Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0001129

Physical Location & Seq

Genomic Coordinates Forward (+)

36483542 .. 36485265

Gene Structure 1724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0255300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGCCCAGCAGTTGCAAAACCTCCCAGATGTCCTGATCATCAAACCACCACCGGCCTTCATATCTTTCGAATCCGACTTCACCCAAAAGTTCAAGATCCTCCGAGCATGGAAATCACCACTCCCTCTGGACCAGTTCCTCACCAAGCACGCCCAATCAGTCCAGGCCCTCCTTTGCCGCGCTGTGACTCCGGTCAACGCCGACCTCCTCCAGCTGCTACCGGCCCTGAAGCTCGTGGTGACTGTCAGCACCGGCGCCAATCACGTGGACAAAGCGGAGTGCCGGCGGCGTGGGATTGCTGTGGCCAATGCCGGAAATGCGTACTCAGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGATGTGAATCGGAGAATTTCAGCAGCTGATTGGTATGTGAGACGTGGGCTTTGGTCTAGCAAAGGAGACTATCCTCTTAGTTCCAAGATAGGTGGAAAGCGTGTAGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTTGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGGAGTGAAAGGCCATCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAATGATGCTCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGATAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGACCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAAGTCTGTTTATACCCTAGAGTCTTTCAAGAGAGGAAGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAAACCGTTGATTACTCCAGCCTATGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005789 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0051604 GO:0070011 GO:0071586 GO:0071704 GO:0080120 GO:0098827 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00900 ko01130 map00900 map01130

Pfam Domains

Protein Families

Peptidase_M48 Peptidase_M48_N

Protein Analysis

323

Amino Acids

35.32

Weight (kDa)

8.59

Isoelectric Point (pI)

40.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	45 - 318	1.3e-21	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	118 - 288	1.7e-49	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001129)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01320 AT4G01320 AT4G01320
fragaria_vesca	FvH4_7g15081 FvH4_7g15081 FvH4_7g15081 FvH4_7g15083
malus_domestica	MD01G1069400.v1.1
prunus_persica	Prupe.2G175500_v2.0.a1 Prupe.2G175500_v2.0.a1 Prupe.2G175500_v2.0.a1
pyrus_communis	pycom01g09880
rosa_chinensis	RchiOBHm_Chr1g0357201 RchiOBHm_Chr1g0357211 RchiOBHm_Chr1g0357271 RchiOBHm_Chr3g0493601
rosa_laevigata	RLG00000018289 RLG00000028032 RLG00000028038 RLG00000028039 RLG00000028042 RLG00000028044
rosa_multiflora	Rmu_sc0004075.1_g000007 Rmu_sc0004792.1_g000002 Rmu_sc0005967.1_g000005 Rmu_sc0006798.1_g000014 Rmu_sc0007965.1_g000003 Rmu_sc0017004.1_g000001
rosa_roxburghii	Rroxscaffold_4G00298940 Rroxscaffold_4G00298980 Rroxscaffold_4G00298990
rosa_rugosa	Rorug01G0213800 Rorug01G0254900 Rorug01G0255300 Rorug01G0255400 Rorug01G0255600 Rorug01G0255800 Rorug01G0255900 Rorug04G0302100 Rorug04G0302200 Rorug04G0302300 Rorug04G0302400
rosa_samantha	Rh1AG269100 Rh1AG269200 Rh1CG150800 Rh1CG252800 Rh1CG252900 Rh1CG253400 Rh1DG264200 Rh1DG264300 Rh3AG311700
rosa_wichuraiana	Rw1G023750 Rw1G023760 Rw1G023770 Rw1G023780 Rw1G023800 Rw1G023840 Rw1G024020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	254
AccB7I	CCANNNNNTGG	1 cut(s)	965
AccII	CGCG	1 cut(s)	180
AciI	CCGC	4 cut(s)	178, 275, 286, 655
AclWI	GGATC	1 cut(s)	91
AcoI	YGGCCR	1 cut(s)	303
AcsI	RAATTY	1 cut(s)	378
AcuI	CTGAAG	1 cut(s)	248
AcvI	CACGTG	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	1 cut(s)	323
AfiI	CCNNNNNNNGG	1 cut(s)	965
AgsI	TTSAA	2 cut(s)	94, 892
AjiI	CACGTC	1 cut(s)	407
AjnI	CCWGG	1 cut(s)	162
AluBI	AGCT	6 cut(s)	214, 232, 389, 601, 608, 929
AluI	AGCT	6 cut(s)	214, 232, 389, 601, 608, 929
Alw26I	GTCTC	3 cut(s)	397, 423, 861
AlwI	GGATC	1 cut(s)	91
AlwNI	CAGNNNCTG	1 cut(s)	389
AoxI	GGCC	7 cut(s)	3, 54, 165, 222, 303, 519, 560
ApeKI	GCWGC	4 cut(s)	214, 386, 528, 605
ApoI	RAATTY	1 cut(s)	378
ArsI	GACNNNNNNTTYG	2 cut(s)	396, 428
AspLEI	GCGC	2 cut(s)	182, 257
AspS9I	GGNCC	4 cut(s)	4, 130, 166, 223
AsuHPI	GGTGA	5 cut(s)	73, 108, 133, 250, 775
AsuII	TTCGAA	1 cut(s)	69
AvaII	GGWCC	1 cut(s)	130
BalI	TGGCCA	1 cut(s)	305
BanI	GGYRCC	1 cut(s)	254
BauI	CACGAG	1 cut(s)	233
BbrPI	CACGTG	1 cut(s)	265
BbvI	GCAGC	4 cut(s)	201, 398, 515, 617
BccI	CCATC	2 cut(s)	571, 692
BciT130I	CCWGG	1 cut(s)	164
BclI	TGATCA	2 cut(s)	36, 663
BcoDI	GTCTC	3 cut(s)	397, 423, 861
BfaI	CTAG	2 cut(s)	420, 881
BfoI	RGCGCY	1 cut(s)	258
BglII	AGATCT	1 cut(s)	534
BisI	GCNGC	6 cut(s)	178, 215, 287, 387, 529, 606
BlsI	GCNGC	6 cut(s)	179, 216, 288, 388, 530, 607
Bme1390I	CCNGG	1 cut(s)	164
Bme18I	GGWCC	1 cut(s)	130
BmgBI	CACGTC	1 cut(s)	407
BmgT120I	GGNCC	4 cut(s)	4, 130, 166, 223
BmiI	GGNNCC	1 cut(s)	256
BmrFI	CCNGG	1 cut(s)	164
BmsI	GCATC	1 cut(s)	607
BoxI	GACNNNNGTC	1 cut(s)	191
BpmI	CTGGAG	2 cut(s)	194, 942
Bpu14I	TTCGAA	1 cut(s)	69
BpuEI	CTTGAG	2 cut(s)	532, 536
Bsa29I	ATCGAT	1 cut(s)	363
BsaAI	YACGTR	1 cut(s)	265
BsaBI	GATNNNNATC	1 cut(s)	369
BsaHI	GRCGYC	1 cut(s)	255
BsaWI	WCCGGW	1 cut(s)	190
BsaXI	ACNNNNNCTCC	2 cut(s)	693, 723
Bsc4I	CCNNNNNNNGG	1 cut(s)	965
Bse118I	RCCGGY	4 cut(s)	52, 220, 251, 282
Bse1I	ACTGG	1 cut(s)	133
Bse3DI	GCAATG	1 cut(s)	843
Bse8I	GATNNNNATC	1 cut(s)	369
BseBI	CCWGG	1 cut(s)	164
BseCI	ATCGAT	1 cut(s)	363
BseGI	GGATG	1 cut(s)	799
BseJI	GATNNNNATC	1 cut(s)	369
BseLI	CCNNNNNNNGG	1 cut(s)	965
BseMI	GCAATG	1 cut(s)	843
BseMII	CTCAG	1 cut(s)	339
BseNI	ACTGG	1 cut(s)	133
BseRI	GAGGAG	1 cut(s)	197
BseXI	GCAGC	4 cut(s)	201, 398, 515, 617
BseYI	CCCAGC	1 cut(s)	6
Bsh1236I	CGCG	1 cut(s)	180
BshFI	GGCC	7 cut(s)	5, 56, 167, 224, 305, 521, 562
BshNI	GGYRCC	1 cut(s)	254
BshVI	ATCGAT	1 cut(s)	363
BsiSI	CCGG	6 cut(s)	53, 191, 221, 252, 283, 312
BslI	CCNNNNNNNGG	1 cut(s)	965
BsmAI	GTCTC	3 cut(s)	397, 423, 861
BsmBI	CGTCTC	1 cut(s)	397
BsnI	GGCC	7 cut(s)	5, 56, 167, 224, 305, 521, 562
Bsp119I	TTCGAA	1 cut(s)	69
Bsp143I	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
BspACI	CCGC	4 cut(s)	178, 275, 286, 655
BspANI	GGCC	7 cut(s)	5, 56, 167, 224, 305, 521, 562
BspCNI	CTCAG	1 cut(s)	338
BspDI	ATCGAT	1 cut(s)	363
BspFNI	CGCG	1 cut(s)	180
BspLI	GGNNCC	1 cut(s)	256
BspPI	GGATC	1 cut(s)	91
BspT104I	TTCGAA	1 cut(s)	69
BspT107I	GGYRCC	1 cut(s)	254
BsrDI	GCAATG	1 cut(s)	843
BsrFI	RCCGGY	4 cut(s)	52, 220, 251, 282
BsrI	ACTGG	1 cut(s)	133
BssAI	RCCGGY	4 cut(s)	52, 220, 251, 282
BssMI	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	1 cut(s)	233
Bst2BI	CACGAG	1 cut(s)	233
Bst2UI	CCWGG	1 cut(s)	164
Bst4CI	ACNGT	2 cut(s)	244, 948
BstACI	GRCGYC	1 cut(s)	255
BstBAI	YACGTR	1 cut(s)	265
BstBI	TTCGAA	1 cut(s)	69
BstC8I	GCNNGC	3 cut(s)	150, 284, 603
BstDEI	CTNAG	2 cut(s)	325, 440
BstF5I	GGATG	1 cut(s)	799
BstFNI	CGCG	1 cut(s)	180
BstH2I	RGCGCY	1 cut(s)	258
BstHHI	GCGC	2 cut(s)	182, 257
BstKTI	GATC	6 cut(s)	39, 99, 537, 666, 777, 915
BstMAI	GTCTC	3 cut(s)	397, 423, 861
BstMBI	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
BstMWI	GCNNNNNNNGC	2 cut(s)	317, 344
BstNI	CCWGG	1 cut(s)	164
BstPAI	GACNNNNGTC	1 cut(s)	191
BstSCI	CCNGG	1 cut(s)	162
BstUI	CGCG	1 cut(s)	180
BstV1I	GCAGC	4 cut(s)	201, 398, 515, 617
BstX2I	RGATCY	2 cut(s)	96, 534
BstXI	CCANNNNNNTGG	1 cut(s)	265
BstYI	RGATCY	2 cut(s)	96, 534
Bsu15I	ATCGAT	1 cut(s)	363
BsuRI	GGCC	7 cut(s)	5, 56, 167, 224, 305, 521, 562
BsuTUI	ATCGAT	1 cut(s)	363
BtrI	CACGTC	1 cut(s)	407
BtsCI	GGATG	1 cut(s)	799
Cac8I	GCNNGC	3 cut(s)	150, 284, 603
CaiI	CAGNNNCTG	1 cut(s)	389
CfoI	GCGC	2 cut(s)	182, 257
Cfr10I	RCCGGY	4 cut(s)	52, 220, 251, 282
Cfr13I	GGNCC	4 cut(s)	4, 130, 166, 223
ClaI	ATCGAT	1 cut(s)	363
Csp6I	GTAC	1 cut(s)	322
CspCI	CAANNNNNGTGG	2 cut(s)	436, 471
CviAII	CATG	2 cut(s)	108, 661
CviQI	GTAC	1 cut(s)	322
DdeI	CTNAG	2 cut(s)	325, 440
DinI	GGCGCC	1 cut(s)	256
DpnI	GATC	6 cut(s)	38, 98, 536, 665, 776, 914
DpnII	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
EaeI	YGGCCR	1 cut(s)	303
Eco147I	AGGCCT	1 cut(s)	521
Eco32I	GATATC	1 cut(s)	343
Eco47I	GGWCC	1 cut(s)	130
Eco57I	CTGAAG	1 cut(s)	248
Eco72I	CACGTG	1 cut(s)	265
EcoO109I	RGGNCCY	1 cut(s)	166
EcoRII	CCWGG	1 cut(s)	162
EcoRV	GATATC	1 cut(s)	343
EgeI	GGCGCC	1 cut(s)	256
EheI	GGCGCC	1 cut(s)	256
Esp3I	CGTCTC	1 cut(s)	397
FaeI	CATG	2 cut(s)	111, 664
FalI	AAGNNNNNCTT	4 cut(s)	550, 582, 662, 694
FatI	CATG	2 cut(s)	107, 660
FauI	CCCGC	1 cut(s)	662
FbaI	TGATCA	2 cut(s)	36, 663
Fnu4HI	GCNGC	6 cut(s)	178, 215, 287, 387, 529, 606
FokI	GGATG	1 cut(s)	806
Fsp4HI	GCNGC	6 cut(s)	178, 215, 287, 387, 529, 606
FspBI	CTAG	2 cut(s)	420, 881
GlaI	GCGC	2 cut(s)	181, 256
GluI	GCNGC	6 cut(s)	178, 215, 287, 387, 529, 606
GsaI	CCCAGC	1 cut(s)	10
GsuI	CTGGAG	2 cut(s)	194, 942
HaeII	RGCGCY	1 cut(s)	258
HaeIII	GGCC	7 cut(s)	5, 56, 167, 224, 305, 521, 562
HapII	CCGG	6 cut(s)	53, 191, 221, 252, 283, 312
HhaI	GCGC	2 cut(s)	182, 257
Hin1I	GRCGYC	1 cut(s)	255
Hin1II	CATG	2 cut(s)	111, 664
Hin6I	GCGC	2 cut(s)	180, 255
HinP1I	GCGC	2 cut(s)	180, 255
HincII	GTYRAC	1 cut(s)	196
HindII	GTYRAC	1 cut(s)	196
HindIII	AAGCTT	1 cut(s)	927
HinfI	GANTC	4 cut(s)	71, 187, 370, 884
HpaII	CCGG	6 cut(s)	53, 191, 221, 252, 283, 312
HphI	GGTGA	5 cut(s)	73, 108, 133, 250, 775
Hpy166II	GTNNAC	2 cut(s)	196, 268
Hpy188I	TCNGA	5 cut(s)	76, 104, 328, 375, 779
Hpy188III	TCNNGA	4 cut(s)	34, 94, 128, 892
Hpy8I	GTNNAC	2 cut(s)	196, 268
HpyAV	CCTTC	2 cut(s)	67, 589
HpyCH4III	ACNGT	2 cut(s)	244, 948
HpyCH4IV	ACGT	2 cut(s)	264, 406
HpyCH4V	TGCA	5 cut(s)	16, 531, 605, 638, 686
HpyF10VI	GCNNNNNNNGC	2 cut(s)	317, 344
HpyF3I	CTNAG	2 cut(s)	325, 440
HpySE526I	ACGT	2 cut(s)	264, 406
Hsp92I	GRCGYC	1 cut(s)	255
Hsp92II	CATG	2 cut(s)	111, 664
HspAI	GCGC	2 cut(s)	180, 255
KasI	GGCGCC	1 cut(s)	254
KroI	GCCGGC	1 cut(s)	282
KroNI	GCCGGC	1 cut(s)	284
Ksp22I	TGATCA	2 cut(s)	36, 663
Kzo9I	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
Lsp1109I	GCAGC	4 cut(s)	201, 398, 515, 617
LweI	GCATC	1 cut(s)	607
MaeI	CTAG	2 cut(s)	420, 881
MaeII	ACGT	2 cut(s)	264, 406
MaeIII	GTNAC	2 cut(s)	184, 238
MalI	GATC	6 cut(s)	38, 98, 536, 665, 776, 914
MboI	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
MboII	GAAGA	2 cut(s)	341, 925
MflI	RGATCY	2 cut(s)	96, 534
MlsI	TGGCCA	1 cut(s)	305
MluCI	AATT	4 cut(s)	378, 729, 746, 919
MluNI	TGGCCA	1 cut(s)	305
Mly113I	GGCGCC	1 cut(s)	255
MlyI	GAGTC	2 cut(s)	181, 893
MmeI	TCCRAC	2 cut(s)	99, 457
Mox20I	TGGCCA	1 cut(s)	305
MroNI	GCCGGC	1 cut(s)	282
MscI	TGGCCA	1 cut(s)	305
MseI	TTAA	1 cut(s)	711
MslI	CAYNNNNRTG	1 cut(s)	365
Msp20I	TGGCCA	1 cut(s)	305
MspA1I	CMGCKG	2 cut(s)	214, 389
MspI	CCGG	6 cut(s)	53, 191, 221, 252, 283, 312
MspR9I	CCNGG	1 cut(s)	164
MvaI	CCWGG	1 cut(s)	164
MvnI	CGCG	1 cut(s)	180
MwoI	GCNNNNNNNGC	2 cut(s)	317, 344
NaeI	GCCGGC	1 cut(s)	284
NarI	GGCGCC	1 cut(s)	255
NdeII	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
NgoMIV	GCCGGC	1 cut(s)	282
NlaIII	CATG	2 cut(s)	111, 664
NlaIV	GGNNCC	1 cut(s)	256
NmuCI	GTSAC	2 cut(s)	184, 238
NspV	TTCGAA	1 cut(s)	69
PceI	AGGCCT	1 cut(s)	521
PdiI	GCCGGC	1 cut(s)	284
PfeI	GAWTC	2 cut(s)	71, 370
PflMI	CCANNNNNTGG	1 cut(s)	965
PkrI	GCNGC	6 cut(s)	179, 216, 288, 388, 530, 607
PleI	GAGTC	2 cut(s)	181, 892
PluTI	GGCGCC	1 cut(s)	258
PmaCI	CACGTG	1 cut(s)	265
PmlI	CACGTG	1 cut(s)	265
PpsI	GAGTC	2 cut(s)	181, 892
Ppu21I	YACGTR	1 cut(s)	265
PshAI	GACNNNNGTC	1 cut(s)	191
Psp6I	CCWGG	1 cut(s)	162
PspCI	CACGTG	1 cut(s)	265
PspFI	CCCAGC	1 cut(s)	6
PspGI	CCWGG	1 cut(s)	162
PspN4I	GGNNCC	1 cut(s)	256
PspPI	GGNCC	4 cut(s)	4, 130, 166, 223
PstNI	CAGNNNCTG	1 cut(s)	389
PsuI	RGATCY	2 cut(s)	96, 534
PvuII	CAGCTG	2 cut(s)	214, 389
RsaI	GTAC	1 cut(s)	323
RsaNI	GTAC	1 cut(s)	322
RseI	CAYNNNNRTG	1 cut(s)	365
SaqAI	TTAA	1 cut(s)	711
SatI	GCNGC	6 cut(s)	178, 215, 287, 387, 529, 606
Sau3AI	GATC	6 cut(s)	36, 96, 534, 663, 774, 912
Sau96I	GGNCC	4 cut(s)	4, 130, 166, 223
SchI	GAGTC	2 cut(s)	181, 893
ScrFI	CCNGG	1 cut(s)	164
SfaNI	GCATC	1 cut(s)	607
SfoI	GGCGCC	1 cut(s)	256
SfuI	TTCGAA	1 cut(s)	69
SgrAI	CRCCGGYG	1 cut(s)	251
SinI	GGWCC	1 cut(s)	130
SmiMI	CAYNNNNRTG	1 cut(s)	365
SmlI	CTYRAG	2 cut(s)	515, 547
SmoI	CTYRAG	2 cut(s)	515, 547
Sse9I	AATT	4 cut(s)	378, 729, 746, 919
SseBI	AGGCCT	1 cut(s)	521
SsiI	CCGC	4 cut(s)	178, 275, 286, 655
SspDI	GGCGCC	1 cut(s)	254
SspMI	CTAG	2 cut(s)	420, 881
StuI	AGGCCT	1 cut(s)	521
StyD4I	CCNGG	1 cut(s)	162
TaaI	ACNGT	2 cut(s)	244, 948
TaiI	ACGT	2 cut(s)	267, 409
TaqI	TCGA	2 cut(s)	69, 363
TasI	AATT	4 cut(s)	378, 729, 746, 919
TauI	GCSGC	2 cut(s)	180, 289
TfiI	GAWTC	2 cut(s)	71, 370
Tru1I	TTAA	1 cut(s)	711
Tru9I	TTAA	1 cut(s)	711
TseFI	GTSAC	2 cut(s)	184, 238
TseI	GCWGC	4 cut(s)	214, 386, 528, 605
Tsp45I	GTSAC	2 cut(s)	184, 238
TspDTI	ATGAA	3 cut(s)	49, 349, 513
Van91I	CCANNNNNTGG	1 cut(s)	965
VpaK11BI	GGWCC	1 cut(s)	130
XapI	RAATTY	1 cut(s)	378
XspI	CTAG	2 cut(s)	420, 881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.