Rorug01G0012900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001468
Physical Location & Seq
Forward (+)
2121401 .. 2128908
7508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0012900.1

Sequence Viewer

Length: 3759 bp
ATGATGAAGAAACGCCGCGTTCCGCAGGTCCTCTGGCGCCTCTTCCACCACCGCGCCCGCACTCTGGCCCACACTCTCACCTCCTTACTCCCTCCTCATTCCTCCTCTCCAAACGCCCAATGTCGTTTCTGCAAGGGCCGCCGTTGCCTCAGCTGCTGCACCGGCCCCGACGCCGTTTCGTTTCTCCTCCGACCCGACGATCCCTCCGATTACCGCTCTCTCCTCGACAAGTGCTTCGTCGTCGTCTCCGAAAACGCGCCGGCCATCACTCGCTTCTCCCCTGAATCTCACTGGTCCCAAATTGAGATCGTTAGAGCAGTCGTCGAAACGACGATGCTGGAGCAATCTATGTCTTCCAATGTGATATGCAATGGTTATGATAAGCGTGACAAGTCAAGTGCCATTGTGGAACTTCTGACTTCTTCGGCATGGTGTCGTCTCTTAGAAAGGGTTGGTGATGGCGTTATGGTTTATTTACTAAAGAATGCGTCGATATTTTTGCGAATTCCGGATAAGAAACACCAGCAGGTGACAGGTCCTCCCATTAGTGACTTATGTCGTAAGAGGTTCTATTGCACACCGAATTCACAACGGCAACCGTCATCCCTTGCTCCATGTGGACCGCGGAAAAAGAGGAAAAGAGATGATAATGTTCACCCAATGTTAAAGAAAAAGCAGTTCATGAGTTCAGTCAGCATTGGAGAAAGCTCGAGTTCTGTTACCTGCAATGGCTGCTGTAACCATAGTGAAAATTATAGGCTTACGCATAGTCATATAAGTTTCTCTGAAGCATCTACATCAGCAACCAGCACGCTTACCAATGATTATGAAGGGAATTTAAATCAGGAACTTACAGAGAGTTTGGAAAATCTAAGAAAGCGTTCAAGACCATTTCGTTGGCAGCGCTGCAGGAAACGCAGACAGTTATCTTTGCAAGAAACTGGTGTTAAGGGTCTTTTCAGAACAACTGTCGGTGATAAACAATGGTTACCTGGCAGGCTTTCGTCTTCCTTGAAACCGAGCTTAGGTCGACATTATGAACAGTGCAACTGTTTGGGGTTCCAAGTTGCCCAAAAGGTTTCCAATGGTGCTCAAATAGACAGGAAATCCTTGTTCTTCAATTTGGAATCTTCTTCATCAGTCTTTCCTAAAAAGCATGTGCTGAATTCTGTAAAGCCTGATTCTGTTGGTTCAGAGTTTCTTGTTCAGAGTATTTTTGGCATATATGACATAGAAAGTGCACTGTCAAAGACATGTCCCAATGGCAGTGGTCTTTGTCTCATGGGATCTGCATGCCTATATCACTCGCTTGTTAAGTTGCTTAAAATTCTTATACGTAGAGCACAGTATGGCCATCATGTGAGATTACTGGAAAAGCATTGTGTTGTTAGAACTGTAAATCCTAATACAATCAAAAGCGGTGGTTGCATCTCTGAGGGAGAAAAGTGGGAGTACAATGTACTCAAGAAATCTCAGTGTTGTACTGCGGACCATTGCAATAAATCTCCAGAAGTTAGTGAATCTTTCTCTGAGGAAATTAAGTCTTACTGCTTGAACAGTCAGGTGGAGTCATTTATATGGGCTGTTTGTAGGAGTATTGTTCCTCCTGAGTTGCTAGGAACTCCTTCTAACTGGAGAATTTTGAGGAGAACTATTTCAAAGTTCATTCGACTTAGAAGATTTGAAAGGTTTTCCTTAAAACAATGTATGCATAAATTGAAAACGTCTAGGTTCCCGTTTCTATCAAATAAACATTATTTCTGCTGCATGAATAATGAGGCACCGAAACATGCGGATGGAAAAGGTGTAGACATTCAAAAGGGATCCAGTACATTGAATGATGCTGTACATATTGTTAAAGTAAAACTCTTAGAAAGTTGGATTTATTGGTTCTTTTCATCTGTAGTTGTGCCATTGTTGCAAGCCAATTTCTATGTCACTGAAAGTGAGCATGGGAAGCAAGATGTATATTACTACCGGAAGTCAGTTTGGGAGAAGGTGAAGAATAAGACCATCGTCACTTGCATGGATCAGAGCTATCAATATTTGGATGATGCGACAACTAGAAGAATTATAAGGAAACGATCATTTGGGTTTTCAAAGCTCCGGATTTGTCCAAAAGAGTATGGAGTGAGGTTGCTAGCAAATTTAAAAGCACCATCAAAATTGCCAGGGAAAGAGTCCTGTTTGGCAGATCAGTCTAGTGGAATATTTAGAGGAAAGAAACTGCATAGGAGAAAGGTTAGATTTGAGCATTTCAAGTCTGTGAATCGTGTTCTTCGTGATACACATGTTGTTCTAAAAGCTATGCAGCTGAAAGAGACAGGGAAATTAGGATCATCAGTGTTTGATTACAATGATGTATACAGAAAGTTGTGCCCATTTCTAATTGGCATGAAGAGTGGATTCACTATGATACCTGATGTGTTTATCATTGTCTCTGATGTATCAAAAGCCTTTGATTCTGTTGACCAGGATAAGCTGCTTGGTGTGCTGAAAGATGTTATAAGAGCAGATGAATATTTTCTCCAGCAGTCTCATCATGTCATTTTCAAGAAGGGTTCATTAAGGATTCATGAGAACCGTGCATTTCTAGATCAAAATACTGGCTCAAGATTTAAGTCTAAATTACGTAGTGTCCTTGTTAATCAGGAGTGCAGCAGGTCTGTGAAGAAGAAGGAGCTATTTTTTAATCTAAATGAGCATGTGAAGCGCAATGTGCTGCAGTTGGATAAAAAGTTTTATCTGCAACGTATCGGTATTCCTCAAGGAAGCGTTTTGTCAACTTTGCTTTGCTCATTATACTATGGACATCTTGATCAGAATGTGATCTTTCCATTTCTTGACAGAACTTGGGAACCTTCTATTCCAGATTTATCAAGAGGACATGATTTTCAAGTTGCTTCTGCTGCAGAGAGTAGCAGCGAGGATAAAATATCCTCATCTTCTAGATATACTCTACTTAGATTTATTGATGATTTTCTTTTCATTTCAACCTCAAAGGCGCAGGCTGCCAGCTTCTTCTCCAGGTTGCAAAGAGGATTTCGGGATTACAACTGCTACATGAACGAGAAAAAGTTCTGTGTAAATTTTGATATTGGAAACCTGCCATCAAACAGGGTCTACAGTGGTGATGATGGCATTTTATTTATTCGATGGAGTGGCTTGCTTATCAATTCTCGCACACTTGAAGTTCAGGCAGACTATACGAAGTACTTGAAAAACCATCTAAGTTCGACTGTTACTGTCCACTACCAAGGCAAACCTGGTCGCCATTTTAAGGGAAAGTTGCGTGACTACATGAGACCTAAGTGTCATCCCTTATTCTTTGATTCCCACATTAATTCAGCATCTGTTGTGAGATTAAATATCTACCAAGCATTCTTGCTATGTGCAATGAAGTTCCATTGTTATGTTCGTGACTTATCATATATCTGCAAATTTCACTTTGGATTTTACGCTTACAGTATCCAAAAATCTTTGAGGTACATGCACAGGCTCATAAAGAAAAGGATGTGTTCATTGCATACTGGCTCTAACTTCAGCCCAATTCTTCATTTGGAAAAGGGAGAAGTTGAATGGCTTGGTTTATATGCTTATATCCAAGTATTGAAGAGAAAACAATCACGGTATAAGGAGTTGCTCTCCTTATTGAAGTCCAAGTTGTTAAATCATAAGATAACCGGCTGCGTTTCTTCTGAGCTAAGTTATGCAGTTGATAGATCACATTCTTCTGTAATTTGGAAGATCAAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1252

Amino Acids

143.94

Weight (kDa)

9.68

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Telomerase_RBD PF12009 521 - 672 5.2e-36 Telomerase ribonucleoprotein complex - RNA binding domain
RVT_1 PF00078 886 - 1028 7.1e-06 Reverse transcriptase (RNA-dependent DNA polymerase)
TERT_C PF21399 1091 - 1218 7e-19 Telomerase reverse transcriptase, C-terminal extension
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2075, 2507
AarI CACCTGC 1 cut(s) 517
AasI GACNNNNNNGTC 1 cut(s) 3312
Acc36I ACCTGC 5 cut(s) 16, 517, 731, 2652, 3114
AccB1I GGYRCC 2 cut(s) 36, 1780
AccBSI CCGCTC 1 cut(s) 216
AccI GTMKAC 4 cut(s) 1030, 1809, 2364, 3123
AccII CGCG 4 cut(s) 18, 54, 257, 625
AccIII TCCGGA 2 cut(s) 508, 2106
AclWI GGATC 6 cut(s) 194, 1294, 1818, 1831, 2037, 2344
AcoI YGGCCR 2 cut(s) 261, 1351
AcsI RAATTY 9 cut(s) 504, 583, 835, 1165, 1326, 1638, 2146, 3088, 3440
AcuI CTGAAG 2 cut(s) 807, 3526
AcyI GRCGYC 2 cut(s) 37, 171
AfaI GTAC 7 cut(s) 1454, 1461, 1483, 1831, 1848, 3215, 3488
AfeI AGCGCT 1 cut(s) 905
AfiI CCNNNNNNNGG 3 cut(s) 64, 1025, 3280
AflIII ACRYGT 2 cut(s) 1253, 2290
AjnI CCWGG 5 cut(s) 991, 2170, 2472, 3026, 3265
AloI GAACNNNNNNTCC 2 cut(s) 1384, 1416
Alw21I GWGCWC 3 cut(s) 1093, 1243, 1345
Alw26I GTCTC 7 cut(s) 250, 443, 1283, 2315, 2443, 2541, 3298
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 6 cut(s) 194, 1294, 1818, 1831, 2037, 2344
AlwNI CAGNNNCTG 2 cut(s) 156, 3353
Ama87I CYCGRG 1 cut(s) 709
Aor13HI TCCGGA 2 cut(s) 508, 2106
Aor51HI AGCGCT 1 cut(s) 905
AoxI GGCC 5 cut(s) 66, 136, 163, 261, 1351
ApaLI GTGCAC 1 cut(s) 1239
ApoI RAATTY 9 cut(s) 504, 583, 835, 1165, 1326, 1638, 2146, 3088, 3440
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AseI ATTAAT 1 cut(s) 3342
Asp700I GAANNNNTTC 5 cut(s) 880, 1624, 1654, 2523, 3077
AspLEI GCGC 6 cut(s) 39, 56, 259, 906, 2715, 3007
AspS9I GGNCC 8 cut(s) 28, 67, 136, 164, 294, 536, 620, 1489
AsuHPI GGTGA 7 cut(s) 70, 467, 541, 647, 986, 2011, 3143
AsuNHI GCTAGC 1 cut(s) 2140
AvaI CYCGRG 1 cut(s) 709
AvaII GGWCC 5 cut(s) 28, 294, 536, 620, 1489
BaeGI GKGCMC 2 cut(s) 1243, 2381
BaeI ACNNNNGTAYC 2 cut(s) 2277, 2310
BalI TGGCCA 1 cut(s) 1353
BamHI GGATCC 1 cut(s) 1823
BanI GGYRCC 2 cut(s) 36, 1780
BbsI GAAGAC 2 cut(s) 345, 999
Bbv12I GWGCWC 3 cut(s) 1093, 1243, 1345
BbvCI CCTCAGC 1 cut(s) 149
BceAI ACGGC 3 cut(s) 126, 158, 608
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 5 cut(s) 993, 2172, 2474, 3028, 3267
BciVI GTATCC 1 cut(s) 3479
BclI TGATCA 1 cut(s) 2818
BcoDI GTCTC 7 cut(s) 250, 443, 1283, 2315, 2443, 2541, 3298
BfaI CTAG 7 cut(s) 1616, 1728, 2064, 2141, 2202, 2594, 2949
BfmI CTRYAG 5 cut(s) 907, 1902, 2723, 2910, 3124
BfoI RGCGCY 2 cut(s) 40, 907
BfuAI ACCTGC 5 cut(s) 16, 517, 731, 2652, 3114
BfuI GTATCC 1 cut(s) 3479
BmcAI AGTACT 1 cut(s) 3215
Bme1390I CCNGG 5 cut(s) 993, 2172, 2474, 3028, 3267
Bme18I GGWCC 5 cut(s) 28, 294, 536, 620, 1489
BmeT110I CYCGRG 1 cut(s) 709
BmgT120I GGNCC 8 cut(s) 28, 67, 136, 164, 294, 536, 620, 1489
BmiI GGNNCC 8 cut(s) 38, 166, 296, 1061, 1733, 1782, 1825, 2859
BmrFI CCNGG 5 cut(s) 993, 2172, 2474, 3028, 3267
BmsI GCATC 6 cut(s) 324, 800, 1437, 1831, 2044, 3359
BmtI GCTAGC 1 cut(s) 2144
BoxI GACNNNNGTC 2 cut(s) 555, 2015
BpiI GAAGAC 2 cut(s) 345, 999
BplI GAGNNNNNCTC 2 cut(s) 3629, 3661
BpmI CTGGAG 5 cut(s) 359, 1491, 1654, 2513, 3010
Bpu10I CCTNAGC 2 cut(s) 149, 1024
BpuEI CTTGAG 3 cut(s) 1448, 2596, 2751
BsaAI YACGTR 2 cut(s) 1337, 2633
BsaHI GRCGYC 2 cut(s) 37, 171
BsaI GGTCTC 1 cut(s) 3298
BsaJI CCNNGG 3 cut(s) 623, 2171, 3256
BsaWI WCCGGW 3 cut(s) 508, 1977, 2106
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 523, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 1025, 3280
Bse118I RCCGGY 3 cut(s) 161, 259, 3683
Bse1I ACTGG 7 cut(s) 296, 946, 1374, 1637, 1827, 2611, 3535
Bse3DI GCAATG 6 cut(s) 376, 733, 1492, 2722, 3402, 3521
BseAI TCCGGA 2 cut(s) 508, 2106
BseBI CCWGG 5 cut(s) 993, 2172, 2474, 3028, 3267
BseDI CCNNGG 3 cut(s) 623, 2171, 3256
BseGI GGATG 5 cut(s) 602, 1801, 2056, 3316, 3519
BseLI CCNNNNNNNGG 3 cut(s) 64, 1025, 3280
BseMI GCAATG 6 cut(s) 376, 733, 1492, 2722, 3402, 3521
BseMII CTCAG 6 cut(s) 163, 1425, 1487, 1521, 1599, 3690
BseNI ACTGG 7 cut(s) 296, 946, 1374, 1637, 1827, 2611, 3535
BseRI GAGGAG 5 cut(s) 84, 94, 176, 212, 1660
BseSI GKGCMC 2 cut(s) 1243, 2381
BsgI GTGCAG 2 cut(s) 142, 2677
Bsh1236I CGCG 4 cut(s) 18, 54, 257, 625
BshFI GGCC 5 cut(s) 68, 138, 165, 263, 1353
BshNI GGYRCC 2 cut(s) 36, 1780
BsiHKAI GWGCWC 3 cut(s) 1093, 1243, 1345
BsiHKCI CYCGRG 1 cut(s) 709
BsiSI CCGG 6 cut(s) 162, 260, 509, 1978, 2107, 3684
BslFI GGGAC 2 cut(s) 280, 1242
BslI CCNNNNNNNGG 3 cut(s) 64, 1025, 3280
BsmAI GTCTC 7 cut(s) 250, 443, 1283, 2315, 2443, 2541, 3298
BsmBI CGTCTC 2 cut(s) 250, 443
BsmFI GGGAC 2 cut(s) 280, 1242
BsmI GAATGC 2 cut(s) 490, 3380
BsnI GGCC 5 cut(s) 68, 138, 165, 263, 1353
Bso31I GGTCTC 1 cut(s) 3298
BsoBI CYCGRG 1 cut(s) 709
Bsp1286I GDGCHC 4 cut(s) 1093, 1243, 1345, 2381
Bsp13I TCCGGA 2 cut(s) 508, 2106
Bsp1407I TGTACA 1 cut(s) 1846
BspANI GGCC 5 cut(s) 68, 138, 165, 263, 1353
BspCNI CTCAG 6 cut(s) 162, 1426, 1486, 1522, 1600, 3691
BspEI TCCGGA 2 cut(s) 508, 2106
BspFNI CGCG 4 cut(s) 18, 54, 257, 625
BspHI TCATGA 2 cut(s) 681, 2575
BspLI GGNNCC 8 cut(s) 38, 166, 296, 1061, 1733, 1782, 1825, 2859
BspMAI CTGCAG 3 cut(s) 911, 2727, 2914
BspMI ACCTGC 5 cut(s) 16, 517, 731, 2652, 3114
BspOI GCTAGC 1 cut(s) 2144
BspPI GGATC 6 cut(s) 194, 1294, 1818, 1831, 2037, 2344
BspT107I GGYRCC 2 cut(s) 36, 1780
BspTNI GGTCTC 1 cut(s) 3298
BsrBI CCGCTC 1 cut(s) 216
BsrDI GCAATG 6 cut(s) 376, 733, 1492, 2722, 3402, 3521
BsrFI RCCGGY 3 cut(s) 161, 259, 3683
BsrGI TGTACA 1 cut(s) 1846
BsrI ACTGG 7 cut(s) 296, 946, 1374, 1637, 1827, 2611, 3535
BssAI RCCGGY 3 cut(s) 161, 259, 3683
BssECI CCNNGG 3 cut(s) 623, 2171, 3256
BssNAI GTATAC 1 cut(s) 2365
BssNI GRCGYC 2 cut(s) 37, 171
BssT1I CCWWGG 1 cut(s) 3256
Bst1107I GTATAC 1 cut(s) 2365
Bst2UI CCWGG 5 cut(s) 993, 2172, 2474, 3028, 3267
Bst6I CTCTTC 3 cut(s) 47, 2393, 3608
BstACI GRCGYC 2 cut(s) 37, 171
BstAPI GCANNNNNTGC 1 cut(s) 732
BstAUI TGTACA 1 cut(s) 1846
BstBAI YACGTR 2 cut(s) 1337, 2633
BstDSI CCRYGG 1 cut(s) 623
BstEII GGTNACC 1 cut(s) 987
BstF5I GGATG 5 cut(s) 602, 1801, 2056, 3316, 3519
BstFNI CGCG 4 cut(s) 18, 54, 257, 625
BstH2I RGCGCY 2 cut(s) 40, 907
BstHHI GCGC 6 cut(s) 39, 56, 259, 906, 2715, 3007
BstMAI GTCTC 7 cut(s) 250, 443, 1283, 2315, 2443, 2541, 3298
BstNI CCWGG 5 cut(s) 993, 2172, 2474, 3028, 3267
BstNSI RCATGY 7 cut(s) 1160, 1257, 1296, 1793, 2294, 2708, 3493
BstPAI GACNNNNGTC 2 cut(s) 555, 2015
BstPI GGTNACC 1 cut(s) 987
BstSCI CCNGG 5 cut(s) 991, 2170, 2472, 3026, 3265
BstSFI CTRYAG 5 cut(s) 907, 1902, 2723, 2910, 3124
BstSLI GKGCMC 2 cut(s) 1243, 2381
BstSNI TACGTA 2 cut(s) 1337, 2633
BstUI CGCG 4 cut(s) 18, 54, 257, 625
BstV2I GAAGAC 2 cut(s) 345, 999
BstX2I RGATCY 2 cut(s) 1286, 1823
BstXI CCANNNNNNTGG 1 cut(s) 897
BstYI RGATCY 2 cut(s) 1286, 1823
BstZ17I GTATAC 1 cut(s) 2365
BsuI GTATCC 1 cut(s) 3479
BsuRI GGCC 5 cut(s) 68, 138, 165, 263, 1353
BtgI CCRYGG 1 cut(s) 623
BtsCI GGATG 5 cut(s) 602, 1801, 2056, 3316, 3519
BtsI GCAGTG 1 cut(s) 1273
BtsIMutI CAGTG 8 cut(s) 289, 1049, 1241, 1273, 1481, 1938, 2349, 3133
BveI ACCTGC 5 cut(s) 16, 517, 731, 2652, 3114
CaiI CAGNNNCTG 2 cut(s) 156, 3353
CciI TCATGA 2 cut(s) 681, 2575
CfoI GCGC 6 cut(s) 39, 56, 259, 906, 2715, 3007
Cfr10I RCCGGY 3 cut(s) 161, 259, 3683
Cfr13I GGNCC 8 cut(s) 28, 67, 136, 164, 294, 536, 620, 1489
Cfr42I CCGCGG 1 cut(s) 626
CseI GACGC 2 cut(s) 179, 477
CsiI ACCWGGT 1 cut(s) 3265
Csp6I GTAC 7 cut(s) 1453, 1460, 1482, 1830, 1847, 3214, 3487
CspCI CAANNNNNGTGG 4 cut(s) 1249, 1284, 1402, 1437
CviQI GTAC 7 cut(s) 1453, 1460, 1482, 1830, 1847, 3214, 3487
DinI GGCGCC 1 cut(s) 38
DraI TTTAAA 2 cut(s) 840, 2151
DrdI GACNNNNNNGTC 1 cut(s) 3312
DseDI GACNNNNNNGTC 1 cut(s) 3312
EaeI YGGCCR 2 cut(s) 261, 1351
Eam1104I CTCTTC 3 cut(s) 47, 2393, 3608
EarI CTCTTC 3 cut(s) 47, 2393, 3608
Eco105I TACGTA 2 cut(s) 1337, 2633
Eco130I CCWWGG 1 cut(s) 3256
Eco31I GGTCTC 1 cut(s) 3298
Eco47I GGWCC 5 cut(s) 28, 294, 536, 620, 1489
Eco47III AGCGCT 1 cut(s) 905
Eco57I CTGAAG 2 cut(s) 807, 3526
Eco88I CYCGRG 1 cut(s) 709
Eco91I GGTNACC 1 cut(s) 987
EcoO109I RGGNCCY 2 cut(s) 28, 536
EcoO65I GGTNACC 1 cut(s) 987
EcoRI GAATTC 3 cut(s) 504, 583, 1165
EcoRII CCWGG 5 cut(s) 991, 2170, 2472, 3026, 3265
EcoT14I CCWWGG 1 cut(s) 3256
EcoT22I ATGCAT 1 cut(s) 1713
EgeI GGCGCC 1 cut(s) 38
EheI GGCGCC 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 3256
Esp3I CGTCTC 2 cut(s) 250, 443
FaqI GGGAC 2 cut(s) 280, 1242
FauI CCCGC 1 cut(s) 65
FbaI TGATCA 1 cut(s) 2818
FblI GTMKAC 4 cut(s) 1030, 1809, 2364, 3123
FokI GGATG 5 cut(s) 589, 1808, 2063, 3303, 3526
FspBI CTAG 7 cut(s) 1616, 1728, 2064, 2141, 2202, 2594, 2949
GlaI GCGC 6 cut(s) 38, 55, 258, 905, 2714, 3006
GsuI CTGGAG 5 cut(s) 359, 1491, 1654, 2513, 3010
HaeII RGCGCY 2 cut(s) 40, 907
HaeIII GGCC 5 cut(s) 68, 138, 165, 263, 1353
HapII CCGG 6 cut(s) 162, 260, 509, 1978, 2107, 3684
HgaI GACGC 2 cut(s) 179, 477
HhaI GCGC 6 cut(s) 39, 56, 259, 906, 2715, 3007
Hin1I GRCGYC 2 cut(s) 37, 171
Hin6I GCGC 6 cut(s) 37, 54, 257, 904, 2713, 3005
HinP1I GCGC 6 cut(s) 37, 54, 257, 904, 2713, 3005
HincII GTYRAC 3 cut(s) 1031, 2470, 2784
HindII GTYRAC 3 cut(s) 1031, 2470, 2784
HpaII CCGG 6 cut(s) 162, 260, 509, 1978, 2107, 3684
HphI GGTGA 7 cut(s) 70, 467, 541, 647, 986, 2011, 3143
Hpy99I CGWCG 7 cut(s) 173, 200, 242, 245, 326, 334, 493
HpyAV CCTTC 6 cut(s) 824, 1635, 1990, 2551, 2671, 2871
HpyCH4IV ACGT 4 cut(s) 1336, 1724, 2632, 2752
HpySE526I ACGT 4 cut(s) 1336, 1724, 2632, 2752
Hsp92I GRCGYC 2 cut(s) 37, 171
HspAI GCGC 6 cut(s) 37, 54, 257, 904, 2713, 3005
KasI GGCGCC 1 cut(s) 36
Kpn2I TCCGGA 2 cut(s) 508, 2106
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 2818
KspI CCGCGG 1 cut(s) 626
LmnI GCTCC 4 cut(s) 340, 616, 2109, 2680
LweI GCATC 6 cut(s) 324, 800, 1437, 1831, 2044, 3359
MabI ACCWGGT 1 cut(s) 3265
MaeI CTAG 7 cut(s) 1616, 1728, 2064, 2141, 2202, 2594, 2949
MaeII ACGT 4 cut(s) 1336, 1724, 2632, 2752
MbiI CCGCTC 1 cut(s) 216
MflI RGATCY 2 cut(s) 1286, 1823
MhlI GDGCHC 4 cut(s) 1093, 1243, 1345, 2381
MlsI TGGCCA 1 cut(s) 1353
MluNI TGGCCA 1 cut(s) 1353
Mly113I GGCGCC 1 cut(s) 37
MlyI GAGTC 2 cut(s) 1577, 2189
MmeI TCCRAC 3 cut(s) 214, 1859, 2709
Mox20I TGGCCA 1 cut(s) 1353
Mph1103I ATGCAT 1 cut(s) 1713
MroI TCCGGA 2 cut(s) 508, 2106
MroNI GCCGGC 1 cut(s) 259
MroXI GAANNNNTTC 5 cut(s) 880, 1624, 1654, 2523, 3077
MscI TGGCCA 1 cut(s) 1353
MslI CAYNNNNRTG 4 cut(s) 1576, 1794, 2024, 3411
Msp20I TGGCCA 1 cut(s) 1353
MspA1I CMGCKG 3 cut(s) 153, 625, 2314
MspI CCGG 6 cut(s) 162, 260, 509, 1978, 2107, 3684
MspR9I CCNGG 5 cut(s) 993, 2172, 2474, 3028, 3267
Mva1269I GAATGC 2 cut(s) 490, 3380
MvaI CCWGG 5 cut(s) 993, 2172, 2474, 3028, 3267
MvnI CGCG 4 cut(s) 18, 54, 257, 625
NaeI GCCGGC 1 cut(s) 261
NarI GGCGCC 1 cut(s) 37
NgoMIV GCCGGC 1 cut(s) 259
NheI GCTAGC 1 cut(s) 2140
NlaIV GGNNCC 8 cut(s) 38, 166, 296, 1061, 1733, 1782, 1825, 2859
NmuCI GTSAC 7 cut(s) 386, 529, 548, 1936, 2017, 3293, 3419
NsiI ATGCAT 1 cut(s) 1713
NspI RCATGY 7 cut(s) 1160, 1257, 1296, 1793, 2294, 2708, 3493
PaeI GCATGC 1 cut(s) 1296
PaeR7I CTCGAG 1 cut(s) 709
PagI TCATGA 2 cut(s) 681, 2575
PaqCI CACCTGC 1 cut(s) 517
PciI ACATGT 2 cut(s) 1253, 2290
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 246, 2278
PctI GAATGC 2 cut(s) 490, 3380
PdiI GCCGGC 1 cut(s) 261
PdmI GAANNNNTTC 5 cut(s) 880, 1624, 1654, 2523, 3077
PfeI GAWTC 9 cut(s) 284, 1127, 1181, 1520, 2269, 2406, 2462, 2572, 3332
PleI GAGTC 2 cut(s) 1576, 2188
PluTI GGCGCC 1 cut(s) 40
PpsI GAGTC 2 cut(s) 1576, 2188
Ppu21I YACGTR 2 cut(s) 1337, 2633
PpuMI RGGWCCY 2 cut(s) 28, 536
PscI ACATGT 2 cut(s) 1253, 2290
PshAI GACNNNNGTC 2 cut(s) 555, 2015
PshBI ATTAAT 1 cut(s) 3342
PsiI TTATAA 2 cut(s) 2075, 2507
Psp5II RGGWCCY 2 cut(s) 28, 536
Psp6I CCWGG 5 cut(s) 991, 2170, 2472, 3026, 3265
PspEI GGTNACC 1 cut(s) 987
PspGI CCWGG 5 cut(s) 991, 2170, 2472, 3026, 3265
PspN4I GGNNCC 8 cut(s) 38, 166, 296, 1061, 1733, 1782, 1825, 2859
PspPI GGNCC 8 cut(s) 28, 67, 136, 164, 294, 536, 620, 1489
PspPPI RGGWCCY 2 cut(s) 28, 536
PspXI VCTCGAGB 1 cut(s) 709
PstI CTGCAG 3 cut(s) 911, 2727, 2914
PstNI CAGNNNCTG 2 cut(s) 156, 3353
PsuI RGATCY 2 cut(s) 1286, 1823
PvuII CAGCTG 2 cut(s) 153, 2314
RsaI GTAC 7 cut(s) 1454, 1461, 1483, 1831, 1848, 3215, 3488
RsaNI GTAC 7 cut(s) 1453, 1460, 1482, 1830, 1847, 3214, 3487
RseI CAYNNNNRTG 4 cut(s) 1576, 1794, 2024, 3411
SacII CCGCGG 1 cut(s) 626
SalI GTCGAC 1 cut(s) 1029
Sau96I GGNCC 8 cut(s) 28, 67, 136, 164, 294, 536, 620, 1489
ScaI AGTACT 1 cut(s) 3215
SchI GAGTC 2 cut(s) 1577, 2189
ScrFI CCNGG 5 cut(s) 993, 2172, 2474, 3028, 3267
SduI GDGCHC 4 cut(s) 1093, 1243, 1345, 2381
SexAI ACCWGGT 1 cut(s) 3265
SfaNI GCATC 6 cut(s) 324, 800, 1437, 1831, 2044, 3359
SfcI CTRYAG 5 cut(s) 907, 1902, 2723, 2910, 3124
SfoI GGCGCC 1 cut(s) 38
Sfr274I CTCGAG 1 cut(s) 709
Sfr303I CCGCGG 1 cut(s) 626
SgrBI CCGCGG 1 cut(s) 626
SinI GGWCC 5 cut(s) 28, 294, 536, 620, 1489
SlaI CTCGAG 1 cut(s) 709
SmiI ATTTAAAT 1 cut(s) 840
SmiMI CAYNNNNRTG 4 cut(s) 1576, 1794, 2024, 3411
SmlI CTYRAG 4 cut(s) 709, 1463, 2611, 2766
SmoI CTYRAG 4 cut(s) 709, 1463, 2611, 2766
SnaBI TACGTA 2 cut(s) 1337, 2633
SphI GCATGC 1 cut(s) 1296
SspDI GGCGCC 1 cut(s) 36
SspI AATATT 3 cut(s) 2045, 2211, 2522
SspMI CTAG 7 cut(s) 1616, 1728, 2064, 2141, 2202, 2594, 2949
StyD4I CCNGG 5 cut(s) 991, 2170, 2472, 3026, 3265
StyI CCWWGG 1 cut(s) 3256
SwaI ATTTAAAT 1 cut(s) 840
TaiI ACGT 4 cut(s) 1339, 1727, 2635, 2755
TaqI TCGA 8 cut(s) 225, 324, 491, 710, 1030, 1669, 3154, 3236
TatI WGTACW 6 cut(s) 1452, 1459, 1481, 1829, 1846, 3213
TauI GCSGC 2 cut(s) 18, 141
TfiI GAWTC 9 cut(s) 284, 1127, 1181, 1520, 2269, 2406, 2462, 2572, 3332
TscAI CASTG 8 cut(s) 296, 1049, 1248, 1273, 1481, 1945, 2349, 3133
TseFI GTSAC 7 cut(s) 386, 529, 548, 1936, 2017, 3293, 3419
Tsp45I GTSAC 7 cut(s) 386, 529, 548, 1936, 2017, 3293, 3419
TspRI CASTG 8 cut(s) 296, 1049, 1248, 1273, 1481, 1945, 2349, 3133
VneI GTGCAC 1 cut(s) 1239
VpaK11BI GGWCC 5 cut(s) 28, 294, 536, 620, 1489
VspI ATTAAT 1 cut(s) 3342
XapI RAATTY 9 cut(s) 504, 583, 835, 1165, 1326, 1638, 2146, 3088, 3440
XbaI TCTAGA 2 cut(s) 2593, 2948
XceI RCATGY 7 cut(s) 1160, 1257, 1296, 1793, 2294, 2708, 3493
XcmI CCANNNNNNNNNTGG 1 cut(s) 3263
XhoI CTCGAG 1 cut(s) 709
XmiI GTMKAC 4 cut(s) 1030, 1809, 2364, 3123
XmnI GAANNNNTTC 5 cut(s) 880, 1624, 1654, 2523, 3077
XspI CTAG 7 cut(s) 1616, 1728, 2064, 2141, 2202, 2594, 2949
ZrmI AGTACT 1 cut(s) 3215
Zsp2I ATGCAT 1 cut(s) 1713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.