FvH4_7g30310

FK506-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000998
Physical Location & Seq
Forward (+)
22115669 .. 22117067
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30310.t1

Sequence Viewer

Length: 396 bp
ATGAAGAAAGTTGTGTTGAAGTTGGACGTACACGACGACAAGTCGAAGAAGAAAGCCATGAGGGCAGTCTCAGGGCTCGAAGGGCTAGATTCGATCTCCATGGACATGAAGGATCAGAAGCTGACAGTGACTGGGGACATAGATCCGGTAGGCTTGGTGAGCAAGTTGAGGAAGCTATGCCGCACTGAGATAGTGTCTGTAGGACCGGCGAAGGAGGAAAAGAAGAAGGAGGAGCCAAAGAAAGAGGAAGCCAAAAAGGGAGAGGATAAGAAGAAAGATCCAAAAGATGAAGTGGCTGAGCTTCTCAAGGCCTACAATGCATACAACCCTCCGATGAGTACTCATTATTATGTGAGAAGTGTGGAGGAGGATCCCAATGCCTGTGTCATCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

132

Amino Acids

14.71

Weight (kDa)

8.63

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 9 - 58 4.4e-10 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 181
AclWI GGATC 5 cut(s) 120, 137, 272, 365, 378
AfaI GTAC 2 cut(s) 30, 340
AgsI TTSAA 1 cut(s) 19
AhdI GACNNNNNGTC 1 cut(s) 40
AluBI AGCT 3 cut(s) 121, 175, 301
AluI AGCT 3 cut(s) 121, 175, 301
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 5 cut(s) 120, 137, 272, 365, 378
AlwNI CAGNNNCTG 2 cut(s) 121, 131
AoxI GGCC 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 169
AvaII GGWCC 1 cut(s) 203
BamHI GGATCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 86
BfmI CTRYAG 1 cut(s) 198
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 1 cut(s) 181
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 182
BmcAI AGTACT 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 203
BmeRI GACNNNNNGTC 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 234, 372
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 145
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 84, 177, 288
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 245, 380
BshFI GGCC 1 cut(s) 311
BsiSI CCGG 2 cut(s) 146, 206
BslFI GGGAC 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 5 cut(s) 93, 112, 142, 277, 370
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 1 cut(s) 181
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 3 cut(s) 83, 178, 289
BspLI GGNNCC 2 cut(s) 234, 372
BspPI GGATC 5 cut(s) 120, 137, 272, 365, 378
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 5 cut(s) 93, 112, 142, 277, 370
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 127
BstDEI CTNAG 3 cut(s) 70, 186, 297
BstDSI CCRYGG 1 cut(s) 99
BstKTI GATC 5 cut(s) 96, 115, 145, 280, 373
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 5 cut(s) 93, 112, 142, 277, 370
BstMWI GCNNNNNNNGC 4 cut(s) 62, 82, 159, 317
BstSFI CTRYAG 1 cut(s) 198
BstX2I RGATCY 3 cut(s) 142, 277, 370
BstYI RGATCY 3 cut(s) 142, 277, 370
BsuRI GGCC 1 cut(s) 311
BtgI CCRYGG 1 cut(s) 99
BtsIMutI CAGTG 2 cut(s) 132, 183
CaiI CAGNNNCTG 2 cut(s) 121, 131
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 29, 339
CviAII CATG 3 cut(s) 58, 100, 106
CviQI GTAC 2 cut(s) 29, 339
DdeI CTNAG 3 cut(s) 70, 186, 297
DpnI GATC 5 cut(s) 95, 114, 144, 279, 372
DpnII GATC 5 cut(s) 93, 112, 142, 277, 370
DriI GACNNNNNGTC 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 99
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 3 cut(s) 61, 103, 109
FaiI YATR 7 cut(s) 59, 101, 107, 140, 178, 322, 351
FaqI GGGAC 1 cut(s) 149
FatI CATG 3 cut(s) 57, 99, 105
Fnu4HI GCNGC 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 1 cut(s) 86
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 146, 206
Hin1II CATG 3 cut(s) 61, 103, 109
HinfI GANTC 1 cut(s) 89
HpaII CCGG 2 cut(s) 146, 206
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 117, 333
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 74, 103, 205, 220
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 82, 159, 317
HpyF3I CTNAG 3 cut(s) 70, 186, 297
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 3 cut(s) 61, 103, 109
Kzo9I GATC 5 cut(s) 93, 112, 142, 277, 370
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 4 cut(s) 57, 117, 159, 219
MaeI CTAG 1 cut(s) 86
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 127
MalI GATC 5 cut(s) 95, 114, 144, 279, 372
MboI GATC 5 cut(s) 93, 112, 142, 277, 370
MboII GAAGA 5 cut(s) 16, 58, 61, 235, 283
MflI RGATCY 3 cut(s) 142, 277, 370
MhlI GDGCHC 1 cut(s) 78
MnlI CCTC 9 cut(s) 54, 162, 208, 223, 238, 256, 339, 358, 361
Mph1103I ATGCAT 1 cut(s) 322
MslI CAYNNNNRTG 2 cut(s) 104, 348
MspI CCGG 2 cut(s) 146, 206
MwoI GCNNNNNNNGC 4 cut(s) 62, 82, 159, 317
NcoI CCATGG 1 cut(s) 99
NdeII GATC 5 cut(s) 93, 112, 142, 277, 370
NlaIII CATG 3 cut(s) 61, 103, 109
NlaIV GGNNCC 2 cut(s) 234, 372
NmuCI GTSAC 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 322
PceI AGGCCT 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 234, 372
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 2 cut(s) 121, 131
PsuI RGATCY 3 cut(s) 142, 277, 370
RsaI GTAC 2 cut(s) 30, 340
RsaNI GTAC 2 cut(s) 29, 339
RseI CAYNNNNRTG 2 cut(s) 104, 348
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 5 cut(s) 93, 112, 142, 277, 370
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 340
SduI GDGCHC 1 cut(s) 78
SetI ASST 4 cut(s) 30, 123, 177, 303
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 104, 348
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SseBI AGGCCT 1 cut(s) 311
SsiI CCGC 1 cut(s) 181
SspMI CTAG 1 cut(s) 86
StuI AGGCCT 1 cut(s) 311
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 44, 78, 92
TatI WGTACW 1 cut(s) 338
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 89
TscAI CASTG 2 cut(s) 132, 190
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 17, 122, 303
TspRI CASTG 2 cut(s) 132, 190
VpaK11BI GGWCC 1 cut(s) 203
XspI CTAG 1 cut(s) 86
ZrmI AGTACT 1 cut(s) 340
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.