Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000774

Physical Location & Seq

Genomic Coordinates Reverse (-)

35591039 .. 35594915

Gene Structure 3877 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1272500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGGCATTAAGGAGGATGTGGAGAGTGATGTGCAGGAGGGAGTAAGAGTGCCACTCATGGCCGAAGGAGACGAATCAGCTGGTGGAAGCAGCGGAGGGAATCTATGGATGGTTTATCTCAGCACATTTGTTGCAGTTTGTGGCTCTTATGAGTTCGGATGCTGTGTCGGTTATTCTTCGCCTACTCAGTCTGCAATCCGGGAAGATCTCAGTTTATCATTATCAGAGTATTCAGTGTTTGGCTCCATTTTGACATTTGGTGCAATGATAGGAGCTATCACTATTGGGCCAATCACCGATTTTCTTGGCCGAAAAGGGGCCTTGAGAGTGTCTTGTGCTTTCTGTGTTGCAGGTTGGCTTGCTATTTACTTCTCCAAGGGCGCTTGGTCGTTGGACATTGGGAGACTGCTAACAGGGTACGGAATGGGAGCCTTTTCTTATGTGGTACCTATTTTCATAGCTGAAATTGCACCCAAAAGCCTTCGAGGAAGATTGACTGCTGCAAATCAGTTAATGATCGTAACTGGAGTGTCTGTTTCCTTCATAATCGGGGTAGTAGTAAGTTGGCGGACATTAGCATTAATTGGACTTATTCCCTGCGCCGTGACACTTTTTGGTCTCTTTTTCATTCCCGAGTCTCCAAGATGGTTGGCAAAGACAGGACGCCAGGAAGATTTTGAAGCTGCGCTGCAGAAACTGCGTGGAAAAGATGCTGACATATCTCATGAAGCAGCAGAAATCCAGGATTATATTGTGACTCTTGATTCCCTCCCTAAAGCCAAATTGCTGGATTTGTTTCAAAGGAGATACTGGCGCTCACTCATTATCGGAATTGGGCTGATGGTCTGTCAACAACTGGGTGGAATCAACGGAGTCTGTTTCTATGTCAGCGATATTTTCGAACAAGCAGGATTTTCCTCCAGCATTGGAACTATAATCTATGCTATTCTTCAGGTTATAGTTACCGGCATTGCTGCAGGCGTGATGGATAAAGCTGGACGAAAGCCTCTAATTTTGGTCTCTGCATCCGGGTTGGTGCTTGGCTCTCTCATGACAGCGGTTTCATTCTTTCTGAAGGTTCATGAATTAGCACTCGACGCAGTTCCCATACTTGCTGTAGCCGGCATACTGGTATACATAGTAGCCTTTTCGATAGGAATGGGAGCAGTTCCTTGGGTTGTGATGTCTGAGATATTCCCGATAAATATTAAAGGGCAGGCCGGAAGCTTAGCAACGCTAGTGAACTGGTTCGGCGCATGGTTGTGTTCCTACACGTTCAACTATCTTATGAGTTGGAGCTCCTACGGTACATTCATTCTGTACGCCGGAATCAACACTCTTGCTATTTTGTTCGTGGTGTCGTTGGTGCCCGAAACAAAAGGGAAAACACTGGAACAGATCCAAGGAGCCATGAACAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0000325 GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009507 GO:0009536 GO:0009705 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0034219 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0071702 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

474

Amino Acids

50.71

Weight (kDa)

5.61

Isoelectric Point (pI)

32.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	53 - 468	1.4e-93	Sugar (and other) transporter
MFS_1	PF07690	58 - 418	5e-27	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48020 AT2G48020 AT2G48020 AT2G48020
fragaria_vesca	FvH4_2g24260 FvH4_2g24260 FvH4_2g24280 FvH4_2g24280 FvH4_2g24280 FvH4_2g24290
malus_domestica	MD03G1272500.v1.1 MD04G1211000.v1.1 MD11G1293100.v1.1 MD11G1293200.v1.1 MD11G1293300.v1.1 MD11G1293400.v1.1 MD12G1225800.v1.1
prunus_persica	Prupe.8G248500_v2.0.a1 Prupe.8G248600_v2.0.a1 Prupe.8G248600_v2.0.a1 Prupe.8G248600_v2.0.a1 Prupe.8G248600_v2.0.a1 Prupe.8G248600_v2.0.a1 Prupe.8G248600_v2.0.a1 Prupe.8G248700_v2.0.a1
pyrus_communis	pycom03g21700 pycom11g25940 pycom11g25950 pycom11g25960
rosa_chinensis	RchiOBHm_Chr6g0292071 RchiOBHm_Chr6g0292101 RchiOBHm_Chr6g0292121 RchiOBHm_Chr6g0292141
rosa_laevigata	RLG00000012058 RLG00000012060 RLG00000012061
rosa_multiflora	Rmu_co8240599.1_g000001 Rmu_co8452453.1_g000001 Rmu_sc0000303.1_g000031 Rmu_sc0000502.1_g000008 Rmu_sc0000502.1_g000011
rosa_roxburghii	Rroxscaffold_7G00175410 Rroxscaffold_7G00175430 Rroxscaffold_7G00175460
rosa_rugosa	Rorug06G0223300 Rorug06G0223400 Rorug06G0223400 Rorug06G0223500 Rorug06G0223600 Rorug06G0223700 Rorug06G0223800
rosa_samantha	Rh2AG603100 Rh3BG352500 Rh6AG336800 Rh6AG336900 Rh6AG337100 Rh6BG344100 Rh6BG344200 Rh6BG344300 Rh6CG350500 Rh6CG350800 Rh6CG351100 Rh6DG337000 Rh6DG337300 Rh7BG160000 Rh7BG460400
rosa_wichuraiana	Rw6G029310 Rw6G029320 Rw6G029330 Rw6G029340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	341
Acc65I	GGTACC	1 cut(s)	445
AccB1I	GGYRCC	2 cut(s)	445, 1366
AccI	GTMKAC	1 cut(s)	1134
AciI	CCGC	3 cut(s)	93, 568, 1058
AclWI	GGATC	1 cut(s)	1393
AcoI	YGGCCR	2 cut(s)	60, 307
AcuI	CTGAAG	2 cut(s)	935, 1094
AcyI	GRCGYC	1 cut(s)	664
AfaI	GTAC	4 cut(s)	419, 447, 1309, 1322
AfiI	CCNNNNNNNGG	1 cut(s)	316
AflIII	ACRYGT	1 cut(s)	1272
AgsI	TTSAA	3 cut(s)	680, 800, 1279
AjnI	CCWGG	2 cut(s)	666, 741
AluBI	AGCT	7 cut(s)	80, 275, 461, 683, 995, 1227, 1299
AluI	AGCT	7 cut(s)	80, 275, 461, 683, 995, 1227, 1299
Alw21I	GWGCWC	1 cut(s)	1301
Alw26I	GTCTC	5 cut(s)	63, 397, 623, 642, 1024
AlwI	GGATC	1 cut(s)	1393
AlwNI	CAGNNNCTG	1 cut(s)	697
Ama87I	CYCGRG	1 cut(s)	632
AoxI	GGCC	5 cut(s)	60, 287, 307, 318, 1218
ApeKI	GCWGC	6 cut(s)	90, 500, 683, 688, 731, 974
AseI	ATTAAT	1 cut(s)	581
Asp700I	GAANNNNTTC	1 cut(s)	1247
Asp718I	GGTACC	1 cut(s)	445
AspLEI	GCGC	5 cut(s)	383, 602, 688, 816, 1256
AspS9I	GGNCC	2 cut(s)	287, 318
AsuC2I	CCSGG	2 cut(s)	200, 1030
AsuHPI	GGTGA	1 cut(s)	286
AsuII	TTCGAA	1 cut(s)	900
AvaI	CYCGRG	1 cut(s)	632
BaeGI	GKGCMC	1 cut(s)	1371
BanI	GGYRCC	2 cut(s)	445, 1366
BanII	GRGCYC	1 cut(s)	1301
Bbv12I	GWGCWC	1 cut(s)	1301
BbvI	GCAGC	6 cut(s)	102, 487, 670, 675, 743, 961
BccI	CCATC	4 cut(s)	103, 639, 835, 979
BceAI	ACGGC	1 cut(s)	587
BciT130I	CCWGG	2 cut(s)	668, 743
BcnI	CCSGG	2 cut(s)	200, 1030
BcoDI	GTCTC	5 cut(s)	63, 397, 623, 642, 1024
BfaI	CTAG	1 cut(s)	1238
BfmI	CTRYAG	3 cut(s)	689, 975, 1116
BfoI	RGCGCY	2 cut(s)	384, 817
BfuAI	ACCTGC	1 cut(s)	341
BglII	AGATCT	1 cut(s)	205
BisI	GCNGC	6 cut(s)	91, 501, 684, 689, 732, 975
BlpI	GCTNAGC	1 cut(s)	1228
BlsI	GCNGC	6 cut(s)	92, 502, 685, 690, 733, 976
Bme1390I	CCNGG	4 cut(s)	200, 668, 743, 1030
BmeT110I	CYCGRG	1 cut(s)	632
BmgT120I	GGNCC	2 cut(s)	287, 318
BmiI	GGNNCC	6 cut(s)	244, 319, 430, 447, 1368, 1408
BmrFI	CCNGG	4 cut(s)	200, 668, 743, 1030
BmrI	ACTGGG	1 cut(s)	866
BmsI	GCATC	3 cut(s)	149, 700, 1034
BmuI	ACTGGG	1 cut(s)	866
BplI	GAGNNNNNCTC	2 cut(s)	39, 71
BpmI	CTGGAG	2 cut(s)	546, 904
Bpu1102I	GCTNAGC	1 cut(s)	1228
Bpu14I	TTCGAA	1 cut(s)	900
BpuEI	CTTGAG	1 cut(s)	343
BpuMI	CCSGG	2 cut(s)	200, 1030
BsaHI	GRCGYC	1 cut(s)	664
BsaI	GGTCTC	2 cut(s)	623, 1024
BsaJI	CCNNGG	3 cut(s)	375, 1172, 1402
BsaXI	ACNNNNNCTCC	4 cut(s)	33, 63, 519, 549
Bsc4I	CCNNNNNNNGG	1 cut(s)	316
Bse118I	RCCGGY	2 cut(s)	965, 1121
Bse1I	ACTGG	6 cut(s)	529, 815, 861, 1134, 1250, 1395
Bse3DI	GCAATG	2 cut(s)	270, 969
BseBI	CCWGG	2 cut(s)	668, 743
BseDI	CCNNGG	3 cut(s)	375, 1172, 1402
BseGI	GGATG	4 cut(s)	22, 114, 164, 1025
BseLI	CCNNNNNNNGG	1 cut(s)	316
BseMI	GCAATG	2 cut(s)	270, 969
BseMII	CTCAG	4 cut(s)	133, 200, 223, 1179
BseNI	ACTGG	6 cut(s)	529, 815, 861, 1134, 1250, 1395
BseSI	GKGCMC	1 cut(s)	1371
BseXI	GCAGC	6 cut(s)	102, 487, 670, 675, 743, 961
BsgI	GTGCAG	1 cut(s)	53
BshFI	GGCC	5 cut(s)	62, 289, 309, 320, 1220
BshNI	GGYRCC	2 cut(s)	445, 1366
BsiHKAI	GWGCWC	1 cut(s)	1301
BsiHKCI	CYCGRG	1 cut(s)	632
BsiSI	CCGG	6 cut(s)	199, 966, 1029, 1122, 1221, 1326
BslI	CCNNNNNNNGG	1 cut(s)	316
BsmAI	GTCTC	5 cut(s)	63, 397, 623, 642, 1024
BsmBI	CGTCTC	1 cut(s)	63
BsnI	GGCC	5 cut(s)	62, 289, 309, 320, 1220
Bso31I	GGTCTC	2 cut(s)	623, 1024
BsoBI	CYCGRG	1 cut(s)	632
Bsp119I	TTCGAA	1 cut(s)	900
Bsp1286I	GDGCHC	2 cut(s)	1301, 1371
Bsp143I	GATC	3 cut(s)	205, 516, 1398
Bsp1720I	GCTNAGC	1 cut(s)	1228
BspACI	CCGC	3 cut(s)	93, 568, 1058
BspANI	GGCC	5 cut(s)	62, 289, 309, 320, 1220
BspCNI	CTCAG	4 cut(s)	132, 199, 222, 1180
BspHI	TCATGA	3 cut(s)	724, 1050, 1081
BspLI	GGNNCC	6 cut(s)	244, 319, 430, 447, 1368, 1408
BspMAI	CTGCAG	2 cut(s)	693, 979
BspMI	ACCTGC	1 cut(s)	341
BspPI	GGATC	1 cut(s)	1393
BspT104I	TTCGAA	1 cut(s)	900
BspT107I	GGYRCC	2 cut(s)	445, 1366
BspTNI	GGTCTC	2 cut(s)	623, 1024
BsrDI	GCAATG	2 cut(s)	270, 969
BsrFI	RCCGGY	2 cut(s)	965, 1121
BsrI	ACTGG	6 cut(s)	529, 815, 861, 1134, 1250, 1395
BssAI	RCCGGY	2 cut(s)	965, 1121
BssECI	CCNNGG	3 cut(s)	375, 1172, 1402
BssMI	GATC	3 cut(s)	205, 516, 1398
BssNAI	GTATAC	1 cut(s)	1135
BssNI	GRCGYC	1 cut(s)	664
BssT1I	CCWWGG	3 cut(s)	375, 1172, 1402
Bst1107I	GTATAC	1 cut(s)	1135
Bst2UI	CCWGG	2 cut(s)	668, 743
Bst4CI	ACNGT	1 cut(s)	1307
BstACI	GRCGYC	1 cut(s)	664
BstAPI	GCANNNNNTGC	1 cut(s)	697
BstBI	TTCGAA	1 cut(s)	900
BstC8I	GCNNGC	4 cut(s)	360, 979, 1123, 1218
BstDEI	CTNAG	5 cut(s)	119, 186, 209, 1188, 1228
BstF5I	GGATG	4 cut(s)	22, 114, 164, 1025
BstH2I	RGCGCY	2 cut(s)	384, 817
BstHHI	GCGC	5 cut(s)	383, 602, 688, 816, 1256
BstKTI	GATC	3 cut(s)	208, 519, 1401
BstMAI	GTCTC	5 cut(s)	63, 397, 623, 642, 1024
BstMBI	GATC	3 cut(s)	205, 516, 1398
BstMWI	GCNNNNNNNGC	3 cut(s)	467, 697, 1097
BstNI	CCWGG	2 cut(s)	668, 743
BstSCI	CCNGG	4 cut(s)	198, 666, 741, 1028
BstSFI	CTRYAG	3 cut(s)	689, 975, 1116
BstSLI	GKGCMC	1 cut(s)	1371
BstV1I	GCAGC	6 cut(s)	102, 487, 670, 675, 743, 961
BstX2I	RGATCY	2 cut(s)	205, 1398
BstXI	CCANNNNNNTGG	1 cut(s)	787
BstYI	RGATCY	2 cut(s)	205, 1398
BstZ17I	GTATAC	1 cut(s)	1135
BsuRI	GGCC	5 cut(s)	62, 289, 309, 320, 1220
BtsCI	GGATG	4 cut(s)	22, 114, 164, 1025
BtsIMutI	CAGTG	2 cut(s)	240, 1388
BveI	ACCTGC	1 cut(s)	341
Cac8I	GCNNGC	4 cut(s)	360, 979, 1123, 1218
CaiI	CAGNNNCTG	1 cut(s)	697
CciI	TCATGA	3 cut(s)	724, 1050, 1081
CfoI	GCGC	5 cut(s)	383, 602, 688, 816, 1256
Cfr10I	RCCGGY	2 cut(s)	965, 1121
Cfr13I	GGNCC	2 cut(s)	287, 318
CseI	GACGC	2 cut(s)	672, 1106
Csp6I	GTAC	4 cut(s)	418, 446, 1308, 1321
CviAII	CATG	6 cut(s)	58, 725, 1051, 1082, 1257, 1411
CviQI	GTAC	4 cut(s)	418, 446, 1308, 1321
DdeI	CTNAG	5 cut(s)	119, 186, 209, 1188, 1228
DpnI	GATC	3 cut(s)	207, 518, 1400
DpnII	GATC	3 cut(s)	205, 516, 1398
EaeI	YGGCCR	2 cut(s)	60, 307
EciI	GGCGGA	1 cut(s)	583
Ecl136II	GAGCTC	1 cut(s)	1299
Eco130I	CCWWGG	3 cut(s)	375, 1172, 1402
Eco24I	GRGCYC	1 cut(s)	1301
Eco31I	GGTCTC	2 cut(s)	623, 1024
Eco53kI	GAGCTC	1 cut(s)	1299
Eco57I	CTGAAG	2 cut(s)	935, 1094
Eco88I	CYCGRG	1 cut(s)	632
EcoICRI	GAGCTC	1 cut(s)	1299
EcoO109I	RGGNCCY	1 cut(s)	318
EcoRII	CCWGG	2 cut(s)	666, 741
EcoT14I	CCWWGG	3 cut(s)	375, 1172, 1402
EcoT38I	GRGCYC	1 cut(s)	1301
ErhI	CCWWGG	3 cut(s)	375, 1172, 1402
Esp3I	CGTCTC	1 cut(s)	63
FaeI	CATG	6 cut(s)	61, 728, 1054, 1085, 1260, 1414
FatI	CATG	6 cut(s)	57, 724, 1050, 1081, 1256, 1410
FblI	GTMKAC	1 cut(s)	1134
Fnu4HI	GCNGC	6 cut(s)	91, 501, 684, 689, 732, 975
FokI	GGATG	4 cut(s)	29, 121, 171, 1012
FriOI	GRGCYC	1 cut(s)	1301
Fsp4HI	GCNGC	6 cut(s)	91, 501, 684, 689, 732, 975
FspBI	CTAG	1 cut(s)	1238
GlaI	GCGC	5 cut(s)	382, 601, 687, 815, 1255
GluI	GCNGC	6 cut(s)	91, 501, 684, 689, 732, 975
GsuI	CTGGAG	2 cut(s)	546, 904
HaeII	RGCGCY	2 cut(s)	384, 817
HaeIII	GGCC	5 cut(s)	62, 289, 309, 320, 1220
HapII	CCGG	6 cut(s)	199, 966, 1029, 1122, 1221, 1326
HgaI	GACGC	2 cut(s)	672, 1106
HhaI	GCGC	5 cut(s)	383, 602, 688, 816, 1256
Hin1I	GRCGYC	1 cut(s)	664
Hin1II	CATG	6 cut(s)	61, 728, 1054, 1085, 1260, 1414
Hin6I	GCGC	5 cut(s)	381, 600, 686, 814, 1254
HinP1I	GCGC	5 cut(s)	381, 600, 686, 814, 1254
HincII	GTYRAC	1 cut(s)	851
HindII	GTYRAC	1 cut(s)	851
HindIII	AAGCTT	1 cut(s)	1225
HinfI	GANTC	8 cut(s)	74, 100, 635, 757, 764, 864, 873, 1329
HpaII	CCGG	6 cut(s)	199, 966, 1029, 1122, 1221, 1326
HphI	GGTGA	1 cut(s)	286
Hpy166II	GTNNAC	3 cut(s)	851, 1135, 1243
Hpy188I	TCNGA	5 cut(s)	158, 226, 830, 1074, 1189
Hpy188III	TCNNGA	6 cut(s)	632, 725, 761, 1051, 1082, 1198
Hpy8I	GTNNAC	3 cut(s)	851, 1135, 1243
Hpy99I	CGWCG	1 cut(s)	1100
HpyAV	CCTTC	4 cut(s)	59, 491, 550, 1069
HpyCH4III	ACNGT	1 cut(s)	1307
HpyCH4IV	ACGT	1 cut(s)	1274
HpyF10VI	GCNNNNNNNGC	3 cut(s)	467, 697, 1097
HpyF3I	CTNAG	5 cut(s)	119, 186, 209, 1188, 1228
HpySE526I	ACGT	1 cut(s)	1274
Hsp92I	GRCGYC	1 cut(s)	664
Hsp92II	CATG	6 cut(s)	61, 728, 1054, 1085, 1260, 1414
HspAI	GCGC	5 cut(s)	381, 600, 686, 814, 1254
KpnI	GGTACC	1 cut(s)	449
KroI	GCCGGC	1 cut(s)	1121
KroNI	GCCGGC	1 cut(s)	1123
Kzo9I	GATC	3 cut(s)	205, 516, 1398
LmnI	GCTCC	7 cut(s)	248, 272, 428, 1163, 1296, 1304, 1406
Lsp1109I	GCAGC	6 cut(s)	102, 487, 670, 675, 743, 961
LweI	GCATC	3 cut(s)	149, 700, 1034
MaeI	CTAG	1 cut(s)	1238
MaeII	ACGT	1 cut(s)	1274
MaeIII	GTNAC	4 cut(s)	520, 604, 754, 961
MalI	GATC	3 cut(s)	207, 518, 1400
MboI	GATC	3 cut(s)	205, 516, 1398
MboII	GAAGA	5 cut(s)	168, 215, 501, 683, 941
MflI	RGATCY	2 cut(s)	205, 1398
MhlI	GDGCHC	2 cut(s)	1301, 1371
MluCI	AATT	6 cut(s)	465, 582, 782, 831, 1011, 1085
MlyI	GAGTC	3 cut(s)	644, 751, 882
MmeI	TCCRAC	2 cut(s)	372, 1274
MnlI	CCTC	7 cut(s)	7, 31, 89, 479, 779, 928, 1017
MroNI	GCCGGC	1 cut(s)	1121
MroXI	GAANNNNTTC	1 cut(s)	1247
MseI	TTAA	4 cut(s)	9, 512, 581, 1209
MslI	CAYNNNNRTG	1 cut(s)	1261
MspA1I	CMGCKG	3 cut(s)	80, 93, 1058
MspI	CCGG	6 cut(s)	199, 966, 1029, 1122, 1221, 1326
MspR9I	CCNGG	4 cut(s)	200, 668, 743, 1030
MvaI	CCWGG	2 cut(s)	668, 743
MwoI	GCNNNNNNNGC	3 cut(s)	467, 697, 1097
NaeI	GCCGGC	1 cut(s)	1123
NciI	CCSGG	2 cut(s)	200, 1030
NdeII	GATC	3 cut(s)	205, 516, 1398
NgoMIV	GCCGGC	1 cut(s)	1121
NlaIII	CATG	6 cut(s)	61, 728, 1054, 1085, 1260, 1414
NlaIV	GGNNCC	6 cut(s)	244, 319, 430, 447, 1368, 1408
NmuCI	GTSAC	2 cut(s)	604, 754
NspV	TTCGAA	1 cut(s)	900
PagI	TCATGA	3 cut(s)	724, 1050, 1081
PdiI	GCCGGC	1 cut(s)	1123
PdmI	GAANNNNTTC	1 cut(s)	1247
PfeI	GAWTC	5 cut(s)	74, 100, 764, 864, 1329
PfoI	TCCNGGA	2 cut(s)	198, 741
PkrI	GCNGC	6 cut(s)	92, 502, 685, 690, 733, 976
PleI	GAGTC	3 cut(s)	643, 751, 881
PpsI	GAGTC	3 cut(s)	643, 751, 881
PshBI	ATTAAT	1 cut(s)	581
Psp124BI	GAGCTC	1 cut(s)	1301
Psp6I	CCWGG	2 cut(s)	666, 741
PspGI	CCWGG	2 cut(s)	666, 741
PspN4I	GGNNCC	6 cut(s)	244, 319, 430, 447, 1368, 1408
PspPI	GGNCC	2 cut(s)	287, 318
PstI	CTGCAG	2 cut(s)	693, 979
PstNI	CAGNNNCTG	1 cut(s)	697
PsuI	RGATCY	2 cut(s)	205, 1398
PvuII	CAGCTG	1 cut(s)	80
RsaI	GTAC	4 cut(s)	419, 447, 1309, 1322
RsaNI	GTAC	4 cut(s)	418, 446, 1308, 1321
RseI	CAYNNNNRTG	1 cut(s)	1261
SacI	GAGCTC	1 cut(s)	1301
SaqAI	TTAA	4 cut(s)	9, 512, 581, 1209
SatI	GCNGC	6 cut(s)	91, 501, 684, 689, 732, 975
Sau3AI	GATC	3 cut(s)	205, 516, 1398
Sau96I	GGNCC	2 cut(s)	287, 318
SchI	GAGTC	3 cut(s)	644, 751, 882
ScrFI	CCNGG	4 cut(s)	200, 668, 743, 1030
SduI	GDGCHC	2 cut(s)	1301, 1371
SfaNI	GCATC	3 cut(s)	149, 700, 1034
SfcI	CTRYAG	3 cut(s)	689, 975, 1116
SfuI	TTCGAA	1 cut(s)	900
SmiMI	CAYNNNNRTG	1 cut(s)	1261
SmlI	CTYRAG	1 cut(s)	322
SmoI	CTYRAG	1 cut(s)	322
Sse9I	AATT	6 cut(s)	465, 582, 782, 831, 1011, 1085
SsiI	CCGC	3 cut(s)	93, 568, 1058
SspI	AATATT	1 cut(s)	1207
SspMI	CTAG	1 cut(s)	1238
SstI	GAGCTC	1 cut(s)	1301
StyD4I	CCNGG	4 cut(s)	198, 666, 741, 1028
StyI	CCWWGG	3 cut(s)	375, 1172, 1402
TaaI	ACNGT	1 cut(s)	1307
TaiI	ACGT	1 cut(s)	1277
TaqI	TCGA	4 cut(s)	484, 900, 1095, 1151
TasI	AATT	6 cut(s)	465, 582, 782, 831, 1011, 1085
TfiI	GAWTC	5 cut(s)	74, 100, 764, 864, 1329
Tru1I	TTAA	4 cut(s)	9, 512, 581, 1209
Tru9I	TTAA	4 cut(s)	9, 512, 581, 1209
TscAI	CASTG	2 cut(s)	240, 1395
TseFI	GTSAC	2 cut(s)	604, 754
TseI	GCWGC	6 cut(s)	90, 500, 683, 688, 731, 974
Tsp45I	GTSAC	2 cut(s)	604, 754
TspDTI	ATGAA	8 cut(s)	445, 532, 616, 741, 1053, 1070, 1098, 1303
TspGWI	ACGGA	2 cut(s)	435, 885
TspRI	CASTG	2 cut(s)	240, 1395
VspI	ATTAAT	1 cut(s)	581
XmiI	GTMKAC	1 cut(s)	1134
XmnI	GAANNNNTTC	1 cut(s)	1247
XspI	CTAG	1 cut(s)	1238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.