Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001366

Physical Location & Seq

Genomic Coordinates Reverse (-)

12300449 .. 12303229

Gene Structure 2781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1163200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGATGTCGGTGGCGTCGCCGAGAAAATCAATAACAGAGAAGGTGTTTGAGAGGGTTGGCGGGTATTCTTACCTGAGCAGCTACCTCAAGAACAACGGCGGCAGCCACAGCAGCCAGAGACACCACCGCCACAACCACCACAAGGCGTGCGAGAACGACGAGAGTGATGACGATTACTACGAGGATGAGGGGACGGTGGAGCTCGTGCAGATTGGAGCTGAGAGGACCAAGAACGTCTTGATTCTCATGAGCGACACCGGCGGCGGCCACCGCGCTTCCTCCGAGGCCATTCGTGATGCCTTCCGCATGGAGTTTGGCGATGAATACAGGATATTTGTGAAGGATGTGTGGAAAGAGTACACAGGTTGGCCGTTAAACGATATGGAGAGATCGTACAAATTCATGGTGAAGCATGTGCAGCTGTGGAAGGTTGCATTTCACAGCACCTCTCCCCGATGGATCCACAGCGCCTATCTCGCCGCCATTGCAGCCTACTATGCCAAGGAGGTGGAGGCTGGTCTAATGGAGTACAAGCCGGACATCATAATCAGTGTGCATCCTTTGATGCAACATATTCCTCTGTGGGTGCTCAAATGGCAAGGCCTGCAGAAGAAGGTGATTTTCGTGACGGTTATCACAGACCTCAACACCTGCCATCCTACATGGTTTCATCCTGGTGTCAATAGGTGCTACAGCCCCTCACAGGAGGTAGCGAAAAGGGCGCTAGTAGATGGCCTTGACGAGTCTCAGATACGGGTCTTTGGCCTGCCCATCCGGCCCTCTTTTGCTCGGGCGGTTTTCTCCAAGGATCAACTCAGAGAAGAACTCGAAATGGACCCTGACTTGCCGGCAGTTCTGCTCATGGGAGGTGGCGAAGGAATGGGGCCTGTAAAGGAAACTGCAAGGGCTCTTGGAGAGACACTCTTTGATAACGAAGCTGGAAAACCAATTGGTCAGCTGATCATCATATGCGGGCGGAATAAAACCCTCGCTGCCACGCTTGAATCCAATGAATGGAACATTCCAGTGAAGGTTAGAGGATTTGAGACACAAATGGAAAAATGGATGGGAGCTTGTGATTGCATCATAACAAAGGCAGGACCTGGCACAATCGCAGAGGCATTGATCAGAGGGCTTCCAATTATCCTCAACGACTACATTCCCGGACAAGAAAAGGGCAATGTGCCTTATGTGGTGGACAATGGGGCCGGCGTCTTCACCAGAAGTCCCAAGGAAACAGCGAGGATTGTCGCAGAATGGTTCAGCACGAAAACAGACGAACTGAAAATGATGTCGGAGAATGCACTAAAACTAGCGCAACCTGAGGCTGTGTTCGACATTGTCAAGGACATCCACGAGCTTGCCCTCCAACGCGGTCCCATGGCGAACATCCCGTACATGCTGACATCTTCGTTTACGAGTTTAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006643 GO:0006664 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008194 GO:0008378 GO:0009267 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009605 GO:0009707 GO:0009941 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016740 GO:0016757 GO:0016758 GO:0019374 GO:0019752 GO:0019867 GO:0031090 GO:0031667 GO:0031668 GO:0031669 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032787 GO:0033554 GO:0035250 GO:0042170 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0098588 GO:0098805 GO:1901135 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko01100 map00561 map01100

Pfam Domains

Protein Families

Glyco_tran_28_C MGDG_synth

Protein Analysis

477

Amino Acids

53.54

Weight (kDa)

6.04

Isoelectric Point (pI)

39.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MGDG_synth	PF06925	90 - 258	1.4e-61	Monogalactosyldiacylglycerol (MGDG) synthase
Glyco_tran_28_C	PF04101	288 - 414	3.9e-10	Glycosyltransferase family 28 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G11810 AT2G11810 AT2G11810 AT2G11810 AT5G20410
fragaria_vesca	FvH4_1g02220 FvH4_1g02220 FvH4_1g02220 FvH4_1g21680
malus_domestica	MD02G1020400.v1.1 MD15G1163200.v1.1
prunus_persica	Prupe.7G251400_v2.0.a1
pyrus_communis	pycom02g01790 pycom15g14680
rosa_chinensis	RchiOBHm_Chr2g0087331 RchiOBHm_Chr3g0481741 RchiOBHm_Chr4g0421821 RchiOBHm_Chr6g0246901
rosa_laevigata	RLG00000003836 RLG00000015857
rosa_multiflora	Rmu_co8187728.1_g000001 Rmu_co8222200.1_g000001 Rmu_sc0000540.1_g000032 Rmu_sc0003367.1_g000024 Rmu_sc0006064.1_g000001 Rmu_sc0007105.1_g000003 Rmu_sc0016592.1_g000001 Rmu_sc0037595.1_g000001
rosa_roxburghii	Rroxscaffold_1G00028410 Rroxscaffold_2G00111080 Rroxscaffold_2G00153680 Rroxscaffold_3G00242830 Rroxscaffold_4G00276430 Rroxscaffold_4G00300210 Rroxscaffold_7G00214190
rosa_rugosa	Rorug01G0474200
rosa_samantha	Rh1CG122800 Rh2AG027800 Rh2AG631400 Rh2BG027300 Rh2BG300600 Rh2CG028100 Rh2DG027900 Rh4BG234200 Rh5CG220000 Rh6BG190700 Rh6CG106700 Rh6CG108200 Rh6DG097200 Rh6DG098600 Rh7CG224800
rosa_wichuraiana	Rw1G013320 Rw2G002200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	659
Acc36I	ACCTGC	1 cut(s)	659
AccB7I	CCANNNNNTGG	1 cut(s)	1014
AccII	CGCG	2 cut(s)	273, 1374
AclWI	GGATC	3 cut(s)	454, 467, 816
AcoI	YGGCCR	2 cut(s)	265, 368
AcsI	RAATTY	1 cut(s)	398
AcyI	GRCGYC	2 cut(s)	14, 1212
AfaI	GTAC	4 cut(s)	359, 395, 530, 1397
AfiI	CCNNNNNNNGG	3 cut(s)	142, 703, 1014
AgsI	TTSAA	1 cut(s)	1004
AjnI	CCWGG	2 cut(s)	673, 1102
AluBI	AGCT	8 cut(s)	81, 202, 218, 421, 938, 958, 1073, 1360
AluI	AGCT	8 cut(s)	81, 202, 218, 421, 938, 958, 1073, 1360
Alw21I	GWGCWC	2 cut(s)	204, 591
Alw26I	GTCTC	4 cut(s)	112, 750, 911, 1040
AlwI	GGATC	3 cut(s)	454, 467, 816
AlwNI	CAGNNNCTG	1 cut(s)	1103
Ama87I	CYCGRG	1 cut(s)	789
AoxI	GGCC	9 cut(s)	265, 285, 368, 601, 733, 763, 776, 884, 1206
ApeKI	GCWGC	6 cut(s)	78, 102, 111, 418, 488, 992
ApoI	RAATTY	1 cut(s)	398
AspLEI	GCGC	4 cut(s)	275, 470, 724, 1318
AspS9I	GGNCC	7 cut(s)	225, 777, 835, 884, 1100, 1206, 1376
AsuC2I	CCSGG	1 cut(s)	1164
AsuHPI	GGTGA	3 cut(s)	418, 628, 1210
AvaI	CYCGRG	1 cut(s)	789
AvaII	GGWCC	4 cut(s)	225, 835, 1100, 1376
AxyI	CCTNAGG	1 cut(s)	1323
BamHI	GGATCC	1 cut(s)	459
BanII	GRGCYC	2 cut(s)	204, 910
BauI	CACGAG	2 cut(s)	203, 1355
BbsI	GAAGAC	1 cut(s)	1207
Bbv12I	GWGCWC	2 cut(s)	204, 591
BbvI	GCAGC	6 cut(s)	90, 114, 123, 430, 500, 979
BccI	CCATC	5 cut(s)	450, 663, 725, 779, 1060
BceAI	ACGGC	2 cut(s)	112, 355
BciT130I	CCWGG	2 cut(s)	675, 1104
BclI	TGATCA	2 cut(s)	960, 1125
BcnI	CCSGG	1 cut(s)	1164
BcoDI	GTCTC	4 cut(s)	112, 750, 911, 1040
BfaI	CTAG	2 cut(s)	725, 1313
BfmI	CTRYAG	2 cut(s)	605, 691
BfoI	RGCGCY	2 cut(s)	471, 725
BfuAI	ACCTGC	1 cut(s)	659
BglI	GCCNNNNNGGC	1 cut(s)	775
Bme1390I	CCNGG	3 cut(s)	675, 1104, 1164
Bme18I	GGWCC	4 cut(s)	225, 835, 1100, 1376
BmeT110I	CYCGRG	1 cut(s)	789
BmgT120I	GGNCC	7 cut(s)	225, 777, 835, 884, 1100, 1206, 1376
BmiI	GGNNCC	5 cut(s)	461, 837, 885, 1207, 1378
BmrFI	CCNGG	3 cut(s)	675, 1104, 1164
BmsI	GCATC	4 cut(s)	286, 555, 565, 1092
BpiI	GAAGAC	1 cut(s)	1207
BplI	GAGNNNNNCTC	4 cut(s)	810, 842, 906, 938
Bpu10I	CCTNAGC	1 cut(s)	74
BpuEI	CTTGAG	1 cut(s)	71
BpuMI	CCSGG	1 cut(s)	1164
BsaHI	GRCGYC	2 cut(s)	14, 1212
BsaJI	CCNNGG	5 cut(s)	282, 501, 804, 1230, 1380
BsaXI	ACNNNNNCTCC	2 cut(s)	377, 407
Bsc4I	CCNNNNNNNGG	3 cut(s)	142, 703, 1014
Bse118I	RCCGGY	3 cut(s)	257, 847, 1208
Bse1I	ACTGG	1 cut(s)	1025
Bse21I	CCTNAGG	1 cut(s)	1323
Bse3DI	GCAATG	2 cut(s)	483, 1186
BseBI	CCWGG	2 cut(s)	675, 1104
BseDI	CCNNGG	5 cut(s)	282, 501, 804, 1230, 1380
BseGI	GGATG	9 cut(s)	190, 349, 556, 655, 670, 771, 1071, 1350, 1389
BseLI	CCNNNNNNNGG	3 cut(s)	142, 703, 1014
BseMI	GCAATG	2 cut(s)	483, 1186
BseMII	CTCAG	5 cut(s)	65, 210, 761, 829, 1314
BseNI	ACTGG	1 cut(s)	1025
BseXI	GCAGC	6 cut(s)	90, 114, 123, 430, 500, 979
BsgI	GTGCAG	2 cut(s)	227, 437
Bsh1236I	CGCG	2 cut(s)	273, 1374
BshFI	GGCC	9 cut(s)	267, 287, 370, 603, 735, 765, 778, 886, 1208
BsiHKAI	GWGCWC	2 cut(s)	204, 591
BsiHKCI	CYCGRG	1 cut(s)	789
BsiSI	CCGG	6 cut(s)	258, 536, 775, 848, 1164, 1209
BslFI	GGGAC	3 cut(s)	205, 1212, 1362
BslI	CCNNNNNNNGG	3 cut(s)	142, 703, 1014
BsmAI	GTCTC	4 cut(s)	112, 750, 911, 1040
BsmFI	GGGAC	3 cut(s)	205, 1212, 1362
BsmI	GAATGC	1 cut(s)	1306
BsnI	GGCC	9 cut(s)	267, 287, 370, 603, 735, 765, 778, 886, 1208
BsoBI	CYCGRG	1 cut(s)	789
Bsp1286I	GDGCHC	3 cut(s)	204, 591, 910
Bsp143I	GATC	5 cut(s)	389, 459, 808, 960, 1125
Bsp19I	CCATGG	1 cut(s)	1380
BspANI	GGCC	9 cut(s)	267, 287, 370, 603, 735, 765, 778, 886, 1208
BspCNI	CTCAG	5 cut(s)	66, 211, 760, 828, 1315
BspFNI	CGCG	2 cut(s)	273, 1374
BspHI	TCATGA	1 cut(s)	246
BspLI	GGNNCC	5 cut(s)	461, 837, 885, 1207, 1378
BspMAI	CTGCAG	1 cut(s)	609
BspMI	ACCTGC	1 cut(s)	659
BspPI	GGATC	3 cut(s)	454, 467, 816
BsrDI	GCAATG	2 cut(s)	483, 1186
BsrFI	RCCGGY	3 cut(s)	257, 847, 1208
BsrI	ACTGG	1 cut(s)	1025
BssAI	RCCGGY	3 cut(s)	257, 847, 1208
BssECI	CCNNGG	5 cut(s)	282, 501, 804, 1230, 1380
BssMI	GATC	5 cut(s)	389, 459, 808, 960, 1125
BssNI	GRCGYC	2 cut(s)	14, 1212
BssSI	CACGAG	2 cut(s)	203, 1355
BssT1I	CCWWGG	4 cut(s)	501, 804, 1230, 1380
Bst2BI	CACGAG	2 cut(s)	203, 1355
Bst2UI	CCWGG	2 cut(s)	675, 1104
Bst4CI	ACNGT	2 cut(s)	196, 631
BstACI	GRCGYC	2 cut(s)	14, 1212
BstAPI	GCANNNNNTGC	1 cut(s)	604
BstC8I	GCNNGC	7 cut(s)	148, 605, 767, 849, 974, 1210, 1362
BstDEI	CTNAG	5 cut(s)	74, 219, 747, 815, 1323
BstDSI	CCRYGG	1 cut(s)	1380
BstF5I	GGATG	9 cut(s)	190, 349, 556, 655, 670, 771, 1071, 1350, 1389
BstFNI	CGCG	2 cut(s)	273, 1374
BstH2I	RGCGCY	2 cut(s)	471, 725
BstHHI	GCGC	4 cut(s)	275, 470, 724, 1318
BstKTI	GATC	5 cut(s)	392, 462, 811, 963, 1128
BstMAI	GTCTC	4 cut(s)	112, 750, 911, 1040
BstMBI	GATC	5 cut(s)	389, 459, 808, 960, 1125
BstNI	CCWGG	2 cut(s)	675, 1104
BstNSI	RCATGY	2 cut(s)	416, 1402
BstSCI	CCNGG	3 cut(s)	673, 1102, 1162
BstSFI	CTRYAG	2 cut(s)	605, 691
BstUI	CGCG	2 cut(s)	273, 1374
BstV1I	GCAGC	6 cut(s)	90, 114, 123, 430, 500, 979
BstV2I	GAAGAC	1 cut(s)	1207
BstX2I	RGATCY	1 cut(s)	459
BstXI	CCANNNNNNTGG	1 cut(s)	508
BstYI	RGATCY	1 cut(s)	459
Bsu36I	CCTNAGG	1 cut(s)	1323
BsuRI	GGCC	9 cut(s)	267, 287, 370, 603, 735, 765, 778, 886, 1208
BtgI	CCRYGG	1 cut(s)	1380
BtgZI	GCGATG	1 cut(s)	333
BtsCI	GGATG	9 cut(s)	190, 349, 556, 655, 670, 771, 1071, 1350, 1389
BtsIMutI	CAGTG	2 cut(s)	556, 1032
BveI	ACCTGC	1 cut(s)	659
Cac8I	GCNNGC	7 cut(s)	148, 605, 767, 849, 974, 1210, 1362
CaiI	CAGNNNCTG	1 cut(s)	1103
CciI	TCATGA	1 cut(s)	246
CfoI	GCGC	4 cut(s)	275, 470, 724, 1318
Cfr10I	RCCGGY	3 cut(s)	257, 847, 1208
Cfr13I	GGNCC	7 cut(s)	225, 777, 835, 884, 1100, 1206, 1376
CseI	GACGC	2 cut(s)	3, 1201
Csp6I	GTAC	4 cut(s)	358, 394, 529, 1396
CviAII	CATG	8 cut(s)	247, 307, 403, 413, 663, 862, 1381, 1399
CviQI	GTAC	4 cut(s)	358, 394, 529, 1396
DdeI	CTNAG	5 cut(s)	74, 219, 747, 815, 1323
DpnI	GATC	5 cut(s)	391, 461, 810, 962, 1127
DpnII	GATC	5 cut(s)	389, 459, 808, 960, 1125
EaeI	YGGCCR	2 cut(s)	265, 368
EciI	GGCGGA	1 cut(s)	991
Ecl136II	GAGCTC	1 cut(s)	202
Eco130I	CCWWGG	4 cut(s)	501, 804, 1230, 1380
Eco147I	AGGCCT	1 cut(s)	603
Eco24I	GRGCYC	2 cut(s)	204, 910
Eco47I	GGWCC	4 cut(s)	225, 835, 1100, 1376
Eco53kI	GAGCTC	1 cut(s)	202
Eco81I	CCTNAGG	1 cut(s)	1323
Eco88I	CYCGRG	1 cut(s)	789
EcoICRI	GAGCTC	1 cut(s)	202
EcoO109I	RGGNCCY	2 cut(s)	884, 1100
EcoRII	CCWGG	2 cut(s)	673, 1102
EcoT14I	CCWWGG	4 cut(s)	501, 804, 1230, 1380
EcoT38I	GRGCYC	2 cut(s)	204, 910
ErhI	CCWWGG	4 cut(s)	501, 804, 1230, 1380
FaeI	CATG	8 cut(s)	250, 310, 406, 416, 666, 865, 1384, 1402
FalI	AAGNNNNNCTT	2 cut(s)	221, 253
FaqI	GGGAC	3 cut(s)	205, 1212, 1362
FatI	CATG	8 cut(s)	246, 306, 402, 412, 662, 861, 1380, 1398
FauI	CCCGC	2 cut(s)	53, 965
FauNDI	CATATG	1 cut(s)	968
FbaI	TGATCA	2 cut(s)	960, 1125
FokI	GGATG	9 cut(s)	197, 356, 543, 642, 657, 758, 1078, 1337, 1376
FriOI	GRGCYC	2 cut(s)	204, 910
FspBI	CTAG	2 cut(s)	725, 1313
GlaI	GCGC	4 cut(s)	274, 469, 723, 1317
HaeII	RGCGCY	2 cut(s)	471, 725
HaeIII	GGCC	9 cut(s)	267, 287, 370, 603, 735, 765, 778, 886, 1208
HapII	CCGG	6 cut(s)	258, 536, 775, 848, 1164, 1209
HgaI	GACGC	2 cut(s)	3, 1201
HhaI	GCGC	4 cut(s)	275, 470, 724, 1318
Hin1I	GRCGYC	2 cut(s)	14, 1212
Hin1II	CATG	8 cut(s)	250, 310, 406, 416, 666, 865, 1384, 1402
Hin6I	GCGC	4 cut(s)	273, 468, 722, 1316
HinP1I	GCGC	4 cut(s)	273, 468, 722, 1316
HinfI	GANTC	3 cut(s)	241, 743, 1004
HpaII	CCGG	6 cut(s)	258, 536, 775, 848, 1164, 1209
HphI	GGTGA	3 cut(s)	418, 628, 1210
Hpy166II	GTNNAC	3 cut(s)	360, 1198, 1416
Hpy188I	TCNGA	6 cut(s)	283, 750, 818, 1130, 1297, 1430
Hpy188III	TCNNGA	5 cut(s)	88, 238, 247, 293, 625
Hpy8I	GTNNAC	3 cut(s)	360, 1198, 1416
Hpy99I	CGWCG	2 cut(s)	19, 161
HpyAV	CCTTC	7 cut(s)	34, 310, 334, 421, 607, 869, 1024
HpyCH4III	ACNGT	2 cut(s)	196, 631
HpyCH4IV	ACGT	1 cut(s)	234
HpyF3I	CTNAG	5 cut(s)	74, 219, 747, 815, 1323
HpySE526I	ACGT	1 cut(s)	234
Hsp92I	GRCGYC	2 cut(s)	14, 1212
Hsp92II	CATG	8 cut(s)	250, 310, 406, 416, 666, 865, 1384, 1402
HspAI	GCGC	4 cut(s)	273, 468, 722, 1316
KroI	GCCGGC	2 cut(s)	847, 1208
KroNI	GCCGGC	2 cut(s)	849, 1210
Ksp22I	TGATCA	2 cut(s)	960, 1125
Kzo9I	GATC	5 cut(s)	389, 459, 808, 960, 1125
LmnI	GCTCC	3 cut(s)	199, 215, 1070
Lsp1109I	GCAGC	6 cut(s)	90, 114, 123, 430, 500, 979
LweI	GCATC	4 cut(s)	286, 555, 565, 1092
MaeI	CTAG	2 cut(s)	725, 1313
MaeII	ACGT	1 cut(s)	234
MaeIII	GTNAC	1 cut(s)	625
MalI	GATC	5 cut(s)	391, 461, 810, 962, 1127
MboI	GATC	5 cut(s)	389, 459, 808, 960, 1125
MboII	GAAGA	4 cut(s)	622, 833, 1207, 1401
MfeI	CAATTG	1 cut(s)	948
MflI	RGATCY	1 cut(s)	459
MhlI	GDGCHC	3 cut(s)	204, 591, 910
MluCI	AATT	3 cut(s)	398, 948, 1140
MlyI	GAGTC	1 cut(s)	752
MmeI	TCCRAC	2 cut(s)	1275, 1393
MroNI	GCCGGC	2 cut(s)	847, 1208
MseI	TTAA	2 cut(s)	374, 1424
MslI	CAYNNNNRTG	2 cut(s)	675, 1025
MspA1I	CMGCKG	2 cut(s)	421, 958
MspI	CCGG	6 cut(s)	258, 536, 775, 848, 1164, 1209
MspR9I	CCNGG	3 cut(s)	675, 1104, 1164
MunI	CAATTG	1 cut(s)	948
Mva1269I	GAATGC	1 cut(s)	1306
MvaI	CCWGG	2 cut(s)	675, 1104
MvnI	CGCG	2 cut(s)	273, 1374
NaeI	GCCGGC	2 cut(s)	849, 1210
NciI	CCSGG	1 cut(s)	1164
NcoI	CCATGG	1 cut(s)	1380
NdeI	CATATG	1 cut(s)	968
NdeII	GATC	5 cut(s)	389, 459, 808, 960, 1125
NgoMIV	GCCGGC	2 cut(s)	847, 1208
NlaIII	CATG	8 cut(s)	250, 310, 406, 416, 666, 865, 1384, 1402
NlaIV	GGNNCC	5 cut(s)	461, 837, 885, 1207, 1378
NmeAIII	GCCGAG	1 cut(s)	45
NmuCI	GTSAC	1 cut(s)	625
NspI	RCATGY	2 cut(s)	416, 1402
PagI	TCATGA	1 cut(s)	246
PaqCI	CACCTGC	1 cut(s)	659
PceI	AGGCCT	1 cut(s)	603
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PctI	GAATGC	1 cut(s)	1306
PdiI	GCCGGC	2 cut(s)	849, 1210
PfeI	GAWTC	2 cut(s)	241, 1004
PflFI	GACNNNGTC	1 cut(s)	1340
PflMI	CCANNNNNTGG	1 cut(s)	1014
PfoI	TCCNGGA	1 cut(s)	1162
PleI	GAGTC	1 cut(s)	751
PpsI	GAGTC	1 cut(s)	751
PpuMI	RGGWCCY	1 cut(s)	1100
Psp124BI	GAGCTC	1 cut(s)	204
Psp5II	RGGWCCY	1 cut(s)	1100
Psp6I	CCWGG	2 cut(s)	673, 1102
PspGI	CCWGG	2 cut(s)	673, 1102
PspN4I	GGNNCC	5 cut(s)	461, 837, 885, 1207, 1378
PspPI	GGNCC	7 cut(s)	225, 777, 835, 884, 1100, 1206, 1376
PspPPI	RGGWCCY	1 cut(s)	1100
PsrI	GAACNNNNNNTAC	2 cut(s)	1379, 1411
PstI	CTGCAG	1 cut(s)	609
PstNI	CAGNNNCTG	1 cut(s)	1103
PsuI	RGATCY	1 cut(s)	459
PsyI	GACNNNGTC	1 cut(s)	1340
PvuII	CAGCTG	2 cut(s)	421, 958
RsaI	GTAC	4 cut(s)	359, 395, 530, 1397
RsaNI	GTAC	4 cut(s)	358, 394, 529, 1396
RseI	CAYNNNNRTG	2 cut(s)	675, 1025
SacI	GAGCTC	1 cut(s)	204
SaqAI	TTAA	2 cut(s)	374, 1424
Sau3AI	GATC	5 cut(s)	389, 459, 808, 960, 1125
Sau96I	GGNCC	7 cut(s)	225, 777, 835, 884, 1100, 1206, 1376
SchI	GAGTC	1 cut(s)	752
ScrFI	CCNGG	3 cut(s)	675, 1104, 1164
SduI	GDGCHC	3 cut(s)	204, 591, 910
SfaNI	GCATC	4 cut(s)	286, 555, 565, 1092
SfcI	CTRYAG	2 cut(s)	605, 691
SgrAI	CRCCGGYG	1 cut(s)	257
SinI	GGWCC	4 cut(s)	225, 835, 1100, 1376
SmiMI	CAYNNNNRTG	2 cut(s)	675, 1025
SmlI	CTYRAG	1 cut(s)	86
SmoI	CTYRAG	1 cut(s)	86
Sse9I	AATT	3 cut(s)	398, 948, 1140
SseBI	AGGCCT	1 cut(s)	603
SspMI	CTAG	2 cut(s)	725, 1313
SstI	GAGCTC	1 cut(s)	204
StuI	AGGCCT	1 cut(s)	603
StyD4I	CCNGG	3 cut(s)	673, 1102, 1162
StyI	CCWWGG	4 cut(s)	501, 804, 1230, 1380
TaaI	ACNGT	2 cut(s)	196, 631
TaiI	ACGT	1 cut(s)	237
TaqI	TCGA	2 cut(s)	828, 1335
TasI	AATT	3 cut(s)	398, 948, 1140
TatI	WGTACW	2 cut(s)	357, 528
TauI	GCSGC	4 cut(s)	102, 264, 267, 482
TfiI	GAWTC	2 cut(s)	241, 1004
Tru1I	TTAA	2 cut(s)	374, 1424
Tru9I	TTAA	2 cut(s)	374, 1424
TscAI	CASTG	2 cut(s)	556, 1032
TseFI	GTSAC	1 cut(s)	625
TseI	GCWGC	6 cut(s)	78, 102, 111, 418, 488, 992
Tsp45I	GTSAC	1 cut(s)	625
TspDTI	ATGAA	4 cut(s)	336, 391, 659, 1026
TspRI	CASTG	2 cut(s)	556, 1032
Tth111I	GACNNNGTC	1 cut(s)	1340
Van91I	CCANNNNNTGG	1 cut(s)	1014
VpaK11BI	GGWCC	4 cut(s)	225, 835, 1100, 1376
XapI	RAATTY	1 cut(s)	398
XceI	RCATGY	2 cut(s)	416, 1402
XspI	CTAG	2 cut(s)	725, 1313

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.