Rh1DG017500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001468
Physical Location & Seq
Forward (+)
2855671 .. 2856661
991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG017500.1

Sequence Viewer

Length: 660 bp
ATGTTGATGGCGGATGAGGCAGGCACCATGGACGAAGAGGTTTTCAAGCTTAAAGAGAAGCTTTCTCAATGCCAACTCGAAAGGGAGCAGCTCCTGAGAGAGAAAGAGAGCTGGATGATCAAGGAGCAACTTAAGAAGCCACTCAAGGAGGCCTTGGAGACCAAAAAGAAGAGTTTACGTCAGCTAAAAAACGCTTTCGATGAGTTTCATGCTAATCGTGAAATGCCTTATATGGGAGCACGATCAAACAATGCCAATGACAAGTTAAAGAGATTGCGTGCTCAATTAGAAAAGGAAAATAAGTGCTCACAAGTAGAGATAGATAAGCTGGACAAGCTACATTCAGAGATAATTGAGAAACGGGACAATTTATTTGCTTTTCTTAAGACTTGTGTGAAAAATTCAAAACTCACTGATGATGTAAAACCCTTGCTCCGAGTCCACTATGATGCTTATATTGACAAACTCAGGACTGAAGATGATGATTGTGAACTGTTAAGAAAGTATGTGCATTTTGTTCTACAACCGGGTTCTGCTCACAAGCAAAATGAGATGTTTGCCGGGGTTGATTTTTTATCTCCAATTTCTGCTGCAATGTCAAAATTTTTTAACATCTTCCTGAGAAAAAAAGACAGAGTCCAATGGTTCTCTTATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

25.95

Weight (kDa)

8.86

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AciI CCGC 1 cut(s) 11
AcsI RAATTY 2 cut(s) 400, 602
AcuI CTGAAG 1 cut(s) 495
AfiI CCNNNNNNNGG 1 cut(s) 233
AflII CTTAAG 2 cut(s) 131, 383
AgsI TTSAA 2 cut(s) 46, 405
AluBI AGCT 7 cut(s) 49, 61, 91, 111, 184, 328, 337
AluI AGCT 7 cut(s) 49, 61, 91, 111, 184, 328, 337
Alw21I GWGCWC 3 cut(s) 241, 283, 308
Alw26I GTCTC 1 cut(s) 152
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 88, 590
ApoI RAATTY 2 cut(s) 400, 602
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AsuC2I CCSGG 2 cut(s) 528, 562
BanI GGYRCC 1 cut(s) 23
Bbv12I GWGCWC 3 cut(s) 241, 283, 308
BbvI GCAGC 2 cut(s) 100, 577
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 528, 562
BcoDI GTCTC 1 cut(s) 152
BfrI CTTAAG 2 cut(s) 131, 383
BisI GCNGC 2 cut(s) 89, 591
BlsI GCNGC 2 cut(s) 90, 592
Bme1390I CCNGG 2 cut(s) 528, 562
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 2 cut(s) 528, 562
BmsI GCATC 1 cut(s) 439
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 2 cut(s) 528, 562
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 27, 153, 561
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 27, 153, 561
BseGI GGATG 2 cut(s) 19, 120
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 3 cut(s) 86, 481, 611
BseXI GCAGC 2 cut(s) 100, 577
BshFI GGCC 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 23
BsiHKAI GWGCWC 3 cut(s) 241, 283, 308
BsiSI CCGG 2 cut(s) 527, 561
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 152
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 3 cut(s) 241, 283, 308
Bsp143I GATC 2 cut(s) 117, 242
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 1 cut(s) 11
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 3 cut(s) 87, 480, 612
BspLI GGNNCC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 23
BspTI CTTAAG 2 cut(s) 131, 383
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 600
BssECI CCNNGG 3 cut(s) 27, 153, 561
BssMI GATC 2 cut(s) 117, 242
BssT1I CCWWGG 2 cut(s) 27, 153
Bst4CI ACNGT 1 cut(s) 495
Bst6I CTCTTC 2 cut(s) 30, 164
BstAFI CTTAAG 2 cut(s) 131, 383
BstC8I GCNNGC 2 cut(s) 22, 279
BstDEI CTNAG 3 cut(s) 95, 467, 620
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 19, 120
BstKTI GATC 2 cut(s) 120, 245
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 2 cut(s) 117, 242
BstMWI GCNNNNNNNGC 2 cut(s) 17, 334
BstSCI CCNGG 2 cut(s) 526, 560
BstV1I GCAGC 2 cut(s) 100, 577
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 19, 120
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 22, 279
CaiI CAGNNNCTG 1 cut(s) 94
CviAII CATG 2 cut(s) 28, 209
CviJI RGCY 9 cut(s) 49, 61, 91, 111, 139, 152, 184, 328, 337
CviKI_1 RGCY 9 cut(s) 49, 61, 91, 111, 139, 152, 184, 328, 337
DdeI CTNAG 3 cut(s) 95, 467, 620
DpnI GATC 2 cut(s) 119, 244
DpnII GATC 2 cut(s) 117, 242
Eam1104I CTCTTC 2 cut(s) 30, 164
EarI CTCTTC 2 cut(s) 30, 164
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 27, 153
Eco147I AGGCCT 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 495
EcoT14I CCWWGG 2 cut(s) 27, 153
ErhI CCWWGG 2 cut(s) 27, 153
FaeI CATG 2 cut(s) 31, 212
FaiI YATR 8 cut(s) 29, 210, 231, 233, 447, 456, 507, 658
FalI AAGNNNNNCTT 4 cut(s) 45, 77, 137, 169
FaqI GGGAC 1 cut(s) 377
FatI CATG 2 cut(s) 27, 208
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 89, 591
FokI GGATG 2 cut(s) 26, 127
Fsp4HI GCNGC 2 cut(s) 89, 591
GluI GCNGC 2 cut(s) 89, 591
HaeIII GGCC 1 cut(s) 152
HapII CCGG 2 cut(s) 527, 561
Hin1II CATG 2 cut(s) 31, 212
HindIII AAGCTT 2 cut(s) 47, 59
HinfI GANTC 2 cut(s) 438, 636
HpaII CCGG 2 cut(s) 527, 561
Hpy166II GTNNAC 3 cut(s) 176, 442, 491
Hpy188I TCNGA 2 cut(s) 346, 437
Hpy188III TCNNGA 4 cut(s) 94, 218, 469, 619
Hpy8I GTNNAC 3 cut(s) 176, 442, 491
HpyCH4III ACNGT 1 cut(s) 495
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 2 cut(s) 511, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 334
HpyF3I CTNAG 3 cut(s) 95, 467, 620
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 2 cut(s) 31, 212
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 117, 242
LmnI GCTCC 5 cut(s) 85, 96, 124, 236, 438
LpnPI CCDG 8 cut(s) 6, 97, 107, 314, 454, 540, 574, 632
Lsp1109I GCAGC 2 cut(s) 100, 577
LweI GCATC 1 cut(s) 439
MaeII ACGT 1 cut(s) 178
MalI GATC 2 cut(s) 119, 244
MboI GATC 2 cut(s) 117, 242
MboII GAAGA 4 cut(s) 47, 181, 488, 607
MhlI GDGCHC 3 cut(s) 241, 283, 308
MluCI AATT 6 cut(s) 284, 351, 367, 400, 582, 602
MlyI GAGTC 2 cut(s) 447, 645
MnlI CCTC 3 cut(s) 10, 31, 142
MseI TTAA 6 cut(s) 51, 132, 266, 384, 497, 609
MslI CAYNNNNRTG 1 cut(s) 447
MspCI CTTAAG 2 cut(s) 131, 383
MspI CCGG 2 cut(s) 527, 561
MspR9I CCNGG 2 cut(s) 528, 562
MwoI GCNNNNNNNGC 2 cut(s) 17, 334
NciI CCSGG 2 cut(s) 528, 562
NcoI CCATGG 1 cut(s) 27
NdeII GATC 2 cut(s) 117, 242
NlaIII CATG 2 cut(s) 31, 212
NlaIV GGNNCC 1 cut(s) 25
PceI AGGCCT 1 cut(s) 152
PflFI GACNNNGTC 1 cut(s) 635
PkrI GCNGC 2 cut(s) 90, 592
PleI GAGTC 2 cut(s) 446, 644
PpsI GAGTC 2 cut(s) 446, 644
PspN4I GGNNCC 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 94
PsyI GACNNNGTC 1 cut(s) 635
RseI CAYNNNNRTG 1 cut(s) 447
SaqAI TTAA 6 cut(s) 51, 132, 266, 384, 497, 609
SatI GCNGC 2 cut(s) 89, 591
Sau3AI GATC 2 cut(s) 117, 242
SchI GAGTC 2 cut(s) 447, 645
ScrFI CCNGG 2 cut(s) 528, 562
SduI GDGCHC 3 cut(s) 241, 283, 308
SetI ASST 9 cut(s) 42, 51, 63, 93, 113, 181, 186, 330, 339
SfaNI GCATC 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 3 cut(s) 131, 143, 383
SmoI CTYRAG 3 cut(s) 131, 143, 383
Sse9I AATT 6 cut(s) 284, 351, 367, 400, 582, 602
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 526, 560
StyI CCWWGG 2 cut(s) 27, 153
TaaI ACNGT 1 cut(s) 495
TaiI ACGT 1 cut(s) 181
TaqI TCGA 2 cut(s) 78, 198
TasI AATT 6 cut(s) 284, 351, 367, 400, 582, 602
Tru1I TTAA 6 cut(s) 51, 132, 266, 384, 497, 609
Tru9I TTAA 6 cut(s) 51, 132, 266, 384, 497, 609
TscAI CASTG 1 cut(s) 418
TseI GCWGC 2 cut(s) 88, 590
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 1 cut(s) 418
Tth111I GACNNNGTC 1 cut(s) 635
Vha464I CTTAAG 2 cut(s) 131, 383
XapI RAATTY 2 cut(s) 400, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.