Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1130 |
| Acc16I | TGCGCA | 1 cut(s) | 1194 |
| Acc36I | ACCTGC | 1 cut(s) | 1130 |
| AccB1I | GGYRCC | 2 cut(s) | 178, 955 |
| AccBSI | CCGCTC | 2 cut(s) | 120, 993 |
| AccI | GTMKAC | 2 cut(s) | 767, 1099 |
| AclWI | GGATC | 4 cut(s) | 200, 1041, 1414, 1454 |
| AcoI | YGGCCR | 4 cut(s) | 61, 183, 257, 1350 |
| AcsI | RAATTY | 1 cut(s) | 310 |
| AcuI | CTGAAG | 3 cut(s) | 470, 530, 1512 |
| AcvI | CACGTG | 2 cut(s) | 189, 1052 |
| AcyI | GRCGYC | 1 cut(s) | 179 |
| AfaI | GTAC | 7 cut(s) | 50, 574, 727, 805, 859, 1171, 1796 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 634, 997, 1273, 1378 |
| AflII | CTTAAG | 1 cut(s) | 740 |
| AflIII | ACRYGT | 4 cut(s) | 54, 1049, 1051, 1781 |
| AgeI | ACCGGT | 1 cut(s) | 1671 |
| AgsI | TTSAA | 4 cut(s) | 546, 855, 1060, 1288 |
| AjiI | CACGTC | 1 cut(s) | 1262 |
| AjnI | CCWGG | 3 cut(s) | 951, 1124, 1684 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1749, 1781 |
| Alw21I | GWGCWC | 2 cut(s) | 1147, 1261 |
| Alw26I | GTCTC | 2 cut(s) | 236, 1772 |
| Alw44I | GTGCAC | 2 cut(s) | 1143, 1257 |
| AlwI | GGATC | 4 cut(s) | 200, 1041, 1414, 1454 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 911, 977, 1082 |
| AoxI | GGCC | 7 cut(s) | 61, 183, 257, 273, 618, 1154, 1350 |
| ApaLI | GTGCAC | 2 cut(s) | 1143, 1257 |
| ApoI | RAATTY | 1 cut(s) | 310 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 260, 292 |
| AsiGI | ACCGGT | 1 cut(s) | 1671 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1225 |
| AspA2I | CCTAGG | 1 cut(s) | 670 |
| AspLEI | GCGC | 4 cut(s) | 181, 365, 505, 1195 |
| AspS9I | GGNCC | 2 cut(s) | 619, 1748 |
| AsuC2I | CCSGG | 1 cut(s) | 833 |
| AsuHPI | GGTGA | 2 cut(s) | 1313, 1508 |
| AvaII | GGWCC | 1 cut(s) | 1748 |
| AvrII | CCTAGG | 1 cut(s) | 670 |
| BaeGI | GKGCMC | 2 cut(s) | 1147, 1261 |
| BanI | GGYRCC | 2 cut(s) | 178, 955 |
| BbrPI | CACGTG | 2 cut(s) | 189, 1052 |
| BbsI | GAAGAC | 1 cut(s) | 1488 |
| Bbv12I | GWGCWC | 2 cut(s) | 1147, 1261 |
| BccI | CCATC | 5 cut(s) | 641, 685, 1111, 1739, 1855 |
| BceAI | ACGGC | 2 cut(s) | 100, 1251 |
| BciT130I | CCWGG | 3 cut(s) | 953, 1126, 1686 |
| BciVI | GTATCC | 1 cut(s) | 1171 |
| BclI | TGATCA | 1 cut(s) | 1824 |
| BcnI | CCSGG | 1 cut(s) | 833 |
| BcoDI | GTCTC | 2 cut(s) | 236, 1772 |
| BfaI | CTAG | 6 cut(s) | 147, 248, 671, 1113, 1208, 1815 |
| BfmI | CTRYAG | 3 cut(s) | 699, 1107, 1578 |
| BfoI | RGCGCY | 2 cut(s) | 182, 366 |
| BfrI | CTTAAG | 1 cut(s) | 740 |
| BfuAI | ACCTGC | 1 cut(s) | 1130 |
| BfuI | GTATCC | 1 cut(s) | 1171 |
| BlnI | CCTAGG | 1 cut(s) | 670 |
| BlpI | GCTNAGC | 1 cut(s) | 717 |
| BmcAI | AGTACT | 3 cut(s) | 727, 859, 1171 |
| Bme1390I | CCNGG | 4 cut(s) | 833, 953, 1126, 1686 |
| Bme18I | GGWCC | 1 cut(s) | 1748 |
| BmgBI | CACGTC | 1 cut(s) | 1262 |
| BmgT120I | GGNCC | 2 cut(s) | 619, 1748 |
| BmiI | GGNNCC | 3 cut(s) | 180, 621, 957 |
| BmrFI | CCNGG | 4 cut(s) | 833, 953, 1126, 1686 |
| BmrI | ACTGGG | 1 cut(s) | 803 |
| BmuI | ACTGGG | 1 cut(s) | 803 |
| BpiI | GAAGAC | 1 cut(s) | 1488 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 145, 177 |
| BpmI | CTGGAG | 1 cut(s) | 1535 |
| Bpu10I | CCTNAGC | 2 cut(s) | 174, 1560 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 717 |
| BpuMI | CCSGG | 1 cut(s) | 833 |
| BsaAI | YACGTR | 5 cut(s) | 189, 533, 569, 1052, 1784 |
| BsaHI | GRCGYC | 1 cut(s) | 179 |
| BsaJI | CCNNGG | 6 cut(s) | 495, 670, 1125, 1296, 1596, 1684 |
| BsaWI | WCCGGW | 2 cut(s) | 439, 1671 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 24, 54 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 634, 997, 1273, 1378 |
| Bse118I | RCCGGY | 3 cut(s) | 169, 181, 1671 |
| Bse1I | ACTGG | 6 cut(s) | 613, 798, 1157, 1448, 1518, 1887 |
| BseBI | CCWGG | 3 cut(s) | 953, 1126, 1686 |
| BseDI | CCNNGG | 6 cut(s) | 495, 670, 1125, 1296, 1596, 1684 |
| BseGI | GGATG | 1 cut(s) | 696 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 634, 997, 1273, 1378 |
| BseMII | CTCAG | 8 cut(s) | 188, 231, 736, 753, 981, 987, 1187, 1653 |
| BseNI | ACTGG | 6 cut(s) | 613, 798, 1157, 1448, 1518, 1887 |
| BseRI | GAGGAG | 2 cut(s) | 166, 1448 |
| BseSI | GKGCMC | 2 cut(s) | 1147, 1261 |
| BseYI | CCCAGC | 1 cut(s) | 877 |
| BsgI | GTGCAG | 3 cut(s) | 371, 633, 1158 |
| Bsh1285I | CGRYCG | 3 cut(s) | 24, 170, 264 |
| BshFI | GGCC | 7 cut(s) | 63, 185, 259, 275, 620, 1156, 1352 |
| BshNI | GGYRCC | 2 cut(s) | 178, 955 |
| BshTI | ACCGGT | 1 cut(s) | 1671 |
| BsiEI | CGRYCG | 3 cut(s) | 24, 170, 264 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1147, 1261 |
| BsiSI | CCGG | 8 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1672 |
| BslFI | GGGAC | 1 cut(s) | 25 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 634, 997, 1273, 1378 |
| BsmAI | GTCTC | 2 cut(s) | 236, 1772 |
| BsmFI | GGGAC | 1 cut(s) | 25 |
| BsnI | GGCC | 7 cut(s) | 63, 185, 259, 275, 620, 1156, 1352 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1147, 1261 |
| Bsp1407I | TGTACA | 1 cut(s) | 1794 |
| Bsp143I | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 717 |
| Bsp19I | CCATGG | 1 cut(s) | 1296 |
| BspANI | GGCC | 7 cut(s) | 63, 185, 259, 275, 620, 1156, 1352 |
| BspCNI | CTCAG | 8 cut(s) | 187, 232, 735, 754, 980, 988, 1186, 1654 |
| BspHI | TCATGA | 1 cut(s) | 1414 |
| BspLI | GGNNCC | 3 cut(s) | 180, 621, 957 |
| BspMAI | CTGCAG | 2 cut(s) | 703, 1582 |
| BspMI | ACCTGC | 1 cut(s) | 1130 |
| BspPI | GGATC | 4 cut(s) | 200, 1041, 1414, 1454 |
| BspQI | GCTCTTC | 1 cut(s) | 1752 |
| BspT107I | GGYRCC | 2 cut(s) | 178, 955 |
| BspTI | CTTAAG | 1 cut(s) | 740 |
| BsrBI | CCGCTC | 2 cut(s) | 120, 993 |
| BsrFI | RCCGGY | 3 cut(s) | 169, 181, 1671 |
| BsrGI | TGTACA | 1 cut(s) | 1794 |
| BsrI | ACTGG | 6 cut(s) | 613, 798, 1157, 1448, 1518, 1887 |
| BssAI | RCCGGY | 3 cut(s) | 169, 181, 1671 |
| BssECI | CCNNGG | 6 cut(s) | 495, 670, 1125, 1296, 1596, 1684 |
| BssMI | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| BssNAI | GTATAC | 1 cut(s) | 768 |
| BssNI | GRCGYC | 1 cut(s) | 179 |
| BssT1I | CCWWGG | 3 cut(s) | 495, 670, 1296 |
| Bst1107I | GTATAC | 1 cut(s) | 768 |
| Bst2UI | CCWGG | 3 cut(s) | 953, 1126, 1686 |
| Bst4CI | ACNGT | 5 cut(s) | 435, 686, 772, 1794, 1799 |
| Bst6I | CTCTTC | 3 cut(s) | 1425, 1651, 1752 |
| BstACI | GRCGYC | 1 cut(s) | 179 |
| BstAFI | CTTAAG | 1 cut(s) | 740 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 611, 1444 |
| BstAUI | TGTACA | 1 cut(s) | 1794 |
| BstBAI | YACGTR | 5 cut(s) | 189, 533, 569, 1052, 1784 |
| BstC8I | GCNNGC | 5 cut(s) | 183, 516, 737, 917, 1191 |
| BstDSI | CCRYGG | 1 cut(s) | 1296 |
| BstF5I | GGATG | 1 cut(s) | 696 |
| BstH2I | RGCGCY | 2 cut(s) | 182, 366 |
| BstHHI | GCGC | 4 cut(s) | 181, 365, 505, 1195 |
| BstKTI | GATC | 8 cut(s) | 143, 195, 264, 1036, 1409, 1462, 1827, 1834 |
| BstMAI | GTCTC | 2 cut(s) | 236, 1772 |
| BstMBI | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| BstMCI | CGRYCG | 3 cut(s) | 24, 170, 264 |
| BstNI | CCWGG | 3 cut(s) | 953, 1126, 1686 |
| BstNSI | RCATGY | 2 cut(s) | 58, 1766 |
| BstSCI | CCNGG | 4 cut(s) | 831, 951, 1124, 1684 |
| BstSFI | CTRYAG | 3 cut(s) | 699, 1107, 1578 |
| BstSLI | GKGCMC | 2 cut(s) | 1147, 1261 |
| BstV2I | GAAGAC | 1 cut(s) | 1488 |
| BstX2I | RGATCY | 1 cut(s) | 1459 |
| BstYI | RGATCY | 1 cut(s) | 1459 |
| BstZ17I | GTATAC | 1 cut(s) | 768 |
| BsuI | GTATCC | 1 cut(s) | 1171 |
| BsuRI | GGCC | 7 cut(s) | 63, 185, 259, 275, 620, 1156, 1352 |
| BtgI | CCRYGG | 1 cut(s) | 1296 |
| BtrI | CACGTC | 1 cut(s) | 1262 |
| BtsCI | GGATG | 1 cut(s) | 696 |
| BtsI | GCAGTG | 1 cut(s) | 621 |
| BtsIMutI | CAGTG | 4 cut(s) | 621, 682, 791, 1455 |
| BveI | ACCTGC | 1 cut(s) | 1130 |
| Cac8I | GCNNGC | 5 cut(s) | 183, 516, 737, 917, 1191 |
| CaiI | CAGNNNCTG | 3 cut(s) | 911, 977, 1082 |
| CciI | TCATGA | 1 cut(s) | 1414 |
| CfoI | GCGC | 4 cut(s) | 181, 365, 505, 1195 |
| Cfr10I | RCCGGY | 3 cut(s) | 169, 181, 1671 |
| Cfr13I | GGNCC | 2 cut(s) | 619, 1748 |
| CseI | GACGC | 2 cut(s) | 60, 415 |
| CsiI | ACCWGGT | 1 cut(s) | 951 |
| Csp6I | GTAC | 7 cut(s) | 49, 573, 726, 804, 858, 1170, 1795 |
| CspAI | ACCGGT | 1 cut(s) | 1671 |
| CviQI | GTAC | 7 cut(s) | 49, 573, 726, 804, 858, 1170, 1795 |
| DinI | GGCGCC | 1 cut(s) | 180 |
| DpnI | GATC | 8 cut(s) | 142, 194, 263, 1035, 1408, 1461, 1826, 1833 |
| DpnII | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| EaeI | YGGCCR | 4 cut(s) | 61, 183, 257, 1350 |
| Eam1104I | CTCTTC | 3 cut(s) | 1425, 1651, 1752 |
| EarI | CTCTTC | 3 cut(s) | 1425, 1651, 1752 |
| Eco130I | CCWWGG | 3 cut(s) | 495, 670, 1296 |
| Eco47I | GGWCC | 1 cut(s) | 1748 |
| Eco57I | CTGAAG | 3 cut(s) | 470, 530, 1512 |
| Eco72I | CACGTG | 2 cut(s) | 189, 1052 |
| EcoRII | CCWGG | 3 cut(s) | 951, 1124, 1684 |
| EcoT14I | CCWWGG | 3 cut(s) | 495, 670, 1296 |
| EgeI | GGCGCC | 1 cut(s) | 180 |
| EheI | GGCGCC | 1 cut(s) | 180 |
| ErhI | CCWWGG | 3 cut(s) | 495, 670, 1296 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 465, 497 |
| FaqI | GGGAC | 1 cut(s) | 25 |
| FauI | CCCGC | 1 cut(s) | 1023 |
| FbaI | TGATCA | 1 cut(s) | 1824 |
| FblI | GTMKAC | 2 cut(s) | 767, 1099 |
| FokI | GGATG | 1 cut(s) | 703 |
| FspBI | CTAG | 6 cut(s) | 147, 248, 671, 1113, 1208, 1815 |
| FspI | TGCGCA | 1 cut(s) | 1194 |
| GlaI | GCGC | 4 cut(s) | 180, 364, 504, 1194 |
| GsaI | CCCAGC | 1 cut(s) | 881 |
| GsuI | CTGGAG | 1 cut(s) | 1535 |
| HaeII | RGCGCY | 2 cut(s) | 182, 366 |
| HaeIII | GGCC | 7 cut(s) | 63, 185, 259, 275, 620, 1156, 1352 |
| HapII | CCGG | 8 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1672 |
| HgaI | GACGC | 2 cut(s) | 60, 415 |
| HhaI | GCGC | 4 cut(s) | 181, 365, 505, 1195 |
| Hin1I | GRCGYC | 1 cut(s) | 179 |
| Hin6I | GCGC | 4 cut(s) | 179, 363, 503, 1193 |
| HinP1I | GCGC | 4 cut(s) | 179, 363, 503, 1193 |
| HincII | GTYRAC | 1 cut(s) | 405 |
| HindII | GTYRAC | 1 cut(s) | 405 |
| HpaII | CCGG | 8 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1672 |
| HphI | GGTGA | 2 cut(s) | 1313, 1508 |
| Hpy166II | GTNNAC | 8 cut(s) | 207, 405, 522, 575, 768, 1100, 1145, 1259 |
| Hpy188III | TCNNGA | 4 cut(s) | 138, 761, 1415, 1815 |
| Hpy8I | GTNNAC | 8 cut(s) | 207, 405, 522, 575, 768, 1100, 1145, 1259 |
| Hpy99I | CGWCG | 7 cut(s) | 76, 79, 101, 104, 107, 110, 1266 |
| HpyAV | CCTTC | 7 cut(s) | 73, 286, 491, 566, 1349, 1487, 1513 |
| HpyCH4III | ACNGT | 5 cut(s) | 435, 686, 772, 1794, 1799 |
| HpyCH4IV | ACGT | 6 cut(s) | 188, 532, 568, 1051, 1261, 1783 |
| HpySE526I | ACGT | 6 cut(s) | 188, 532, 568, 1051, 1261, 1783 |
| Hsp92I | GRCGYC | 1 cut(s) | 179 |
| HspAI | GCGC | 4 cut(s) | 179, 363, 503, 1193 |
| KasI | GGCGCC | 1 cut(s) | 178 |
| KroI | GCCGGC | 1 cut(s) | 181 |
| KroNI | GCCGGC | 1 cut(s) | 183 |
| Ksp22I | TGATCA | 1 cut(s) | 1824 |
| Kzo9I | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| LguI | GCTCTTC | 1 cut(s) | 1752 |
| LmnI | GCTCC | 5 cut(s) | 32, 117, 1435, 1522, 1809 |
| MabI | ACCWGGT | 1 cut(s) | 951 |
| MaeI | CTAG | 6 cut(s) | 147, 248, 671, 1113, 1208, 1815 |
| MaeII | ACGT | 6 cut(s) | 188, 532, 568, 1051, 1261, 1783 |
| MaeIII | GTNAC | 2 cut(s) | 1000, 1711 |
| MalI | GATC | 8 cut(s) | 142, 194, 263, 1035, 1408, 1461, 1826, 1833 |
| MbiI | CCGCTC | 2 cut(s) | 120, 993 |
| MboI | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| MflI | RGATCY | 1 cut(s) | 1459 |
| MhlI | GDGCHC | 2 cut(s) | 1147, 1261 |
| MluCI | AATT | 7 cut(s) | 310, 385, 525, 845, 1384, 1499, 1865 |
| Mly113I | GGCGCC | 1 cut(s) | 179 |
| MlyI | GAGTC | 3 cut(s) | 206, 238, 461 |
| MmeI | TCCRAC | 4 cut(s) | 689, 763, 1487, 1831 |
| MroNI | GCCGGC | 1 cut(s) | 181 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1225 |
| MseI | TTAA | 6 cut(s) | 285, 395, 741, 818, 1019, 1584 |
| MslI | CAYNNNNRTG | 2 cut(s) | 648, 1050 |
| MspA1I | CMGCKG | 3 cut(s) | 809, 881, 1367 |
| MspCI | CTTAAG | 1 cut(s) | 740 |
| MspI | CCGG | 8 cut(s) | 30, 64, 170, 182, 440, 456, 832, 1672 |
| MspR9I | CCNGG | 4 cut(s) | 833, 953, 1126, 1686 |
| MvaI | CCWGG | 3 cut(s) | 953, 1126, 1686 |
| NaeI | GCCGGC | 1 cut(s) | 183 |
| NarI | GGCGCC | 1 cut(s) | 179 |
| NciI | CCSGG | 1 cut(s) | 833 |
| NcoI | CCATGG | 1 cut(s) | 1296 |
| NdeII | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| NgoMIV | GCCGGC | 1 cut(s) | 181 |
| NlaIV | GGNNCC | 3 cut(s) | 180, 621, 957 |
| NmeAIII | GCCGAG | 1 cut(s) | 142 |
| NmuCI | GTSAC | 1 cut(s) | 1000 |
| NsbI | TGCGCA | 1 cut(s) | 1194 |
| NspI | RCATGY | 2 cut(s) | 58, 1766 |
| PagI | TCATGA | 1 cut(s) | 1414 |
| PaqCI | CACCTGC | 1 cut(s) | 1130 |
| PciI | ACATGT | 1 cut(s) | 54 |
| PciSI | GCTCTTC | 1 cut(s) | 1752 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 102, 105 |
| PdiI | GCCGGC | 1 cut(s) | 183 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1225 |
| PfeI | GAWTC | 8 cut(s) | 43, 542, 908, 1304, 1337, 1343, 1692, 1724 |
| PfoI | TCCNGGA | 1 cut(s) | 831 |
| PinAI | ACCGGT | 1 cut(s) | 1671 |
| Ple19I | CGATCG | 1 cut(s) | 264 |
| PleI | GAGTC | 3 cut(s) | 205, 238, 460 |
| PluTI | GGCGCC | 1 cut(s) | 182 |
| PmaCI | CACGTG | 2 cut(s) | 189, 1052 |
| PmlI | CACGTG | 2 cut(s) | 189, 1052 |
| PpsI | GAGTC | 3 cut(s) | 205, 238, 460 |
| Ppu21I | YACGTR | 5 cut(s) | 189, 533, 569, 1052, 1784 |
| PscI | ACATGT | 1 cut(s) | 54 |
| Psp6I | CCWGG | 3 cut(s) | 951, 1124, 1684 |
| PspCI | CACGTG | 2 cut(s) | 189, 1052 |
| PspFI | CCCAGC | 1 cut(s) | 877 |
| PspGI | CCWGG | 3 cut(s) | 951, 1124, 1684 |
| PspN4I | GGNNCC | 3 cut(s) | 180, 621, 957 |
| PspPI | GGNCC | 2 cut(s) | 619, 1748 |
| PstI | CTGCAG | 2 cut(s) | 703, 1582 |
| PstNI | CAGNNNCTG | 3 cut(s) | 911, 977, 1082 |
| PsuI | RGATCY | 1 cut(s) | 1459 |
| PvuI | CGATCG | 1 cut(s) | 264 |
| PvuII | CAGCTG | 2 cut(s) | 809, 881 |
| RsaI | GTAC | 7 cut(s) | 50, 574, 727, 805, 859, 1171, 1796 |
| RsaNI | GTAC | 7 cut(s) | 49, 573, 726, 804, 858, 1170, 1795 |
| RseI | CAYNNNNRTG | 2 cut(s) | 648, 1050 |
| SapI | GCTCTTC | 1 cut(s) | 1752 |
| SaqAI | TTAA | 6 cut(s) | 285, 395, 741, 818, 1019, 1584 |
| Sau3AI | GATC | 8 cut(s) | 140, 192, 261, 1033, 1406, 1459, 1824, 1831 |
| Sau96I | GGNCC | 2 cut(s) | 619, 1748 |
| ScaI | AGTACT | 3 cut(s) | 727, 859, 1171 |
| SchI | GAGTC | 3 cut(s) | 206, 238, 461 |
| ScrFI | CCNGG | 4 cut(s) | 833, 953, 1126, 1686 |
| SduI | GDGCHC | 2 cut(s) | 1147, 1261 |
| SexAI | ACCWGGT | 1 cut(s) | 951 |
| SfcI | CTRYAG | 3 cut(s) | 699, 1107, 1578 |
| SfoI | GGCGCC | 1 cut(s) | 180 |
| SinI | GGWCC | 1 cut(s) | 1748 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 648, 1050 |
| SmlI | CTYRAG | 1 cut(s) | 740 |
| SmoI | CTYRAG | 1 cut(s) | 740 |
| Sse9I | AATT | 7 cut(s) | 310, 385, 525, 845, 1384, 1499, 1865 |
| SspDI | GGCGCC | 1 cut(s) | 178 |
| SspMI | CTAG | 6 cut(s) | 147, 248, 671, 1113, 1208, 1815 |
| StyD4I | CCNGG | 4 cut(s) | 831, 951, 1124, 1684 |
| StyI | CCWWGG | 3 cut(s) | 495, 670, 1296 |
| TaaI | ACNGT | 5 cut(s) | 435, 686, 772, 1794, 1799 |
| TaiI | ACGT | 6 cut(s) | 191, 535, 571, 1054, 1264, 1786 |
| TaqI | TCGA | 4 cut(s) | 77, 137, 195, 1479 |
| TaqII | GACCGA | 1 cut(s) | 1765 |
| TasI | AATT | 7 cut(s) | 310, 385, 525, 845, 1384, 1499, 1865 |
| TatI | WGTACW | 5 cut(s) | 48, 725, 857, 1169, 1794 |
| TauI | GCSGC | 3 cut(s) | 8, 29, 605 |
| TfiI | GAWTC | 8 cut(s) | 43, 542, 908, 1304, 1337, 1343, 1692, 1724 |
| Tru1I | TTAA | 6 cut(s) | 285, 395, 741, 818, 1019, 1584 |
| Tru9I | TTAA | 6 cut(s) | 285, 395, 741, 818, 1019, 1584 |
| TscAI | CASTG | 4 cut(s) | 621, 689, 798, 1455 |
| TseFI | GTSAC | 1 cut(s) | 1000 |
| Tsp45I | GTSAC | 1 cut(s) | 1000 |
| TspRI | CASTG | 4 cut(s) | 621, 689, 798, 1455 |
| Vha464I | CTTAAG | 1 cut(s) | 740 |
| VneI | GTGCAC | 2 cut(s) | 1143, 1257 |
| VpaK11BI | GGWCC | 1 cut(s) | 1748 |
| XapI | RAATTY | 1 cut(s) | 310 |
| XbaI | TCTAGA | 1 cut(s) | 1814 |
| XceI | RCATGY | 2 cut(s) | 58, 1766 |
| XmaJI | CCTAGG | 1 cut(s) | 670 |
| XmiI | GTMKAC | 2 cut(s) | 767, 1099 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1225 |
| XspI | CTAG | 6 cut(s) | 147, 248, 671, 1113, 1208, 1815 |
| ZrmI | AGTACT | 3 cut(s) | 727, 859, 1171 |