Rroxscaffold_4G00282620

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000239
Physical Location & Seq
Forward (+)
4692486 .. 4694673
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282620.1

Sequence Viewer

Length: 561 bp
ATGATCGCGGGCGTTCTTCGAAGGTTGCTCCTTCGGGATAGGCTTCGGAATGAAAGCCTCGTTTCACTTGCGGCGCTAGAGGCAACGGTAGTAGTGACATCCTGGCCCTCGGCGGCGGGCGAGCGCATCTTGTCGGATGGATTAGGCGATGGGATCTTCGCGGTGGATGGGCAAAAATGGCAGCATCGGAGGAAGGTGATGAGCGCCGAGTTCTCAACAAAAGTAGTGAGAGACTTCAGCAGTGCTGTTTTCAAAACCAATGTAGTTAAACTTGCTGGCATAATTTATGAAGCTACAACCAACAACCCGGCAATAGAGATCCAAGATTTGTTTATGAAATCAACCTTGGATTCAATCATCAAGATTCTACTTGGTATCGAAATAGATAGCATGAGTGGAAAAGATGACGAAGGTAACCGGTTTTCCCATGCTTTTGAATCGGGGCAAGAAGCTACCTGTATCGGTTGTTTGATATGTTCGGAAGATCAAACGCTTCTTAAACATCGGAAAGGAAGCAGAGCTAAAGAAAAACATCAAAGAGATCGATCAATTTTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

186

Amino Acids

20.61

Weight (kDa)

8.46

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 136 1e-06 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000239)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12320 FvH4_7g28340 FvH4_7g28350 FvH4_7g28350 FvH4_7g28361 FvH4_7g28450 FvH4_7g28450 FvH4_7g28452 FvH4_7g28453 FvH4_7g28453 FvH4_7g28454 FvH4_7g28455 FvH4_7g28458
malus_domestica MD01G1188000.v1.1 MD01G1188200.v1.1 MD02G1199800.v1.1 MD07G1191000.v1.1 MD11G1075700.v1.1 MD12G1092600.v1.1
prunus_persica Prupe.2G148800_v2.0.a1 Prupe.2G284500_v2.0.a1 Prupe.2G284800_v2.0.a1 Prupe.2G285200_v2.0.a1 Prupe.2G285200_v2.0.a1 Prupe.2G285200_v2.0.a1 Prupe.2G285200_v2.0.a1
pyrus_communis pycom02g16380 pycom11g06340
rosa_chinensis RchiOBHm_Chr1g0351121 RchiOBHm_Chr1g0359881 RchiOBHm_Chr1g0359951 RchiOBHm_Chr1g0375301 RchiOBHm_Chr1g0375441 RchiOBHm_Chr1g0375471 RchiOBHm_Chr1g0375491 RchiOBHm_Chr1g0375521 RchiOBHm_Chr1g0375531 RchiOBHm_Chr1g0375571 RchiOBHm_Chr1g0375641 RchiOBHm_Chr1g0375681 RchiOBHm_Chr1g0375701 RchiOBHm_Chr1g0375721 RchiOBHm_Chr1g0375751 RchiOBHm_Chr1g0375811 RchiOBHm_Chr1g0375831 RchiOBHm_Chr2g0116601 RchiOBHm_Chr2g0143131
rosa_laevigata RLG00000018292 RLG00000026650 RLG00000026653 RLG00000026655 RLG00000026657 RLG00000026659 RLG00000026660 RLG00000026662 RLG00000026670 RLG00000027846 RLG00000028430
rosa_multiflora Rmu_co8068694.1_g000001 Rmu_co8138120.1_g000001 Rmu_co8293475.1_g000001 Rmu_sc0000487.1_g000028 Rmu_sc0000487.1_g000030 Rmu_sc0000487.1_g000031 Rmu_sc0000487.1_g000051 Rmu_sc0000554.1_g000038 Rmu_sc0001619.1_g000015 Rmu_sc0001644.1_g000001 Rmu_sc0001644.1_g000009 Rmu_sc0001644.1_g000012 Rmu_sc0001644.1_g000013 Rmu_sc0001644.1_g000020 Rmu_sc0001644.1_g000023 Rmu_sc0001644.1_g000031 Rmu_sc0001943.1_g000001 Rmu_sc0001943.1_g000005 Rmu_sc0006087.1_g000005 Rmu_sc0008800.1_g000004 Rmu_sc0015155.1_g000001 Rmu_ssc0000106.1_g000005 Rmu_ssc0000368.1_g000003
rosa_roxburghii Rroxscaffold_2G00102100 Rroxscaffold_2G00127030 Rroxscaffold_4G00282580 Rroxscaffold_4G00282620 Rroxscaffold_4G00282650 Rroxscaffold_4G00282670 Rroxscaffold_4G00282740 Rroxscaffold_4G00282840 Rroxscaffold_4G00304050
rosa_rugosa Rorug01G0216600 Rorug01G0273300 Rorug01G0273400 Rorug01G0390900 Rorug01G0391000 Rorug01G0391400 Rorug01G0391500 Rorug01G0391600 Rorug01G0391700 Rorug01G0391800 Rorug02G0061300 Rorug02G0207800 Rorug02G0378000 Rorug02G0378100 Rorug02G0378200 Rorug02G0378300 Rorug02G0378300 Rorug02G0378300 Rorug03G0168600 Rorug06G0412800 Rorug06G0412900 Rorug06G0413000
rosa_samantha Rh1AG231000 Rh1AG287500 Rh1AG402800 Rh1CG379000 Rh2BG440100 Rh2CG417400 Rh2DG271100
rosa_wichuraiana Rw0G023590 Rw0G023600 Rw1G020080 Rw1G025530 Rw1G025540 Rw1G035580 Rw1G035650 Rw1G035670 Rw1G035690 Rw1G035700 Rw1G035740 Rw1G035760 Rw1G035780 Rw1G035800 Rw2G020700 Rw2G035250 Rw4G005840 Rw4G005850 Rw4G005860 Rw4G005890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 8, 161
AciI CCGC 5 cut(s) 8, 71, 113, 116, 161
AclWI GGATC 2 cut(s) 161, 313
AcuI CTGAAG 1 cut(s) 220
AgeI ACCGGT 1 cut(s) 417
AgsI TTSAA 3 cut(s) 253, 354, 437
AjnI CCWGG 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 3 cut(s) 293, 452, 521
AluI AGCT 3 cut(s) 293, 452, 521
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 2 cut(s) 161, 313
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 181
AsiGI ACCGGT 1 cut(s) 417
AspLEI GCGC 3 cut(s) 76, 126, 206
AspS9I GGNCC 1 cut(s) 105
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 1 cut(s) 208
AsuII TTCGAA 1 cut(s) 19
BbvI GCAGC 1 cut(s) 193
BccI CCATC 3 cut(s) 131, 143, 161
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 2 cut(s) 77, 207
BisI GCNGC 3 cut(s) 72, 114, 182
BlsI GCNGC 3 cut(s) 73, 115, 183
Bme1390I CCNGG 2 cut(s) 103, 308
BmgT120I GGNCC 1 cut(s) 105
BmrFI CCNGG 2 cut(s) 103, 308
BmsI GCATC 2 cut(s) 135, 193
Bpu14I TTCGAA 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 108, 345
BsaWI WCCGGW 1 cut(s) 417
Bse118I RCCGGY 1 cut(s) 417
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 544
BseDI CCNNGG 2 cut(s) 108, 345
BseGI GGATG 3 cut(s) 98, 142, 172
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 8, 161
BshFI GGCC 1 cut(s) 106
BshTI ACCGGT 1 cut(s) 417
BshVI ATCGAT 1 cut(s) 544
BsiSI CCGG 2 cut(s) 308, 418
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 1 cut(s) 106
Bsp119I TTCGAA 1 cut(s) 19
Bsp143I GATC 6 cut(s) 3, 153, 318, 484, 541, 545
BspACI CCGC 5 cut(s) 8, 71, 113, 116, 161
BspANI GGCC 1 cut(s) 106
BspDI ATCGAT 1 cut(s) 544
BspFNI CGCG 2 cut(s) 8, 161
BspPI GGATC 2 cut(s) 161, 313
BspT104I TTCGAA 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 417
BssAI RCCGGY 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 108, 345
BssMI GATC 6 cut(s) 3, 153, 318, 484, 541, 545
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 19
BstC8I GCNNGC 4 cut(s) 10, 118, 122, 277
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 3 cut(s) 98, 142, 172
BstFNI CGCG 2 cut(s) 8, 161
BstH2I RGCGCY 2 cut(s) 77, 207
BstHHI GCGC 3 cut(s) 76, 126, 206
BstKTI GATC 6 cut(s) 6, 156, 321, 487, 544, 548
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 6 cut(s) 3, 153, 318, 484, 541, 545
BstMWI GCNNNNNNNGC 2 cut(s) 80, 178
BstNI CCWGG 1 cut(s) 103
BstPI GGTNACC 1 cut(s) 413
BstSCI CCNGG 2 cut(s) 101, 306
BstUI CGCG 2 cut(s) 8, 161
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 153, 318
BstYI RGATCY 2 cut(s) 153, 318
Bsu15I ATCGAT 1 cut(s) 544
BsuRI GGCC 1 cut(s) 106
BsuTUI ATCGAT 1 cut(s) 544
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 3 cut(s) 98, 142, 172
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 4 cut(s) 10, 118, 122, 277
CfoI GCGC 3 cut(s) 76, 126, 206
Cfr10I RCCGGY 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 105
ClaI ATCGAT 1 cut(s) 544
CspAI ACCGGT 1 cut(s) 417
CviAII CATG 2 cut(s) 391, 428
CviJI RGCY 6 cut(s) 43, 57, 106, 293, 452, 521
CviKI_1 RGCY 6 cut(s) 43, 57, 106, 293, 452, 521
DpnI GATC 6 cut(s) 5, 155, 320, 486, 543, 547
DpnII GATC 6 cut(s) 3, 153, 318, 484, 541, 545
Eco130I CCWWGG 1 cut(s) 345
Eco57I CTGAAG 1 cut(s) 220
Eco91I GGTNACC 1 cut(s) 413
EcoO65I GGTNACC 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 2 cut(s) 394, 431
FaiI YATR 7 cut(s) 281, 288, 335, 392, 429, 475, 559
FatI CATG 2 cut(s) 390, 427
FauI CCCGC 1 cut(s) 109
Fnu4HI GCNGC 3 cut(s) 72, 114, 182
FokI GGATG 3 cut(s) 85, 149, 179
Fsp4HI GCNGC 3 cut(s) 72, 114, 182
FspBI CTAG 1 cut(s) 77
GlaI GCGC 3 cut(s) 75, 125, 205
GluI GCNGC 3 cut(s) 72, 114, 182
HaeII RGCGCY 2 cut(s) 77, 207
HaeIII GGCC 1 cut(s) 106
HapII CCGG 2 cut(s) 308, 418
HhaI GCGC 3 cut(s) 76, 126, 206
Hin1II CATG 2 cut(s) 394, 431
Hin6I GCGC 3 cut(s) 74, 124, 204
HinP1I GCGC 3 cut(s) 74, 124, 204
HinfI GANTC 3 cut(s) 350, 364, 437
HpaII CCGG 2 cut(s) 308, 418
HphI GGTGA 1 cut(s) 208
Hpy188I TCNGA 5 cut(s) 48, 136, 189, 481, 507
Hpy188III TCNNGA 2 cut(s) 35, 361
HpyAV CCTTC 4 cut(s) 15, 41, 187, 404
HpyCH4III ACNGT 1 cut(s) 88
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 178
Hsp92II CATG 2 cut(s) 394, 431
HspAI GCGC 3 cut(s) 74, 124, 204
Kzo9I GATC 6 cut(s) 3, 153, 318, 484, 541, 545
LmnI GCTCC 1 cut(s) 33
LpnPI CCDG 6 cut(s) 88, 115, 261, 321, 431, 469
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 135, 193
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 2 cut(s) 94, 413
MalI GATC 6 cut(s) 5, 155, 320, 486, 543, 547
MboI GATC 6 cut(s) 3, 153, 318, 484, 541, 545
MboII GAAGA 3 cut(s) 8, 148, 494
MflI RGATCY 2 cut(s) 153, 318
MluCI AATT 2 cut(s) 282, 549
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 68, 73, 118, 183
MseI TTAA 2 cut(s) 267, 498
MspI CCGG 2 cut(s) 308, 418
MspR9I CCNGG 2 cut(s) 103, 308
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 2 cut(s) 8, 161
MwoI GCNNNNNNNGC 2 cut(s) 80, 178
NciI CCSGG 1 cut(s) 308
NdeII GATC 6 cut(s) 3, 153, 318, 484, 541, 545
NlaIII CATG 2 cut(s) 394, 431
NmeAIII GCCGAG 2 cut(s) 89, 232
NmuCI GTSAC 1 cut(s) 94
NspV TTCGAA 1 cut(s) 19
PfeI GAWTC 3 cut(s) 350, 364, 437
PinAI ACCGGT 1 cut(s) 417
PkrI GCNGC 3 cut(s) 73, 115, 183
Psp6I CCWGG 1 cut(s) 101
PspEI GGTNACC 1 cut(s) 413
PspGI CCWGG 1 cut(s) 101
PspPI GGNCC 1 cut(s) 105
PsuI RGATCY 2 cut(s) 153, 318
SaqAI TTAA 2 cut(s) 267, 498
SatI GCNGC 3 cut(s) 72, 114, 182
Sau3AI GATC 6 cut(s) 3, 153, 318, 484, 541, 545
Sau96I GGNCC 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 103, 308
SetI ASST 8 cut(s) 26, 198, 295, 347, 415, 454, 458, 523
SfaNI GCATC 2 cut(s) 135, 193
SfuI TTCGAA 1 cut(s) 19
Sse9I AATT 2 cut(s) 282, 549
SsiI CCGC 5 cut(s) 8, 71, 113, 116, 161
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 101, 306
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 88
TaqI TCGA 3 cut(s) 19, 378, 544
TasI AATT 2 cut(s) 282, 549
TauI GCSGC 2 cut(s) 74, 116
TfiI GAWTC 3 cut(s) 350, 364, 437
Tru1I TTAA 2 cut(s) 267, 498
Tru9I TTAA 2 cut(s) 267, 498
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 3 cut(s) 66, 303, 350
TspRI CASTG 1 cut(s) 247
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.