Prupe.2G176600_v2.0.a1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000998
Physical Location & Seq
Forward (+)
22192242 .. 22194840
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G176600.2

Sequence Viewer

Length: 498 bp
ATGAAGAAGTTTGTACTGAAGTTGGATTTGCATGATGACAGAGCCAAGCAGAAGGCCCTGAAAACAGTCTCTACTCTTTCAGGTATTGATTCCATCTCCATGGACATGAAGGAGAAGAAATTAACGGTGATCGGAAGTGTCGACCCGGTGACTGTGGTGAGCAAATTGCGCAAGTATTGGCAAACAGATATACTCTCAGTAGGACCAGCAGTAGAGCCTAAGAAAGAGGAACCAAAGAAGGAAGAACCCAAGAAAGAAGAAGAAGCAAAGAAGGAGGAACCCAAGAAAGAAGAAGCAAAGAAGGAGGAACCCAAGAAAGAAGAAGCAAAGAAGGAAGAGGAACCAAAGAAGGAGGAGCCAAAGAAGGAGGAGCCAAAGAAAGAGCCAGATCCTGTCTTAGAGCTTGTTAAGGCTTACAAAGCATACAACCCCCATCTGACCACATATTACTATGTGCAAAGCATGGAAGAGAATCCAAATGCTTGTGTTATTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

166

Amino Acids

19.07

Weight (kDa)

7.63

Isoelectric Point (pI)

53.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 170
AccI GTMKAC 1 cut(s) 141
AclWI GGATC 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 1 cut(s) 15
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 55, 203
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 139, 160, 169
AvaII GGWCC 1 cut(s) 203
BccI CCATC 2 cut(s) 101, 441
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 73
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 55, 203
BmiI GGNNCC 6 cut(s) 231, 279, 309, 342, 357, 372
BmrFI CCNGG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 210
BseRI GAGGAG 2 cut(s) 368, 383
BshFI GGCC 1 cut(s) 56
BsiSI CCGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 1 cut(s) 56
Bsp143I GATC 2 cut(s) 129, 388
Bsp19I CCATGG 1 cut(s) 99
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 209
BspLI GGNNCC 6 cut(s) 231, 279, 309, 342, 357, 372
BspPI GGATC 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 129, 388
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 3 cut(s) 67, 127, 154
Bst6I CTCTTC 2 cut(s) 330, 462
BstDEI CTNAG 3 cut(s) 196, 219, 397
BstDSI CCRYGG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 132, 391
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 129, 388
BstMWI GCNNNNNNNGC 2 cut(s) 168, 419
BstSCI CCNGG 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 99
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 55, 203
Csp6I GTAC 1 cut(s) 14
CviAII CATG 4 cut(s) 32, 100, 106, 463
CviJI RGCY 8 cut(s) 44, 56, 217, 358, 373, 385, 403, 413
CviKI_1 RGCY 8 cut(s) 44, 56, 217, 358, 373, 385, 403, 413
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 3 cut(s) 196, 219, 397
DpnI GATC 2 cut(s) 131, 390
DpnII GATC 2 cut(s) 129, 388
Eam1104I CTCTTC 2 cut(s) 330, 462
EarI CTCTTC 2 cut(s) 330, 462
Eco130I CCWWGG 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 4 cut(s) 35, 103, 109, 466
FaiI YATR 8 cut(s) 33, 101, 107, 191, 424, 445, 453, 464
FatI CATG 4 cut(s) 31, 99, 105, 462
FblI GTMKAC 1 cut(s) 141
FspI TGCGCA 1 cut(s) 170
GlaI GCGC 1 cut(s) 170
HaeIII GGCC 1 cut(s) 56
HapII CCGG 1 cut(s) 146
HhaI GCGC 1 cut(s) 171
Hin1II CATG 4 cut(s) 35, 103, 109, 466
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 2 cut(s) 89, 472
HpaII CCGG 1 cut(s) 146
HphI GGTGA 3 cut(s) 139, 160, 169
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 2 cut(s) 134, 438
Hpy8I GTNNAC 1 cut(s) 142
HpyAV CCTTC 8 cut(s) 46, 103, 232, 265, 295, 325, 343, 358
HpyCH4III ACNGT 3 cut(s) 67, 127, 154
HpyCH4V TGCA 2 cut(s) 31, 457
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 419
HpyF3I CTNAG 3 cut(s) 196, 219, 397
Hsp92II CATG 4 cut(s) 35, 103, 109, 466
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 129, 388
LmnI GCTCC 2 cut(s) 355, 370
LpnPI CCDG 6 cut(s) 66, 71, 159, 219, 399, 405
MaeIII GTNAC 1 cut(s) 148
MalI GATC 2 cut(s) 131, 390
MboI GATC 2 cut(s) 129, 388
MboII GAAGA 9 cut(s) 16, 127, 254, 269, 272, 302, 332, 347, 479
MflI RGATCY 1 cut(s) 388
MluCI AATT 2 cut(s) 119, 164
MnlI CCTC 6 cut(s) 220, 268, 298, 331, 346, 361
MseI TTAA 2 cut(s) 122, 408
MslI CAYNNNNRTG 2 cut(s) 98, 104
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 146
MwoI GCNNNNNNNGC 2 cut(s) 168, 419
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 1 cut(s) 99
NdeII GATC 2 cut(s) 129, 388
NlaIII CATG 4 cut(s) 35, 103, 109, 466
NlaIV GGNNCC 6 cut(s) 231, 279, 309, 342, 357, 372
NmuCI GTSAC 1 cut(s) 148
NsbI TGCGCA 1 cut(s) 170
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 2 cut(s) 89, 472
PspN4I GGNNCC 6 cut(s) 231, 279, 309, 342, 357, 372
PspPI GGNCC 2 cut(s) 55, 203
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
RseI CAYNNNNRTG 2 cut(s) 98, 104
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 2 cut(s) 122, 408
Sau3AI GATC 2 cut(s) 129, 388
Sau96I GGNCC 2 cut(s) 55, 203
ScrFI CCNGG 1 cut(s) 146
SetI ASST 2 cut(s) 85, 405
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 98, 104
Sse9I AATT 2 cut(s) 119, 164
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 3 cut(s) 67, 127, 154
TaqI TCGA 1 cut(s) 141
TasI AATT 2 cut(s) 119, 164
TatI WGTACW 1 cut(s) 13
TfiI GAWTC 2 cut(s) 89, 472
Tru1I TTAA 2 cut(s) 122, 408
Tru9I TTAA 2 cut(s) 122, 408
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 17, 122
VpaK11BI GGWCC 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.