut(s) 707, 1026
BspACI CCGC 1 cut(s) 1164
BspANI GGCC 5 cut(s) 35, 750, 1078, 1134, 1210
BspCNI CTCAG 2 cut(s) 7, 132
BspLI GGNNCC 7 cut(s) 199, 251, 874, 1211, 1315, 1391, 1411
BspPI GGATC 3 cut(s) 165, 1017, 1190
BspT104I TTCGAA 1 cut(s) 718
BspT107I GGYRCC 1 cut(s) 1409
BsrFI RCCGGY 2 cut(s) 1013, 1107
BsrI ACTGG 3 cut(s) 201, 476, 955
BssAI RCCGGY 2 cut(s) 1013, 1107
BssECI CCNNGG 5 cut(s) 36, 707, 876, 1026, 1121
BssMI GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
BssNI GRCGYC 2 cut(s) 825, 1338
BssSI CACGAG 1 cut(s) 764
BssT1I CCWWGG 2 cut(s) 707, 1026
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 4 cut(s) 37, 486, 967, 1207
Bst4CI ACNGT 6 cut(s) 215, 268, 351, 502, 1307, 1473
Bst6I CTCTTC 3 cut(s) 280, 321, 708
BstACI GRCGYC 2 cut(s) 825, 1338
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 718
BstC8I GCNNGC 3 cut(s) 555, 1251, 1270
BstDEI CTNAG 4 cut(s) 15, 140, 595, 1187
BstDSI CCRYGG 2 cut(s) 707, 1026
BstEII GGTNACC 2 cut(s) 575, 1301
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 6 cut(s) 25, 97, 308, 586, 962, 1444
BstKTI GATC 6 cut(s) 173, 376, 631, 789, 1025, 1198
BstMAI GTCTC 2 cut(s) 277, 901
BstMBI GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
BstMWI GCNNNNNNNGC 3 cut(s) 737, 1172, 1381
BstNI CCWGG 4 cut(s) 37, 486, 967, 1207
BstPI GGTNACC 2 cut(s) 575, 1301
BstSCI CCNGG 4 cut(s) 35, 484, 965, 1205
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 1075
BstV1I GCAGC 5 cut(s) 143, 624, 727, 941, 1162
BstV2I GAAGAC 1 cut(s) 404
BstX2I RGATCY 3 cut(s) 170, 1022, 1195
BstYI RGATCY 3 cut(s) 170, 1022, 1195
BsuI GTATCC 1 cut(s) 605
BsuRI GGCC 5 cut(s) 35, 750, 1078, 1134, 1210
BtgI CCRYGG 2 cut(s) 707, 1026
BtgZI GCGATG 1 cut(s) 1434
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 6 cut(s) 25, 97, 308, 586, 962, 1444
BtsI GCAGTG 1 cut(s) 1180
BtsIMutI CAGTG 3 cut(s) 199, 211, 1180
Cac8I GCNNGC 3 cut(s) 555, 1251, 1270
CaiI CAGNNNCTG 2 cut(s) 266, 935
Cfr10I RCCGGY 2 cut(s) 1013, 1107
Cfr13I GGNCC 4 cut(s) 198, 250, 872, 1209
CseI GACGC 2 cut(s) 833, 1327
Csp6I GTAC 2 cut(s) 145, 1410
CspAI ACCGGT 1 cut(s) 1107
CviAII CATG 6 cut(s) 524, 655, 708, 893, 1027, 1415
CviQI GTAC 2 cut(s) 145, 1410
DdeI CTNAG 4 cut(s) 15, 140, 595, 1187
DpnI GATC 6 cut(s) 172, 375, 630, 788, 1024, 1197
DpnII GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
DraI TTTAAA 1 cut(s) 802
DrdI GACNNNNNNGTC 2 cut(s) 408, 537
DseDI GACNNNNNNGTC 2 cut(s) 408, 537
EaeI YGGCCR 1 cut(s) 1076
Eam1104I CTCTTC 3 cut(s) 280, 321, 708
EarI CTCTTC 3 cut(s) 280, 321, 708
Eco130I CCWWGG 2 cut(s) 707, 1026
Eco147I AGGCCT 2 cut(s) 35, 750
Eco47I GGWCC 3 cut(s) 198, 250, 872
Eco57I CTGAAG 2 cut(s) 518, 579
Eco88I CYCGRG 3 cut(s) 660, 875, 1001
Eco91I GGTNACC 2 cut(s) 575, 1301
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 1209
EcoO65I GGTNACC 2 cut(s) 575, 1301
EcoRII CCWGG 4 cut(s) 35, 484, 965, 1205
EcoT14I CCWWGG 2 cut(s) 707, 1026
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 2 cut(s) 707, 1026
FaeI CATG 6 cut(s) 527, 658, 711, 896, 1030, 1418
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 4 cut(s) 53, 211, 263, 858
FatI CATG 6 cut(s) 523, 654, 707, 892, 1026, 1414
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 6 cut(s) 157, 638, 741, 930, 1164, 1176
FokI GGATG 6 cut(s) 32, 104, 295, 573, 949, 1431
Fsp4HI GCNGC 6 cut(s) 157, 638, 741, 930, 1164, 1176
FspBI CTAG 4 cut(s) 288, 641, 1085, 1172
GluI GCNGC 6 cut(s) 157, 638, 741, 930, 1164, 1176
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 5 cut(s) 35, 750, 1078, 1134, 1210
HapII CCGG 2 cut(s) 1014, 1108
HgaI GACGC 2 cut(s) 833, 1327
Hin1I GRCGYC 2 cut(s) 825, 1338
Hin1II CATG 6 cut(s) 527, 658, 711, 896, 1030, 1418
HinfI GANTC 1 cut(s) 1101
HpaII CCGG 2 cut(s) 1014, 1108
HphI GGTGA 7 cut(s) 70, 230, 431, 587, 893, 1313, 1421
Hpy188I TCNGA 4 cut(s) 401, 850, 857, 1343
Hpy188III TCNNGA 7 cut(s) 168, 794, 875, 935, 1003, 1193, 1288
HpyAV CCTTC 3 cut(s) 49, 416, 1124
HpyCH4III ACNGT 6 cut(s) 215, 268, 351, 502, 1307, 1473
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 8 cut(s) 48, 159, 308, 618, 731, 743, 929, 1297
HpyF10VI GCNNNNNNNGC 3 cut(s) 737, 1172, 1381
HpyF3I CTNAG 4 cut(s) 15, 140, 595, 1187
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 2 cut(s) 825, 1338
Hsp92II CATG 6 cut(s) 527, 658, 711, 896, 1030, 1418
KpnI GGTACC 1 cut(s) 1413
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
LmnI GCTCC 5 cut(s) 153, 478, 666, 937, 1389
Lsp1109I GCAGC 5 cut(s) 143, 624, 727, 941, 1162
LweI GCATC 3 cut(s) 317, 996, 1384
MaeI CTAG 4 cut(s) 288, 641, 1085, 1172
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 575, 794, 1301
MalI GATC 6 cut(s) 172, 375, 630, 788, 1024, 1197
MboI GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
MflI RGATCY 3 cut(s) 170, 1022, 1195
MhlI GDGCHC 1 cut(s) 1075
MluCI AATT 3 cut(s) 359, 394, 565
MmeI TCCRAC 1 cut(s) 834
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 7 cut(s) 336, 378, 510, 519, 600, 801, 1238
MslI CAYNNNNRTG 2 cut(s) 216, 905
MspI CCGG 2 cut(s) 1014, 1108
MspR9I CCNGG 4 cut(s) 37, 486, 967, 1207
MvaI CCWGG 4 cut(s) 37, 486, 967, 1207
MwoI GCNNNNNNNGC 3 cut(s) 737, 1172, 1381
NcoI CCATGG 2 cut(s) 707, 1026
NdeII GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
NlaIII CATG 6 cut(s) 527, 658, 711, 896, 1030, 1418
NlaIV GGNNCC 7 cut(s) 199, 251, 874, 1211, 1315, 1391, 1411
NmeAIII GCCGAG 1 cut(s) 1102
NmuCI GTSAC 3 cut(s) 575, 794, 1301
NsiI ATGCAT 1 cut(s) 310
NspV TTCGAA 1 cut(s) 718
OliI CACNNNNGTG 2 cut(s) 216, 905
PaeR7I CTCGAG 2 cut(s) 660, 1001
PceI AGGCCT 2 cut(s) 35, 750
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 1 cut(s) 1101
PflFI GACNNNGTC 2 cut(s) 581, 1307
PfoI TCCNGGA 1 cut(s) 965
PinAI ACCGGT 1 cut(s) 1107
PkrI GCNGC 6 cut(s) 158, 639, 742, 931, 1165, 1177
PsiI TTATAA 1 cut(s) 834
Psp6I CCWGG 4 cut(s) 35, 484, 965, 1205
PspEI GGTNACC 2 cut(s) 575, 1301
PspGI CCWGG 4 cut(s) 35, 484, 965, 1205
PspN4I GGNNCC 7 cut(s) 199, 251, 874, 1211, 1315, 1391, 1411
PspPI GGNCC 4 cut(s) 198, 250, 872, 1209
PspXI VCTCGAGB 1 cut(s) 660
PstNI CAGNNNCTG 2 cut(s) 266, 935
PsuI RGATCY 3 cut(s) 170, 1022, 1195
PsyI GACNNNGTC 2 cut(s) 581, 1307
RsaI GTAC 2 cut(s) 146, 1411
RsaNI GTAC 2 cut(s) 145, 1410
RseI CAYNNNNRTG 2 cut(s) 216, 905
SaqAI TTAA 7 cut(s) 336, 378, 510, 519, 600, 801, 1238
SatI GCNGC 6 cut(s) 157, 638, 741, 930, 1164, 1176
Sau3AI GATC 6 cut(s) 170, 373, 628, 786, 1022, 1195
Sau96I GGNCC 4 cut(s) 198, 250, 872, 1209
ScrFI CCNGG 4 cut(s) 37, 486, 967, 1207
SduI GDGCHC 1 cut(s) 1075
SfaNI GCATC 3 cut(s) 317, 996, 1384
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 2 cut(s) 660, 1001
SfuI TTCGAA 1 cut(s) 718
SinI GGWCC 3 cut(s) 198, 250, 872
SlaI CTCGAG 2 cut(s) 660, 1001
SmiMI CAYNNNNRTG 2 cut(s) 216, 905
SmlI CTYRAG 3 cut(s) 660, 1001, 1256
SmoI CTYRAG 3 cut(s) 660, 1001, 1256
SpeI ACTAGT 1 cut(s) 287
Sse9I AATT 3 cut(s) 359, 394, 565
SseBI AGGCCT 2 cut(s) 35, 750
SsiI CCGC 1 cut(s) 1164
SspMI CTAG 4 cut(s) 288, 641, 1085, 1172
StuI AGGCCT 2 cut(s) 35, 750
StyD4I CCNGG 4 cut(s) 35, 484, 965, 1205
StyI CCWWGG 2 cut(s) 707, 1026
TaaI ACNGT 6 cut(s) 215, 268, 351, 502, 1307, 1473
TaiI ACGT 1 cut(s) 108
TaqI TCGA 7 cut(s) 53, 661, 718, 1002, 1142, 1192, 1397
TasI AATT 3 cut(s) 359, 394, 565
TauI GCSGC 1 cut(s) 1166
TfiI GAWTC 1 cut(s) 1101
Tru1I TTAA 7 cut(s) 336, 378, 510, 519, 600, 801, 1238
Tru9I TTAA 7 cut(s) 336, 378, 510, 519, 600, 801, 1238
TscAI CASTG 3 cut(s) 199, 218, 1187
TseFI GTSAC 3 cut(s) 575, 794, 1301
TseI GCWGC 5 cut(s) 156, 637, 740, 929, 1175
Tsp45I GTSAC 3 cut(s) 575, 794, 1301
TspDTI ATGAA 3 cut(s) 267, 643, 737
TspRI CASTG 3 cut(s) 199, 218, 1187
Tth111I GACNNNGTC 2 cut(s) 581, 1307
VpaK11BI GGWCC 3 cut(s) 198, 250, 872
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 2 cut(s) 359, 394
XcmI CCANNNNNNNNNTGG 2 cut(s) 817, 907
XhoI CTCGAG 2 cut(s) 660, 1001
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 4 cut(s) 288, 641, 1085, 1172
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.