Rroxscaffold_2G00082390
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000641
Physical Location & Seq
Forward (+)
5242280 .. 5246263
3984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082390.1

Sequence Viewer

Length: 744 bp
ATGAAAATTGCACTTGGTGCTGCAAAGGGTCTTGCCTTTCTTCATGAAGAAGTTCAAAAACCTGTCATATACCGTGACTTCAAAACATCTAATATCCTATTAGACGCGGATTACAACGCCAAGCTCTCGGATTTTGGACTTGCCAAAGATGGTCCTGAAGGTGATAAGACTCATGTGTCTACGCGAGTCATGGGAACCTATGGTTATGCAGCCCCAGAATATGTGATGACTGGACATTTGACATCAAAGAGTGATGTCTACAGTTTTGGAGTAGTCCTACTCGAAATGCTAAATGGAAGAAGATCCATGGATAAAAATAGACCAAATGGGGAACACAATCTTGTGGAATGGGCGAGACAACTTTTTGGAGACAAGAGGAGGTTATTCCAGTTAATTGACCCCCGGCTCGAAGGCCATTTTTCAATCAAGGGTGCACAAAAAGCTATCCAACTGGCTGCCCGCTGCCTCAGCCGTGACCCAAAATCCAGACCAATGATGAGTGAAGTTGTTGAAGCCCTAAAGCCTTTACCAAGCCTCAAGGACATGGCCAGTTCTTCTTATCACTTTCAAACCATGCAAGCAGCTCGTACCAGATCCAACTTGAATGCTAAGAATGGCATTAGAACACAAGCAATCTTTGTACCGAGGAATGGACAACCGGTAAGGAGCTTGTCAAGCCCAAAGGGTCCTCCTGCTTCTCCATGTTATCCTCAGAAATCACCTAAACCTAACTCGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

247

Amino Acids

27.43

Weight (kDa)

9.82

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 2 - 173 2.1e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 3 - 171 7.4e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 175
AccI GTMKAC 2 cut(s) 179, 258
AccII CGCG 2 cut(s) 107, 184
AciI CCGC 2 cut(s) 107, 460
AclWI GGATC 2 cut(s) 297, 588
AcoI YGGCCR 1 cut(s) 546
AcuI CTGAAG 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 17
AfaI GTAC 2 cut(s) 589, 642
AfiI CCNNNNNNNGG 1 cut(s) 650
AgeI ACCGGT 1 cut(s) 658
AgsI TTSAA 6 cut(s) 56, 82, 423, 512, 569, 604
AluBI AGCT 4 cut(s) 124, 443, 584, 669
AluI AGCT 4 cut(s) 124, 443, 584, 669
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 349, 363
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 2 cut(s) 297, 588
AoxI GGCC 2 cut(s) 412, 546
ApaLI GTGCAC 1 cut(s) 432
ApeKI GCWGC 5 cut(s) 20, 209, 455, 462, 581
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
AsiGI ACCGGT 1 cut(s) 658
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 152, 686
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 173, 711
AvaII GGWCC 2 cut(s) 152, 686
BaeGI GKGCMC 1 cut(s) 436
BalI TGGCCA 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 467
BbvI GCAGC 5 cut(s) 7, 221, 442, 449, 593
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 456
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 349, 363
BfmI CTRYAG 1 cut(s) 259
BisI GCNGC 5 cut(s) 21, 210, 456, 463, 582
BlsI GCNGC 5 cut(s) 22, 211, 457, 464, 583
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 2 cut(s) 152, 686
BmgT120I GGNCC 2 cut(s) 152, 686
BmiI GGNNCC 2 cut(s) 196, 687
BmrFI CCNGG 1 cut(s) 403
Bpu10I CCTNAGC 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 403
BsaJI CCNNGG 3 cut(s) 306, 401, 644
BsaWI WCCGGW 1 cut(s) 658
Bsc4I CCNNNNNNNGG 1 cut(s) 650
Bse118I RCCGGY 1 cut(s) 658
Bse1I ACTGG 4 cut(s) 235, 388, 456, 549
BseDI CCNNGG 3 cut(s) 306, 401, 644
BseLI CCNNNNNNNGG 1 cut(s) 650
BseMII CTCAG 2 cut(s) 481, 725
BseNI ACTGG 4 cut(s) 235, 388, 456, 549
BseRI GAGGAG 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 5 cut(s) 7, 221, 442, 449, 593
Bsh1236I CGCG 2 cut(s) 107, 184
BshFI GGCC 2 cut(s) 414, 548
BshTI ACCGGT 1 cut(s) 658
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 2 cut(s) 403, 659
BslI CCNNNNNNNGG 1 cut(s) 650
BsmAI GTCTC 2 cut(s) 349, 363
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 2 cut(s) 414, 548
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 2 cut(s) 302, 593
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 2 cut(s) 107, 460
BspANI GGCC 2 cut(s) 414, 548
BspCNI CTCAG 2 cut(s) 480, 724
BspFNI CGCG 2 cut(s) 107, 184
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 196, 687
BspPI GGATC 2 cut(s) 297, 588
BsrFI RCCGGY 1 cut(s) 658
BsrI ACTGG 4 cut(s) 235, 388, 456, 549
BssAI RCCGGY 1 cut(s) 658
BssECI CCNNGG 3 cut(s) 306, 401, 644
BssMI GATC 2 cut(s) 302, 593
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 74, 263
BstAPI GCANNNNNTGC 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 460, 579
BstDEI CTNAG 3 cut(s) 467, 609, 711
BstDSI CCRYGG 1 cut(s) 306
BstFNI CGCG 2 cut(s) 107, 184
BstKTI GATC 2 cut(s) 305, 596
BstMAI GTCTC 2 cut(s) 349, 363
BstMBI GATC 2 cut(s) 302, 593
BstMWI GCNNNNNNNGC 4 cut(s) 17, 440, 468, 675
BstSCI CCNGG 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 259
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 2 cut(s) 107, 184
BstV1I GCAGC 5 cut(s) 7, 221, 442, 449, 593
BstX2I RGATCY 2 cut(s) 302, 593
BstYI RGATCY 2 cut(s) 302, 593
BsuRI GGCC 2 cut(s) 414, 548
BtgI CCRYGG 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 460, 579
CciI TCATGA 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 658
Cfr13I GGNCC 2 cut(s) 152, 686
CseI GACGC 1 cut(s) 113
Csp6I GTAC 2 cut(s) 588, 641
CspAI ACCGGT 1 cut(s) 658
CviAII CATG 7 cut(s) 44, 173, 190, 307, 544, 574, 702
CviQI GTAC 2 cut(s) 588, 641
DdeI CTNAG 3 cut(s) 467, 609, 711
DpnI GATC 2 cut(s) 304, 595
DpnII GATC 2 cut(s) 302, 593
DraIII CACNNNGTG 1 cut(s) 17
DrdI GACNNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 2 cut(s) 152, 686
Eco57I CTGAAG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 686
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 7 cut(s) 47, 176, 193, 310, 547, 577, 705
FatI CATG 7 cut(s) 43, 172, 189, 306, 543, 573, 701
FauI CCCGC 1 cut(s) 467
FblI GTMKAC 2 cut(s) 179, 258
Fnu4HI GCNGC 5 cut(s) 21, 210, 456, 463, 582
Fsp4HI GCNGC 5 cut(s) 21, 210, 456, 463, 582
GluI GCNGC 5 cut(s) 21, 210, 456, 463, 582
HaeIII GGCC 2 cut(s) 414, 548
HapII CCGG 2 cut(s) 403, 659
HgaI GACGC 1 cut(s) 113
Hin1II CATG 7 cut(s) 47, 176, 193, 310, 547, 577, 705
HinfI GANTC 2 cut(s) 169, 186
HpaII CCGG 2 cut(s) 403, 659
HphI GGTGA 2 cut(s) 173, 711
Hpy166II GTNNAC 3 cut(s) 180, 259, 434
Hpy188I TCNGA 2 cut(s) 130, 714
Hpy188III TCNNGA 3 cut(s) 44, 155, 486
Hpy8I GTNNAC 3 cut(s) 180, 259, 434
HpyAV CCTTC 2 cut(s) 152, 404
HpyCH4III ACNGT 2 cut(s) 74, 263
HpyCH4V TGCA 5 cut(s) 11, 23, 209, 434, 577
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 440, 468, 675
HpyF3I CTNAG 3 cut(s) 467, 609, 711
Hsp92II CATG 7 cut(s) 47, 176, 193, 310, 547, 577, 705
Kzo9I GATC 2 cut(s) 302, 593
LmnI GCTCC 1 cut(s) 666
Lsp1109I GCAGC 5 cut(s) 7, 221, 442, 449, 593
MaeIII GTNAC 2 cut(s) 74, 473
MalI GATC 2 cut(s) 304, 595
MboI GATC 2 cut(s) 302, 593
MboII GAAGA 5 cut(s) 32, 59, 309, 312, 546
MflI RGATCY 2 cut(s) 302, 593
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 2 cut(s) 6, 393
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 2 cut(s) 163, 195
MmeI TCCRAC 2 cut(s) 472, 621
MnlI CCTC 7 cut(s) 369, 372, 476, 545, 639, 699, 720
Mox20I TGGCCA 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 51
MscI TGGCCA 1 cut(s) 548
MseI TTAA 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 2 cut(s) 403, 659
MspR9I CCNGG 1 cut(s) 403
Mva1269I GAATGC 1 cut(s) 610
MvnI CGCG 2 cut(s) 107, 184
MwoI GCNNNNNNNGC 4 cut(s) 17, 440, 468, 675
NciI CCSGG 1 cut(s) 403
NcoI CCATGG 1 cut(s) 306
NdeII GATC 2 cut(s) 302, 593
NlaIII CATG 7 cut(s) 47, 176, 193, 310, 547, 577, 705
NlaIV GGNNCC 2 cut(s) 196, 687
NmuCI GTSAC 2 cut(s) 74, 473
PagI TCATGA 1 cut(s) 43
PctI GAATGC 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 51
PinAI ACCGGT 1 cut(s) 658
PkrI GCNGC 5 cut(s) 22, 211, 457, 464, 583
PleI GAGTC 2 cut(s) 163, 194
PpsI GAGTC 2 cut(s) 163, 194
PpuMI RGGWCCY 1 cut(s) 686
Psp5II RGGWCCY 1 cut(s) 686
PspN4I GGNNCC 2 cut(s) 196, 687
PspPI GGNCC 2 cut(s) 152, 686
PspPPI RGGWCCY 1 cut(s) 686
PsuI RGATCY 2 cut(s) 302, 593
RsaI GTAC 2 cut(s) 589, 642
RsaNI GTAC 2 cut(s) 588, 641
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 5 cut(s) 21, 210, 456, 463, 582
Sau3AI GATC 2 cut(s) 302, 593
Sau96I GGNCC 2 cut(s) 152, 686
SchI GAGTC 2 cut(s) 163, 195
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 2 cut(s) 152, 686
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 2 cut(s) 6, 393
SsiI CCGC 2 cut(s) 107, 460
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 74, 263
TaqI TCGA 3 cut(s) 282, 408, 734
TasI AATT 2 cut(s) 6, 393
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TseFI GTSAC 2 cut(s) 74, 473
TseI GCWGC 5 cut(s) 20, 209, 455, 462, 581
Tsp45I GTSAC 2 cut(s) 74, 473
TspDTI ATGAA 3 cut(s) 17, 32, 60
VneI GTGCAC 1 cut(s) 432
VpaK11BI GGWCC 2 cut(s) 152, 686
XmiI GTMKAC 2 cut(s) 179, 258
XmnI GAANNNNTTC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.