MD06G1090200.v1.1

late blight resistance protein homolog R1B-19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000998
Physical Location & Seq
Forward (+)
21910602 .. 21912173
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1090200.v1.1.491

Sequence Viewer

Length: 405 bp
ATGAAGAAAGTTGTACTGAAATTGGATTTACACGATGACAAGGAAAAGCAAAAAGCTTTGAAAGCAGTCTCTAGCCTTTCAGGCATTGACTCAATTGCTATGGACATGAAGGAGAGGAAACTAACAGTGATCGGCGACGTAGACCCGGTGGATGTGGTGGCCAAACTGAGAAAAGGGTGGTACACAGACATTCTAACAGTTGGGCCAGCAAAGGAAGAAAAGAAAGATGATAAGAAAGATGGAGGGGCTGCCAAAAAAGATGAAGGCAAGAAAGAAGATGATAAGAAGAAAGCTGATCCAGTTGCTGAACTTGTCAAAGCCTACCAAGCATACAATCCTCACATGACCAAGTACTACTATGTCCAAAGTGCTGAAGAAAACCCTAATTCCTGCGTTATCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

135

Amino Acids

14.97

Weight (kDa)

8.35

Isoelectric Point (pI)

23.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 9 - 61 1.5e-10 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 141
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 3 cut(s) 15, 182, 353
AgsI TTSAA 1 cut(s) 61
AluBI AGCT 2 cut(s) 56, 293
AluI AGCT 2 cut(s) 56, 293
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 2 cut(s) 159, 203
ApeKI GCWGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 146
BalI TGGCCA 1 cut(s) 161
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 233
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 72
BglI GCCNNNNNGGC 1 cut(s) 81
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 299
BseGI GGATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 2 cut(s) 161, 205
BsiSI CCGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 2 cut(s) 161, 205
Bsp143I GATC 2 cut(s) 129, 295
BspANI GGCC 2 cut(s) 161, 205
BspCNI CTCAG 1 cut(s) 159
BspPI GGATC 1 cut(s) 290
BsrI ACTGG 1 cut(s) 299
BssMI GATC 2 cut(s) 129, 295
Bst4CI ACNGT 2 cut(s) 127, 199
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 2 cut(s) 132, 298
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 129, 295
BstMWI GCNNNNNNNGC 3 cut(s) 62, 81, 326
BstSCI CCNGG 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 235
BsuRI GGCC 2 cut(s) 161, 205
BtsCI GGATG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 207
CaiI CAGNNNCTG 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 3 cut(s) 14, 181, 352
CviAII CATG 2 cut(s) 106, 343
CviJI RGCY 7 cut(s) 56, 75, 161, 205, 248, 293, 320
CviKI_1 RGCY 7 cut(s) 56, 75, 161, 205, 248, 293, 320
CviQI GTAC 3 cut(s) 14, 181, 352
DdeI CTNAG 1 cut(s) 167
DpnI GATC 2 cut(s) 131, 297
DpnII GATC 2 cut(s) 129, 295
EaeI YGGCCR 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 393
FaeI CATG 2 cut(s) 109, 346
FaiI YATR 5 cut(s) 101, 107, 331, 344, 360
FatI CATG 2 cut(s) 105, 342
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 1 cut(s) 72
GluI GCNGC 1 cut(s) 249
HaeIII GGCC 2 cut(s) 161, 205
HapII CCGG 1 cut(s) 146
Hin1II CATG 2 cut(s) 109, 346
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 1 cut(s) 89
HpaII CCGG 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 142, 183
Hpy8I GTNNAC 2 cut(s) 142, 183
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 103, 257
HpyCH4III ACNGT 2 cut(s) 127, 199
HpyCH4IV ACGT 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 81, 326
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 2 cut(s) 109, 346
Kzo9I GATC 2 cut(s) 129, 295
LpnPI CCDG 4 cut(s) 66, 159, 219, 312
Lsp1109I GCAGC 1 cut(s) 235
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 138
MalI GATC 2 cut(s) 131, 297
MboI GATC 2 cut(s) 129, 295
MboII GAAGA 5 cut(s) 16, 227, 287, 298, 386
MfeI CAATTG 1 cut(s) 93
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 3 cut(s) 20, 93, 385
MluNI TGGCCA 1 cut(s) 161
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 3 cut(s) 108, 236, 348
Mox20I TGGCCA 1 cut(s) 161
MscI TGGCCA 1 cut(s) 161
MseI TTAA 1 cut(s) 403
Msp20I TGGCCA 1 cut(s) 161
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 62, 81, 326
NciI CCSGG 1 cut(s) 146
NdeII GATC 2 cut(s) 129, 295
NlaIII CATG 2 cut(s) 109, 346
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 305
RsaI GTAC 3 cut(s) 15, 182, 353
RsaNI GTAC 3 cut(s) 14, 181, 352
SaqAI TTAA 1 cut(s) 403
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 2 cut(s) 129, 295
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 146
SetI ASST 3 cut(s) 58, 141, 295
Sse9I AATT 3 cut(s) 20, 93, 385
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 127, 199
TaiI ACGT 1 cut(s) 141
TasI AATT 3 cut(s) 20, 93, 385
TatI WGTACW 2 cut(s) 13, 351
Tru1I TTAA 1 cut(s) 403
Tru9I TTAA 1 cut(s) 403
TscAI CASTG 1 cut(s) 132
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 3 cut(s) 17, 122, 276
TspRI CASTG 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.