pycom01g07530

UBA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001428
Physical Location & Seq
Forward (+)
8473043 .. 8477388
4346 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07530.4

Sequence Viewer

Length: 1497 bp
ATGGCGAGTCCAACTCAGGACATGATCGACACGTACATGAGCATCACCGGCGCGTCTCATTCACTCGCGCTTCGAAAGCTGGAGGAATACGGAGGAAATCTCAGCGAAGCCGTGAATGCACATTTTGGGGGAGTATATAGAGACTTTACAAATCTGGGGTCGGCTGCTAACCCCCAGTATAATTTCTCTCATATGGATGGCCAGAATCAGGTTGCACCACATGCTTACCCGCAATACAATAATATGAGTGCTCAGAATCAAGTTGCACCGCAAGGACTTGGACCACTTCTCTCTGCTGCTAGGAGTTTCAGGCCCTCGTTACTCCTTGATCCTAATTACAGTAGAGGTCTCTTTAATCGAATTACTGGTCGTGCACCATTAATTTCACAGCCAGGAGCGGTACCTGGGGTTCCTGTGGAGTTTAACAGAGGGAATGATCAGCCTCATCTCTCAGGACAGAGGCCTCCCATTCAGGACATGACAGGAAATCTACATGGAAATGATGTGGAGGAGGAAATGATTCGAGCTGCTATTGAGGCTTCAAAACAAGATGCTCAAAAGGCTTATCTTCTGAATATGCAAAGCAGGGCTCAAAATGTGTCTCCTGTTAATGGGCTTCCAGATAATCAAATTCACCTAGATAACAATGATTTTGATCGTGCAGTTTCACTGTCCCTGAAGACAGCAGAACGAGAGAAAGCAATGCGTGAGCAACAAATGAAAGATAAGAATCAAGTAGCAGGAACTTATAGTACATCAAGGTGGGAAACCTCAATGCACCACGATGGAGATGGAACTGTAGAAAGAAAACAAGTGAGTCAAGAGTTGAAGCATGATTCTGGTAACAATCTTCAGCAGGGTAACCTTGCCATTAGTTCTGAAGAGTTAGGCGGCATTTCTTCCAAAGAGCTTGACGAAGCTATACTGCTTGAGAATGCACTTTTTGGTGAATCCTCATATGCACCTAATTTTCCAAGTGGTCCAAGTAGAAATTTGGATCCTAATTTGCAGCCTGTTCATGGCCCTTCATCATCCGCTCTAACAACAAGACAATTGCTTAGAGAACAACAGGATTGTGAGTATCTCGCATCCCTCTTGGCTGATAAAGAAAAGGAAATGAATGGTGTTAATGAAGTTGGAACTTGTGACATAAAGGATGTTGGCTCTGACAAAAAGATTGATCCATTGGAACTCGAGAGTAGGCTTGCTGCGAAAAGGGCTTCGCTTCCTTGTGAACCAGCAATAGATGATCAGAATGCTGTGACTCTTCTTGTAAGGATGCCAAATGGAACTCGCCTTAGTCGCCGCTTTAACAAGTCTGACAAGCTTCAGGTTTTGTTCGACTTCATAGATGTTGGTAGAGAGGTTAAGCCTGGAACTTACAGAGTGGTGAGGTCATATCCTCGGCGTGCTTTTACTGTTGGTGACAGCATACTATCGTTAAGTGAACTTGGCTTGACAAATAAACAAGAAGCTCTGTTTCTGGAATTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBA_4 UBX UIM

Protein Analysis

499

Amino Acids

54.8

Weight (kDa)

5.42

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 400
AccBSI CCGCTC 2 cut(s) 398, 1037
AccII CGCG 2 cut(s) 53, 68
AciI CCGC 6 cut(s) 230, 269, 398, 891, 1035, 1306
AclWI GGATC 4 cut(s) 323, 992, 1005, 1175
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 2 cut(s) 630, 991
AcuI CTGAAG 4 cut(s) 698, 836, 900, 1313
AfaI GTAC 3 cut(s) 35, 402, 754
AfiI CCNNNNNNNGG 3 cut(s) 208, 611, 1019
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 543, 829
AjnI CCWGG 3 cut(s) 391, 403, 1372
AluBI AGCT 6 cut(s) 79, 527, 910, 920, 1327, 1475
AluI AGCT 6 cut(s) 79, 527, 910, 920, 1327, 1475
Alw21I GWGCWC 2 cut(s) 253, 376
Alw26I GTCTC 4 cut(s) 60, 135, 353, 606
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 4 cut(s) 323, 992, 1005, 1175
Ama87I CYCGRG 1 cut(s) 1193
AoxI GGCC 4 cut(s) 199, 311, 461, 1021
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 5 cut(s) 164, 296, 527, 1009, 1208
ApoI RAATTY 2 cut(s) 630, 991
ArsI GACNNNNNNTTYG 4 cut(s) 143, 175, 352, 384
AseI ATTAAT 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 519
Asp718I GGTACC 1 cut(s) 400
AspLEI GCGC 2 cut(s) 53, 70
AspS9I GGNCC 4 cut(s) 281, 312, 980, 1022
AsuHPI GGTGA 5 cut(s) 37, 626, 959, 1402, 1436
AsuII TTCGAA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 2 cut(s) 281, 980
BaeGI GKGCMC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 201
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 2 cut(s) 253, 376
BbvI GCAGC 5 cut(s) 151, 283, 514, 1021, 1195
BccI CCATC 3 cut(s) 191, 779, 785
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 3 cut(s) 393, 405, 1374
BclI TGATCA 2 cut(s) 436, 1249
BcoDI GTCTC 4 cut(s) 60, 135, 353, 606
BfaI CTAG 2 cut(s) 300, 638
BfmI CTRYAG 1 cut(s) 798
BisI GCNGC 7 cut(s) 165, 297, 528, 892, 1010, 1209, 1306
BlsI GCNGC 7 cut(s) 166, 298, 529, 893, 1011, 1210, 1307
Bme1390I CCNGG 3 cut(s) 393, 405, 1374
Bme18I GGWCC 2 cut(s) 281, 980
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 4 cut(s) 281, 312, 980, 1022
BmiI GGNNCC 3 cut(s) 402, 411, 999
BmrFI CCNGG 3 cut(s) 393, 405, 1374
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 4 cut(s) 51, 541, 1097, 1269
BmuI ACTGGG 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 3 cut(s) 30, 84, 116
BpmI CTGGAG 1 cut(s) 101
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 950
BsaAI YACGTR 1 cut(s) 33
BsaBI GATNNNNATC 2 cut(s) 654, 729
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 2 cut(s) 404, 1403
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 611, 1019
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 2 cut(s) 175, 370
Bse3DI GCAATG 1 cut(s) 708
Bse8I GATNNNNATC 2 cut(s) 654, 729
BseBI CCWGG 3 cut(s) 393, 405, 1374
BseDI CCNNGG 2 cut(s) 404, 1403
BseGI GGATG 5 cut(s) 202, 1031, 1088, 1162, 1284
BseJI GATNNNNATC 2 cut(s) 654, 729
BseLI CCNNNNNNNGG 3 cut(s) 208, 611, 1019
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 4 cut(s) 29, 115, 266, 465
BseNI ACTGG 2 cut(s) 175, 370
BseRI GAGGAG 1 cut(s) 524
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 5 cut(s) 151, 283, 514, 1021, 1195
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 2 cut(s) 53, 68
BshFI GGCC 4 cut(s) 201, 313, 463, 1023
BshNI GGYRCC 1 cut(s) 400
BsiHKAI GWGCWC 2 cut(s) 253, 376
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 3 cut(s) 208, 611, 1019
BsmAI GTCTC 4 cut(s) 60, 135, 353, 606
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 3 cut(s) 121, 940, 1261
BsnI GGCC 4 cut(s) 201, 313, 463, 1023
Bso31I GGTCTC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 1193
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 3 cut(s) 253, 376, 592
Bsp143I GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
BspACI CCGC 6 cut(s) 230, 269, 398, 891, 1035, 1306
BspANI GGCC 4 cut(s) 201, 313, 463, 1023
BspCNI CTCAG 4 cut(s) 28, 114, 265, 464
BspFNI CGCG 2 cut(s) 53, 68
BspLI GGNNCC 3 cut(s) 402, 411, 999
BspPI GGATC 4 cut(s) 323, 992, 1005, 1175
BspT104I TTCGAA 1 cut(s) 73
BspT107I GGYRCC 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 353
BsrBI CCGCTC 2 cut(s) 398, 1037
BsrDI GCAATG 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 2 cut(s) 175, 370
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 404, 1403
BssMI GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
Bst2UI CCWGG 3 cut(s) 393, 405, 1374
Bst4CI ACNGT 4 cut(s) 341, 672, 799, 1420
Bst6I CTCTTC 2 cut(s) 876, 1272
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 33
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 1206, 1410
BstDEI CTNAG 6 cut(s) 15, 101, 252, 451, 1058, 1298
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 5 cut(s) 202, 1031, 1088, 1162, 1284
BstFNI CGCG 2 cut(s) 53, 68
BstHHI GCGC 2 cut(s) 53, 70
BstKTI GATC 7 cut(s) 27, 331, 439, 658, 1000, 1183, 1252
BstMAI GTCTC 4 cut(s) 60, 135, 353, 606
BstMBI GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
BstMWI GCNNNNNNNGC 8 cut(s) 48, 76, 116, 221, 536, 560, 1217, 1302
BstNI CCWGG 3 cut(s) 393, 405, 1374
BstNSI RCATGY 1 cut(s) 224
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 391, 403, 1372
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 2 cut(s) 53, 68
BstV1I GCAGC 5 cut(s) 151, 283, 514, 1021, 1195
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 1 cut(s) 997
BstYI RGATCY 1 cut(s) 997
BsuRI GGCC 4 cut(s) 201, 313, 463, 1023
BtsCI GGATG 5 cut(s) 202, 1031, 1088, 1162, 1284
BtsIMutI CAGTG 1 cut(s) 668
Cac8I GCNNGC 2 cut(s) 1206, 1410
CfoI GCGC 2 cut(s) 53, 70
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 4 cut(s) 281, 312, 980, 1022
CseI GACGC 1 cut(s) 42
Csp6I GTAC 3 cut(s) 34, 401, 753
CviAII CATG 7 cut(s) 22, 37, 221, 478, 494, 833, 1019
CviQI GTAC 3 cut(s) 34, 401, 753
DdeI CTNAG 6 cut(s) 15, 101, 252, 451, 1058, 1298
DpnI GATC 7 cut(s) 26, 330, 438, 657, 999, 1182, 1251
DpnII GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 2 cut(s) 876, 1272
EarI CTCTTC 2 cut(s) 876, 1272
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 592
Eco31I GGTCTC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 281, 980
Eco57I CTGAAG 4 cut(s) 698, 836, 900, 1313
Eco88I CYCGRG 1 cut(s) 1193
Eco91I GGTNACC 1 cut(s) 860
EcoO109I RGGNCCY 1 cut(s) 312
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 391, 403, 1372
EcoT38I GRGCYC 1 cut(s) 592
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 7 cut(s) 25, 40, 224, 481, 497, 836, 1022
FaqI GGGAC 1 cut(s) 658
FatI CATG 7 cut(s) 21, 36, 220, 477, 493, 832, 1018
FauI CCCGC 1 cut(s) 237
FauNDI CATATG 2 cut(s) 192, 958
FbaI TGATCA 2 cut(s) 436, 1249
Fnu4HI GCNGC 7 cut(s) 165, 297, 528, 892, 1010, 1209, 1306
FokI GGATG 5 cut(s) 209, 1018, 1075, 1169, 1291
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 7 cut(s) 165, 297, 528, 892, 1010, 1209, 1306
FspBI CTAG 2 cut(s) 300, 638
GlaI GCGC 2 cut(s) 52, 69
GluI GCNGC 7 cut(s) 165, 297, 528, 892, 1010, 1209, 1306
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 4 cut(s) 201, 313, 463, 1023
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 42
HhaI GCGC 2 cut(s) 53, 70
Hin1II CATG 7 cut(s) 25, 40, 224, 481, 497, 836, 1022
Hin6I GCGC 2 cut(s) 51, 68
HinP1I GCGC 2 cut(s) 51, 68
HindIII AAGCTT 1 cut(s) 1325
HinfI GANTC 9 cut(s) 7, 205, 256, 520, 730, 817, 836, 950, 1264
HpaII CCGG 1 cut(s) 48
HphI GGTGA 5 cut(s) 37, 626, 959, 1402, 1436
Hpy166II GTNNAC 3 cut(s) 374, 1235, 1448
Hpy188I TCNGA 6 cut(s) 255, 573, 880, 1168, 1254, 1321
Hpy188III TCNNGA 7 cut(s) 17, 453, 473, 620, 821, 1195, 1484
Hpy8I GTNNAC 3 cut(s) 374, 1235, 1448
HpyAV CCTTC 1 cut(s) 1035
HpyCH4III ACNGT 4 cut(s) 341, 672, 799, 1420
HpyCH4IV ACGT 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 76, 116, 221, 536, 560, 1217, 1302
HpyF3I CTNAG 6 cut(s) 15, 101, 252, 451, 1058, 1298
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 7 cut(s) 25, 40, 224, 481, 497, 836, 1022
HspAI GCGC 2 cut(s) 51, 68
KpnI GGTACC 1 cut(s) 404
Ksp22I TGATCA 2 cut(s) 436, 1249
Kzo9I GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 5 cut(s) 151, 283, 514, 1021, 1195
LweI GCATC 4 cut(s) 51, 541, 1097, 1269
MaeI CTAG 2 cut(s) 300, 638
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 6 cut(s) 318, 842, 860, 1145, 1261, 1424
MalI GATC 7 cut(s) 26, 330, 438, 657, 999, 1182, 1251
MbiI CCGCTC 2 cut(s) 398, 1037
MboI GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
MboII GAAGA 6 cut(s) 560, 691, 842, 891, 893, 1259
MfeI CAATTG 1 cut(s) 1052
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 3 cut(s) 253, 376, 592
MlsI TGGCCA 1 cut(s) 201
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 3 cut(s) 16, 826, 1258
MmeI TCCRAC 2 cut(s) 35, 1117
Mox20I TGGCCA 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 201
MseI TTAA 8 cut(s) 354, 380, 423, 609, 1128, 1311, 1368, 1442
MslI CAYNNNNRTG 5 cut(s) 35, 195, 498, 760, 783
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 3 cut(s) 393, 405, 1374
MunI CAATTG 1 cut(s) 1052
Mva1269I GAATGC 3 cut(s) 121, 940, 1261
MvaI CCWGG 3 cut(s) 393, 405, 1374
MvnI CGCG 2 cut(s) 53, 68
MwoI GCNNNNNNNGC 8 cut(s) 48, 76, 116, 221, 536, 560, 1217, 1302
NdeI CATATG 2 cut(s) 192, 958
NdeII GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
NlaIII CATG 7 cut(s) 25, 40, 224, 481, 497, 836, 1022
NlaIV GGNNCC 3 cut(s) 402, 411, 999
NmeAIII GCCGAG 1 cut(s) 1384
NmuCI GTSAC 3 cut(s) 1145, 1261, 1424
NspI RCATGY 1 cut(s) 224
NspV TTCGAA 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 1193
PceI AGGCCT 1 cut(s) 463
PctI GAATGC 3 cut(s) 121, 940, 1261
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 6 cut(s) 205, 256, 520, 730, 836, 950
PkrI GCNGC 7 cut(s) 166, 298, 529, 893, 1011, 1210, 1307
PleI GAGTC 3 cut(s) 15, 825, 1258
PpsI GAGTC 3 cut(s) 15, 825, 1258
Ppu21I YACGTR 1 cut(s) 33
PshBI ATTAAT 1 cut(s) 380
Psp6I CCWGG 3 cut(s) 391, 403, 1372
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 3 cut(s) 391, 403, 1372
PspN4I GGNNCC 3 cut(s) 402, 411, 999
PspPI GGNCC 4 cut(s) 281, 312, 980, 1022
PsrI GAACNNNNNNTAC 4 cut(s) 393, 425, 736, 768
PsuI RGATCY 1 cut(s) 997
RsaI GTAC 3 cut(s) 35, 402, 754
RsaNI GTAC 3 cut(s) 34, 401, 753
RseI CAYNNNNRTG 5 cut(s) 35, 195, 498, 760, 783
SaqAI TTAA 8 cut(s) 354, 380, 423, 609, 1128, 1311, 1368, 1442
SatI GCNGC 7 cut(s) 165, 297, 528, 892, 1010, 1209, 1306
Sau3AI GATC 7 cut(s) 24, 328, 436, 655, 997, 1180, 1249
Sau96I GGNCC 4 cut(s) 281, 312, 980, 1022
SchI GAGTC 3 cut(s) 16, 826, 1258
ScrFI CCNGG 3 cut(s) 393, 405, 1374
SduI GDGCHC 3 cut(s) 253, 376, 592
SfaNI GCATC 4 cut(s) 51, 541, 1097, 1269
SfcI CTRYAG 1 cut(s) 798
Sfr274I CTCGAG 1 cut(s) 1193
SfuI TTCGAA 1 cut(s) 73
SgrAI CRCCGGYG 1 cut(s) 47
SinI GGWCC 2 cut(s) 281, 980
SlaI CTCGAG 1 cut(s) 1193
SmiMI CAYNNNNRTG 5 cut(s) 35, 195, 498, 760, 783
SmlI CTYRAG 2 cut(s) 929, 1193
SmoI CTYRAG 2 cut(s) 929, 1193
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 6 cut(s) 230, 269, 398, 891, 1035, 1306
SspMI CTAG 2 cut(s) 300, 638
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 3 cut(s) 391, 403, 1372
TaaI ACNGT 4 cut(s) 341, 672, 799, 1420
TaiI ACGT 1 cut(s) 35
TaqI TCGA 6 cut(s) 27, 73, 358, 523, 1194, 1341
TatI WGTACW 1 cut(s) 752
TauI GCSGC 2 cut(s) 894, 1308
TfiI GAWTC 6 cut(s) 205, 256, 520, 730, 836, 950
Tru1I TTAA 8 cut(s) 354, 380, 423, 609, 1128, 1311, 1368, 1442
Tru9I TTAA 8 cut(s) 354, 380, 423, 609, 1128, 1311, 1368, 1442
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 3 cut(s) 1145, 1261, 1424
TseI GCWGC 5 cut(s) 164, 296, 527, 1009, 1208
Tsp45I GTSAC 3 cut(s) 1145, 1261, 1424
TspDTI ATGAA 6 cut(s) 734, 1007, 1017, 1133, 1146, 1336
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 675
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 2 cut(s) 281, 980
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 2 cut(s) 630, 991
XceI RCATGY 1 cut(s) 224
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
XhoI CTCGAG 1 cut(s) 1193
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 300, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.