MD01G1209200.v1.1

FK506-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000998
Physical Location & Seq
Forward (+)
30342951 .. 30344108
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1209200.v1.1.491

Sequence Viewer

Length: 381 bp
ATGAAGAAAGTAGTGTTGAAATTGGAATTACACGACGAGAAGGGTAAGAAGAAAGCCATGAGGGCCGTCTCGGGGCTTGAAGGGCTTGATTCAATTTCTATGGATATGAAGGACAAGAAACTGACGGTCACAGGGGACATAGATCCAGTGGACTTGGTGGGAAGATTGAGGAAGCTTTGCCGGGCGGAGATAGTCTCGGTCGGACCGGCAAAGGAGGAGAAGAAGAAAGAGGAGCCAAAGAAGGAGGAGACGAAGCCGAAAGATCCAAAGGACCAAATGGCTGAGCTTATCAAGGCCTACCAAGCTTATTGTCCTCCAATGCCTGCATATTATTACGTAAAAAGTTCAGAAGAGGATCCCAGTGCATGTGTCATTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

127

Amino Acids

14.16

Weight (kDa)

8.56

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 10 - 58 7.3e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 185
AclWI GGATC 4 cut(s) 137, 257, 350, 363
AfiI CCNNNNNNNGG 1 cut(s) 72
AgsI TTSAA 3 cut(s) 19, 80, 93
AluBI AGCT 3 cut(s) 175, 286, 305
AluI AGCT 3 cut(s) 175, 286, 305
Alw26I GTCTC 3 cut(s) 73, 199, 242
AlwI GGATC 4 cut(s) 137, 257, 350, 363
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 2 cut(s) 63, 294
AspS9I GGNCC 3 cut(s) 63, 203, 271
AsuC2I CCSGG 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 2 cut(s) 203, 271
BamHI GGATCC 1 cut(s) 355
BceAI ACGGC 1 cut(s) 50
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 3 cut(s) 73, 199, 242
BglI GCCNNNNNGGC 1 cut(s) 62
BlpI GCTNAGC 1 cut(s) 282
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 2 cut(s) 203, 271
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 63, 203, 271
BmiI GGNNCC 2 cut(s) 234, 357
BmrFI CCNGG 1 cut(s) 182
BmrI ACTGGG 1 cut(s) 354
BmuI ACTGGG 1 cut(s) 354
BplI GAGNNNNNCTC 2 cut(s) 179, 211
Bpu1102I GCTNAGC 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 182
BsaAI YACGTR 1 cut(s) 337
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 2 cut(s) 146, 360
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 2 cut(s) 146, 360
BseRI GAGGAG 3 cut(s) 230, 245, 260
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 65, 296
BsiEI CGRYCG 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 2 cut(s) 181, 206
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 3 cut(s) 73, 199, 242
BsmBI CGTCTC 2 cut(s) 73, 242
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 2 cut(s) 65, 296
BsoBI CYCGRG 1 cut(s) 70
Bsp143I GATC 3 cut(s) 142, 262, 355
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 1 cut(s) 185
BspANI GGCC 2 cut(s) 65, 296
BspCNI CTCAG 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 234, 357
BspPI GGATC 4 cut(s) 137, 257, 350, 363
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 2 cut(s) 146, 360
BssAI RCCGGY 1 cut(s) 205
BssMI GATC 3 cut(s) 142, 262, 355
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 282
BstKTI GATC 3 cut(s) 145, 265, 358
BstMAI GTCTC 3 cut(s) 73, 199, 242
BstMBI GATC 3 cut(s) 142, 262, 355
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 3 cut(s) 62, 82, 302
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 180
BstSNI TACGTA 1 cut(s) 337
BstX2I RGATCY 3 cut(s) 142, 262, 355
BstYI RGATCY 3 cut(s) 142, 262, 355
BsuRI GGCC 2 cut(s) 65, 296
BtsIMutI CAGTG 2 cut(s) 153, 367
Cac8I GCNNGC 1 cut(s) 324
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 63, 203, 271
CpoI CGGWCCG 1 cut(s) 203
CspI CGGWCCG 1 cut(s) 203
CviAII CATG 2 cut(s) 58, 366
DdeI CTNAG 1 cut(s) 282
DpnI GATC 3 cut(s) 144, 264, 357
DpnII GATC 3 cut(s) 142, 262, 355
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
EciI GGCGGA 1 cut(s) 200
Eco105I TACGTA 1 cut(s) 337
Eco147I AGGCCT 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 203, 271
Eco88I CYCGRG 1 cut(s) 70
Esp3I CGTCTC 2 cut(s) 73, 242
FaeI CATG 2 cut(s) 61, 369
FaiI YATR 6 cut(s) 59, 101, 107, 140, 328, 367
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 57, 365
HaeIII GGCC 2 cut(s) 65, 296
HapII CCGG 2 cut(s) 181, 206
Hin1II CATG 2 cut(s) 61, 369
HindIII AAGCTT 2 cut(s) 173, 303
HinfI GANTC 1 cut(s) 89
HpaII CCGG 2 cut(s) 181, 206
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 203, 349
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 34, 74, 103, 235
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 2 cut(s) 326, 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 82, 302
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 2 cut(s) 61, 369
Kzo9I GATC 3 cut(s) 142, 262, 355
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 6 cut(s) 117, 159, 194, 219, 336, 373
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 127
MalI GATC 3 cut(s) 144, 264, 357
MboI GATC 3 cut(s) 142, 262, 355
MboII GAAGA 6 cut(s) 16, 61, 174, 232, 235, 362
MflI RGATCY 3 cut(s) 142, 262, 355
MluCI AATT 3 cut(s) 20, 26, 93
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 7 cut(s) 54, 162, 208, 223, 238, 324, 346
MspI CCGG 2 cut(s) 181, 206
MspR9I CCNGG 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 62, 82, 302
NciI CCSGG 1 cut(s) 182
NdeII GATC 3 cut(s) 142, 262, 355
NlaIII CATG 2 cut(s) 61, 369
NlaIV GGNNCC 2 cut(s) 234, 357
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 369
PceI AGGCCT 1 cut(s) 296
PfeI GAWTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 234, 357
PspPI GGNCC 3 cut(s) 63, 203, 271
PsuI RGATCY 3 cut(s) 142, 262, 355
Rsr2I CGGWCCG 1 cut(s) 203
RsrII CGGWCCG 1 cut(s) 203
Sau3AI GATC 3 cut(s) 142, 262, 355
Sau96I GGNCC 3 cut(s) 63, 203, 271
ScrFI CCNGG 1 cut(s) 182
SetI ASST 4 cut(s) 177, 288, 307, 339
SinI GGWCC 2 cut(s) 203, 271
SnaBI TACGTA 1 cut(s) 337
Sse9I AATT 3 cut(s) 20, 26, 93
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 1 cut(s) 185
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 339
TaqII GACCGA 1 cut(s) 187
TasI AATT 3 cut(s) 20, 26, 93
TfiI GAWTC 1 cut(s) 89
TscAI CASTG 2 cut(s) 153, 367
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 17, 122
TspRI CASTG 2 cut(s) 153, 367
VpaK11BI GGWCC 2 cut(s) 203, 271
XceI RCATGY 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.