Rroxscaffold_5G00381430

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000641
Physical Location & Seq
Reverse (-)
61412256 .. 61416281
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00381430.1

Sequence Viewer

Length: 1338 bp
ATGGGTTTGGGAGCTGAGAATGGGAAAGTGAAAGTGGAGTCTTGGGATATAAGCAAATCCAAGGGGAGGAGGAAGAAGAAGATGGTAGTGGATGATGATGATGATGATGTGGAGGAAGAGGAGACCGGGTGCTGGCTAAGGCTGAGGTTTTTTGGGAGCTGCATTTCTTCAAGATCCAAAGTTGATAGCTCAATTAGTGGCACCAGTACTCACTGTGAAATCAAATCTACAAATGATACAAGCAGAGACCAACCGACGGCTACTGCAGTGTCTAACTCAACCACTAGTAACACAGAAAGTAATGCATCCACTTCAAAATTTGAAGAGGAACTTAAAGTTGCTTCAAGGTTGCGAAAATTTGCTTTTAACGATCTTAAATTGGCGACAAGAAATTTCAGACCTGAAAGTCTTCTTGGTGAAGGTGGTTTTGGTTGTGTGTTCAAGGGGTGGATAGAAGAAAATGGAACTGCTCCAGTCAAACCTGGCACAGGACTTACTGTTGCGGTTAAAACTCTCAATCATGATGGGCTTCAGGGTCATAAAGAATGGCTGGTTTGGACATGGTCCCTGCCTCTTCCATGGTCTATTAGAATGAAAATTGCATTGGGTGCGGCAAAAGGTCTTGCTTTTCTTCATGAGGAGGCAGAAAGACCTGTGATTTATCGTGACTTTAAAACCTCCAACATCTTACTGGATGCAGATTACAATGCCAAACTTTCTGATTTTGGACTTGCCAAAGATGGCCCAGAGGGTGATAAAACTCATGTCTCCACACGTGTGATGGGAACTTATGGTTATGCAGCTCCAGAATATGTAATGACTGGACATCTTACATCGAGGAGTGATGTCTATAGTTTCGGCGTAGTTTTACTTGAGATGCTGACAGGCAGAAGATCTATGGACAAAAATCGACCTAATGGTGAACATAACCTTGTGGAATGGGCTCGACCACATCTAGGAGATAGAAGAAGATTCTACCGATTAATAGACCCTCGGCTGGAAGGTCACTTTTCAATTAAAGGTGTACAGAAAGCTGCACAACTAGCTGCCCATTGCCTTAGTCGAGATCCAAAAGCAAGGCCCCTAATGAGCGATGTTGTGGAAGCATTAAAGCCATTGCCAAACCTTAAGGATATGGCAAGCTCATCATATTATTTCCAGACCATGCAAGGTGACCGAGTTGGATCCAGTCCGAATACCAGAAATGGTGTCAGAACACAAGGAGGATTGCTTACAAGGAATGGGCACAGGAGCCTATCAATACCAAATGGTTCCCATGCTTCTCCATTTCACCATCAATTTCCCCATCCATCTCCGAAACCTAATGGAAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

445

Amino Acids

49.31

Weight (kDa)

9.23

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 195 - 370 1.2e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 199 - 368 1.7e-27 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 2 cut(s) 503, 611
AclWI GGATC 4 cut(s) 168, 1061, 1179, 1192
AcsI RAATTY 3 cut(s) 317, 356, 391
AcuI CTGAAG 1 cut(s) 515
AcvI CACGTG 1 cut(s) 776
AfaI GTAC 2 cut(s) 208, 1026
AfiI CCNNNNNNNGG 6 cut(s) 66, 132, 256, 488, 956, 997
AflII CTTAAG 1 cut(s) 1127
AflIII ACRYGT 2 cut(s) 773, 775
AgsI TTSAA 6 cut(s) 171, 315, 323, 345, 442, 1014
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 6 cut(s) 396, 411, 428, 443, 587, 619
AleI CACNNNNGTG 1 cut(s) 776
AluBI AGCT 7 cut(s) 14, 159, 189, 803, 1034, 1046, 1143
AluI AGCT 7 cut(s) 14, 159, 189, 803, 1034, 1046, 1143
Alw26I GTCTC 3 cut(s) 116, 240, 772
AlwI GGATC 4 cut(s) 168, 1061, 1179, 1192
AoxI GGCC 2 cut(s) 742, 1079
ApeKI GCWGC 4 cut(s) 159, 800, 1034, 1046
ApoI RAATTY 3 cut(s) 317, 356, 391
AseI ATTAAT 1 cut(s) 983
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 564, 743, 1080
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 5 cut(s) 428, 764, 932, 1184, 1283
AvaII GGWCC 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 1248
BamHI GGATCC 1 cut(s) 1184
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 946
BbrPI CACGTG 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 401
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 4 cut(s) 146, 812, 1021, 1033
BccI CCATC 7 cut(s) 76, 518, 734, 775, 1302, 1314, 1318
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 483
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 3 cut(s) 116, 240, 772
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 3 cut(s) 285, 956, 1043
BfmI CTRYAG 2 cut(s) 264, 850
BfrI CTTAAG 1 cut(s) 1127
BglII AGATCT 1 cut(s) 893
BisI GCNGC 5 cut(s) 160, 612, 801, 1035, 1047
BlsI GCNGC 5 cut(s) 161, 613, 802, 1036, 1048
BmcAI AGTACT 1 cut(s) 208
Bme1390I CCNGG 2 cut(s) 127, 483
Bme18I GGWCC 1 cut(s) 564
BmgT120I GGNCC 3 cut(s) 564, 743, 1080
BmiI GGNNCC 6 cut(s) 202, 566, 1082, 1186, 1253, 1273
BmrFI CCNGG 2 cut(s) 127, 483
BmsI GCATC 3 cut(s) 314, 685, 867
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 2 cut(s) 456, 789
Bpu10I CCTNAGC 2 cut(s) 137, 143
BpuEI CTTGAG 1 cut(s) 893
BpuMI CCSGG 1 cut(s) 127
BsaAI YACGTR 1 cut(s) 776
BsaI GGTCTC 2 cut(s) 116, 240
BsaJI CCNNGG 3 cut(s) 60, 578, 992
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 132, 256, 488, 956, 997
Bse1I ACTGG 5 cut(s) 204, 473, 696, 826, 1188
Bse3DI GCAATG 2 cut(s) 1051, 1115
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 3 cut(s) 60, 578, 992
BseGI GGATG 4 cut(s) 97, 305, 700, 1306
BseLI CCNNNNNNNGG 6 cut(s) 66, 132, 256, 488, 956, 997
BseMI GCAATG 2 cut(s) 1051, 1115
BseMII CTCAG 2 cut(s) 6, 134
BseNI ACTGG 5 cut(s) 204, 473, 696, 826, 1188
BseRI GAGGAG 4 cut(s) 82, 134, 653, 853
BseSI GKGCMC 1 cut(s) 1248
BseXI GCAGC 4 cut(s) 146, 812, 1021, 1033
BsgI GTGCAG 1 cut(s) 1020
BshFI GGCC 2 cut(s) 744, 1081
BshNI GGYRCC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 6 cut(s) 66, 132, 256, 488, 956, 997
BsmAI GTCTC 3 cut(s) 116, 240, 772
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 2 cut(s) 744, 1081
Bso31I GGTCTC 2 cut(s) 116, 240
Bsp1286I GDGCHC 2 cut(s) 946, 1248
Bsp1407I TGTACA 1 cut(s) 1024
Bsp143I GATC 5 cut(s) 173, 370, 893, 1066, 1184
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 2 cut(s) 503, 611
BspANI GGCC 2 cut(s) 744, 1081
BspCNI CTCAG 2 cut(s) 7, 135
BspHI TCATGA 2 cut(s) 520, 634
BspLI GGNNCC 6 cut(s) 202, 566, 1082, 1186, 1253, 1273
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 4 cut(s) 168, 1061, 1179, 1192
BspT107I GGYRCC 1 cut(s) 200
BspTI CTTAAG 1 cut(s) 1127
BspTNI GGTCTC 2 cut(s) 116, 240
BsrDI GCAATG 2 cut(s) 1051, 1115
BsrGI TGTACA 1 cut(s) 1024
BsrI ACTGG 5 cut(s) 204, 473, 696, 826, 1188
BssECI CCNNGG 3 cut(s) 60, 578, 992
BssMI GATC 5 cut(s) 173, 370, 893, 1066, 1184
BssT1I CCWWGG 2 cut(s) 60, 578
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 215, 499
Bst6I CTCTTC 3 cut(s) 111, 318, 579
BstAFI CTTAAG 1 cut(s) 1127
BstAPI GCANNNNNTGC 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 1024
BstBAI YACGTR 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 134, 1141
BstDEI CTNAG 4 cut(s) 15, 137, 143, 1058
BstDSI CCRYGG 1 cut(s) 578
BstEII GGTNACC 1 cut(s) 1172
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 4 cut(s) 97, 305, 700, 1306
BstKTI GATC 5 cut(s) 176, 373, 896, 1069, 1187
BstMAI GTCTC 3 cut(s) 116, 240, 772
BstMBI GATC 5 cut(s) 173, 370, 893, 1066, 1184
BstMWI GCNNNNNNNGC 2 cut(s) 608, 1043
BstNI CCWGG 1 cut(s) 483
BstPI GGTNACC 1 cut(s) 1172
BstSCI CCNGG 2 cut(s) 125, 481
BstSFI CTRYAG 2 cut(s) 264, 850
BstSLI GKGCMC 1 cut(s) 1248
BstV1I GCAGC 4 cut(s) 146, 812, 1021, 1033
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 4 cut(s) 173, 893, 1066, 1184
BstYI RGATCY 4 cut(s) 173, 893, 1066, 1184
BsuRI GGCC 2 cut(s) 744, 1081
BtgI CCRYGG 1 cut(s) 578
BtgZI GCGATG 1 cut(s) 1107
BtsCI GGATG 4 cut(s) 97, 305, 700, 1306
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 211, 273
Cac8I GCNNGC 2 cut(s) 134, 1141
CciI TCATGA 2 cut(s) 520, 634
Cfr13I GGNCC 3 cut(s) 564, 743, 1080
Csp6I GTAC 2 cut(s) 207, 1025
CviAII CATG 7 cut(s) 521, 561, 579, 635, 764, 1165, 1277
CviQI GTAC 2 cut(s) 207, 1025
DdeI CTNAG 4 cut(s) 15, 137, 143, 1058
DpnI GATC 5 cut(s) 175, 372, 895, 1068, 1186
DpnII GATC 5 cut(s) 173, 370, 893, 1066, 1184
DraI TTTAAA 1 cut(s) 673
DrdI GACNNNNNNGTC 1 cut(s) 405
DseDI GACNNNNNNGTC 1 cut(s) 405
Eam1104I CTCTTC 3 cut(s) 111, 318, 579
EarI CTCTTC 3 cut(s) 111, 318, 579
Eco130I CCWWGG 2 cut(s) 60, 578
Eco24I GRGCYC 1 cut(s) 946
Eco31I GGTCTC 2 cut(s) 116, 240
Eco47I GGWCC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 515
Eco72I CACGTG 1 cut(s) 776
Eco91I GGTNACC 1 cut(s) 1172
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 1080
EcoO65I GGTNACC 1 cut(s) 1172
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 60, 578
EcoT22I ATGCAT 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 946
ErhI CCWWGG 2 cut(s) 60, 578
FaeI CATG 7 cut(s) 524, 564, 582, 638, 767, 1168, 1280
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 550
FatI CATG 7 cut(s) 520, 560, 578, 634, 763, 1164, 1276
Fnu4HI GCNGC 5 cut(s) 160, 612, 801, 1035, 1047
FokI GGATG 4 cut(s) 104, 292, 707, 1293
FriOI GRGCYC 1 cut(s) 946
Fsp4HI GCNGC 5 cut(s) 160, 612, 801, 1035, 1047
FspBI CTAG 3 cut(s) 285, 956, 1043
GluI GCNGC 5 cut(s) 160, 612, 801, 1035, 1047
GsuI CTGGAG 2 cut(s) 456, 789
HaeIII GGCC 2 cut(s) 744, 1081
HapII CCGG 1 cut(s) 126
Hin1II CATG 7 cut(s) 524, 564, 582, 638, 767, 1168, 1280
HinfI GANTC 2 cut(s) 38, 972
HpaII CCGG 1 cut(s) 126
HphI GGTGA 5 cut(s) 428, 764, 932, 1184, 1283
Hpy166II GTNNAC 2 cut(s) 923, 1025
Hpy188I TCNGA 5 cut(s) 398, 721, 1194, 1214, 1317
Hpy188III TCNNGA 7 cut(s) 171, 521, 635, 665, 806, 1064, 1159
Hpy8I GTNNAC 2 cut(s) 923, 1025
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 413, 995
HpyCH4III ACNGT 2 cut(s) 215, 499
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 8 cut(s) 162, 266, 305, 602, 698, 800, 1037, 1168
HpyF10VI GCNNNNNNNGC 2 cut(s) 608, 1043
HpyF3I CTNAG 4 cut(s) 15, 137, 143, 1058
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 7 cut(s) 524, 564, 582, 638, 767, 1168, 1280
Kzo9I GATC 5 cut(s) 173, 370, 893, 1066, 1184
LmnI GCTCC 5 cut(s) 11, 156, 475, 808, 1251
Lsp1109I GCAGC 4 cut(s) 146, 812, 1021, 1033
LweI GCATC 3 cut(s) 314, 685, 867
MaeI CTAG 3 cut(s) 285, 956, 1043
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 4 cut(s) 287, 665, 1004, 1172
MalI GATC 5 cut(s) 175, 372, 895, 1068, 1186
MboI GATC 5 cut(s) 173, 370, 893, 1066, 1184
MflI RGATCY 4 cut(s) 173, 893, 1066, 1184
MhlI GDGCHC 2 cut(s) 946, 1248
MluCI AATT 8 cut(s) 192, 317, 356, 377, 391, 597, 1014, 1298
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 2 cut(s) 705, 1162
Mph1103I ATGCAT 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 408
MslI CAYNNNNRTG 1 cut(s) 776
MspCI CTTAAG 1 cut(s) 1127
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 2 cut(s) 127, 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 2 cut(s) 608, 1043
NciI CCSGG 1 cut(s) 127
NcoI CCATGG 1 cut(s) 578
NdeII GATC 5 cut(s) 173, 370, 893, 1066, 1184
NlaIII CATG 7 cut(s) 524, 564, 582, 638, 767, 1168, 1280
NlaIV GGNNCC 6 cut(s) 202, 566, 1082, 1186, 1253, 1273
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 3 cut(s) 665, 1004, 1172
NsiI ATGCAT 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 776
PagI TCATGA 2 cut(s) 520, 634
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 972
PflFI GACNNNGTC 1 cut(s) 562
PkrI GCNGC 5 cut(s) 161, 613, 802, 1036, 1048
PleI GAGTC 1 cut(s) 46
PmaCI CACGTG 1 cut(s) 776
PmlI CACGTG 1 cut(s) 776
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 776
PshBI ATTAAT 1 cut(s) 983
Psp6I CCWGG 1 cut(s) 481
PspCI CACGTG 1 cut(s) 776
PspEI GGTNACC 1 cut(s) 1172
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 6 cut(s) 202, 566, 1082, 1186, 1253, 1273
PspPI GGNCC 3 cut(s) 564, 743, 1080
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 4 cut(s) 173, 893, 1066, 1184
PsyI GACNNNGTC 1 cut(s) 562
RsaI GTAC 2 cut(s) 208, 1026
RsaNI GTAC 2 cut(s) 207, 1025
RseI CAYNNNNRTG 1 cut(s) 776
SatI GCNGC 5 cut(s) 160, 612, 801, 1035, 1047
Sau3AI GATC 5 cut(s) 173, 370, 893, 1066, 1184
Sau96I GGNCC 3 cut(s) 564, 743, 1080
ScaI AGTACT 1 cut(s) 208
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 127, 483
SduI GDGCHC 2 cut(s) 946, 1248
SfaNI GCATC 3 cut(s) 314, 685, 867
SfcI CTRYAG 2 cut(s) 264, 850
SinI GGWCC 1 cut(s) 564
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 2 cut(s) 872, 1127
SmoI CTYRAG 2 cut(s) 872, 1127
SpeI ACTAGT 1 cut(s) 284
Sse9I AATT 8 cut(s) 192, 317, 356, 377, 391, 597, 1014, 1298
SsiI CCGC 2 cut(s) 503, 611
SspMI CTAG 3 cut(s) 285, 956, 1043
StyD4I CCNGG 2 cut(s) 125, 481
StyI CCWWGG 2 cut(s) 60, 578
TaaI ACNGT 2 cut(s) 215, 499
TaiI ACGT 1 cut(s) 778
TaqI TCGA 4 cut(s) 836, 910, 946, 1063
TaqII GACCGA 1 cut(s) 1191
TasI AATT 8 cut(s) 192, 317, 356, 377, 391, 597, 1014, 1298
TatI WGTACW 2 cut(s) 206, 1024
TauI GCSGC 1 cut(s) 614
TfiI GAWTC 1 cut(s) 972
TscAI CASTG 2 cut(s) 218, 273
TseFI GTSAC 3 cut(s) 665, 1004, 1172
TseI GCWGC 4 cut(s) 159, 800, 1034, 1046
Tsp45I GTSAC 3 cut(s) 665, 1004, 1172
TspDTI ATGAA 2 cut(s) 608, 623
TspRI CASTG 2 cut(s) 218, 273
Tth111I GACNNNGTC 1 cut(s) 562
Vha464I CTTAAG 1 cut(s) 1127
VpaK11BI GGWCC 1 cut(s) 564
VspI ATTAAT 1 cut(s) 983
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 3 cut(s) 317, 356, 391
XcmI CCANNNNNNNNNTGG 2 cut(s) 688, 778
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 3 cut(s) 285, 956, 1043
ZrmI AGTACT 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.