MD14G1111400.v1.1

late blight resistance protein homolog R1B-19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000998
Physical Location & Seq
Forward (+)
17944891 .. 17946342
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1111400.v1.1.491

Sequence Viewer

Length: 402 bp
ATGAAGAAGGTGGTACTGAAATTGGATTTACATGATGACAAGGAAAAACAAAAGGCCTTGAAAGCAGTCTCCAGCCTTACAGGCATTGACTCAATTGCTATGGACATGAAGGAGAAGAAACTAACGGTGATCGGCGACGTTGACCCGGTCGATGTGGTGGCCAAACTGAGAAGAGGGTGGTACACAGAGATCCTAACAGTCGGGCCGGCAAAGGAAGAAAAGAAAGATGATAAGAAAGACGGAGGGGGTGCCAAAAAAGATGACAAGAAAGAAGATGATAAGAAGAAACCTGATCCAGTTGCTGAGCTTGTCAAGGCCTACCAAGCATACAATCCCCACATGACCAAGTACTACTATGTCCAAAGTGCTGAAGAAAACCCGAATTCCTGTGTTATCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

134

Amino Acids

14.94

Weight (kDa)

8.35

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 9 - 61 4.5e-10 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 248
AclWI GGATC 2 cut(s) 184, 287
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 3 cut(s) 15, 182, 350
AgsI TTSAA 1 cut(s) 61
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 2 cut(s) 184, 287
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 4 cut(s) 54, 159, 203, 315
ApoI RAATTY 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 139
BalI TGGCCA 1 cut(s) 161
BanI GGYRCC 1 cut(s) 248
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 73
BglI GCCNNNNNGGC 1 cut(s) 81
BlpI GCTNAGC 1 cut(s) 303
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 296
BseMII CTCAG 2 cut(s) 158, 294
BseNI ACTGG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 4 cut(s) 56, 161, 205, 317
BshNI GGYRCC 1 cut(s) 248
BsiEI CGRYCG 1 cut(s) 150
BsiSI CCGG 2 cut(s) 146, 206
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 4 cut(s) 56, 161, 205, 317
Bsp143I GATC 3 cut(s) 129, 189, 292
Bsp1720I GCTNAGC 1 cut(s) 303
BspANI GGCC 4 cut(s) 56, 161, 205, 317
BspCNI CTCAG 2 cut(s) 159, 295
BspLI GGNNCC 1 cut(s) 250
BspPI GGATC 2 cut(s) 184, 287
BspT107I GGYRCC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 205
BssMI GATC 3 cut(s) 129, 189, 292
Bst4CI ACNGT 2 cut(s) 127, 199
Bst6I CTCTTC 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 2 cut(s) 167, 303
BstKTI GATC 3 cut(s) 132, 192, 295
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 3 cut(s) 129, 189, 292
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 3 cut(s) 62, 81, 323
BstSCI CCNGG 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BsuRI GGCC 4 cut(s) 56, 161, 205, 317
Cac8I GCNNGC 1 cut(s) 207
CaiI CAGNNNCTG 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 3 cut(s) 14, 181, 349
CviAII CATG 3 cut(s) 32, 106, 340
CviJI RGCY 6 cut(s) 56, 75, 161, 205, 307, 317
CviKI_1 RGCY 6 cut(s) 56, 75, 161, 205, 307, 317
CviQI GTAC 3 cut(s) 14, 181, 349
DdeI CTNAG 2 cut(s) 167, 303
DpnI GATC 3 cut(s) 131, 191, 294
DpnII GATC 3 cut(s) 129, 189, 292
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
Eco147I AGGCCT 2 cut(s) 56, 317
Eco57I CTGAAG 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 382
FaeI CATG 3 cut(s) 35, 109, 343
FaiI YATR 6 cut(s) 33, 101, 107, 328, 341, 357
FatI CATG 3 cut(s) 31, 105, 339
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 4 cut(s) 56, 161, 205, 317
HapII CCGG 2 cut(s) 146, 206
Hin1II CATG 3 cut(s) 35, 109, 343
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 1 cut(s) 89
HpaII CCGG 2 cut(s) 146, 206
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 142, 183
Hpy8I GTNNAC 2 cut(s) 142, 183
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 2 cut(s) 127, 199
HpyCH4IV ACGT 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 81, 323
HpyF3I CTNAG 2 cut(s) 167, 303
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 3 cut(s) 35, 109, 343
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
Kzo9I GATC 3 cut(s) 129, 189, 292
LpnPI CCDG 6 cut(s) 66, 85, 159, 219, 303, 309
MaeII ACGT 1 cut(s) 138
MalI GATC 3 cut(s) 131, 191, 294
MboI GATC 3 cut(s) 129, 189, 292
MboII GAAGA 7 cut(s) 16, 127, 183, 227, 284, 295, 383
MfeI CAATTG 1 cut(s) 93
MflI RGATCY 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 3 cut(s) 20, 93, 382
MluNI TGGCCA 1 cut(s) 161
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 2 cut(s) 167, 236
Mox20I TGGCCA 1 cut(s) 161
MroNI GCCGGC 1 cut(s) 205
MscI TGGCCA 1 cut(s) 161
MseI TTAA 1 cut(s) 400
Msp20I TGGCCA 1 cut(s) 161
MspI CCGG 2 cut(s) 146, 206
MspR9I CCNGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 62, 81, 323
NaeI GCCGGC 1 cut(s) 207
NciI CCSGG 1 cut(s) 146
NdeII GATC 3 cut(s) 129, 189, 292
NgoMIV GCCGGC 1 cut(s) 205
NlaIII CATG 3 cut(s) 35, 109, 343
NlaIV GGNNCC 1 cut(s) 250
PceI AGGCCT 2 cut(s) 56, 317
PdiI GCCGGC 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 146
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 189
PsyI GACNNNGTC 1 cut(s) 146
RsaI GTAC 3 cut(s) 15, 182, 350
RsaNI GTAC 3 cut(s) 14, 181, 349
SaqAI TTAA 1 cut(s) 400
Sau3AI GATC 3 cut(s) 129, 189, 292
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 146
SetI ASST 4 cut(s) 12, 141, 292, 309
Sse9I AATT 3 cut(s) 20, 93, 382
SseBI AGGCCT 2 cut(s) 56, 317
StuI AGGCCT 2 cut(s) 56, 317
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 127, 199
TaiI ACGT 1 cut(s) 141
TaqI TCGA 1 cut(s) 150
TasI AATT 3 cut(s) 20, 93, 382
TatI WGTACW 1 cut(s) 348
Tru1I TTAA 1 cut(s) 400
Tru9I TTAA 1 cut(s) 400
TspDTI ATGAA 2 cut(s) 17, 122
TspGWI ACGGA 1 cut(s) 255
Tth111I GACNNNGTC 1 cut(s) 146
XapI RAATTY 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.