RLG00000030669

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001468
Physical Location & Seq
Reverse (-)
65430714 .. 65431695
982 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030669

Sequence Viewer

Length: 312 bp
ATGGTGATGGTGGTGGTGGCATTCATGAAATTAACTGCTCTTTTCACGAGCAAAATCTATACGCAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAATAGCCTTTGGAAACGCAAAGCTGATGTTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTCGTAGCAATTCCAGGCTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTGCAAACATACATACTTTGTTGCCTCCACCATACGAATCAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

104

Amino Acids

11.47

Weight (kDa)

6.03

Isoelectric Point (pI)

62.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 227
AclWI GGATC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 227
AjnI CCWGG 2 cut(s) 94, 187
AluBI AGCT 2 cut(s) 122, 298
AluI AGCT 2 cut(s) 122, 298
AlwI GGATC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 64
AspLEI GCGC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 16
BauI CACGAG 1 cut(s) 46
BbvI GCAGC 1 cut(s) 76
BccI CCATC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 96, 189
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 1 cut(s) 65
BlpI GCTNAGC 2 cut(s) 74, 90
BlsI GCNGC 1 cut(s) 66
Bme1390I CCNGG 2 cut(s) 96, 189
BmrFI CCNGG 2 cut(s) 96, 189
BmsI GCATC 1 cut(s) 193
Bpu1102I GCTNAGC 2 cut(s) 74, 90
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 139
BseBI CCWGG 2 cut(s) 96, 189
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 227
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 227
BsmI GAATGC 1 cut(s) 20
Bsp143I GATC 1 cut(s) 238
Bsp1720I GCTNAGC 2 cut(s) 74, 90
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 24
BspPI GGATC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 1 cut(s) 238
BssSI CACGAG 1 cut(s) 46
Bst2BI CACGAG 1 cut(s) 46
Bst2UI CCWGG 2 cut(s) 96, 189
BstAPI GCANNNNNTGC 1 cut(s) 70
BstDEI CTNAG 3 cut(s) 74, 90, 309
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 1 cut(s) 241
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 70, 128
BstNI CCWGG 2 cut(s) 96, 189
BstSCI CCNGG 2 cut(s) 94, 187
BstV1I GCAGC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 208
CciI TCATGA 1 cut(s) 24
CfoI GCGC 1 cut(s) 220
CviAII CATG 2 cut(s) 25, 136
CviJI RGCY 7 cut(s) 67, 78, 94, 105, 122, 192, 298
CviKI_1 RGCY 7 cut(s) 67, 78, 94, 105, 122, 192, 298
DdeI CTNAG 3 cut(s) 74, 90, 309
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 94, 187
EcoT22I ATGCAT 1 cut(s) 141
FaeI CATG 2 cut(s) 28, 139
FaiI YATR 9 cut(s) 26, 60, 87, 137, 152, 254, 258, 277, 306
FatI CATG 2 cut(s) 24, 135
Fnu4HI GCNGC 1 cut(s) 65
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 65
GlaI GCGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 221
HhaI GCGC 1 cut(s) 220
Hin1II CATG 2 cut(s) 28, 139
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HinfI GANTC 1 cut(s) 281
HphI GGTGA 1 cut(s) 16
Hpy188III TCNNGA 2 cut(s) 25, 46
HpyCH4V TGCA 4 cut(s) 139, 144, 206, 248
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 128
HpyF3I CTNAG 3 cut(s) 74, 90, 309
Hsp92II CATG 2 cut(s) 28, 139
HspAI GCGC 1 cut(s) 218
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 6 cut(s) 81, 108, 174, 201, 213, 222
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 1 cut(s) 193
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MluCI AATT 2 cut(s) 29, 183
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 2 cut(s) 160, 279
Mph1103I ATGCAT 1 cut(s) 141
MseI TTAA 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 96, 189
Mva1269I GAATGC 1 cut(s) 20
MvaI CCWGG 2 cut(s) 96, 189
MwoI GCNNNNNNNGC 2 cut(s) 70, 128
NdeII GATC 1 cut(s) 238
NlaIII CATG 2 cut(s) 28, 139
NsiI ATGCAT 1 cut(s) 141
PagI TCATGA 1 cut(s) 24
PctI GAATGC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 281
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 94, 187
PspGI CCWGG 2 cut(s) 94, 187
PsrI GAACNNNNNNTAC 1 cut(s) 282
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 96, 189
SetI ASST 3 cut(s) 124, 211, 300
SfaNI GCATC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 149
Sse9I AATT 2 cut(s) 29, 183
StyD4I CCNGG 2 cut(s) 94, 187
TasI AATT 2 cut(s) 29, 183
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseI GCWGC 1 cut(s) 64
TspDTI ATGAA 4 cut(s) 13, 41, 124, 167
Van91I CCANNNNNTGG 1 cut(s) 227
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.