RchiOBHm_Chr2g0104451

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000641
Physical Location & Seq
Reverse (-)
15800777 .. 15801931
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47872

Sequence Viewer

Length: 591 bp
ATGATGGGCTTCAAGGTCATAAAGAATGGCTTGTTTGGACGTGCTGAAGTAAATTTCCTTGGTGACCTTGTCCCTCCAAACTTGGTAAAACTTATTGGATACTGCATTGAAGATGATCAAAGGCTGCTAGTGTATGAGTTCATGCCTCGGGGGAGCTTGGAGAACCACCTATTTAGGAGGTCCCTGCCTCTTCCATGGTCTATTAGAATGAAAATTGCATTGGGTGCAGCAAAAGGTCTTGCTTTTCTTCATGAGGAGGCAGAAAGACCTGTGATTTATCGTGACTTTAAAACCTCCAACATCTTATTGGATGCAGATTACAATGCCAAACTTTCTGATTTTGGACTTGCCAAAGATGGCCCGGAGGGTGATAAAACTCATGTCTCCACACGTGTGATGGGAACTTATGGTTATGCAGCTCCAGAATATGTAATGACTGGACATCTTACATCGTGGAGTGATGTCTATAGTTTTGGCGTAGTTTTCAAGGCTATGGGAAGAGCTATCAACAAGACTGTGTCGATTGTGGAGCTAATTAAGGTTAGATGTCTCATTGTGTATCACTATGCTGGATGGTTATATGTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

196

Amino Acids

22.16

Weight (kDa)

8.77

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 15 - 162 8.9e-31 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 15 - 165 3.5e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 66
AcvI CACGTG 1 cut(s) 392
AflIII ACRYGT 2 cut(s) 389, 391
AgsI TTSAA 3 cut(s) 13, 110, 487
AjiI CACGTC 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AleI CACNNNNGTG 1 cut(s) 392
AluBI AGCT 4 cut(s) 156, 419, 503, 532
AluI AGCT 4 cut(s) 156, 419, 503, 532
Alw26I GTCTC 2 cut(s) 388, 554
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 1 cut(s) 358
ApeKI GCWGC 3 cut(s) 124, 227, 416
ApoI RAATTY 1 cut(s) 52
AspS9I GGNCC 2 cut(s) 180, 359
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 2 cut(s) 74, 380
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 180
BbrPI CACGTG 1 cut(s) 392
BbvI GCAGC 3 cut(s) 111, 239, 428
BccI CCATC 3 cut(s) 350, 391, 567
BciVI GTATCC 1 cut(s) 92
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 388, 554
BfaI CTAG 2 cut(s) 128, 589
BfmI CTRYAG 1 cut(s) 466
BfuI GTATCC 1 cut(s) 92
BisI GCNGC 3 cut(s) 125, 228, 417
BlsI GCNGC 3 cut(s) 126, 229, 418
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 1 cut(s) 147
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 2 cut(s) 180, 359
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 301
BpmI CTGGAG 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 392
BsaJI CCNNGG 3 cut(s) 58, 146, 194
Bse1I ACTGG 1 cut(s) 442
BseDI CCNNGG 3 cut(s) 58, 146, 194
BseGI GGATG 2 cut(s) 316, 578
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 3 cut(s) 111, 239, 428
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 2 cut(s) 56, 166
BsmAI GTCTC 2 cut(s) 388, 554
BsmFI GGGAC 2 cut(s) 56, 166
BsnI GGCC 1 cut(s) 360
BsoBI CYCGRG 1 cut(s) 147
Bsp143I GATC 1 cut(s) 115
Bsp19I CCATGG 1 cut(s) 194
BspANI GGCC 1 cut(s) 360
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 182
BspQI GCTCTTC 1 cut(s) 493
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 3 cut(s) 58, 146, 194
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 58, 194
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 2 cut(s) 195, 493
BstAPI GCANNNNNTGC 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 392
BstDSI CCRYGG 1 cut(s) 194
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 316, 578
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 2 cut(s) 388, 554
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 1 cut(s) 466
BstV1I GCAGC 3 cut(s) 111, 239, 428
BsuI GTATCC 1 cut(s) 92
BsuRI GGCC 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 41
BtsCI GGATG 2 cut(s) 316, 578
CciI TCATGA 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 180, 359
CviAII CATG 4 cut(s) 142, 195, 251, 380
CviJI RGCY 9 cut(s) 9, 30, 124, 156, 360, 419, 491, 503, 532
CviKI_1 RGCY 9 cut(s) 9, 30, 124, 156, 360, 419, 491, 503, 532
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
DraI TTTAAA 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 195, 493
EarI CTCTTC 2 cut(s) 195, 493
Eco130I CCWWGG 2 cut(s) 58, 194
Eco47I GGWCC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 66
Eco72I CACGTG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 147
Eco91I GGTNACC 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 180
EcoO65I GGTNACC 1 cut(s) 62
EcoT14I CCWWGG 2 cut(s) 58, 194
ErhI CCWWGG 2 cut(s) 58, 194
FaeI CATG 4 cut(s) 145, 198, 254, 383
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 2 cut(s) 56, 166
FatI CATG 4 cut(s) 141, 194, 250, 379
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 125, 228, 417
FokI GGATG 2 cut(s) 323, 585
Fsp4HI GCNGC 3 cut(s) 125, 228, 417
FspBI CTAG 2 cut(s) 128, 589
GluI GCNGC 3 cut(s) 125, 228, 417
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 1 cut(s) 360
HapII CCGG 1 cut(s) 362
Hin1II CATG 4 cut(s) 145, 198, 254, 383
HpaII CCGG 1 cut(s) 362
HphI GGTGA 2 cut(s) 74, 380
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 3 cut(s) 251, 281, 422
Hpy8I GTNNAC 1 cut(s) 586
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4IV ACGT 2 cut(s) 40, 391
HpyCH4V TGCA 5 cut(s) 105, 218, 227, 314, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpySE526I ACGT 2 cut(s) 40, 391
Hsp92II CATG 4 cut(s) 145, 198, 254, 383
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 1 cut(s) 115
LguI GCTCTTC 1 cut(s) 493
LmnI GCTCC 3 cut(s) 153, 424, 529
LpnPI CCDG 6 cut(s) 197, 282, 375, 423, 435, 555
Lsp1109I GCAGC 3 cut(s) 111, 239, 428
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 128, 589
MaeII ACGT 2 cut(s) 40, 391
MaeIII GTNAC 2 cut(s) 62, 281
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 4 cut(s) 122, 182, 239, 510
MluCI AATT 3 cut(s) 52, 213, 534
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 8 cut(s) 84, 156, 171, 198, 247, 250, 304, 358
MseI TTAA 2 cut(s) 288, 537
MslI CAYNNNNRTG 1 cut(s) 392
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 1 cut(s) 362
NcoI CCATGG 1 cut(s) 194
NdeII GATC 1 cut(s) 115
NlaIII CATG 4 cut(s) 145, 198, 254, 383
NlaIV GGNNCC 1 cut(s) 182
NmuCI GTSAC 2 cut(s) 62, 281
OliI CACNNNNGTG 1 cut(s) 392
PagI TCATGA 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 493
PflFI GACNNNGTC 2 cut(s) 68, 517
PkrI GCNGC 3 cut(s) 126, 229, 418
PmaCI CACGTG 1 cut(s) 392
PmlI CACGTG 1 cut(s) 392
Ppu21I YACGTR 1 cut(s) 392
PpuMI RGGWCCY 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 180
PspCI CACGTG 1 cut(s) 392
PspEI GGTNACC 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 2 cut(s) 180, 359
PspPPI RGGWCCY 1 cut(s) 180
PsyI GACNNNGTC 2 cut(s) 68, 517
RseI CAYNNNNRTG 1 cut(s) 392
SapI GCTCTTC 1 cut(s) 493
SaqAI TTAA 2 cut(s) 288, 537
SatI GCNGC 3 cut(s) 125, 228, 417
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 2 cut(s) 180, 359
ScrFI CCNGG 1 cut(s) 362
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 466
SinI GGWCC 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 392
Sse9I AATT 3 cut(s) 52, 213, 534
SspMI CTAG 2 cut(s) 128, 589
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 2 cut(s) 58, 194
TaaI ACNGT 1 cut(s) 517
TaiI ACGT 2 cut(s) 43, 394
TaqI TCGA 1 cut(s) 521
TasI AATT 3 cut(s) 52, 213, 534
Tru1I TTAA 2 cut(s) 288, 537
Tru9I TTAA 2 cut(s) 288, 537
TseFI GTSAC 2 cut(s) 62, 281
TseI GCWGC 3 cut(s) 124, 227, 416
Tsp45I GTSAC 2 cut(s) 62, 281
TspDTI ATGAA 3 cut(s) 130, 224, 239
Tth111I GACNNNGTC 2 cut(s) 68, 517
VpaK11BI GGWCC 1 cut(s) 180
XapI RAATTY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 394
XspI CTAG 2 cut(s) 128, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.