MD12G1092600.v1.1

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000239
Physical Location & Seq
Forward (+)
11878625 .. 11881357
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1092600.v1.1.491

Sequence Viewer

Length: 852 bp
ATGTACTTTAAAAAAAAAATTTATAAGTGCATTCATGTTGATCACCATAGTTATCAAGTAAAACAAGTTTTAATTTCTGCCCTGCTGCAGGGTTGGTACCACTACAGCGTTTTGTTAGACGGTTTGGGCGATGGGATCTTTGCCGTGGATGGACTAAAATGGCTGCATCAGAGGAAGATATCGAGCCATGTGTTATCAACCAAAATTGTCAAAGACTTCAGCACTACAGTCTTTAAAACCAACGTGGTACAAATTGCCGGGATAATTTCTGAAGCTGCAACCTGCGACAAAGCAATAGAGATCCAAATCAGACGGTTCTTAAACATTGGATCAGAAGCAGTGCTGAAGAAGAATATCAAGGTGATAGATCAGTTTATTTATAATTTAATCAATACCAAGACTGAAACGCTCCATAATTCACCAGAAGATGATCTGTCTGCAGTGAGTTACCTTCCAGTTAGAGAGGCAACAAATCTGAAAGATAGTTCAAGCATTGATGAGGTTGCAGCCAGCCTTACTGACGATGCTCTTGACAAAATGCATTATCTCCATGCATCTTTGTCTGAGACACTCAGGCTCCACCCTGCAGTTCCACTGGATGCCTGGGTTTGTTTAGCTGATGATACATGGCCAGATGGATTTAGTGTCAAGAAAGGGAGTTTTGCGGGACCAAGAAATTGTATAGGGAAAGACTTTGCTTATCGGCAGGCAAAGATCGTTTGTGCAATGCTTTTGAGGAGCTACATATTCAAGCTGAGTGATGAGAACAAAGTGGCTCATTACCGGACCAAGATCACCCTCCATATTGATGGGGGACTTTATGTGCAAGCCTTTCCAAGAAACCTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

284

Amino Acids

31.92

Weight (kDa)

8.64

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 171 - 220 3.1e-06 Cytochrome P450
p450 PF00067 222 - 260 7.7e-06 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000239)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12320 FvH4_7g28340 FvH4_7g28350 FvH4_7g28350 FvH4_7g28361 FvH4_7g28450 FvH4_7g28450 FvH4_7g28452 FvH4_7g28453 FvH4_7g28453 FvH4_7g28454 FvH4_7g28455 FvH4_7g28458
malus_domestica MD01G1188000.v1.1 MD01G1188200.v1.1 MD02G1199800.v1.1 MD07G1191000.v1.1 MD11G1075700.v1.1 MD12G1092600.v1.1
prunus_persica Prupe.2G148800_v2.0.a1 Prupe.2G284500_v2.0.a1 Prupe.2G284800_v2.0.a1 Prupe.2G285200_v2.0.a1 Prupe.2G285200_v2.0.a1 Prupe.2G285200_v2.0.a1 Prupe.2G285200_v2.0.a1
pyrus_communis pycom02g16380 pycom11g06340
rosa_chinensis RchiOBHm_Chr1g0351121 RchiOBHm_Chr1g0359881 RchiOBHm_Chr1g0359951 RchiOBHm_Chr1g0375301 RchiOBHm_Chr1g0375441 RchiOBHm_Chr1g0375471 RchiOBHm_Chr1g0375491 RchiOBHm_Chr1g0375521 RchiOBHm_Chr1g0375531 RchiOBHm_Chr1g0375571 RchiOBHm_Chr1g0375641 RchiOBHm_Chr1g0375681 RchiOBHm_Chr1g0375701 RchiOBHm_Chr1g0375721 RchiOBHm_Chr1g0375751 RchiOBHm_Chr1g0375811 RchiOBHm_Chr1g0375831 RchiOBHm_Chr2g0116601 RchiOBHm_Chr2g0143131
rosa_laevigata RLG00000018292 RLG00000026650 RLG00000026653 RLG00000026655 RLG00000026657 RLG00000026659 RLG00000026660 RLG00000026662 RLG00000026670 RLG00000027846 RLG00000028430
rosa_multiflora Rmu_co8068694.1_g000001 Rmu_co8138120.1_g000001 Rmu_co8293475.1_g000001 Rmu_sc0000487.1_g000028 Rmu_sc0000487.1_g000030 Rmu_sc0000487.1_g000031 Rmu_sc0000487.1_g000051 Rmu_sc0000554.1_g000038 Rmu_sc0001619.1_g000015 Rmu_sc0001644.1_g000001 Rmu_sc0001644.1_g000009 Rmu_sc0001644.1_g000012 Rmu_sc0001644.1_g000013 Rmu_sc0001644.1_g000020 Rmu_sc0001644.1_g000023 Rmu_sc0001644.1_g000031 Rmu_sc0001943.1_g000001 Rmu_sc0001943.1_g000005 Rmu_sc0006087.1_g000005 Rmu_sc0008800.1_g000004 Rmu_sc0015155.1_g000001 Rmu_ssc0000106.1_g000005 Rmu_ssc0000368.1_g000003
rosa_roxburghii Rroxscaffold_2G00102100 Rroxscaffold_2G00127030 Rroxscaffold_4G00282580 Rroxscaffold_4G00282620 Rroxscaffold_4G00282650 Rroxscaffold_4G00282670 Rroxscaffold_4G00282740 Rroxscaffold_4G00282840 Rroxscaffold_4G00304050
rosa_rugosa Rorug01G0216600 Rorug01G0273300 Rorug01G0273400 Rorug01G0390900 Rorug01G0391000 Rorug01G0391400 Rorug01G0391500 Rorug01G0391600 Rorug01G0391700 Rorug01G0391800 Rorug02G0061300 Rorug02G0207800 Rorug02G0378000 Rorug02G0378100 Rorug02G0378200 Rorug02G0378300 Rorug02G0378300 Rorug02G0378300 Rorug03G0168600 Rorug06G0412800 Rorug06G0412900 Rorug06G0413000
rosa_samantha Rh1AG231000 Rh1AG287500 Rh1AG402800 Rh1CG379000 Rh2BG440100 Rh2CG417400 Rh2DG271100
rosa_wichuraiana Rw0G023590 Rw0G023600 Rw1G020080 Rw1G025530 Rw1G025540 Rw1G035580 Rw1G035650 Rw1G035670 Rw1G035690 Rw1G035700 Rw1G035740 Rw1G035760 Rw1G035780 Rw1G035800 Rw2G020700 Rw2G035250 Rw4G005840 Rw4G005850 Rw4G005860 Rw4G005890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 24, 381
Acc36I ACCTGC 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AciI CCGC 1 cut(s) 665
AclWI GGATC 3 cut(s) 143, 295, 337
AcoI YGGCCR 1 cut(s) 629
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 3 cut(s) 202, 291, 365
AfaI GTAC 3 cut(s) 5, 98, 249
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 2 cut(s) 489, 751
AjnI CCWGG 1 cut(s) 602
AluBI AGCT 4 cut(s) 275, 617, 741, 754
AluI AGCT 4 cut(s) 275, 617, 741, 754
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 3 cut(s) 143, 295, 337
AoxI GGCC 1 cut(s) 629
ApeKI GCWGC 4 cut(s) 85, 163, 275, 506
ApoI RAATTY 1 cut(s) 18
Asp718I GGTACC 1 cut(s) 96
AspS9I GGNCC 2 cut(s) 668, 786
AsuC2I CCSGG 1 cut(s) 259
AsuHPI GGTGA 4 cut(s) 35, 373, 411, 787
AvaII GGWCC 2 cut(s) 668, 786
BalI TGGCCA 1 cut(s) 631
BanI GGYRCC 1 cut(s) 96
BbvI GCAGC 4 cut(s) 72, 150, 262, 518
BccI CCATC 4 cut(s) 125, 143, 629, 803
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 604
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 560
BfmI CTRYAG 5 cut(s) 86, 103, 225, 438, 585
BfuAI ACCTGC 1 cut(s) 290
BisI GCNGC 4 cut(s) 86, 164, 276, 507
BlsI GCNGC 4 cut(s) 87, 165, 277, 508
Bme1390I CCNGG 2 cut(s) 259, 604
Bme18I GGWCC 2 cut(s) 668, 786
BmgT120I GGNCC 2 cut(s) 668, 786
BmiI GGNNCC 3 cut(s) 98, 578, 669
BmrFI CCNGG 2 cut(s) 259, 604
BmsI GCATC 4 cut(s) 175, 514, 563, 589
BpuMI CCSGG 1 cut(s) 259
BsaBI GATNNNNATC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 144, 603
BsaWI WCCGGW 1 cut(s) 783
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 455, 600
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 305
BseBI CCWGG 1 cut(s) 604
BseDI CCNNGG 2 cut(s) 144, 603
BseGI GGATG 2 cut(s) 154, 604
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 3 cut(s) 555, 586, 746
BseNI ACTGG 2 cut(s) 455, 600
BseRI GAGGAG 1 cut(s) 751
BseXI GCAGC 4 cut(s) 72, 150, 262, 518
BshFI GGCC 1 cut(s) 631
BshNI GGYRCC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 258, 784
BslFI GGGAC 2 cut(s) 681, 828
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 2 cut(s) 681, 828
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 1 cut(s) 631
Bsp143I GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
BspACI CCGC 1 cut(s) 665
BspANI GGCC 1 cut(s) 631
BspCNI CTCAG 3 cut(s) 556, 585, 747
BspLI GGNNCC 3 cut(s) 98, 578, 669
BspMAI CTGCAG 3 cut(s) 90, 442, 589
BspMI ACCTGC 1 cut(s) 290
BspPI GGATC 3 cut(s) 143, 295, 337
BspT107I GGYRCC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 2 cut(s) 455, 600
BssECI CCNNGG 2 cut(s) 144, 603
BssMI GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 3 cut(s) 122, 229, 315
BstC8I GCNNGC 3 cut(s) 511, 708, 828
BstDEI CTNAG 3 cut(s) 564, 572, 755
BstDSI CCRYGG 1 cut(s) 144
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 2 cut(s) 154, 604
BstKTI GATC 8 cut(s) 43, 138, 303, 332, 370, 433, 717, 795
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
BstNI CCWGG 1 cut(s) 604
BstSCI CCNGG 2 cut(s) 257, 602
BstSFI CTRYAG 5 cut(s) 86, 103, 225, 438, 585
BstV1I GCAGC 4 cut(s) 72, 150, 262, 518
BstX2I RGATCY 2 cut(s) 135, 300
BstXI CCANNNNNNTGG 1 cut(s) 809
BstYI RGATCY 2 cut(s) 135, 300
BsuRI GGCC 1 cut(s) 631
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 144
BtsCI GGATG 2 cut(s) 154, 604
BtsI GCAGTG 2 cut(s) 345, 447
BtsIMutI CAGTG 3 cut(s) 345, 447, 593
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 3 cut(s) 511, 708, 828
Cfr13I GGNCC 2 cut(s) 668, 786
Csp6I GTAC 3 cut(s) 4, 97, 248
CviAII CATG 4 cut(s) 35, 188, 551, 627
CviQI GTAC 3 cut(s) 4, 97, 248
DdeI CTNAG 3 cut(s) 564, 572, 755
DpnI GATC 8 cut(s) 42, 137, 302, 331, 369, 432, 716, 794
DpnII GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
DraI TTTAAA 2 cut(s) 10, 235
EaeI YGGCCR 1 cut(s) 629
Eco32I GATATC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 668, 786
Eco57I CTGAAG 3 cut(s) 202, 291, 365
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 602
EcoRV GATATC 1 cut(s) 180
EcoT22I ATGCAT 2 cut(s) 543, 556
FaeI CATG 4 cut(s) 38, 191, 554, 630
FaqI GGGAC 2 cut(s) 681, 828
FatI CATG 4 cut(s) 34, 187, 550, 626
FauI CCCGC 1 cut(s) 658
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 4 cut(s) 86, 164, 276, 507
FokI GGATG 2 cut(s) 161, 611
Fsp4HI GCNGC 4 cut(s) 86, 164, 276, 507
GluI GCNGC 4 cut(s) 86, 164, 276, 507
HaeIII GGCC 1 cut(s) 631
HapII CCGG 2 cut(s) 258, 784
Hin1II CATG 4 cut(s) 38, 191, 554, 630
HpaII CCGG 2 cut(s) 258, 784
HphI GGTGA 4 cut(s) 35, 373, 411, 787
Hpy188I TCNGA 6 cut(s) 171, 271, 311, 334, 477, 565
Hpy188III TCNNGA 2 cut(s) 530, 649
HpyAV CCTTC 1 cut(s) 461
HpyCH4III ACNGT 3 cut(s) 122, 229, 315
HpyCH4IV ACGT 1 cut(s) 243
HpyF3I CTNAG 3 cut(s) 564, 572, 755
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 4 cut(s) 38, 191, 554, 630
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
LmnI GCTCC 3 cut(s) 414, 582, 738
Lsp1109I GCAGC 4 cut(s) 72, 150, 262, 518
LweI GCATC 4 cut(s) 175, 514, 563, 589
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 446
MalI GATC 8 cut(s) 42, 137, 302, 331, 369, 432, 716, 794
MboI GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
MboII GAAGA 4 cut(s) 187, 358, 361, 437
MflI RGATCY 2 cut(s) 135, 300
MlsI TGGCCA 1 cut(s) 631
MluCI AATT 9 cut(s) 18, 72, 204, 252, 264, 382, 415, 676, 847
MluNI TGGCCA 1 cut(s) 631
MnlI CCTC 5 cut(s) 165, 457, 493, 729, 809
Mox20I TGGCCA 1 cut(s) 631
Mph1103I ATGCAT 2 cut(s) 543, 556
MscI TGGCCA 1 cut(s) 631
MseI TTAA 6 cut(s) 9, 71, 234, 320, 386, 846
MslI CAYNNNNRTG 1 cut(s) 807
Msp20I TGGCCA 1 cut(s) 631
MspI CCGG 2 cut(s) 258, 784
MspR9I CCNGG 2 cut(s) 259, 604
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 1 cut(s) 604
NciI CCSGG 1 cut(s) 259
NdeII GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
NlaIII CATG 4 cut(s) 38, 191, 554, 630
NlaIV GGNNCC 3 cut(s) 98, 578, 669
NsiI ATGCAT 2 cut(s) 543, 556
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 30
PkrI GCNGC 4 cut(s) 87, 165, 277, 508
PsiI TTATAA 2 cut(s) 24, 381
Psp6I CCWGG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 3 cut(s) 98, 578, 669
PspPI GGNCC 2 cut(s) 668, 786
PstI CTGCAG 3 cut(s) 90, 442, 589
PsuI RGATCY 2 cut(s) 135, 300
RsaI GTAC 3 cut(s) 5, 98, 249
RsaNI GTAC 3 cut(s) 4, 97, 248
RseI CAYNNNNRTG 1 cut(s) 807
SaqAI TTAA 6 cut(s) 9, 71, 234, 320, 386, 846
SatI GCNGC 4 cut(s) 86, 164, 276, 507
Sau3AI GATC 8 cut(s) 40, 135, 300, 329, 367, 430, 714, 792
Sau96I GGNCC 2 cut(s) 668, 786
ScrFI CCNGG 2 cut(s) 259, 604
SfaNI GCATC 4 cut(s) 175, 514, 563, 589
SfcI CTRYAG 5 cut(s) 86, 103, 225, 438, 585
SinI GGWCC 2 cut(s) 668, 786
SmiMI CAYNNNNRTG 1 cut(s) 807
Sse9I AATT 9 cut(s) 18, 72, 204, 252, 264, 382, 415, 676, 847
SsiI CCGC 1 cut(s) 665
StyD4I CCNGG 2 cut(s) 257, 602
TaaI ACNGT 3 cut(s) 122, 229, 315
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 182
TasI AATT 9 cut(s) 18, 72, 204, 252, 264, 382, 415, 676, 847
TatI WGTACW 1 cut(s) 3
Tru1I TTAA 6 cut(s) 9, 71, 234, 320, 386, 846
Tru9I TTAA 6 cut(s) 9, 71, 234, 320, 386, 846
TscAI CASTG 3 cut(s) 345, 447, 600
TseI GCWGC 4 cut(s) 85, 163, 275, 506
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 3 cut(s) 345, 447, 600
VpaK11BI GGWCC 2 cut(s) 668, 786
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 18
XcmI CCANNNNNNNNNTGG 1 cut(s) 600
Zsp2I ATGCAT 2 cut(s) 543, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.