Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001173

Physical Location & Seq

Genomic Coordinates Forward (+)

13243010 .. 13247140

Gene Structure 4131 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1162200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGGCGGCGGGATCAGTGGCGACCGCCGCCGGAGTCGCCGTGATTCTTTACTACGTGTTCAATCGGAGGGGAGCAACGAAAGAGGGCGGCGTTGAAGACGGGGACGACGACGGCGATCGGAGCGGAAATCTCTCGAAGATGAGATCGGCGAGGAAACGGCTTTCTCGGCGACCGGCTCAAGCGCCCGCCACGTGGGTCGAGTCGCTCACCACCTTGTCCGACACGCTCCGGTTCACCTACTCCGAAACGCTCGGGAAGTGGCCGATCGGCGACTTGGCCTTCGGCATTAAGTACCTCATGCGTCGGCAGGGTAACCTAAAAGTTGCGAGTGTGTATGCTGGTAGTGATAGTGTGCAGCTTAAAGGGTCTGGAATTATTGAGGAGTTGAAATATTACTTGAAGTTGCTGACGCTTTGTATGTTGTTCTCGAAGAAACCGTTTCCGGTGTTTTTAGAGTCAGGGGGTTTCTCTCAGGAAGATGTCCTTCTTCAGAAGCCCAAGGCCGGGCTTCTGAAGCCATCCTTCACAATTATACGTGATAGGAATTCAAAATGCTTCCTTCTATTAATACGTGGTACCCATAGCATCAAAGATACACTAACTGCTGCAACTGGTGCAGTAGCCCCTTTCCACCATTCGGTTTTGCATGATGGTGGAATAAGCAACTTAATCCTAGGGTATGCTCACTGTGGGATGGTTGCTGCAGCCCGTTGGATTGCTAAGATCAGTACTCCTTGCTTGCTTAAGGCTATTGGTGAATATCCTGACTACAAAATAAAGGTTGTTGGGCATTCACTGGGTGGTGGTACAGCTGCGTTGTTAACATATATTCTTCGAGAACGGAAAGAATTCTCTTCAAGCAATTGCATTACCTTTGCCCCAGCTGCCTGTATAACATGGGAGTTGGCAGAATCTGGAAAGCACTTCATCACTACTATAATCAATGGCTCTGACCTGGTGCCTACTTTCTCAGCAGCTTCTGTTGATGACCTCCGCTCTGAGGTTATAGCTTCATCTTGGTTAAATGATTTGCGGGATCAAGTTGAGCGCACACGAGTTTTGAATGTTGTTTATCGCTCTGCAAATGCTCTGGGTTCTCGTCTACCATCTATAGCTAGTGCAAAAGCGAGGGTTGCCGGTGCAGGTGCACTATTGCGGCCAGTATCCAGCAGCACGCAGATTGTGATGAAGCGTGCACAAAATGTTGTTGTGAGAACCCATTCATCAATCTCATCGTGGTCTTGCATGGGTGCACGTCGACGCAATGTAAGCCCATTACTGAACTCTAAAGCAGACGATTTGCATGAAGATTCTCTCATATGTGAAAAGGATTCTGAATATCGCGCTGAAGGAGTAATTGTGGACCCAATGCTGAATAACTTGGAATCTAGTTCTAGCGGTGGATTGGGTCATGATGACAGCGATGAAGAGGAGCAGCTTTTGCCAGTGAATAGAAATACTGCAACATCTACCGTTGAAGACATTACTGAAGGCGAGTTGTGGTATGAATTGGAGAAGGAGCTCAAGAGGCAGGAGGATGAAGTCAATGTCGAGGCCCGAGAGGAAGAGGCTGCTGCAGTGAAAGAAATAACCGAGGAAGAAGATATGCTGGTTGGTGTTGCAGAAAGCAATACGCCAATCTCTTCCTCGGATGTTTCAGAGAACCACCGCTTCTTTCCCCCTGGCAGAATCATGCACATCATATCAGTCCCTTCATCTGATGATACAAATTTAGATAACGACGGACCACCAGCCGAAGAACGCGTGGGCATATACGAGACACGTAGACAACTGTACAGTAAGCTCCGACTTTCTAAAACGATGATTAATGATCACTACATGCCTATGTATAAGAAGATGGTGGAATTGTTGATCAGGGAACTGGAAAATGACGAATCCAGTAATTGTATCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006638 GO:0006639 GO:0006690 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019752 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:1901568 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase3_N Lipase_3

Protein Analysis

639

Amino Acids

70.09

Weight (kDa)

5.83

Isoelectric Point (pI)

51.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase3_N	PF03893	53 - 133	4.6e-15	Lipase 3 N-terminal region
Lipase_3	PF01764	188 - 325	7.1e-28	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16070 AT4G16070 AT4G16070
fragaria_vesca	FvH4_4g18880
malus_domestica	MD13G1162400.v1.1 MD16G1162200.v1.1
prunus_persica	Prupe.1G129700_v2.0.a1
pyrus_communis	pycom13g13870 pycom16g13760
rosa_chinensis	RchiOBHm_Chr2g0160841 RchiOBHm_Chr4g0423781 RchiOBHm_Chr4g0423981
rosa_laevigata	RLG00000000522 RLG00000007533 RLG00000013372 RLG00000014048 RLG00000023214
rosa_multiflora	Rmu_co8377889.1_g000001 Rmu_sc0000045.1_g000074 Rmu_sc0000389.1_g000004 Rmu_sc0000464.1_g000001 Rmu_sc0001389.1_g000003 Rmu_sc0003002.1_g000068 Rmu_sc0007269.1_g000005 Rmu_sc0007293.1_g000006 Rmu_sc0016022.1_g000001 Rmu_sc0016156.1_g000001 Rmu_sc0027619.1_g000001 Rmu_ssc0000400.1_g000003
rosa_roxburghii	Rroxscaffold_4G00314330 Rroxscaffold_5G00346900 Rroxscaffold_5G00365890 Rroxscaffold_7G00210200
rosa_rugosa	Rorug01G0273200 Rorug02G0105800 Rorug02G0297900.1 Rorug04G0190400
rosa_samantha	Rh1AG191500 Rh1BG000800 Rh1BG300500 Rh1DG332300 Rh2BG210100 Rh2BG565600 Rh2BG565700 Rh2CG202400 Rh4AG250000 Rh4BG255200 Rh4CG263200 Rh4DG249500 Rh5BG272200 Rh5DG149700 Rh5DG277000 Rh5DG280100 Rh6CG191500
rosa_wichuraiana	Rw1G017810 Rw4G021400 Rw7G017410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1133
Acc36I	ACCTGC	1 cut(s)	1133
Acc65I	GGTACC	1 cut(s)	575
AccB1I	GGYRCC	2 cut(s)	575, 958
AccBSI	CCGCTC	2 cut(s)	123, 996
AccI	GTMKAC	3 cut(s)	1102, 1258, 1786
AccII	CGCG	2 cut(s)	1344, 1764
AclWI	GGATC	2 cut(s)	19, 1044
AcoI	YGGCCR	2 cut(s)	260, 1157
AcsI	RAATTY	3 cut(s)	544, 848, 1729
AcuI	CTGAAG	4 cut(s)	473, 533, 1368, 1509
AcvI	CACGTG	1 cut(s)	192
AdeI	CACNNNGTG	1 cut(s)	800
AfaI	GTAC	5 cut(s)	293, 577, 730, 808, 1796
AfiI	CCNNNNNNNGG	5 cut(s)	192, 503, 504, 637, 1000
AflII	CTTAAG	1 cut(s)	743
AflIII	ACRYGT	3 cut(s)	54, 1762, 1781
AgsI	TTSAA	8 cut(s)	61, 95, 388, 400, 549, 858, 1063, 1478
AjiI	CACGTC	1 cut(s)	1256
AjnI	CCWGG	2 cut(s)	954, 1681
AluBI	AGCT	9 cut(s)	358, 812, 884, 977, 1010, 1115, 1438, 1522, 1804
AluI	AGCT	9 cut(s)	358, 812, 884, 977, 1010, 1115, 1438, 1522, 1804
Alw21I	GWGCWC	4 cut(s)	1150, 1198, 1255, 1524
Alw26I	GTCTC	1 cut(s)	1772
Alw44I	GTGCAC	3 cut(s)	1146, 1194, 1251
AlwI	GGATC	2 cut(s)	19, 1044
AlwNI	CAGNNNCTG	2 cut(s)	914, 980
Ama87I	CYCGRG	2 cut(s)	251, 1557
AoxI	GGCC	5 cut(s)	260, 276, 501, 1157, 1554
ApaLI	GTGCAC	3 cut(s)	1146, 1194, 1251
ApoI	RAATTY	3 cut(s)	544, 848, 1729
ArsI	GACNNNNNNTTYG	2 cut(s)	263, 295
AseI	ATTAAT	2 cut(s)	566, 1827
Asp700I	GAANNNNTTC	2 cut(s)	848, 1219
Asp718I	GGTACC	1 cut(s)	575
AspA2I	CCTAGG	1 cut(s)	673
AspLEI	GCGC	3 cut(s)	184, 1050, 1346
AspS9I	GGNCC	3 cut(s)	1363, 1555, 1745
AsuC2I	CCSGG	1 cut(s)	505
AsuHPI	GGTGA	3 cut(s)	199, 226, 767
AvaI	CYCGRG	2 cut(s)	251, 1557
AvaII	GGWCC	2 cut(s)	1363, 1745
AvrII	CCTAGG	1 cut(s)	673
BaeGI	GKGCMC	3 cut(s)	1150, 1198, 1255
BanI	GGYRCC	2 cut(s)	575, 958
BanII	GRGCYC	1 cut(s)	1524
BauI	CACGAG	1 cut(s)	1053
BbrPI	CACGTG	1 cut(s)	192
BbsI	GAAGAC	2 cut(s)	102, 1485
Bbv12I	GWGCWC	4 cut(s)	1150, 1198, 1255, 1524
BccI	CCATC	5 cut(s)	526, 644, 688, 1114, 1852
BceAI	ACGGC	3 cut(s)	23, 127, 173
BciT130I	CCWGG	2 cut(s)	956, 1683
BciVI	GTATCC	1 cut(s)	1174
BclI	TGATCA	2 cut(s)	1831, 1872
BcnI	CCSGG	1 cut(s)	505
BcoDI	GTCTC	1 cut(s)	1772
BfaI	CTAG	4 cut(s)	674, 1116, 1389, 1395
BfmI	CTRYAG	3 cut(s)	702, 1110, 1575
BfoI	RGCGCY	1 cut(s)	185
BfrI	CTTAAG	1 cut(s)	743
BfuAI	ACCTGC	1 cut(s)	1133
BfuI	GTATCC	1 cut(s)	1174
BlnI	CCTAGG	1 cut(s)	673
BmcAI	AGTACT	1 cut(s)	730
Bme1390I	CCNGG	3 cut(s)	505, 956, 1683
Bme18I	GGWCC	2 cut(s)	1363, 1745
BmeT110I	CYCGRG	2 cut(s)	251, 1557
BmgBI	CACGTC	1 cut(s)	1256
BmgT120I	GGNCC	3 cut(s)	1363, 1555, 1745
BmiI	GGNNCC	3 cut(s)	577, 960, 1365
BmrFI	CCNGG	3 cut(s)	505, 956, 1683
BmrI	ACTGGG	1 cut(s)	806
BmsI	GCATC	1 cut(s)	594
BmuI	ACTGGG	1 cut(s)	806
BpiI	GAAGAC	2 cut(s)	102, 1485
BpuEI	CTTGAG	2 cut(s)	162, 1508
BpuMI	CCSGG	1 cut(s)	505
BsaAI	YACGTR	5 cut(s)	55, 192, 536, 572, 1784
BsaJI	CCNNGG	5 cut(s)	498, 673, 1593, 1647, 1681
BsaWI	WCCGGW	2 cut(s)	228, 442
BsaXI	ACNNNNNCTCC	4 cut(s)	24, 54, 1344, 1374
Bsc4I	CCNNNNNNNGG	5 cut(s)	192, 503, 504, 637, 1000
Bse118I	RCCGGY	2 cut(s)	172, 1136
Bse1I	ACTGG	6 cut(s)	616, 801, 1160, 1445, 1887, 1899
Bse3DI	GCAATG	1 cut(s)	1270
BseBI	CCWGG	2 cut(s)	956, 1683
BseDI	CCNNGG	5 cut(s)	498, 673, 1593, 1647, 1681
BseGI	GGATG	4 cut(s)	518, 699, 1543, 1657
BseLI	CCNNNNNNNGG	5 cut(s)	192, 503, 504, 637, 1000
BseMI	GCAATG	1 cut(s)	1270
BseMII	CTCAG	3 cut(s)	485, 984, 990
BseNI	ACTGG	6 cut(s)	616, 801, 1160, 1445, 1887, 1899
BseRI	GAGGAG	2 cut(s)	395, 1445
BseSI	GKGCMC	3 cut(s)	1150, 1198, 1255
BseYI	CCCAGC	1 cut(s)	880
BsgI	GTGCAG	3 cut(s)	374, 636, 1161
Bsh1236I	CGCG	2 cut(s)	1344, 1764
Bsh1285I	CGRYCG	4 cut(s)	24, 118, 173, 267
BshFI	GGCC	5 cut(s)	262, 278, 503, 1159, 1556
BshNI	GGYRCC	2 cut(s)	575, 958
BsiEI	CGRYCG	4 cut(s)	24, 118, 173, 267
BsiHKAI	GWGCWC	4 cut(s)	1150, 1198, 1255, 1524
BsiHKCI	CYCGRG	2 cut(s)	251, 1557
BsiSI	CCGG	6 cut(s)	30, 173, 229, 443, 504, 1137
BslFI	GGGAC	2 cut(s)	116, 1694
BslI	CCNNNNNNNGG	5 cut(s)	192, 503, 504, 637, 1000
BsmAI	GTCTC	1 cut(s)	1772
BsmFI	GGGAC	2 cut(s)	116, 1694
BsmI	GAATGC	1 cut(s)	790
BsnI	GGCC	5 cut(s)	262, 278, 503, 1159, 1556
BsoBI	CYCGRG	2 cut(s)	251, 1557
Bsp1286I	GDGCHC	4 cut(s)	1150, 1198, 1255, 1524
Bsp1407I	TGTACA	1 cut(s)	1794
Bsp143I	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
BspANI	GGCC	5 cut(s)	262, 278, 503, 1159, 1556
BspCNI	CTCAG	3 cut(s)	484, 983, 991
BspFNI	CGCG	2 cut(s)	1344, 1764
BspHI	TCATGA	1 cut(s)	1411
BspLI	GGNNCC	3 cut(s)	577, 960, 1365
BspMAI	CTGCAG	2 cut(s)	706, 1579
BspMI	ACCTGC	1 cut(s)	1133
BspPI	GGATC	2 cut(s)	19, 1044
BspT107I	GGYRCC	2 cut(s)	575, 958
BspTI	CTTAAG	1 cut(s)	743
BsrBI	CCGCTC	2 cut(s)	123, 996
BsrDI	GCAATG	1 cut(s)	1270
BsrFI	RCCGGY	2 cut(s)	172, 1136
BsrGI	TGTACA	1 cut(s)	1794
BsrI	ACTGG	6 cut(s)	616, 801, 1160, 1445, 1887, 1899
BssAI	RCCGGY	2 cut(s)	172, 1136
BssECI	CCNNGG	5 cut(s)	498, 673, 1593, 1647, 1681
BssMI	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
BssSI	CACGAG	1 cut(s)	1053
BssT1I	CCWWGG	2 cut(s)	498, 673
Bst2BI	CACGAG	1 cut(s)	1053
Bst2UI	CCWGG	2 cut(s)	956, 1683
Bst4CI	ACNGT	5 cut(s)	438, 689, 1474, 1794, 1799
Bst6I	CTCTTC	4 cut(s)	859, 1422, 1560, 1648
BstAFI	CTTAAG	1 cut(s)	743
BstAPI	GCANNNNNTGC	2 cut(s)	614, 1441
BstAUI	TGTACA	1 cut(s)	1794
BstBAI	YACGTR	5 cut(s)	55, 192, 536, 572, 1784
BstC8I	GCNNGC	4 cut(s)	186, 740, 1175, 1194
BstDEI	CTNAG	4 cut(s)	471, 720, 970, 999
BstEII	GGTNACC	1 cut(s)	311
BstF5I	GGATG	4 cut(s)	518, 699, 1543, 1657
BstFNI	CGCG	2 cut(s)	1344, 1764
BstH2I	RGCGCY	1 cut(s)	185
BstHHI	GCGC	3 cut(s)	184, 1050, 1346
BstKTI	GATC	8 cut(s)	14, 118, 146, 267, 726, 1039, 1834, 1875
BstMAI	GTCTC	1 cut(s)	1772
BstMBI	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
BstMCI	CGRYCG	4 cut(s)	24, 118, 173, 267
BstNI	CCWGG	2 cut(s)	956, 1683
BstNSI	RCATGY	1 cut(s)	1843
BstPI	GGTNACC	1 cut(s)	311
BstSCI	CCNGG	3 cut(s)	503, 954, 1681
BstSFI	CTRYAG	3 cut(s)	702, 1110, 1575
BstSLI	GKGCMC	3 cut(s)	1150, 1198, 1255
BstUI	CGCG	2 cut(s)	1344, 1764
BstV2I	GAAGAC	2 cut(s)	102, 1485
BsuI	GTATCC	1 cut(s)	1174
BsuRI	GGCC	5 cut(s)	262, 278, 503, 1159, 1556
BtgZI	GCGATG	1 cut(s)	1437
BtrI	CACGTC	1 cut(s)	1256
BtsCI	GGATG	4 cut(s)	518, 699, 1543, 1657
BtsI	GCAGTG	1 cut(s)	1584
BtsIMutI	CAGTG	5 cut(s)	21, 685, 794, 1452, 1584
BveI	ACCTGC	1 cut(s)	1133
Cac8I	GCNNGC	4 cut(s)	186, 740, 1175, 1194
CaiI	CAGNNNCTG	2 cut(s)	914, 980
CciI	TCATGA	1 cut(s)	1411
CfoI	GCGC	3 cut(s)	184, 1050, 1346
Cfr10I	RCCGGY	2 cut(s)	172, 1136
Cfr13I	GGNCC	3 cut(s)	1363, 1555, 1745
CseI	GACGC	3 cut(s)	290, 418, 1269
CsiI	ACCWGGT	1 cut(s)	954
Csp6I	GTAC	5 cut(s)	292, 576, 729, 807, 1795
CviAII	CATG	8 cut(s)	298, 647, 897, 1246, 1304, 1412, 1693, 1840
CviQI	GTAC	5 cut(s)	292, 576, 729, 807, 1795
DdeI	CTNAG	4 cut(s)	471, 720, 970, 999
DpnI	GATC	8 cut(s)	13, 117, 145, 266, 725, 1038, 1833, 1874
DpnII	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
DraIII	CACNNNGTG	1 cut(s)	800
EaeI	YGGCCR	2 cut(s)	260, 1157
Eam1104I	CTCTTC	4 cut(s)	859, 1422, 1560, 1648
EarI	CTCTTC	4 cut(s)	859, 1422, 1560, 1648
Ecl136II	GAGCTC	1 cut(s)	1522
Eco130I	CCWWGG	2 cut(s)	498, 673
Eco24I	GRGCYC	1 cut(s)	1524
Eco47I	GGWCC	2 cut(s)	1363, 1745
Eco53kI	GAGCTC	1 cut(s)	1522
Eco57I	CTGAAG	4 cut(s)	473, 533, 1368, 1509
Eco72I	CACGTG	1 cut(s)	192
Eco88I	CYCGRG	2 cut(s)	251, 1557
Eco91I	GGTNACC	1 cut(s)	311
EcoICRI	GAGCTC	1 cut(s)	1522
EcoO65I	GGTNACC	1 cut(s)	311
EcoRI	GAATTC	2 cut(s)	544, 848
EcoRII	CCWGG	2 cut(s)	954, 1681
EcoT14I	CCWWGG	2 cut(s)	498, 673
EcoT38I	GRGCYC	1 cut(s)	1524
ErhI	CCWWGG	2 cut(s)	498, 673
FaeI	CATG	8 cut(s)	301, 650, 900, 1249, 1307, 1415, 1696, 1843
FalI	AAGNNNNNCTT	4 cut(s)	468, 500, 506, 538
FaqI	GGGAC	2 cut(s)	116, 1694
FatI	CATG	8 cut(s)	297, 646, 896, 1245, 1303, 1411, 1692, 1839
FauI	CCCGC	2 cut(s)	193, 1026
FauNDI	CATATG	1 cut(s)	1319
FbaI	TGATCA	2 cut(s)	1831, 1872
FblI	GTMKAC	3 cut(s)	1102, 1258, 1786
FokI	GGATG	4 cut(s)	505, 706, 1550, 1664
FriOI	GRGCYC	1 cut(s)	1524
FspBI	CTAG	4 cut(s)	674, 1116, 1389, 1395
GlaI	GCGC	3 cut(s)	183, 1049, 1345
GsaI	CCCAGC	1 cut(s)	884
HaeII	RGCGCY	1 cut(s)	185
HaeIII	GGCC	5 cut(s)	262, 278, 503, 1159, 1556
HapII	CCGG	6 cut(s)	30, 173, 229, 443, 504, 1137
HgaI	GACGC	3 cut(s)	290, 418, 1269
HhaI	GCGC	3 cut(s)	184, 1050, 1346
Hin1II	CATG	8 cut(s)	301, 650, 900, 1249, 1307, 1415, 1696, 1843
Hin6I	GCGC	3 cut(s)	182, 1048, 1344
HinP1I	GCGC	3 cut(s)	182, 1048, 1344
HincII	GTYRAC	2 cut(s)	822, 1259
HindII	GTYRAC	2 cut(s)	822, 1259
HpaI	GTTAAC	1 cut(s)	822
HpaII	CCGG	6 cut(s)	30, 173, 229, 443, 504, 1137
HphI	GGTGA	3 cut(s)	199, 226, 767
Hpy166II	GTNNAC	9 cut(s)	234, 822, 1103, 1148, 1196, 1253, 1259, 1363, 1787
Hpy8I	GTNNAC	9 cut(s)	234, 822, 1103, 1148, 1196, 1253, 1259, 1363, 1787
Hpy99I	CGWCG	6 cut(s)	110, 113, 306, 1260, 1263, 1745
HpyAV	CCTTC	8 cut(s)	289, 494, 532, 569, 1343, 1484, 1510, 1722
HpyCH4III	ACNGT	5 cut(s)	438, 689, 1474, 1794, 1799
HpyCH4IV	ACGT	6 cut(s)	54, 191, 535, 571, 1255, 1783
HpyF3I	CTNAG	4 cut(s)	471, 720, 970, 999
HpySE526I	ACGT	6 cut(s)	54, 191, 535, 571, 1255, 1783
Hsp92II	CATG	8 cut(s)	301, 650, 900, 1249, 1307, 1415, 1696, 1843
HspAI	GCGC	3 cut(s)	182, 1048, 1344
KpnI	GGTACC	1 cut(s)	579
Ksp22I	TGATCA	2 cut(s)	1831, 1872
KspAI	GTTAAC	1 cut(s)	822
Kzo9I	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
LmnI	GCTCC	6 cut(s)	71, 120, 231, 1432, 1519, 1809
LweI	GCATC	1 cut(s)	594
MabI	ACCWGGT	1 cut(s)	954
MaeI	CTAG	4 cut(s)	674, 1116, 1389, 1395
MaeII	ACGT	6 cut(s)	54, 191, 535, 571, 1255, 1783
MaeIII	GTNAC	1 cut(s)	311
MalI	GATC	8 cut(s)	13, 117, 145, 266, 725, 1038, 1833, 1874
MbiI	CCGCTC	2 cut(s)	123, 996
MboI	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
MfeI	CAATTG	1 cut(s)	862
MhlI	GDGCHC	4 cut(s)	1150, 1198, 1255, 1524
MluI	ACGCGT	1 cut(s)	1762
MlyI	GAGTC	3 cut(s)	42, 209, 464
MmeI	TCCRAC	3 cut(s)	243, 692, 1831
MroXI	GAANNNNTTC	2 cut(s)	848, 1219
MseI	TTAA	8 cut(s)	288, 360, 566, 668, 744, 821, 1022, 1827
MslI	CAYNNNNRTG	2 cut(s)	651, 1844
MspA1I	CMGCKG	2 cut(s)	812, 884
MspCI	CTTAAG	1 cut(s)	743
MspI	CCGG	6 cut(s)	30, 173, 229, 443, 504, 1137
MspR9I	CCNGG	3 cut(s)	505, 956, 1683
MunI	CAATTG	1 cut(s)	862
Mva1269I	GAATGC	1 cut(s)	790
MvaI	CCWGG	2 cut(s)	956, 1683
MvnI	CGCG	2 cut(s)	1344, 1764
NciI	CCSGG	1 cut(s)	505
NdeI	CATATG	1 cut(s)	1319
NdeII	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
NlaIII	CATG	8 cut(s)	301, 650, 900, 1249, 1307, 1415, 1696, 1843
NlaIV	GGNNCC	3 cut(s)	577, 960, 1365
NmeAIII	GCCGAG	1 cut(s)	145
NspI	RCATGY	1 cut(s)	1843
PagI	TCATGA	1 cut(s)	1411
PaqCI	CACCTGC	1 cut(s)	1133
PcsI	WCGNNNNNNNCGW	3 cut(s)	105, 111, 434
PctI	GAATGC	1 cut(s)	790
PdmI	GAANNNNTTC	2 cut(s)	848, 1219
PfeI	GAWTC	7 cut(s)	43, 911, 1310, 1331, 1385, 1689, 1895
Ple19I	CGATCG	2 cut(s)	118, 267
PleI	GAGTC	3 cut(s)	41, 208, 463
PmaCI	CACGTG	1 cut(s)	192
PmlI	CACGTG	1 cut(s)	192
PpsI	GAGTC	3 cut(s)	41, 208, 463
Ppu21I	YACGTR	5 cut(s)	55, 192, 536, 572, 1784
PshBI	ATTAAT	2 cut(s)	566, 1827
Psp124BI	GAGCTC	1 cut(s)	1524
Psp6I	CCWGG	2 cut(s)	954, 1681
PspCI	CACGTG	1 cut(s)	192
PspEI	GGTNACC	1 cut(s)	311
PspFI	CCCAGC	1 cut(s)	880
PspGI	CCWGG	2 cut(s)	954, 1681
PspN4I	GGNNCC	3 cut(s)	577, 960, 1365
PspPI	GGNCC	3 cut(s)	1363, 1555, 1745
PstI	CTGCAG	2 cut(s)	706, 1579
PstNI	CAGNNNCTG	2 cut(s)	914, 980
PvuI	CGATCG	2 cut(s)	118, 267
PvuII	CAGCTG	2 cut(s)	812, 884
RsaI	GTAC	5 cut(s)	293, 577, 730, 808, 1796
RsaNI	GTAC	5 cut(s)	292, 576, 729, 807, 1795
RseI	CAYNNNNRTG	2 cut(s)	651, 1844
SacI	GAGCTC	1 cut(s)	1524
SalI	GTCGAC	1 cut(s)	1257
SaqAI	TTAA	8 cut(s)	288, 360, 566, 668, 744, 821, 1022, 1827
Sau3AI	GATC	8 cut(s)	11, 115, 143, 264, 723, 1036, 1831, 1872
Sau96I	GGNCC	3 cut(s)	1363, 1555, 1745
ScaI	AGTACT	1 cut(s)	730
SchI	GAGTC	3 cut(s)	42, 209, 464
ScrFI	CCNGG	3 cut(s)	505, 956, 1683
SduI	GDGCHC	4 cut(s)	1150, 1198, 1255, 1524
SexAI	ACCWGGT	1 cut(s)	954
SfaNI	GCATC	1 cut(s)	594
SfcI	CTRYAG	3 cut(s)	702, 1110, 1575
SgrDI	CGTCGACG	1 cut(s)	1257
SinI	GGWCC	2 cut(s)	1363, 1745
SmiMI	CAYNNNNRTG	2 cut(s)	651, 1844
SmlI	CTYRAG	3 cut(s)	177, 743, 1523
SmoI	CTYRAG	3 cut(s)	177, 743, 1523
SspI	AATATT	1 cut(s)	392
SspMI	CTAG	4 cut(s)	674, 1116, 1389, 1395
SstI	GAGCTC	1 cut(s)	1524
StyD4I	CCNGG	3 cut(s)	503, 954, 1681
StyI	CCWWGG	2 cut(s)	498, 673
TaaI	ACNGT	5 cut(s)	438, 689, 1474, 1794, 1799
TaiI	ACGT	6 cut(s)	57, 194, 538, 574, 1258, 1786
TaqI	TCGA	6 cut(s)	134, 198, 428, 835, 1258, 1551
TatI	WGTACW	2 cut(s)	728, 1794
TauI	GCSGC	4 cut(s)	8, 29, 90, 1159
TfiI	GAWTC	7 cut(s)	43, 911, 1310, 1331, 1385, 1689, 1895
Tru1I	TTAA	8 cut(s)	288, 360, 566, 668, 744, 821, 1022, 1827
Tru9I	TTAA	8 cut(s)	288, 360, 566, 668, 744, 821, 1022, 1827
TscAI	CASTG	5 cut(s)	21, 692, 801, 1452, 1584
TspDTI	ATGAA	9 cut(s)	916, 1002, 1202, 1212, 1320, 1440, 1521, 1554, 1704
TspGWI	ACGGA	2 cut(s)	856, 1758
TspRI	CASTG	5 cut(s)	21, 692, 801, 1452, 1584
Vha464I	CTTAAG	1 cut(s)	743
VneI	GTGCAC	3 cut(s)	1146, 1194, 1251
VpaK11BI	GGWCC	2 cut(s)	1363, 1745
VspI	ATTAAT	2 cut(s)	566, 1827
XapI	RAATTY	3 cut(s)	544, 848, 1729
XceI	RCATGY	1 cut(s)	1843
XmaJI	CCTAGG	1 cut(s)	673
XmiI	GTMKAC	3 cut(s)	1102, 1258, 1786
XmnI	GAANNNNTTC	2 cut(s)	848, 1219
XspI	CTAG	4 cut(s)	674, 1116, 1389, 1395
ZrmI	AGTACT	1 cut(s)	730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.