Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001129

Physical Location & Seq

Genomic Coordinates Reverse (-)

17344354 .. 17349647

Gene Structure 5294 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1069400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGGCGTTCCCGTTCTTGGAAGCTGTTATCGGTTTTATGATATCATTGTACTTTTTCGAGACTTATTTGGATTTGCGGCAACATGCTGCTCACAAACTTCCAACCCTTCCTAAAACTTTGGAAGGAGTAATCAGCCAAGAAAAGTTTGAGAAGTCACGAGCCTATAGTCTTGATAAAAGCCGCTTCCATTTTGTTCATGAGTTTGTGACAATACTGATGGACTCAGCAATTTTGTTCTTTCGAGTGTTGCCTTGGTTTTGGAAGAGATCAGCAGATTTCGTGGCTTTAGCTGGTCTCAATGCTGAAAATGAAATCTGGCATACACTTGCATTTTTGGGTGGTGTTATGATTTGGTCACAGATCACTGATTTACCTTTTTCTCTATACTCCACATTTGTGGTTGAAGCTCGTCATGGTTTTAATAAGCAAACAATATGGTTATTCTTCAGAGACATGATTAAAGGAATTTGCCTCACTGTTATTCTCGGCCCACCTATTGTGTCTGCAATAATTGTAATTGTACAGAAAGGAGGTGATTACCTGGCCATCTATCTTTGGGCATTCATGTTTGTTCTCTCTCTTGTGATGATGACACTCTACCCTGTTCTAATAGCCCCTCTCTTTAACAAGTTCACCCCTCTTCCAGAGGGTGAGCTCAGGCAGAAAATTGAGAAACTTGCTTCTTCTCTCAAGTTTCCATTGAAGAAGTTGTTTGTCGTTGATGGATCTACAAGGTCAAGTCACAGCAATGCCTATATGTATGGATTTTTTAAGAACAAGAGAATCGTCCTCTATGATACATTAATTCAACAGTGCAAAAATGATGAGGAGATTGTAGCTGTTATAGCTCATGAGTTAGGCCATTGGAAGCTCAACCACACACTGTATTCATTTATTGCTGTGCAGATCCTTACATTTTTACAGTTTGGAGGATATACTCTAGTGAGAAACTCAAGCAGTCTGTTTCAAAGTTTTGGGTTTGATACTCAGCCAGTAATCATTGGACTCATCATATTTCAGCACACCATAATACCTATCCAGCACATAGTAAGCTTTGCTCTTAACCTTGTGAGCCGATCATTTGAATTTCAGGCTGATGCTTTTGCTAAGAAGCTTGGTTATTCGTCTGCACTTCGAGCTGGTCTTGTTAAACTACAGGAGGAGAATTTGTCGGCTATGAACACTGATCCTTGGTACTCTGCATATCACTATTCTCATCCTCCACTTGTCGAAAGGCTGGCTGCGATTGATAAACCAGACAAGAAAGCAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005789 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0051604 GO:0070011 GO:0071586 GO:0071704 GO:0080120 GO:0098827 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00900 ko01130 map00900 map01130

Pfam Domains

Protein Families

Peptidase_M48 Peptidase_M48_N

Protein Analysis

425

Amino Acids

48.55

Weight (kDa)

8.77

Isoelectric Point (pI)

39.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M48_N	PF16491	30 - 210	1.1e-66	CAAX prenyl protease N-terminal, five membrane helices
Peptidase_M48	PF01435	213 - 419	1.2e-50	Peptidase family M48

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001129)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01320 AT4G01320 AT4G01320
fragaria_vesca	FvH4_7g15081 FvH4_7g15081 FvH4_7g15081 FvH4_7g15083
malus_domestica	MD01G1069400.v1.1
prunus_persica	Prupe.2G175500_v2.0.a1 Prupe.2G175500_v2.0.a1 Prupe.2G175500_v2.0.a1
pyrus_communis	pycom01g09880
rosa_chinensis	RchiOBHm_Chr1g0357201 RchiOBHm_Chr1g0357211 RchiOBHm_Chr1g0357271 RchiOBHm_Chr3g0493601
rosa_laevigata	RLG00000018289 RLG00000028032 RLG00000028038 RLG00000028039 RLG00000028042 RLG00000028044
rosa_multiflora	Rmu_sc0004075.1_g000007 Rmu_sc0004792.1_g000002 Rmu_sc0005967.1_g000005 Rmu_sc0006798.1_g000014 Rmu_sc0007965.1_g000003 Rmu_sc0017004.1_g000001
rosa_roxburghii	Rroxscaffold_4G00298940 Rroxscaffold_4G00298980 Rroxscaffold_4G00298990
rosa_rugosa	Rorug01G0213800 Rorug01G0254900 Rorug01G0255300 Rorug01G0255400 Rorug01G0255600 Rorug01G0255800 Rorug01G0255900 Rorug04G0302100 Rorug04G0302200 Rorug04G0302300 Rorug04G0302400
rosa_samantha	Rh1AG269100 Rh1AG269200 Rh1CG150800 Rh1CG252800 Rh1CG252900 Rh1CG253400 Rh1DG264200 Rh1DG264300 Rh3AG311700
rosa_wichuraiana	Rw1G023750 Rw1G023760 Rw1G023770 Rw1G023780 Rw1G023800 Rw1G023840 Rw1G024020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	76, 181
AclWI	GGATC	3 cut(s)	733, 901, 1181
AcoI	YGGCCR	1 cut(s)	543
AcsI	RAATTY	3 cut(s)	465, 1085, 1165
AcuI	CTGAAG	1 cut(s)	430
AfaI	GTAC	3 cut(s)	50, 522, 1196
AfiI	CCNNNNNNNGG	1 cut(s)	16
AgsI	TTSAA	5 cut(s)	404, 703, 809, 968, 1085
AjnI	CCWGG	1 cut(s)	540
AleI	CACNNNNGTG	1 cut(s)	395
Alw21I	GWGCWC	1 cut(s)	657
Alw26I	GTCTC	3 cut(s)	53, 299, 444
AlwI	GGATC	3 cut(s)	733, 901, 1181
AoxI	GGCC	3 cut(s)	487, 543, 859
ApeKI	GCWGC	2 cut(s)	86, 1241
ApoI	RAATTY	3 cut(s)	465, 1085, 1165
AseI	ATTAAT	1 cut(s)	803
AspS9I	GGNCC	1 cut(s)	488
AsuHPI	GGTGA	3 cut(s)	545, 625, 662
BalI	TGGCCA	1 cut(s)	545
BanII	GRGCYC	1 cut(s)	657
BauI	CACGAG	1 cut(s)	156
Bbv12I	GWGCWC	1 cut(s)	657
BbvI	GCAGC	2 cut(s)	73, 1228
BccI	CCATC	3 cut(s)	211, 554, 716
BciT130I	CCWGG	1 cut(s)	542
BcoDI	GTCTC	3 cut(s)	53, 299, 444
BfaI	CTAG	1 cut(s)	941
BfmI	CTRYAG	2 cut(s)	163, 1154
BisI	GCNGC	4 cut(s)	77, 87, 181, 1242
BlsI	GCNGC	4 cut(s)	78, 88, 182, 1243
Bme1390I	CCNGG	1 cut(s)	542
BmgT120I	GGNCC	1 cut(s)	488
BmrFI	CCNGG	1 cut(s)	542
BmsI	GCATC	1 cut(s)	1087
Bpu10I	CCTNAGC	1 cut(s)	656
BpuEI	CTTGAG	2 cut(s)	674, 937
BsaI	GGTCTC	1 cut(s)	299
BsaJI	CCNNGG	2 cut(s)	251, 1190
BsaXI	ACNNNNNCTCC	2 cut(s)	1154, 1184
Bsc4I	CCNNNNNNNGG	1 cut(s)	16
Bse1I	ACTGG	1 cut(s)	992
Bse3DI	GCAATG	1 cut(s)	754
BseBI	CCWGG	1 cut(s)	542
BseDI	CCNNGG	2 cut(s)	251, 1190
BseGI	GGATG	1 cut(s)	1216
BseLI	CCNNNNNNNGG	1 cut(s)	16
BseMI	GCAATG	1 cut(s)	754
BseMII	CTCAG	3 cut(s)	237, 670, 1001
BseNI	ACTGG	1 cut(s)	992
BseRI	GAGGAG	2 cut(s)	842, 1175
BseXI	GCAGC	2 cut(s)	73, 1228
BsgI	GTGCAG	2 cut(s)	923, 1113
BshFI	GGCC	3 cut(s)	489, 545, 861
BsiHKAI	GWGCWC	1 cut(s)	657
BslI	CCNNNNNNNGG	1 cut(s)	16
BsmAI	GTCTC	3 cut(s)	53, 299, 444
BsmI	GAATGC	1 cut(s)	560
BsnI	GGCC	3 cut(s)	489, 545, 861
Bso31I	GGTCTC	1 cut(s)	299
Bsp1286I	GDGCHC	1 cut(s)	657
Bsp1407I	TGTACA	1 cut(s)	520
Bsp143I	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
BspACI	CCGC	2 cut(s)	76, 181
BspANI	GGCC	3 cut(s)	489, 545, 861
BspCNI	CTCAG	3 cut(s)	236, 669, 1000
BspHI	TCATGA	2 cut(s)	196, 850
BspPI	GGATC	3 cut(s)	733, 901, 1181
BspTNI	GGTCTC	1 cut(s)	299
BsrDI	GCAATG	1 cut(s)	754
BsrGI	TGTACA	1 cut(s)	520
BsrI	ACTGG	1 cut(s)	992
BssECI	CCNNGG	2 cut(s)	251, 1190
BssMI	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
BssSI	CACGAG	1 cut(s)	156
BssT1I	CCWWGG	2 cut(s)	251, 1190
Bst2BI	CACGAG	1 cut(s)	156
Bst2UI	CCWGG	1 cut(s)	542
Bst4CI	ACNGT	4 cut(s)	478, 813, 885, 924
Bst6I	CTCTTC	2 cut(s)	257, 645
BstAUI	TGTACA	1 cut(s)	520
BstC8I	GCNNGC	1 cut(s)	1239
BstDEI	CTNAG	4 cut(s)	223, 656, 987, 1107
BstF5I	GGATG	1 cut(s)	1216
BstKTI	GATC	6 cut(s)	269, 363, 728, 909, 1079, 1189
BstMAI	GTCTC	3 cut(s)	53, 299, 444
BstMBI	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
BstMWI	GCNNNNNNNGC	2 cut(s)	845, 1136
BstNI	CCWGG	1 cut(s)	542
BstNSI	RCATGY	1 cut(s)	86
BstSCI	CCNGG	1 cut(s)	540
BstSFI	CTRYAG	2 cut(s)	163, 1154
BstV1I	GCAGC	2 cut(s)	73, 1228
BstX2I	RGATCY	2 cut(s)	725, 906
BstXI	CCANNNNNNTGG	1 cut(s)	397
BstYI	RGATCY	2 cut(s)	725, 906
BsuRI	GGCC	3 cut(s)	489, 545, 861
BtsCI	GGATG	1 cut(s)	1216
BtsIMutI	CAGTG	5 cut(s)	363, 474, 818, 881, 1182
Cac8I	GCNNGC	1 cut(s)	1239
CciI	TCATGA	2 cut(s)	196, 850
Cfr13I	GGNCC	1 cut(s)	488
Csp6I	GTAC	3 cut(s)	49, 521, 1195
CviAII	CATG	6 cut(s)	83, 197, 413, 454, 565, 851
CviQI	GTAC	3 cut(s)	49, 521, 1195
DdeI	CTNAG	4 cut(s)	223, 656, 987, 1107
DpnI	GATC	6 cut(s)	268, 362, 727, 908, 1078, 1188
DpnII	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
EaeI	YGGCCR	1 cut(s)	543
Eam1104I	CTCTTC	2 cut(s)	257, 645
EarI	CTCTTC	2 cut(s)	257, 645
Ecl136II	GAGCTC	1 cut(s)	655
Eco130I	CCWWGG	2 cut(s)	251, 1190
Eco24I	GRGCYC	1 cut(s)	657
Eco31I	GGTCTC	1 cut(s)	299
Eco32I	GATATC	1 cut(s)	42
Eco53kI	GAGCTC	1 cut(s)	655
Eco57I	CTGAAG	1 cut(s)	430
EcoICRI	GAGCTC	1 cut(s)	655
EcoRII	CCWGG	1 cut(s)	540
EcoRV	GATATC	1 cut(s)	42
EcoT14I	CCWWGG	2 cut(s)	251, 1190
EcoT38I	GRGCYC	1 cut(s)	657
ErhI	CCWWGG	2 cut(s)	251, 1190
FaeI	CATG	6 cut(s)	86, 200, 416, 457, 568, 854
FatI	CATG	6 cut(s)	82, 196, 412, 453, 564, 850
Fnu4HI	GCNGC	4 cut(s)	77, 87, 181, 1242
FokI	GGATG	1 cut(s)	1203
FriOI	GRGCYC	1 cut(s)	657
Fsp4HI	GCNGC	4 cut(s)	77, 87, 181, 1242
FspBI	CTAG	1 cut(s)	941
GluI	GCNGC	4 cut(s)	77, 87, 181, 1242
HaeIII	GGCC	3 cut(s)	489, 545, 861
Hin1II	CATG	6 cut(s)	86, 200, 416, 457, 568, 854
HindIII	AAGCTT	2 cut(s)	1051, 1112
HinfI	GANTC	3 cut(s)	221, 783, 1005
HphI	GGTGA	3 cut(s)	545, 625, 662
Hpy166II	GTNNAC	1 cut(s)	633
Hpy188I	TCNGA	1 cut(s)	449
Hpy188III	TCNNGA	6 cut(s)	58, 156, 170, 197, 644, 851
Hpy8I	GTNNAC	1 cut(s)	633
HpyAV	CCTTC	2 cut(s)	116, 116
HpyCH4III	ACNGT	4 cut(s)	478, 813, 885, 924
HpyCH4V	TGCA	6 cut(s)	329, 506, 816, 904, 1130, 1202
HpyF10VI	GCNNNNNNNGC	2 cut(s)	845, 1136
HpyF3I	CTNAG	4 cut(s)	223, 656, 987, 1107
Hsp92II	CATG	6 cut(s)	86, 200, 416, 457, 568, 854
Kzo9I	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
Lsp1109I	GCAGC	2 cut(s)	73, 1228
LweI	GCATC	1 cut(s)	1087
MaeI	CTAG	1 cut(s)	941
MaeIII	GTNAC	4 cut(s)	153, 205, 354, 740
MalI	GATC	6 cut(s)	268, 362, 727, 908, 1078, 1188
MboI	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
MboII	GAAGA	5 cut(s)	274, 436, 632, 675, 715
MflI	RGATCY	2 cut(s)	725, 906
MhlI	GDGCHC	1 cut(s)	657
MlsI	TGGCCA	1 cut(s)	545
MluCI	AATT	8 cut(s)	228, 465, 510, 516, 666, 804, 1085, 1165
MluNI	TGGCCA	1 cut(s)	545
MlyI	GAGTC	2 cut(s)	215, 999
MmeI	TCCRAC	1 cut(s)	125
Mox20I	TGGCCA	1 cut(s)	545
MscI	TGGCCA	1 cut(s)	545
MseI	TTAA	7 cut(s)	420, 459, 624, 771, 803, 1062, 1149
MslI	CAYNNNNRTG	2 cut(s)	395, 747
Msp20I	TGGCCA	1 cut(s)	545
MspR9I	CCNGG	1 cut(s)	542
Mva1269I	GAATGC	1 cut(s)	560
MvaI	CCWGG	1 cut(s)	542
MwoI	GCNNNNNNNGC	2 cut(s)	845, 1136
NdeII	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
NlaIII	CATG	6 cut(s)	86, 200, 416, 457, 568, 854
NmeAIII	GCCGAG	1 cut(s)	465
NmuCI	GTSAC	4 cut(s)	153, 205, 354, 740
NspI	RCATGY	1 cut(s)	86
OliI	CACNNNNGTG	1 cut(s)	395
PagI	TCATGA	2 cut(s)	196, 850
PctI	GAATGC	1 cut(s)	560
PfeI	GAWTC	1 cut(s)	783
PkrI	GCNGC	4 cut(s)	78, 88, 182, 1243
PleI	GAGTC	2 cut(s)	215, 999
PpsI	GAGTC	2 cut(s)	215, 999
PshBI	ATTAAT	1 cut(s)	803
Psp124BI	GAGCTC	1 cut(s)	657
Psp6I	CCWGG	1 cut(s)	540
PspGI	CCWGG	1 cut(s)	540
PspPI	GGNCC	1 cut(s)	488
PsuI	RGATCY	2 cut(s)	725, 906
RsaI	GTAC	3 cut(s)	50, 522, 1196
RsaNI	GTAC	3 cut(s)	49, 521, 1195
RseI	CAYNNNNRTG	2 cut(s)	395, 747
SacI	GAGCTC	1 cut(s)	657
SaqAI	TTAA	7 cut(s)	420, 459, 624, 771, 803, 1062, 1149
SatI	GCNGC	4 cut(s)	77, 87, 181, 1242
Sau3AI	GATC	6 cut(s)	266, 360, 725, 906, 1076, 1186
Sau96I	GGNCC	1 cut(s)	488
SchI	GAGTC	2 cut(s)	215, 999
ScrFI	CCNGG	1 cut(s)	542
SduI	GDGCHC	1 cut(s)	657
SfaNI	GCATC	1 cut(s)	1087
SfcI	CTRYAG	2 cut(s)	163, 1154
SmiMI	CAYNNNNRTG	2 cut(s)	395, 747
SmlI	CTYRAG	2 cut(s)	689, 952
SmoI	CTYRAG	2 cut(s)	689, 952
Sse9I	AATT	8 cut(s)	228, 465, 510, 516, 666, 804, 1085, 1165
SsiI	CCGC	2 cut(s)	76, 181
SspMI	CTAG	1 cut(s)	941
SstI	GAGCTC	1 cut(s)	657
StyD4I	CCNGG	1 cut(s)	540
StyI	CCWWGG	2 cut(s)	251, 1190
TaaI	ACNGT	4 cut(s)	478, 813, 885, 924
TaqI	TCGA	4 cut(s)	57, 241, 1135, 1230
TasI	AATT	8 cut(s)	228, 465, 510, 516, 666, 804, 1085, 1165
TatI	WGTACW	2 cut(s)	48, 520
TauI	GCSGC	2 cut(s)	79, 183
TfiI	GAWTC	1 cut(s)	783
Tru1I	TTAA	7 cut(s)	420, 459, 624, 771, 803, 1062, 1149
Tru9I	TTAA	7 cut(s)	420, 459, 624, 771, 803, 1062, 1149
TscAI	CASTG	5 cut(s)	370, 481, 818, 888, 1189
TseFI	GTSAC	4 cut(s)	153, 205, 354, 740
TseI	GCWGC	2 cut(s)	86, 1241
Tsp45I	GTSAC	4 cut(s)	153, 205, 354, 740
TspDTI	ATGAA	5 cut(s)	185, 324, 553, 879, 1193
TspRI	CASTG	5 cut(s)	370, 481, 818, 888, 1189
VspI	ATTAAT	1 cut(s)	803
XapI	RAATTY	3 cut(s)	465, 1085, 1165
XceI	RCATGY	1 cut(s)	86
XspI	CTAG	1 cut(s)	941

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.