FvH4_7g01242

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001468
Physical Location & Seq
Forward (+)
1649213 .. 1653521
4309 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01242.t1

Sequence Viewer

Length: 477 bp
ATGAAGAAGATAGTGAAGAAAGCATTGGGAAAGATTAATGTGATGAAAAGGAGGAACATGAGCAGAAGAGATGGGTGTGGTAGAGGAGAGGTTGAGGCAAAATGTGTACCATCATCAAGTGAGGCAGGCACCACGGTCGACGAAAAGGTTTTGGAGCTCCAAGAGAAGCTTTCTCAATGCCGACTCGAAAGGGACCGGCTCCAGGCCGAGAAAGAGAGTTTCATGATCATGAAGCGGGAATTAAAAAAACTAGAAGATGAACTCAGAGATGAGAAAGAAGCGAATTCGCTGGCGACTTCTATATTCAATGAAGAAAAGAAGTCACTCGAAGAGGTCTTGAAGGCCAAGTTGCGGAGTCTTCGTCAGTTCGAGAACGAATTTGATGAGTTTTATGGTAAACATCCTTTAGGGGAAAGAGTGGACAACCAGTTTGAGCATCCTGGAGAAGCGTCAATAGGAGTGGACAACGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

18.23

Weight (kDa)

6.24

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 138
AciI CCGC 2 cut(s) 235, 352
AcsI RAATTY 2 cut(s) 283, 377
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 202, 351
AgsI TTSAA 2 cut(s) 307, 340
AjnI CCWGG 2 cut(s) 201, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 2 cut(s) 157, 169
AluI AGCT 2 cut(s) 157, 169
Alw21I GWGCWC 1 cut(s) 159
AoxI GGCC 2 cut(s) 204, 342
ApoI RAATTY 2 cut(s) 283, 377
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
AseI ATTAAT 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 159
BccI CCATC 2 cut(s) 65, 118
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 203, 441
BclI TGATCA 1 cut(s) 225
BfaI CTAG 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 203, 441
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 3 cut(s) 130, 194, 200
BmrFI CCNGG 2 cut(s) 203, 441
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 350
BpmI CTGGAG 2 cut(s) 185, 462
BsaJI CCNNGG 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 351
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 427
BseBI CCWGG 2 cut(s) 203, 441
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 400, 436
BseLI CCNNNNNNNGG 2 cut(s) 202, 351
BseMII CTCAG 1 cut(s) 277
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 206, 344
BshNI GGYRCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 1 cut(s) 196
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 202, 351
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 2 cut(s) 206, 344
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 1 cut(s) 225
BspACI CCGC 2 cut(s) 235, 352
BspANI GGCC 2 cut(s) 206, 344
BspCNI CTCAG 1 cut(s) 276
BspHI TCATGA 2 cut(s) 222, 228
BspLI GGNNCC 3 cut(s) 130, 194, 200
BspT107I GGYRCC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 203, 441
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 2 cut(s) 61, 324
BstC8I GCNNGC 2 cut(s) 127, 291
BstDEI CTNAG 2 cut(s) 263, 474
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 400, 436
BstKTI GATC 1 cut(s) 228
BstMBI GATC 1 cut(s) 225
BstMCI CGRYCG 1 cut(s) 138
BstNI CCWGG 2 cut(s) 203, 441
BstSCI CCNGG 2 cut(s) 201, 439
BstV2I GAAGAC 1 cut(s) 350
BsuRI GGCC 2 cut(s) 206, 344
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 400, 436
Cac8I GCNNGC 2 cut(s) 127, 291
CciI TCATGA 2 cut(s) 222, 228
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 438
Csp6I GTAC 1 cut(s) 107
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 3 cut(s) 58, 223, 229
CviJI RGCY 5 cut(s) 157, 169, 199, 206, 344
CviKI_1 RGCY 5 cut(s) 157, 169, 199, 206, 344
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 2 cut(s) 263, 474
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 61, 324
EarI CTCTTC 2 cut(s) 61, 324
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 201, 439
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 3 cut(s) 61, 226, 232
FaiI YATR 5 cut(s) 59, 224, 230, 302, 393
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FaqI GGGAC 1 cut(s) 206
FatI CATG 3 cut(s) 57, 222, 228
FauI CCCGC 1 cut(s) 228
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 138
FokI GGATG 2 cut(s) 387, 423
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 1 cut(s) 251
GsuI CTGGAG 2 cut(s) 185, 462
HaeIII GGCC 2 cut(s) 206, 344
HapII CCGG 1 cut(s) 196
HgaI GACGC 1 cut(s) 438
Hin1II CATG 3 cut(s) 61, 226, 232
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 3 cut(s) 183, 355, 470
HpaII CCGG 1 cut(s) 196
Hpy166II GTNNAC 5 cut(s) 107, 139, 398, 421, 463
Hpy188I TCNGA 1 cut(s) 266
Hpy188III TCNNGA 4 cut(s) 223, 229, 337, 370
Hpy8I GTNNAC 5 cut(s) 107, 139, 398, 421, 463
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 1 cut(s) 136
HpyF3I CTNAG 2 cut(s) 263, 474
Hsp92II CATG 3 cut(s) 61, 226, 232
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 3 cut(s) 154, 162, 204
LpnPI CCDG 8 cut(s) 111, 188, 209, 215, 275, 426, 440, 453
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 251
MaeIII GTNAC 1 cut(s) 321
MalI GATC 1 cut(s) 227
MboI GATC 1 cut(s) 225
MboII GAAGA 8 cut(s) 16, 19, 28, 78, 266, 323, 341, 350
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 3 cut(s) 239, 283, 377
MlyI GAGTC 2 cut(s) 177, 364
MnlI CCTC 6 cut(s) 45, 77, 82, 88, 115, 325
MseI TTAA 2 cut(s) 36, 242
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 2 cut(s) 203, 441
MvaI CCWGG 2 cut(s) 203, 441
NdeII GATC 1 cut(s) 225
NlaIII CATG 3 cut(s) 61, 226, 232
NlaIV GGNNCC 3 cut(s) 130, 194, 200
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 321
PagI TCATGA 2 cut(s) 222, 228
PfeI GAWTC 1 cut(s) 470
PfoI TCCNGGA 1 cut(s) 439
PleI GAGTC 2 cut(s) 177, 363
PpsI GAGTC 2 cut(s) 177, 363
PshBI ATTAAT 1 cut(s) 36
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 201, 439
PspGI CCWGG 2 cut(s) 201, 439
PspN4I GGNNCC 3 cut(s) 130, 194, 200
PspPI GGNCC 1 cut(s) 193
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 159
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 2 cut(s) 36, 242
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 2 cut(s) 177, 364
ScrFI CCNGG 2 cut(s) 203, 441
SduI GDGCHC 1 cut(s) 159
SetI ASST 5 cut(s) 93, 150, 159, 171, 336
SfaNI GCATC 1 cut(s) 445
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 3 cut(s) 239, 283, 377
SsiI CCGC 2 cut(s) 235, 352
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 201, 439
TaaI ACNGT 1 cut(s) 136
TaqI TCGA 4 cut(s) 138, 186, 327, 369
TasI AATT 3 cut(s) 239, 283, 377
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 36, 242
Tru9I TTAA 2 cut(s) 36, 242
TseFI GTSAC 1 cut(s) 321
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 6 cut(s) 17, 59, 211, 245, 273, 324
VpaK11BI GGWCC 1 cut(s) 193
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 2 cut(s) 283, 377
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.