MD09G1127600.v1.1

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001427
Physical Location & Seq
Forward (+)
9835951 .. 9837012
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1127600.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGCGCCTCCCTGCACTCGCTGGCACGCAGCAGTCTACTTCTCCATGGCTGTAATCTTGGCCTTCATCGCAATCTCCATGACTCTGCGGTCACAACCCAACAACGAGTCCTCCCAATCCAGACCCATCAGCCGCTTCGTCTACCTCGGCGCCTCAAGAACTCTGAGACGAGCTGGCTTCACCGTCATCGCCACCCTTCTCCAGCTCTCGCCGGAACTCTTCTTCTCCTCCGCAACCACCACCCTCTTTGCCATCAAAGACTCCGCCATCTCCAACTCCTCCCTCCCGCCACGCCTTCTCAAAAACCTCCTGCGGTACCACACTTCTCCTCTCAGGCTTTCCTTCGAACACCTTGTGAACATGACTCAGGGTACTTGCTTGCCGACCCTGTATCAGAACAAGAAGATCGCAGTCACCAAAATCGATGAGAAGGAAAGATCGGTGGAAATCAATCACGTGTTGGTGTCGCACCCGAATCTGTTTCTTGACGGACCAATTTCGATTCACGGTGTTCTTGGCCCGTTCTCCGCTTTGGATCCGAGAGATGTTAATCAGGGTTGGGATATTATCCAATCGCCTGCCTGTGATGCCAATTCCAATATGGTTTCTGATGTTCCTGATGCCAAGAACATGGTTGAATGGTCCCGGATCATCCGATTGCTGAGCTCGAATGGGTTCGTTTCTTTCGCTGTCGGGCTGAACTCTGTTCTTGATGGGATTCTTGGAGATCATAAGGGTTTGAAGTCTGTGACAATCTTTCTTCCGCCAAGCTTGGAGCTTGAAGCGTATCCTTCCCCTTTGCTCGAGAAGATTGTGAGGTTTCATATTCTGCCTCAGAGGTATACAACCAGAGGGCTCGAATCGATGCCTGCCAGAACACTGCTCAGGACCCTGCTTCATGATCAGCCTCTTGAGTTCACTGGGACTCTGGATTTCATGAAAGAATTGGTCGTTAATGGAGTGAAAATCGTGGCACCTGATATCTTTTCTTCAAATAGGTTTACTGTCCATGGGATCTCCCGAGCTTTTGAGCTGGATCATCTGCCTACTCCAGTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

354

Amino Acids

39.26

Weight (kDa)

9.15

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 249 - 342 5.8e-07 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 4 cut(s) 4, 149, 315, 973
AccI GTMKAC 3 cut(s) 36, 141, 842
AciI CCGC 8 cut(s) 88, 133, 231, 264, 287, 313, 528, 764
AclWI GGATC 5 cut(s) 530, 543, 656, 1022, 1044
AcvI CACGTG 1 cut(s) 457
AcyI GRCGYC 2 cut(s) 5, 150
AdeI CACNNNGTG 1 cut(s) 355
AfaI GTAC 2 cut(s) 317, 373
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 4 cut(s) 638, 742, 782, 993
AluBI AGCT 7 cut(s) 173, 205, 666, 771, 778, 1025, 1033
AluI AGCT 7 cut(s) 173, 205, 666, 771, 778, 1025, 1033
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 5 cut(s) 530, 543, 656, 1022, 1044
Ama87I CYCGRG 2 cut(s) 803, 1020
AoxI GGCC 2 cut(s) 60, 517
ApeKI GCWGC 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 674
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 2 cut(s) 7, 152
AspS9I GGNCC 4 cut(s) 491, 518, 642, 888
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 2 cut(s) 172, 406
AsuII TTCGAA 1 cut(s) 345
AvaI CYCGRG 2 cut(s) 803, 1020
AvaII GGWCC 3 cut(s) 491, 642, 888
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 4 cut(s) 4, 149, 315, 973
BanII GRGCYC 2 cut(s) 668, 858
BbrPI CACGTG 1 cut(s) 457
Bbv12I GWGCWC 1 cut(s) 668
BbvI GCAGC 1 cut(s) 41
BccI CCATC 4 cut(s) 134, 260, 275, 707
BciVI GTATCC 1 cut(s) 798
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 1 cut(s) 160
BfoI RGCGCY 2 cut(s) 8, 153
BfuI GTATCC 1 cut(s) 798
BisI GCNGC 2 cut(s) 30, 133
BlpI GCTNAGC 1 cut(s) 662
BlsI GCNGC 2 cut(s) 31, 134
Bme1390I CCNGG 1 cut(s) 646
Bme18I GGWCC 3 cut(s) 491, 642, 888
BmeT110I CYCGRG 2 cut(s) 803, 1020
BmgT120I GGNCC 4 cut(s) 491, 518, 642, 888
BmiI GGNNCC 7 cut(s) 6, 151, 317, 537, 644, 890, 975
BmrFI CCNGG 1 cut(s) 646
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 3 cut(s) 577, 610, 855
BmuI ACTGGG 1 cut(s) 930
BpmI CTGGAG 2 cut(s) 185, 1035
Bpu10I CCTNAGC 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 662
Bpu14I TTCGAA 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 139, 932
BpuMI CCSGG 1 cut(s) 646
Bsa29I ATCGAT 2 cut(s) 423, 863
BsaAI YACGTR 1 cut(s) 457
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 2 cut(s) 5, 150
BsaJI CCNNGG 3 cut(s) 45, 145, 1009
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bse1I ACTGG 2 cut(s) 925, 1052
Bse8I GATNNNNATC 1 cut(s) 549
BseCI ATCGAT 2 cut(s) 423, 863
BseDI CCNNGG 3 cut(s) 45, 145, 1009
BseGI GGATG 1 cut(s) 651
BseJI GATNNNNATC 1 cut(s) 549
BseMII CTCAG 6 cut(s) 155, 346, 380, 653, 848, 898
BseNI ACTGG 2 cut(s) 925, 1052
BseRI GAGGAG 3 cut(s) 217, 268, 318
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 2 cut(s) 62, 519
BshNI GGYRCC 4 cut(s) 4, 149, 315, 973
BshVI ATCGAT 2 cut(s) 423, 863
BsiHKAI GWGCWC 1 cut(s) 668
BsiHKCI CYCGRG 2 cut(s) 803, 1020
BsiSI CCGG 2 cut(s) 212, 646
BslFI GGGAC 2 cut(s) 628, 937
BsmAI GTCTC 1 cut(s) 160
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 2 cut(s) 628, 937
BsnI GGCC 2 cut(s) 62, 519
BsoBI CYCGRG 2 cut(s) 803, 1020
Bsp119I TTCGAA 1 cut(s) 345
Bsp1286I GDGCHC 2 cut(s) 668, 858
Bsp143I GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
Bsp1720I GCTNAGC 1 cut(s) 662
Bsp19I CCATGG 2 cut(s) 45, 1009
BspACI CCGC 8 cut(s) 88, 133, 231, 264, 287, 313, 528, 764
BspANI GGCC 2 cut(s) 62, 519
BspCNI CTCAG 6 cut(s) 156, 345, 379, 654, 847, 897
BspDI ATCGAT 2 cut(s) 423, 863
BspHI TCATGA 2 cut(s) 898, 936
BspLI GGNNCC 7 cut(s) 6, 151, 317, 537, 644, 890, 975
BspPI GGATC 5 cut(s) 530, 543, 656, 1022, 1044
BspT104I TTCGAA 1 cut(s) 345
BspT107I GGYRCC 4 cut(s) 4, 149, 315, 973
BsrI ACTGG 2 cut(s) 925, 1052
BssECI CCNNGG 3 cut(s) 45, 145, 1009
BssMI GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
BssNAI GTATAC 1 cut(s) 843
BssNI GRCGYC 2 cut(s) 5, 150
BssT1I CCWWGG 2 cut(s) 45, 1009
Bst1107I GTATAC 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 184, 509, 1006
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 5, 150
BstBAI YACGTR 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 345
BstC8I GCNNGC 6 cut(s) 23, 27, 175, 380, 579, 870
BstDEI CTNAG 6 cut(s) 164, 332, 366, 662, 834, 884
BstDSI CCRYGG 2 cut(s) 45, 1009
BstF5I GGATG 1 cut(s) 651
BstH2I RGCGCY 2 cut(s) 8, 153
BstHHI GCGC 2 cut(s) 7, 152
BstKTI GATC 8 cut(s) 408, 440, 538, 651, 730, 904, 1017, 1039
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
BstMWI GCNNNNNNNGC 2 cut(s) 68, 587
BstSCI CCNGG 1 cut(s) 644
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 535, 1014
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 2 cut(s) 535, 1014
BstZ17I GTATAC 1 cut(s) 843
Bsu15I ATCGAT 2 cut(s) 423, 863
BsuI GTATCC 1 cut(s) 798
BsuRI GGCC 2 cut(s) 62, 519
BsuTUI ATCGAT 2 cut(s) 423, 863
BtgI CCRYGG 2 cut(s) 45, 1009
BtgZI GCGATG 2 cut(s) 52, 172
BtsCI GGATG 1 cut(s) 651
BtsI GCAGTG 1 cut(s) 878
BtsIMutI CAGTG 3 cut(s) 878, 918, 1059
Cac8I GCNNGC 6 cut(s) 23, 27, 175, 380, 579, 870
CciI TCATGA 2 cut(s) 898, 936
CfoI GCGC 2 cut(s) 7, 152
Cfr13I GGNCC 4 cut(s) 491, 518, 642, 888
ClaI ATCGAT 2 cut(s) 423, 863
Csp6I GTAC 2 cut(s) 316, 372
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 7 cut(s) 46, 79, 361, 631, 899, 937, 1010
CviQI GTAC 2 cut(s) 316, 372
DdeI CTNAG 6 cut(s) 164, 332, 366, 662, 834, 884
DinI GGCGCC 2 cut(s) 6, 151
DpnI GATC 8 cut(s) 407, 439, 537, 650, 729, 903, 1016, 1038
DpnII GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
DraIII CACNNNGTG 1 cut(s) 355
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 2 cut(s) 253, 753
Ecl136II GAGCTC 1 cut(s) 666
Eco130I CCWWGG 2 cut(s) 45, 1009
Eco24I GRGCYC 2 cut(s) 668, 858
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 3 cut(s) 491, 642, 888
Eco53kI GAGCTC 1 cut(s) 666
Eco72I CACGTG 1 cut(s) 457
Eco88I CYCGRG 2 cut(s) 803, 1020
EcoICRI GAGCTC 1 cut(s) 666
EcoO109I RGGNCCY 1 cut(s) 888
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 2 cut(s) 45, 1009
EcoT38I GRGCYC 2 cut(s) 668, 858
EgeI GGCGCC 2 cut(s) 6, 151
EheI GGCGCC 2 cut(s) 6, 151
ErhI CCWWGG 2 cut(s) 45, 1009
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 7 cut(s) 49, 82, 364, 634, 902, 940, 1013
FaqI GGGAC 2 cut(s) 628, 937
FatI CATG 7 cut(s) 45, 78, 360, 630, 898, 936, 1009
FauI CCCGC 1 cut(s) 294
FbaI TGATCA 1 cut(s) 901
FblI GTMKAC 3 cut(s) 36, 141, 842
Fnu4HI GCNGC 2 cut(s) 30, 133
FokI GGATG 1 cut(s) 638
FriOI GRGCYC 2 cut(s) 668, 858
Fsp4HI GCNGC 2 cut(s) 30, 133
GlaI GCGC 2 cut(s) 6, 151
GluI GCNGC 2 cut(s) 30, 133
GsuI CTGGAG 2 cut(s) 185, 1035
HaeII RGCGCY 2 cut(s) 8, 153
HaeIII GGCC 2 cut(s) 62, 519
HapII CCGG 2 cut(s) 212, 646
HhaI GCGC 2 cut(s) 7, 152
Hin1I GRCGYC 2 cut(s) 5, 150
Hin1II CATG 7 cut(s) 49, 82, 364, 634, 902, 940, 1013
Hin6I GCGC 2 cut(s) 5, 150
HinP1I GCGC 2 cut(s) 5, 150
HindIII AAGCTT 1 cut(s) 769
HinfI GANTC 9 cut(s) 82, 107, 260, 364, 475, 502, 718, 860, 925
HpaII CCGG 2 cut(s) 212, 646
HphI GGTGA 2 cut(s) 172, 406
Hpy166II GTNNAC 6 cut(s) 37, 142, 358, 843, 918, 1002
Hpy188I TCNGA 6 cut(s) 165, 396, 540, 610, 656, 837
Hpy8I GTNNAC 6 cut(s) 37, 142, 358, 843, 918, 1002
HpyAV CCTTC 6 cut(s) 73, 206, 305, 352, 424, 801
HpyCH4III ACNGT 3 cut(s) 184, 509, 1006
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 587
HpyF3I CTNAG 6 cut(s) 164, 332, 366, 662, 834, 884
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 2 cut(s) 5, 150
Hsp92II CATG 7 cut(s) 49, 82, 364, 634, 902, 940, 1013
HspAI GCGC 2 cut(s) 5, 150
KasI GGCGCC 2 cut(s) 4, 149
KpnI GGTACC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 901
Kzo9I GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 3 cut(s) 577, 610, 855
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 3 cut(s) 90, 412, 748
MalI GATC 8 cut(s) 407, 439, 537, 650, 729, 903, 1016, 1038
MboI GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
MboII GAAGA 6 cut(s) 211, 214, 415, 752, 820, 981
MflI RGATCY 2 cut(s) 535, 1014
MhlI GDGCHC 2 cut(s) 668, 858
MluCI AATT 3 cut(s) 495, 592, 944
Mly113I GGCGCC 2 cut(s) 5, 150
MlyI GAGTC 5 cut(s) 76, 116, 254, 358, 919
MmeI TCCRAC 1 cut(s) 297
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 2 cut(s) 549, 954
MspI CCGG 2 cut(s) 212, 646
MspR9I CCNGG 1 cut(s) 646
MwoI GCNNNNNNNGC 2 cut(s) 68, 587
NarI GGCGCC 2 cut(s) 5, 150
NciI CCSGG 1 cut(s) 646
NcoI CCATGG 2 cut(s) 45, 1009
NdeII GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
NlaIII CATG 7 cut(s) 49, 82, 364, 634, 902, 940, 1013
NlaIV GGNNCC 7 cut(s) 6, 151, 317, 537, 644, 890, 975
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 3 cut(s) 90, 412, 748
NspV TTCGAA 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 803
PagI TCATGA 2 cut(s) 898, 936
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 4 cut(s) 475, 502, 718, 860
PfoI TCCNGGA 1 cut(s) 644
PkrI GCNGC 2 cut(s) 31, 134
PleI GAGTC 5 cut(s) 76, 115, 254, 358, 919
PluTI GGCGCC 2 cut(s) 8, 153
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
PpsI GAGTC 5 cut(s) 76, 115, 254, 358, 919
Ppu21I YACGTR 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 888
Psp124BI GAGCTC 1 cut(s) 668
Psp5II RGGWCCY 1 cut(s) 888
PspCI CACGTG 1 cut(s) 457
PspN4I GGNNCC 7 cut(s) 6, 151, 317, 537, 644, 890, 975
PspPI GGNCC 4 cut(s) 491, 518, 642, 888
PspPPI RGGWCCY 1 cut(s) 888
PsuI RGATCY 2 cut(s) 535, 1014
RsaI GTAC 2 cut(s) 317, 373
RsaNI GTAC 2 cut(s) 316, 372
SacI GAGCTC 1 cut(s) 668
SaqAI TTAA 2 cut(s) 549, 954
SatI GCNGC 2 cut(s) 30, 133
Sau3AI GATC 8 cut(s) 405, 437, 535, 648, 727, 901, 1014, 1036
Sau96I GGNCC 4 cut(s) 491, 518, 642, 888
SchI GAGTC 5 cut(s) 76, 116, 254, 358, 919
ScrFI CCNGG 1 cut(s) 646
SduI GDGCHC 2 cut(s) 668, 858
SfaNI GCATC 3 cut(s) 577, 610, 855
SfoI GGCGCC 2 cut(s) 6, 151
Sfr274I CTCGAG 1 cut(s) 803
SfuI TTCGAA 1 cut(s) 345
SinI GGWCC 3 cut(s) 491, 642, 888
SlaI CTCGAG 1 cut(s) 803
SmlI CTYRAG 3 cut(s) 154, 803, 911
SmoI CTYRAG 3 cut(s) 154, 803, 911
Sse9I AATT 3 cut(s) 495, 592, 944
SsiI CCGC 8 cut(s) 88, 133, 231, 264, 287, 313, 528, 764
SspDI GGCGCC 2 cut(s) 4, 149
SstI GAGCTC 1 cut(s) 668
StyD4I CCNGG 1 cut(s) 644
StyI CCWWGG 2 cut(s) 45, 1009
TaaI ACNGT 3 cut(s) 184, 509, 1006
TaiI ACGT 1 cut(s) 459
TaqI TCGA 7 cut(s) 345, 423, 500, 668, 804, 858, 863
TasI AATT 3 cut(s) 495, 592, 944
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 4 cut(s) 475, 502, 718, 860
Tru1I TTAA 2 cut(s) 549, 954
Tru9I TTAA 2 cut(s) 549, 954
TscAI CASTG 3 cut(s) 885, 925, 1059
TseFI GTSAC 3 cut(s) 90, 412, 748
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 3 cut(s) 90, 412, 748
TspDTI ATGAA 5 cut(s) 55, 812, 887, 925, 953
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 3 cut(s) 885, 925, 1059
VpaK11BI GGWCC 3 cut(s) 491, 642, 888
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XhoI CTCGAG 1 cut(s) 803
XmiI GTMKAC 3 cut(s) 36, 141, 842
XmnI GAANNNNTTC 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.