RLG00000004001

late blight resistance protein homolog R1B-19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000998
Physical Location & Seq
Forward (+)
55149095 .. 55149934
840 bp
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UTR
Exon/CDS
Intron
RLM00000004001

Sequence Viewer

Length: 399 bp
ATGAAGAAGGTGGTATTGAAACTTGATTTGCACGATGACAGGGAGAAGCAGAAGGCGTTGAAAGCAGTGTCTGGTCTTTCTGGCATTGATTCCATTGCTATGGACATGAAAGAGAGGAAGTTAACTGTGACTGGCAATATTGATCCGGTTCATGTGGTGAACAAGTTGCGGAAAGGTAACTGGCACACTGAGATTTTGACAGTTGGGCCGGCGAAAGAAGAGAAGAAGGATGATAAGAAAGACGGAGGAGCTAAGAAAGATGATGGCAAGAAAGAAGATGACAACAAGAAGCAAATTGCTGAGCTTGTGCGAGCATATCAAAACTATAATCCGCAGATGACGAGGTACTATTATGTGCAGAGCGCAGAAGAAAACCCAAATGCTTGTGTTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

133

Amino Acids

14.96

Weight (kDa)

9.03

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 9 - 57 2e-08 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 169, 332
AclWI GGATC 1 cut(s) 137
AfaI GTAC 1 cut(s) 347
AgsI TTSAA 2 cut(s) 19, 61
AluBI AGCT 2 cut(s) 251, 304
AluI AGCT 2 cut(s) 251, 304
AlwI GGATC 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 169
BccI CCATC 1 cut(s) 257
BlpI GCTNAGC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 145
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 136, 185
Bse3DI GCAATG 1 cut(s) 93
BseGI GGATG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 2 cut(s) 180, 291
BseNI ACTGG 2 cut(s) 136, 185
BseRI GAGGAG 1 cut(s) 261
BsgI GTGCAG 1 cut(s) 377
BshFI GGCC 1 cut(s) 208
BsiSI CCGG 2 cut(s) 146, 209
BsnI GGCC 1 cut(s) 208
Bsp143I GATC 1 cut(s) 142
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 2 cut(s) 169, 332
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 2 cut(s) 181, 292
BspPI GGATC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 2 cut(s) 136, 185
BssAI RCCGGY 1 cut(s) 208
BssMI GATC 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 127, 202
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 210, 312
BstDEI CTNAG 3 cut(s) 189, 252, 300
BstF5I GGATG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 100
BsuRI GGCC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 186
Cac8I GCNNGC 2 cut(s) 210, 312
CaiI CAGNNNCTG 1 cut(s) 71
CfoI GCGC 1 cut(s) 365
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 346
CviAII CATG 2 cut(s) 106, 152
CviJI RGCY 3 cut(s) 208, 251, 304
CviKI_1 RGCY 3 cut(s) 208, 251, 304
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 3 cut(s) 189, 252, 300
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
FaeI CATG 2 cut(s) 109, 155
FaiI YATR 6 cut(s) 101, 107, 153, 316, 327, 354
FatI CATG 2 cut(s) 105, 151
FokI GGATG 1 cut(s) 242
GlaI GCGC 1 cut(s) 364
HaeIII GGCC 1 cut(s) 208
HapII CCGG 2 cut(s) 146, 209
HhaI GCGC 1 cut(s) 365
Hin1II CATG 2 cut(s) 109, 155
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 1 cut(s) 89
HpaI GTTAAC 1 cut(s) 123
HpaII CCGG 2 cut(s) 146, 209
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 123, 160
Hpy8I GTNNAC 2 cut(s) 123, 160
HpyAV CCTTC 2 cut(s) 46, 220
HpyCH4III ACNGT 2 cut(s) 127, 202
HpyCH4V TGCA 2 cut(s) 31, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 3 cut(s) 189, 252, 300
Hsp92II CATG 2 cut(s) 109, 155
HspAI GCGC 1 cut(s) 363
KroI GCCGGC 1 cut(s) 208
KroNI GCCGGC 1 cut(s) 210
KspAI GTTAAC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 142
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 7 cut(s) 25, 57, 66, 117, 159, 166, 222
MaeIII GTNAC 2 cut(s) 127, 176
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 5 cut(s) 16, 230, 235, 287, 380
MluCI AATT 1 cut(s) 294
MnlI CCTC 3 cut(s) 108, 239, 336
MroNI GCCGGC 1 cut(s) 208
MseI TTAA 2 cut(s) 122, 397
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 2 cut(s) 146, 209
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 1 cut(s) 210
NdeII GATC 1 cut(s) 142
NgoMIV GCCGGC 1 cut(s) 208
NlaIII CATG 2 cut(s) 109, 155
NmuCI GTSAC 1 cut(s) 127
PdiI GCCGGC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 206
PstNI CAGNNNCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 2 cut(s) 122, 397
Sau3AI GATC 1 cut(s) 142
Sau96I GGNCC 1 cut(s) 206
SetI ASST 5 cut(s) 12, 178, 253, 306, 347
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 1 cut(s) 294
SsiI CCGC 2 cut(s) 169, 332
SspI AATATT 1 cut(s) 139
TaaI ACNGT 2 cut(s) 127, 202
TasI AATT 1 cut(s) 294
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 122, 397
Tru9I TTAA 2 cut(s) 122, 397
TscAI CASTG 2 cut(s) 72, 193
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 17, 122, 140
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 72, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.