RchiOBHm_Chr1g0378461

FK506-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000998
Physical Location & Seq
Forward (+)
64952174 .. 64953510
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60188

Sequence Viewer

Length: 393 bp
ATGAAGAAAGTTGTGTTGTTGTTGGACGTACACGACGACAGGTCCAAGAAGAAAGCCATGAGGGCAGTCTCAGGGCTCGAAGGGCTAGATTCGATCTCGATGGACATGAAGGACAAGAAGCTCACGGTGACTGGGGAAATAGATCCAGTGAACTTGGTGAGCAAGTTGAGGAAGCTATGCCGCACTGAGATAGTGTCGGTAGGACCAGCAAAAGAGGAAAAGAAGAAGGAAGAGAAGAAAGAGGAACCCAAAAAGGGTGGGGAGAAGAAGAAAGATCCAAAAGATGAACTGGCTGAGCTAATCAAGGCCTACAATGCATACAACCCACCAATGAGTACGCATTATTATGTGAGAAGTGTGGAAGAGGATCCCAATGCCTGTGTCATCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

130

Amino Acids

14.67

Weight (kDa)

8.85

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 9 - 58 3.4e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 181
AclWI GGATC 4 cut(s) 137, 269, 362, 375
AfaI GTAC 2 cut(s) 30, 337
AfiI CCNNNNNNNGG 1 cut(s) 254
AhdI GACNNNNNGTC 1 cut(s) 40
AluBI AGCT 3 cut(s) 121, 175, 298
AluI AGCT 3 cut(s) 121, 175, 298
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 4 cut(s) 137, 269, 362, 375
AoxI GGCC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 42, 203
AsuHPI GGTGA 2 cut(s) 139, 169
AvaII GGWCC 2 cut(s) 42, 203
BamHI GGATCC 1 cut(s) 367
BanII GRGCYC 1 cut(s) 78
BccI CCATC 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 86
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 1 cut(s) 181
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 182
Bme18I GGWCC 2 cut(s) 42, 203
BmeRI GACNNNNNGTC 1 cut(s) 40
BmgT120I GGNCC 2 cut(s) 42, 203
BmiI GGNNCC 2 cut(s) 246, 369
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 294
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 3 cut(s) 136, 146, 294
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 3 cut(s) 84, 177, 285
BseNI ACTGG 3 cut(s) 136, 146, 294
BshFI GGCC 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 4 cut(s) 93, 142, 274, 367
Bsp1720I GCTNAGC 1 cut(s) 294
BspACI CCGC 1 cut(s) 181
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 3 cut(s) 83, 178, 286
BspLI GGNNCC 2 cut(s) 246, 369
BspPI GGATC 4 cut(s) 137, 269, 362, 375
BsrI ACTGG 3 cut(s) 136, 146, 294
BssMI GATC 4 cut(s) 93, 142, 274, 367
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 2 cut(s) 225, 357
BstDEI CTNAG 3 cut(s) 70, 186, 294
BstKTI GATC 4 cut(s) 96, 145, 277, 370
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 4 cut(s) 93, 142, 274, 367
BstMWI GCNNNNNNNGC 3 cut(s) 62, 82, 314
BstX2I RGATCY 3 cut(s) 142, 274, 367
BstYI RGATCY 3 cut(s) 142, 274, 367
BsuRI GGCC 1 cut(s) 308
BtsIMutI CAGTG 2 cut(s) 153, 183
Cfr13I GGNCC 2 cut(s) 42, 203
Csp6I GTAC 2 cut(s) 29, 336
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 2 cut(s) 58, 106
CviJI RGCY 8 cut(s) 56, 76, 85, 121, 175, 293, 298, 308
CviKI_1 RGCY 8 cut(s) 56, 76, 85, 121, 175, 293, 298, 308
CviQI GTAC 2 cut(s) 29, 336
DdeI CTNAG 3 cut(s) 70, 186, 294
DpnI GATC 4 cut(s) 95, 144, 276, 369
DpnII GATC 4 cut(s) 93, 142, 274, 367
DriI GACNNNNNGTC 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 225, 357
Eam1105I GACNNNNNGTC 1 cut(s) 40
EarI CTCTTC 2 cut(s) 225, 357
Eco147I AGGCCT 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 2 cut(s) 42, 203
EcoT22I ATGCAT 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 78
FaeI CATG 2 cut(s) 61, 109
FaiI YATR 5 cut(s) 59, 107, 178, 319, 348
FatI CATG 2 cut(s) 57, 105
Fnu4HI GCNGC 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 1 cut(s) 86
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 308
Hin1II CATG 2 cut(s) 61, 109
HinfI GANTC 1 cut(s) 89
HphI GGTGA 2 cut(s) 139, 169
Hpy166II GTNNAC 2 cut(s) 31, 151
Hpy188III TCNNGA 1 cut(s) 97
Hpy8I GTNNAC 2 cut(s) 31, 151
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 3 cut(s) 74, 103, 220
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 82, 314
HpyF3I CTNAG 3 cut(s) 70, 186, 294
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 61, 109
Kzo9I GATC 4 cut(s) 93, 142, 274, 367
LpnPI CCDG 6 cut(s) 25, 57, 117, 159, 219, 275
MaeI CTAG 1 cut(s) 86
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 127
MalI GATC 4 cut(s) 95, 144, 276, 369
MboI GATC 4 cut(s) 93, 142, 274, 367
MboII GAAGA 8 cut(s) 16, 61, 235, 242, 247, 277, 280, 374
MflI RGATCY 3 cut(s) 142, 274, 367
MhlI GDGCHC 1 cut(s) 78
MnlI CCTC 5 cut(s) 54, 162, 208, 235, 358
Mph1103I ATGCAT 1 cut(s) 319
MslI CAYNNNNRTG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 62, 82, 314
NdeII GATC 4 cut(s) 93, 142, 274, 367
NlaIII CATG 2 cut(s) 61, 109
NlaIV GGNNCC 2 cut(s) 246, 369
NmuCI GTSAC 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 319
PceI AGGCCT 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 246, 369
PspPI GGNCC 2 cut(s) 42, 203
PsuI RGATCY 3 cut(s) 142, 274, 367
RsaI GTAC 2 cut(s) 30, 337
RsaNI GTAC 2 cut(s) 29, 336
RseI CAYNNNNRTG 1 cut(s) 345
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 4 cut(s) 93, 142, 274, 367
Sau96I GGNCC 2 cut(s) 42, 203
SduI GDGCHC 1 cut(s) 78
SetI ASST 5 cut(s) 30, 44, 123, 177, 300
SinI GGWCC 2 cut(s) 42, 203
SmiMI CAYNNNNRTG 1 cut(s) 345
SseBI AGGCCT 1 cut(s) 308
SsiI CCGC 1 cut(s) 181
SspMI CTAG 1 cut(s) 86
StuI AGGCCT 1 cut(s) 308
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 78, 92, 98
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 89
TscAI CASTG 2 cut(s) 153, 190
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 17, 122, 300
TspRI CASTG 2 cut(s) 153, 190
VpaK11BI GGWCC 2 cut(s) 42, 203
XspI CTAG 1 cut(s) 86
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.