Encoded by
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1106 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2621 |
| Acc36I | ACCTGC | 1 cut(s) | 1106 |
| Acc65I | GGTACC | 1 cut(s) | 2377 |
| AccB1I | GGYRCC | 5 cut(s) | 178, 931, 2001, 2377, 2692 |
| AccBSI | CCGCTC | 2 cut(s) | 120, 969 |
| AccI | GTMKAC | 4 cut(s) | 767, 1075, 2397, 2464 |
| AclWI | GGATC | 5 cut(s) | 200, 1017, 1390, 1430, 2172 |
| AcoI | YGGCCR | 4 cut(s) | 61, 183, 257, 1326 |
| AcsI | RAATTY | 3 cut(s) | 310, 1994, 2146 |
| AcuI | CTGAAG | 4 cut(s) | 470, 530, 1488, 2343 |
| AcvI | CACGTG | 2 cut(s) | 189, 1028 |
| AcyI | GRCGYC | 1 cut(s) | 179 |
| AfaI | GTAC | 7 cut(s) | 50, 574, 727, 805, 1147, 2275, 2379 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 634, 973, 1249, 1354 |
| AflII | CTTAAG | 2 cut(s) | 740, 2237 |
| AflIII | ACRYGT | 4 cut(s) | 54, 1025, 1027, 2323 |
| AgeI | ACCGGT | 1 cut(s) | 1647 |
| AgsI | TTSAA | 7 cut(s) | 397, 546, 855, 1036, 1256, 1264, 2404 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2517 |
| AjiI | CACGTC | 1 cut(s) | 1238 |
| AjnI | CCWGG | 5 cut(s) | 927, 1100, 1660, 2229, 2515 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1725, 1757 |
| Alw21I | GWGCWC | 3 cut(s) | 1123, 1171, 1237 |
| Alw26I | GTCTC | 3 cut(s) | 236, 1832, 2848 |
| Alw44I | GTGCAC | 3 cut(s) | 1119, 1167, 1233 |
| AlwI | GGATC | 5 cut(s) | 200, 1017, 1390, 1430, 2172 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 887, 953, 1058 |
| Ama87I | CYCGRG | 1 cut(s) | 2337 |
| AoxI | GGCC | 9 cut(s) | 61, 183, 257, 273, 618, 1130, 1326, 1951, 2349 |
| ApaLI | GTGCAC | 3 cut(s) | 1119, 1167, 1233 |
| ApoI | RAATTY | 3 cut(s) | 310, 1994, 2146 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 260, 292, 2442, 2474 |
| AsiGI | ACCGGT | 1 cut(s) | 1647 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1201 |
| Asp718I | GGTACC | 1 cut(s) | 2377 |
| AspA2I | CCTAGG | 1 cut(s) | 670 |
| AspLEI | GCGC | 3 cut(s) | 181, 365, 505 |
| AspS9I | GGNCC | 4 cut(s) | 619, 1724, 2227, 2442 |
| AsuC2I | CCSGG | 2 cut(s) | 833, 2478 |
| AsuHPI | GGTGA | 9 cut(s) | 514, 764, 1289, 1484, 1835, 2315, 2342, 2555, 2798 |
| AvaI | CYCGRG | 1 cut(s) | 2337 |
| AvaII | GGWCC | 3 cut(s) | 1724, 2227, 2442 |
| AvrII | CCTAGG | 1 cut(s) | 670 |
| AxyI | CCTNAGG | 1 cut(s) | 2418 |
| BaeGI | GKGCMC | 3 cut(s) | 1123, 1171, 1237 |
| BanI | GGYRCC | 5 cut(s) | 178, 931, 2001, 2377, 2692 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2260, 2292 |
| BbrPI | CACGTG | 2 cut(s) | 189, 1028 |
| BbsI | GAAGAC | 4 cut(s) | 1464, 1811, 2016, 2870 |
| Bbv12I | GWGCWC | 3 cut(s) | 1123, 1171, 1237 |
| BceAI | ACGGC | 3 cut(s) | 100, 418, 1227 |
| BciT130I | CCWGG | 5 cut(s) | 929, 1102, 1662, 2231, 2517 |
| BciVI | GTATCC | 1 cut(s) | 1147 |
| BcnI | CCSGG | 2 cut(s) | 833, 2478 |
| BcoDI | GTCTC | 3 cut(s) | 236, 1832, 2848 |
| BfaI | CTAG | 6 cut(s) | 147, 248, 671, 1089, 1184, 2630 |
| BfmI | CTRYAG | 5 cut(s) | 699, 1083, 1554, 1842, 2277 |
| BfoI | RGCGCY | 2 cut(s) | 182, 366 |
| BfrI | CTTAAG | 2 cut(s) | 740, 2237 |
| BfuAI | ACCTGC | 1 cut(s) | 1106 |
| BfuI | GTATCC | 1 cut(s) | 1147 |
| BlnI | CCTAGG | 1 cut(s) | 670 |
| BlpI | GCTNAGC | 1 cut(s) | 717 |
| BmcAI | AGTACT | 2 cut(s) | 727, 1147 |
| Bme1390I | CCNGG | 7 cut(s) | 833, 929, 1102, 1662, 2231, 2478, 2517 |
| Bme18I | GGWCC | 3 cut(s) | 1724, 2227, 2442 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2517 |
| BmeT110I | CYCGRG | 1 cut(s) | 2337 |
| BmgBI | CACGTC | 1 cut(s) | 1238 |
| BmgT120I | GGNCC | 4 cut(s) | 619, 1724, 2227, 2442 |
| BmiI | GGNNCC | 6 cut(s) | 180, 621, 933, 2003, 2379, 2694 |
| BmrFI | CCNGG | 7 cut(s) | 833, 929, 1102, 1662, 2231, 2478, 2517 |
| BmrI | ACTGGG | 1 cut(s) | 803 |
| BmsI | GCATC | 1 cut(s) | 2487 |
| BmuI | ACTGGG | 1 cut(s) | 803 |
| BpiI | GAAGAC | 4 cut(s) | 1464, 1811, 2016, 2870 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 145, 177 |
| BpmI | CTGGAG | 1 cut(s) | 1511 |
| Bpu10I | CCTNAGC | 2 cut(s) | 174, 1536 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 717 |
| BpuMI | CCSGG | 2 cut(s) | 833, 2478 |
| Bsa29I | ATCGAT | 1 cut(s) | 2179 |
| BsaAI | YACGTR | 4 cut(s) | 189, 533, 569, 1028 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2169, 2262 |
| BsaHI | GRCGYC | 1 cut(s) | 179 |
| BsaI | GGTCTC | 1 cut(s) | 2848 |
| BsaJI | CCNNGG | 9 cut(s) | 495, 670, 1101, 1272, 1572, 1660, 1819, 2223, 2477 |
| BsaWI | WCCGGW | 2 cut(s) | 439, 1647 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 24, 54, 1737, 1767 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 634, 973, 1249, 1354 |
| Bse118I | RCCGGY | 3 cut(s) | 169, 181, 1647 |
| Bse1I | ACTGG | 8 cut(s) | 613, 798, 1133, 1424, 1494, 2617, 2727, 2860 |
| Bse21I | CCTNAGG | 1 cut(s) | 2418 |
| Bse3DI | GCAATG | 2 cut(s) | 2766, 2778 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2169, 2262 |
| BseBI | CCWGG | 5 cut(s) | 929, 1102, 1662, 2231, 2517 |
| BseCI | ATCGAT | 1 cut(s) | 2179 |
| BseDI | CCNNGG | 9 cut(s) | 495, 670, 1101, 1272, 1572, 1660, 1819, 2223, 2477 |
| BseGI | GGATG | 4 cut(s) | 696, 2502, 2656, 2782 |
| BseJI | GATNNNNATC | 2 cut(s) | 2169, 2262 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 634, 973, 1249, 1354 |
| BseMI | GCAATG | 2 cut(s) | 2766, 2778 |
| BseNI | ACTGG | 8 cut(s) | 613, 798, 1133, 1424, 1494, 2617, 2727, 2860 |
| BseRI | GAGGAG | 3 cut(s) | 107, 166, 1424 |
| BseSI | GKGCMC | 3 cut(s) | 1123, 1171, 1237 |
| BsgI | GTGCAG | 3 cut(s) | 371, 633, 1134 |
| Bsh1285I | CGRYCG | 3 cut(s) | 24, 170, 264 |
| BshFI | GGCC | 9 cut(s) | 63, 185, 259, 275, 620, 1132, 1328, 1953, 2351 |
| BshNI | GGYRCC | 5 cut(s) | 178, 931, 2001, 2377, 2692 |
| BshTI | ACCGGT | 1 cut(s) | 1647 |
| BshVI | ATCGAT | 1 cut(s) | 2179 |
| BsiEI | CGRYCG | 3 cut(s) | 24, 170, 264 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1123, 1171, 1237 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2337 |
| BsiSI | CCGG | 9 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1648, 2477 |
| BslFI | GGGAC | 3 cut(s) | 25, 2067, 2494 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 634, 973, 1249, 1354 |
| BsmAI | GTCTC | 3 cut(s) | 236, 1832, 2848 |
| BsmFI | GGGAC | 3 cut(s) | 25, 2067, 2494 |
| BsmI | GAATGC | 1 cut(s) | 2242 |
| BsnI | GGCC | 9 cut(s) | 63, 185, 259, 275, 620, 1132, 1328, 1953, 2351 |
| Bso31I | GGTCTC | 1 cut(s) | 2848 |
| BsoBI | CYCGRG | 1 cut(s) | 2337 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1123, 1171, 1237 |
| Bsp143I | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 717 |
| Bsp19I | CCATGG | 3 cut(s) | 1272, 1819, 2223 |
| BspANI | GGCC | 9 cut(s) | 63, 185, 259, 275, 620, 1132, 1328, 1953, 2351 |
| BspDI | ATCGAT | 1 cut(s) | 2179 |
| BspHI | TCATGA | 1 cut(s) | 1390 |
| BspLI | GGNNCC | 6 cut(s) | 180, 621, 933, 2003, 2379, 2694 |
| BspMAI | CTGCAG | 3 cut(s) | 703, 1558, 1846 |
| BspMI | ACCTGC | 1 cut(s) | 1106 |
| BspPI | GGATC | 5 cut(s) | 200, 1017, 1390, 1430, 2172 |
| BspQI | GCTCTTC | 1 cut(s) | 1728 |
| BspT107I | GGYRCC | 5 cut(s) | 178, 931, 2001, 2377, 2692 |
| BspTI | CTTAAG | 2 cut(s) | 740, 2237 |
| BspTNI | GGTCTC | 1 cut(s) | 2848 |
| BsrBI | CCGCTC | 2 cut(s) | 120, 969 |
| BsrDI | GCAATG | 2 cut(s) | 2766, 2778 |
| BsrFI | RCCGGY | 3 cut(s) | 169, 181, 1647 |
| BsrI | ACTGG | 8 cut(s) | 613, 798, 1133, 1424, 1494, 2617, 2727, 2860 |
| BssAI | RCCGGY | 3 cut(s) | 169, 181, 1647 |
| BssECI | CCNNGG | 9 cut(s) | 495, 670, 1101, 1272, 1572, 1660, 1819, 2223, 2477 |
| BssMI | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| BssNAI | GTATAC | 1 cut(s) | 768 |
| BssNI | GRCGYC | 1 cut(s) | 179 |
| BssT1I | CCWWGG | 5 cut(s) | 495, 670, 1272, 1819, 2223 |
| Bst1107I | GTATAC | 1 cut(s) | 768 |
| Bst2UI | CCWGG | 5 cut(s) | 929, 1102, 1662, 2231, 2517 |
| Bst4CI | ACNGT | 4 cut(s) | 686, 772, 2049, 2395 |
| Bst6I | CTCTTC | 4 cut(s) | 1401, 1627, 1728, 2755 |
| BstACI | GRCGYC | 1 cut(s) | 179 |
| BstAFI | CTTAAG | 2 cut(s) | 740, 2237 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 611, 1420, 2086, 2735 |
| BstBAI | YACGTR | 4 cut(s) | 189, 533, 569, 1028 |
| BstC8I | GCNNGC | 5 cut(s) | 183, 516, 737, 893, 1167 |
| BstDSI | CCRYGG | 3 cut(s) | 1272, 1819, 2223 |
| BstF5I | GGATG | 4 cut(s) | 696, 2502, 2656, 2782 |
| BstH2I | RGCGCY | 2 cut(s) | 182, 366 |
| BstHHI | GCGC | 3 cut(s) | 181, 365, 505 |
| BstKTI | GATC | 9 cut(s) | 143, 195, 264, 1012, 1385, 1438, 1800, 2019, 2167 |
| BstMAI | GTCTC | 3 cut(s) | 236, 1832, 2848 |
| BstMBI | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| BstMCI | CGRYCG | 3 cut(s) | 24, 170, 264 |
| BstNI | CCWGG | 5 cut(s) | 929, 1102, 1662, 2231, 2517 |
| BstNSI | RCATGY | 4 cut(s) | 58, 1742, 2327, 2733 |
| BstSCI | CCNGG | 7 cut(s) | 831, 927, 1100, 1660, 2229, 2476, 2515 |
| BstSFI | CTRYAG | 5 cut(s) | 699, 1083, 1554, 1842, 2277 |
| BstSLI | GKGCMC | 3 cut(s) | 1123, 1171, 1237 |
| BstV2I | GAAGAC | 4 cut(s) | 1464, 1811, 2016, 2870 |
| BstX2I | RGATCY | 1 cut(s) | 1435 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2839 |
| BstYI | RGATCY | 1 cut(s) | 1435 |
| BstZ17I | GTATAC | 1 cut(s) | 768 |
| Bsu15I | ATCGAT | 1 cut(s) | 2179 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2418 |
| BsuI | GTATCC | 1 cut(s) | 1147 |
| BsuRI | GGCC | 9 cut(s) | 63, 185, 259, 275, 620, 1132, 1328, 1953, 2351 |
| BsuTUI | ATCGAT | 1 cut(s) | 2179 |
| BtgI | CCRYGG | 3 cut(s) | 1272, 1819, 2223 |
| BtgZI | GCGATG | 1 cut(s) | 2805 |
| BtrI | CACGTC | 1 cut(s) | 1238 |
| BtsCI | GGATG | 4 cut(s) | 696, 2502, 2656, 2782 |
| BtsI | GCAGTG | 1 cut(s) | 621 |
| BtsIMutI | CAGTG | 5 cut(s) | 621, 682, 791, 1431, 2054 |
| BveI | ACCTGC | 1 cut(s) | 1106 |
| Cac8I | GCNNGC | 5 cut(s) | 183, 516, 737, 893, 1167 |
| CaiI | CAGNNNCTG | 3 cut(s) | 887, 953, 1058 |
| CciI | TCATGA | 1 cut(s) | 1390 |
| CfoI | GCGC | 3 cut(s) | 181, 365, 505 |
| Cfr10I | RCCGGY | 3 cut(s) | 169, 181, 1647 |
| Cfr13I | GGNCC | 4 cut(s) | 619, 1724, 2227, 2442 |
| ClaI | ATCGAT | 1 cut(s) | 2179 |
| CseI | GACGC | 2 cut(s) | 60, 415 |
| CsiI | ACCWGGT | 2 cut(s) | 927, 2515 |
| Csp6I | GTAC | 7 cut(s) | 49, 573, 726, 804, 1146, 2274, 2378 |
| CspAI | ACCGGT | 1 cut(s) | 1647 |
| CviQI | GTAC | 7 cut(s) | 49, 573, 726, 804, 1146, 2274, 2378 |
| DinI | GGCGCC | 1 cut(s) | 180 |
| DpnI | GATC | 9 cut(s) | 142, 194, 263, 1011, 1384, 1437, 1799, 2018, 2166 |
| DpnII | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| DraI | TTTAAA | 1 cut(s) | 2152 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2621 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2517 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2621 |
| EaeI | YGGCCR | 4 cut(s) | 61, 183, 257, 1326 |
| Eam1104I | CTCTTC | 4 cut(s) | 1401, 1627, 1728, 2755 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2517 |
| EarI | CTCTTC | 4 cut(s) | 1401, 1627, 1728, 2755 |
| Eco130I | CCWWGG | 5 cut(s) | 495, 670, 1272, 1819, 2223 |
| Eco31I | GGTCTC | 1 cut(s) | 2848 |
| Eco47I | GGWCC | 3 cut(s) | 1724, 2227, 2442 |
| Eco57I | CTGAAG | 4 cut(s) | 470, 530, 1488, 2343 |
| Eco72I | CACGTG | 2 cut(s) | 189, 1028 |
| Eco81I | CCTNAGG | 1 cut(s) | 2418 |
| Eco88I | CYCGRG | 1 cut(s) | 2337 |
| EcoRII | CCWGG | 5 cut(s) | 927, 1100, 1660, 2229, 2515 |
| EcoT14I | CCWWGG | 5 cut(s) | 495, 670, 1272, 1819, 2223 |
| EgeI | GGCGCC | 1 cut(s) | 180 |
| EheI | GGCGCC | 1 cut(s) | 180 |
| ErhI | CCWWGG | 5 cut(s) | 495, 670, 1272, 1819, 2223 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 465, 497, 2186, 2218, 2396, 2428 |
| FaqI | GGGAC | 3 cut(s) | 25, 2067, 2494 |
| FauI | CCCGC | 1 cut(s) | 999 |
| FblI | GTMKAC | 4 cut(s) | 767, 1075, 2397, 2464 |
| FokI | GGATG | 4 cut(s) | 703, 2509, 2663, 2789 |
| FspBI | CTAG | 6 cut(s) | 147, 248, 671, 1089, 1184, 2630 |
| GlaI | GCGC | 3 cut(s) | 180, 364, 504 |
| GsuI | CTGGAG | 1 cut(s) | 1511 |
| HaeII | RGCGCY | 2 cut(s) | 182, 366 |
| HaeIII | GGCC | 9 cut(s) | 63, 185, 259, 275, 620, 1132, 1328, 1953, 2351 |
| HapII | CCGG | 9 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1648, 2477 |
| HgaI | GACGC | 2 cut(s) | 60, 415 |
| HhaI | GCGC | 3 cut(s) | 181, 365, 505 |
| Hin1I | GRCGYC | 1 cut(s) | 179 |
| Hin6I | GCGC | 3 cut(s) | 179, 363, 503 |
| HinP1I | GCGC | 3 cut(s) | 179, 363, 503 |
| HincII | GTYRAC | 5 cut(s) | 405, 819, 1906, 2286, 2398 |
| HindII | GTYRAC | 5 cut(s) | 405, 819, 1906, 2286, 2398 |
| HindIII | AAGCTT | 1 cut(s) | 1290 |
| HpaI | GTTAAC | 1 cut(s) | 819 |
| HpaII | CCGG | 9 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1648, 2477 |
| HphI | GGTGA | 9 cut(s) | 514, 764, 1289, 1484, 1835, 2315, 2342, 2555, 2798 |
| Hpy188III | TCNNGA | 8 cut(s) | 138, 761, 1391, 1795, 1801, 1970, 2420, 2532 |
| Hpy99I | CGWCG | 6 cut(s) | 76, 79, 104, 107, 110, 1242 |
| HpyAV | CCTTC | 9 cut(s) | 73, 286, 491, 1325, 1463, 1489, 1852, 2100, 2433 |
| HpyCH4III | ACNGT | 4 cut(s) | 686, 772, 2049, 2395 |
| HpyCH4IV | ACGT | 5 cut(s) | 188, 532, 568, 1027, 1237 |
| HpySE526I | ACGT | 5 cut(s) | 188, 532, 568, 1027, 1237 |
| Hsp92I | GRCGYC | 1 cut(s) | 179 |
| HspAI | GCGC | 3 cut(s) | 179, 363, 503 |
| KasI | GGCGCC | 1 cut(s) | 178 |
| KpnI | GGTACC | 1 cut(s) | 2381 |
| KroI | GCCGGC | 1 cut(s) | 181 |
| KroNI | GCCGGC | 1 cut(s) | 183 |
| KspAI | GTTAAC | 1 cut(s) | 819 |
| Kzo9I | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| LguI | GCTCTTC | 1 cut(s) | 1728 |
| LmnI | GCTCC | 7 cut(s) | 32, 117, 1411, 1498, 1946, 2233, 2370 |
| LweI | GCATC | 1 cut(s) | 2487 |
| MabI | ACCWGGT | 2 cut(s) | 927, 2515 |
| MaeI | CTAG | 6 cut(s) | 147, 248, 671, 1089, 1184, 2630 |
| MaeII | ACGT | 5 cut(s) | 188, 532, 568, 1027, 1237 |
| MaeIII | GTNAC | 3 cut(s) | 976, 1687, 2543 |
| MalI | GATC | 9 cut(s) | 142, 194, 263, 1011, 1384, 1437, 1799, 2018, 2166 |
| MbiI | CCGCTC | 2 cut(s) | 120, 969 |
| MboI | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| MfeI | CAATTG | 1 cut(s) | 2107 |
| MflI | RGATCY | 1 cut(s) | 1435 |
| MhlI | GDGCHC | 3 cut(s) | 1123, 1171, 1237 |
| Mly113I | GGCGCC | 1 cut(s) | 179 |
| MlyI | GAGTC | 4 cut(s) | 206, 238, 461, 1901 |
| MmeI | TCCRAC | 5 cut(s) | 689, 763, 1463, 1723, 2685 |
| MroNI | GCCGGC | 1 cut(s) | 181 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1201 |
| MslI | CAYNNNNRTG | 6 cut(s) | 648, 1026, 1824, 2328, 2771, 2872 |
| MspA1I | CMGCKG | 2 cut(s) | 809, 1343 |
| MspCI | CTTAAG | 2 cut(s) | 740, 2237 |
| MspI | CCGG | 9 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1648, 2477 |
| MspR9I | CCNGG | 7 cut(s) | 833, 929, 1102, 1662, 2231, 2478, 2517 |
| MunI | CAATTG | 1 cut(s) | 2107 |
| Mva1269I | GAATGC | 1 cut(s) | 2242 |
| MvaI | CCWGG | 5 cut(s) | 929, 1102, 1662, 2231, 2517 |
| NaeI | GCCGGC | 1 cut(s) | 183 |
| NarI | GGCGCC | 1 cut(s) | 179 |
| NciI | CCSGG | 2 cut(s) | 833, 2478 |
| NcoI | CCATGG | 3 cut(s) | 1272, 1819, 2223 |
| NdeII | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| NgoMIV | GCCGGC | 1 cut(s) | 181 |
| NlaIV | GGNNCC | 6 cut(s) | 180, 621, 933, 2003, 2379, 2694 |
| NmeAIII | GCCGAG | 1 cut(s) | 142 |
| NmuCI | GTSAC | 2 cut(s) | 976, 2543 |
| NspI | RCATGY | 4 cut(s) | 58, 1742, 2327, 2733 |
| PagI | TCATGA | 1 cut(s) | 1390 |
| PaqCI | CACCTGC | 1 cut(s) | 1106 |
| PciI | ACATGT | 2 cut(s) | 54, 2323 |
| PciSI | GCTCTTC | 1 cut(s) | 1728 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 105 |
| PctI | GAATGC | 1 cut(s) | 2242 |
| PdiI | GCCGGC | 1 cut(s) | 183 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1201 |
| PfoI | TCCNGGA | 1 cut(s) | 831 |
| PinAI | ACCGGT | 1 cut(s) | 1647 |
| Ple19I | CGATCG | 1 cut(s) | 264 |
| PleI | GAGTC | 4 cut(s) | 205, 238, 460, 1901 |
| PluTI | GGCGCC | 1 cut(s) | 182 |
| PmaCI | CACGTG | 2 cut(s) | 189, 1028 |
| PmlI | CACGTG | 2 cut(s) | 189, 1028 |
| PpsI | GAGTC | 4 cut(s) | 205, 238, 460, 1901 |
| Ppu21I | YACGTR | 4 cut(s) | 189, 533, 569, 1028 |
| PscI | ACATGT | 2 cut(s) | 54, 2323 |
| Psp6I | CCWGG | 5 cut(s) | 927, 1100, 1660, 2229, 2515 |
| PspCI | CACGTG | 2 cut(s) | 189, 1028 |
| PspGI | CCWGG | 5 cut(s) | 927, 1100, 1660, 2229, 2515 |
| PspN4I | GGNNCC | 6 cut(s) | 180, 621, 933, 2003, 2379, 2694 |
| PspPI | GGNCC | 4 cut(s) | 619, 1724, 2227, 2442 |
| PstI | CTGCAG | 3 cut(s) | 703, 1558, 1846 |
| PstNI | CAGNNNCTG | 3 cut(s) | 887, 953, 1058 |
| PsuI | RGATCY | 1 cut(s) | 1435 |
| PvuI | CGATCG | 1 cut(s) | 264 |
| PvuII | CAGCTG | 1 cut(s) | 809 |
| RsaI | GTAC | 7 cut(s) | 50, 574, 727, 805, 1147, 2275, 2379 |
| RsaNI | GTAC | 7 cut(s) | 49, 573, 726, 804, 1146, 2274, 2378 |
| RseI | CAYNNNNRTG | 6 cut(s) | 648, 1026, 1824, 2328, 2771, 2872 |
| SalI | GTCGAC | 1 cut(s) | 2396 |
| SapI | GCTCTTC | 1 cut(s) | 1728 |
| Sau3AI | GATC | 9 cut(s) | 140, 192, 261, 1009, 1382, 1435, 1797, 2016, 2164 |
| Sau96I | GGNCC | 4 cut(s) | 619, 1724, 2227, 2442 |
| ScaI | AGTACT | 2 cut(s) | 727, 1147 |
| SchI | GAGTC | 4 cut(s) | 206, 238, 461, 1901 |
| ScrFI | CCNGG | 7 cut(s) | 833, 929, 1102, 1662, 2231, 2478, 2517 |
| SduI | GDGCHC | 3 cut(s) | 1123, 1171, 1237 |
| SexAI | ACCWGGT | 2 cut(s) | 927, 2515 |
| SfaNI | GCATC | 1 cut(s) | 2487 |
| SfcI | CTRYAG | 5 cut(s) | 699, 1083, 1554, 1842, 2277 |
| SfoI | GGCGCC | 1 cut(s) | 180 |
| SinI | GGWCC | 3 cut(s) | 1724, 2227, 2442 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 648, 1026, 1824, 2328, 2771, 2872 |
| SmlI | CTYRAG | 2 cut(s) | 740, 2237 |
| SmoI | CTYRAG | 2 cut(s) | 740, 2237 |
| SspDI | GGCGCC | 1 cut(s) | 178 |
| SspMI | CTAG | 6 cut(s) | 147, 248, 671, 1089, 1184, 2630 |
| StyD4I | CCNGG | 7 cut(s) | 831, 927, 1100, 1660, 2229, 2476, 2515 |
| StyI | CCWWGG | 5 cut(s) | 495, 670, 1272, 1819, 2223 |
| TaaI | ACNGT | 4 cut(s) | 686, 772, 2049, 2395 |
| TaiI | ACGT | 5 cut(s) | 191, 535, 571, 1030, 1240 |
| TaqII | GACCGA | 1 cut(s) | 1741 |
| TatI | WGTACW | 3 cut(s) | 48, 725, 1145 |
| TauI | GCSGC | 2 cut(s) | 8, 29 |
| TscAI | CASTG | 5 cut(s) | 621, 689, 798, 1431, 2054 |
| TseFI | GTSAC | 2 cut(s) | 976, 2543 |
| Tsp45I | GTSAC | 2 cut(s) | 976, 2543 |
| TspRI | CASTG | 5 cut(s) | 621, 689, 798, 1431, 2054 |
| Vha464I | CTTAAG | 2 cut(s) | 740, 2237 |
| VneI | GTGCAC | 3 cut(s) | 1119, 1167, 1233 |
| VpaK11BI | GGWCC | 3 cut(s) | 1724, 2227, 2442 |
| XapI | RAATTY | 3 cut(s) | 310, 1994, 2146 |
| XceI | RCATGY | 4 cut(s) | 58, 1742, 2327, 2733 |
| XmaJI | CCTAGG | 1 cut(s) | 670 |
| XmiI | GTMKAC | 4 cut(s) | 767, 1075, 2397, 2464 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1201 |
| XspI | CTAG | 6 cut(s) | 147, 248, 671, 1089, 1184, 2630 |
| ZrmI | AGTACT | 2 cut(s) | 727, 1147 |