Rroxscaffold_4G00302630

UBA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001428
Physical Location & Seq
Forward (+)
22958785 .. 22964130
5346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302630.1

Sequence Viewer

Length: 1434 bp
ATGCCGAGGCCAACTGAGGACAAGATCCAGACCTACATCAGCCTCACCGGCGCGTCTCACTCACTCGCGCTGCTAAAGCTCGAGGAACATGGAGGAGATCTCAATGAAGCCGTGAATGCACATTTTAGGGAAGGAGATGTACACAGCACAAGTCCTTCGTCTACTGCTCCCCCACAGTACAATTTCCCTCAAATGAGTAATCAGAATCAAGTAGCACCACAACAAGGACTTCTGCCACTTCTCTCTGTTGCCAGGAGTTTCAAGCCTTCATTACTACTTGACCGTAGTTACAGTAGAGATCTCTACAATCGGATTGGTTCTGCATTAACTGGTCGTGCACCATCACCTTCACACCCAGGAGAGGTAACTGGGGTTCCTGTAGGTTTCAATGCCGGGAATGAGCAGCCTTATCTCTCAGGACAGAGGCCTACCATTCCGGATGTGGCAAGAACTGACTTGGAGGCCTCAAGATATGGCAATGATGTGGAGGAAGAAATGATTCAAGCTGCTATTGAAGCTTCAAAACGAGAAGCTGAAATTGGTTCTCCGAATGTACGAAACAGTACTCCCAATGTATCTCCTGGTAATGGGCTTCCGGTTGAGAAAATTCCTGAAGAAAATTCAGATTTTGATCGTGCAGTTTCGTTGTCCTTGAAGACAGCAGAACAAGAGAAAGCAATACGTGAGATGCAGCTGAACGACAGGAATCAAGATCCAGGAGTTTATAGTACATGGAAGCAAGGAAGGTCATCACAGCAAAATGGAGCTGAAGTTGTAGAGCAGCAATTAGTTAGTGAAGAGTCTAAGCGTGATGGTGGTAGTCATCTTCAGGACGGAAAACTGGCAACTCTCAGTGATGAGTTGGGAGGCTTATCTCCAAAAGAGCTTGACGAAGCAATTATGCTTGAGACTGCACTTTTTGGTAAATCTCCATACACTCCTAATAATCAGCCTATACATGGCTCTTCCTCATCCTCTCTATCAGCAAGGCAATTGCTCAGAGAACAACAGGATGATGAGTATCTTGCATCCCTCTTAGCTGATAAAGAGAAAGAAATGAATGCTGTCCATGACGCTGCAGCTTGTCAGTTGAAGGGAGATGTCAACAAAAGAATCGAGCCAATGGAATTTGAGAGAAGGCTAGATGCAAAAAGTGCTTCACTTCCTTGTGAACCAGCACCAGATGATGAGAATGCTGTGACTCTTCTTGTTCGGATGCCAAATGGCAGCCGCCTTAGCCGTCGCTTTCGCAAATCTGACAAGCTTCAGCTTCTTTTTGACTTCATAGATGTTGGTAGAGTGGTGGAGCCTGGAACTTACAGAGTGGTGAGGTCATATCCTCGGCATGCTTTCACCGCCAATAACAGCTTATTAACATTGAGAGAACTTGGCCTGACCAACAAGCAAGAAGCATTATTTTTGGAACTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBA_4 UBX UIM

Protein Analysis

477

Amino Acids

52.48

Weight (kDa)

5.18

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_4 PF14555 6 - 46 6.7e-09 UBA-like domain
UBX PF00789 396 - 475 1.8e-17 UBX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AccII CGCG 2 cut(s) 53, 68
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 2 cut(s) 1231, 1356
AclWI GGATC 2 cut(s) 19, 707
AcsI RAATTY 3 cut(s) 606, 619, 1127
AcuI CTGAAG 4 cut(s) 633, 789, 812, 1250
AfaI GTAC 5 cut(s) 141, 179, 555, 565, 730
AfiI CCNNNNNNNGG 4 cut(s) 224, 361, 587, 959
AgsI TTSAA 7 cut(s) 262, 388, 503, 515, 522, 655, 1093
AjnI CCWGG 5 cut(s) 251, 355, 580, 715, 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 60, 902
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 2 cut(s) 19, 707
Ama87I CYCGRG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 4 cut(s) 8, 425, 462, 1390
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 8 cut(s) 70, 403, 506, 691, 781, 1076, 1079, 1227
ApoI RAATTY 3 cut(s) 606, 619, 1127
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 2 cut(s) 53, 70
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 4 cut(s) 37, 336, 1339, 1345
AvaI CYCGRG 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 340
BarI GAAGNNNNNNTAC 2 cut(s) 123, 155
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 8 cut(s) 57, 415, 493, 703, 793, 1063, 1091, 1239
BccI CCATC 2 cut(s) 349, 806
BceAI ACGGC 2 cut(s) 95, 1224
BciT130I CCWGG 5 cut(s) 253, 357, 582, 717, 1311
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 60, 902
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 2 cut(s) 378, 1077
BglI GCCNNNNNGGC 1 cut(s) 48
BglII AGATCT 2 cut(s) 97, 298
BisI GCNGC 9 cut(s) 71, 404, 507, 692, 782, 1077, 1080, 1228, 1231
BlsI GCNGC 9 cut(s) 72, 405, 508, 693, 783, 1078, 1081, 1229, 1232
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 6 cut(s) 253, 357, 394, 582, 717, 1311
BmeT110I CYCGRG 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 375, 1308
BmrFI CCNGG 6 cut(s) 253, 357, 394, 582, 717, 1311
BmrI ACTGGG 1 cut(s) 378
BmsI GCATC 4 cut(s) 678, 1037, 1135, 1206
BmuI ACTGGG 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 662
BplI GAGNNNNNCTC 2 cut(s) 84, 116
Bpu10I CCTNAGC 1 cut(s) 1235
BpuEI CTTGAG 2 cut(s) 451, 926
BpuMI CCSGG 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 683
BsaBI GATNNNNATC 2 cut(s) 630, 1020
BsaJI CCNNGG 3 cut(s) 5, 355, 1340
BsaWI WCCGGW 2 cut(s) 436, 595
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 361, 587, 959
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 3 cut(s) 334, 373, 846
Bse3DI GCAATG 1 cut(s) 484
Bse8I GATNNNNATC 2 cut(s) 630, 1020
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 5 cut(s) 253, 357, 582, 717, 1311
BseDI CCNNGG 3 cut(s) 5, 355, 1340
BseGI GGATG 5 cut(s) 445, 971, 1018, 1028, 1221
BseJI GATNNNNATC 2 cut(s) 630, 1020
BseLI CCNNNNNNNGG 4 cut(s) 224, 361, 587, 959
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 4 cut(s) 6, 429, 865, 1012
BseNI ACTGG 3 cut(s) 334, 373, 846
BseRI GAGGAG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 8 cut(s) 57, 415, 493, 703, 793, 1063, 1091, 1239
BsgI GTGCAG 2 cut(s) 657, 897
Bsh1236I CGCG 2 cut(s) 53, 68
BshFI GGCC 4 cut(s) 10, 427, 464, 1392
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 4 cut(s) 48, 393, 437, 596
BslI CCNNNNNNNGG 4 cut(s) 224, 361, 587, 959
BsmAI GTCTC 2 cut(s) 60, 902
BsmBI CGTCTC 1 cut(s) 60
BsmI GAATGC 3 cut(s) 121, 1066, 1198
BsnI GGCC 4 cut(s) 10, 427, 464, 1392
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 340
Bsp13I TCCGGA 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 139
Bsp143I GATC 5 cut(s) 24, 97, 298, 631, 712
BspACI CCGC 2 cut(s) 1231, 1356
BspANI GGCC 4 cut(s) 10, 427, 464, 1392
BspCNI CTCAG 4 cut(s) 7, 428, 864, 1011
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 2 cut(s) 53, 68
BspLI GGNNCC 2 cut(s) 375, 1308
BspMAI CTGCAG 1 cut(s) 1081
BspPI GGATC 2 cut(s) 19, 707
BspQI GCTCTTC 1 cut(s) 970
BsrDI GCAATG 1 cut(s) 484
BsrFI RCCGGY 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 139
BsrI ACTGG 3 cut(s) 334, 373, 846
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 5, 355, 1340
BssMI GATC 5 cut(s) 24, 97, 298, 631, 712
Bst2UI CCWGG 5 cut(s) 253, 357, 582, 717, 1311
Bst4CI ACNGT 4 cut(s) 177, 284, 293, 563
Bst6I CTCTTC 3 cut(s) 792, 970, 1209
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 139
BstBAI YACGTR 1 cut(s) 683
BstC8I GCNNGC 1 cut(s) 1347
BstDEI CTNAG 7 cut(s) 15, 415, 804, 851, 998, 1036, 1235
BstF5I GGATG 5 cut(s) 445, 971, 1018, 1028, 1221
BstFNI CGCG 2 cut(s) 53, 68
BstHHI GCGC 2 cut(s) 53, 70
BstKTI GATC 5 cut(s) 27, 100, 301, 634, 715
BstMAI GTCTC 2 cut(s) 60, 902
BstMBI GATC 5 cut(s) 24, 97, 298, 631, 712
BstMWI GCNNNNNNNGC 7 cut(s) 48, 76, 116, 515, 1154, 1236, 1355
BstNI CCWGG 5 cut(s) 253, 357, 582, 717, 1311
BstNSI RCATGY 1 cut(s) 1349
BstSCI CCNGG 6 cut(s) 251, 355, 392, 580, 715, 1309
BstSFI CTRYAG 2 cut(s) 378, 1077
BstSLI GKGCMC 1 cut(s) 340
BstUI CGCG 2 cut(s) 53, 68
BstV1I GCAGC 8 cut(s) 57, 415, 493, 703, 793, 1063, 1091, 1239
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 4 cut(s) 24, 97, 298, 712
BstYI RGATCY 4 cut(s) 24, 97, 298, 712
BsuRI GGCC 4 cut(s) 10, 427, 464, 1392
BtsCI GGATG 5 cut(s) 445, 971, 1018, 1028, 1221
BtsIMutI CAGTG 1 cut(s) 859
Cac8I GCNNGC 1 cut(s) 1347
CfoI GCGC 2 cut(s) 53, 70
Cfr10I RCCGGY 1 cut(s) 47
CseI GACGC 2 cut(s) 42, 1082
Csp6I GTAC 5 cut(s) 140, 178, 554, 564, 729
CviAII CATG 5 cut(s) 89, 732, 959, 1070, 1346
CviQI GTAC 5 cut(s) 140, 178, 554, 564, 729
DdeI CTNAG 7 cut(s) 15, 415, 804, 851, 998, 1036, 1235
DpnI GATC 5 cut(s) 26, 99, 300, 633, 714
DpnII GATC 5 cut(s) 24, 97, 298, 631, 712
Eam1104I CTCTTC 3 cut(s) 792, 970, 1209
EarI CTCTTC 3 cut(s) 792, 970, 1209
Eco147I AGGCCT 2 cut(s) 427, 464
Eco57I CTGAAG 4 cut(s) 633, 789, 812, 1250
Eco88I CYCGRG 1 cut(s) 80
EcoRII CCWGG 5 cut(s) 251, 355, 580, 715, 1309
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 5 cut(s) 92, 735, 962, 1073, 1349
FatI CATG 5 cut(s) 88, 731, 958, 1069, 1345
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 9 cut(s) 71, 404, 507, 692, 782, 1077, 1080, 1228, 1231
FokI GGATG 5 cut(s) 452, 958, 1015, 1025, 1228
Fsp4HI GCNGC 9 cut(s) 71, 404, 507, 692, 782, 1077, 1080, 1228, 1231
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 2 cut(s) 52, 69
GluI GCNGC 9 cut(s) 71, 404, 507, 692, 782, 1077, 1080, 1228, 1231
HaeIII GGCC 4 cut(s) 10, 427, 464, 1392
HapII CCGG 4 cut(s) 48, 393, 437, 596
HgaI GACGC 2 cut(s) 42, 1082
HhaI GCGC 2 cut(s) 53, 70
Hin1II CATG 5 cut(s) 92, 735, 962, 1073, 1349
Hin6I GCGC 2 cut(s) 51, 68
HinP1I GCGC 2 cut(s) 51, 68
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 2 cut(s) 516, 1262
HinfI GANTC 6 cut(s) 205, 499, 706, 800, 1113, 1201
HpaII CCGG 4 cut(s) 48, 393, 437, 596
HphI GGTGA 4 cut(s) 37, 336, 1339, 1345
Hpy166II GTNNAC 5 cut(s) 142, 162, 338, 1105, 1172
Hpy188I TCNGA 7 cut(s) 204, 312, 549, 625, 1001, 1215, 1258
Hpy188III TCNNGA 7 cut(s) 28, 417, 437, 468, 611, 710, 830
Hpy8I GTNNAC 5 cut(s) 142, 162, 338, 1105, 1172
Hpy99I CGWCG 1 cut(s) 1245
HpyAV CCTTC 7 cut(s) 125, 165, 276, 357, 738, 1087, 1131
HpyCH4III ACNGT 4 cut(s) 177, 284, 293, 563
HpyCH4IV ACGT 1 cut(s) 682
HpyCH4V TGCA 9 cut(s) 119, 323, 338, 638, 691, 914, 1028, 1079, 1148
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 76, 116, 515, 1154, 1236, 1355
HpyF3I CTNAG 7 cut(s) 15, 415, 804, 851, 998, 1036, 1235
HpySE526I ACGT 1 cut(s) 682
Hsp92II CATG 5 cut(s) 92, 735, 962, 1073, 1349
HspAI GCGC 2 cut(s) 51, 68
Kpn2I TCCGGA 1 cut(s) 436
Kzo9I GATC 5 cut(s) 24, 97, 298, 631, 712
LguI GCTCTTC 1 cut(s) 970
LmnI GCTCC 3 cut(s) 172, 764, 1306
Lsp1109I GCAGC 8 cut(s) 57, 415, 493, 703, 793, 1063, 1091, 1239
LweI GCATC 4 cut(s) 678, 1037, 1135, 1206
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 1 cut(s) 682
MaeIII GTNAC 3 cut(s) 287, 364, 1198
MalI GATC 5 cut(s) 26, 99, 300, 633, 714
MboI GATC 5 cut(s) 24, 97, 298, 631, 712
MboII GAAGA 7 cut(s) 503, 626, 667, 809, 818, 957, 1196
MfeI CAATTG 1 cut(s) 992
MflI RGATCY 4 cut(s) 24, 97, 298, 712
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 8 cut(s) 181, 537, 606, 619, 785, 897, 992, 1127
MlyI GAGTC 2 cut(s) 809, 1195
MroI TCCGGA 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 3 cut(s) 326, 1373, 1432
MspA1I CMGCKG 1 cut(s) 694
MspI CCGG 4 cut(s) 48, 393, 437, 596
MspR9I CCNGG 6 cut(s) 253, 357, 394, 582, 717, 1311
MunI CAATTG 1 cut(s) 992
Mva1269I GAATGC 3 cut(s) 121, 1066, 1198
MvaI CCWGG 5 cut(s) 253, 357, 582, 717, 1311
MvnI CGCG 2 cut(s) 53, 68
MwoI GCNNNNNNNGC 7 cut(s) 48, 76, 116, 515, 1154, 1236, 1355
NciI CCSGG 1 cut(s) 394
NdeII GATC 5 cut(s) 24, 97, 298, 631, 712
NlaIII CATG 5 cut(s) 92, 735, 962, 1073, 1349
NlaIV GGNNCC 2 cut(s) 375, 1308
NmeAIII GCCGAG 2 cut(s) 30, 1321
NmuCI GTSAC 1 cut(s) 1198
NspI RCATGY 1 cut(s) 1349
PaeI GCATGC 1 cut(s) 1349
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 2 cut(s) 427, 464
PciSI GCTCTTC 1 cut(s) 970
PctI GAATGC 3 cut(s) 121, 1066, 1198
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 4 cut(s) 205, 499, 706, 1113
PfoI TCCNGGA 1 cut(s) 715
PkrI GCNGC 9 cut(s) 72, 405, 508, 693, 783, 1078, 1081, 1229, 1232
PleI GAGTC 2 cut(s) 808, 1195
PpsI GAGTC 2 cut(s) 808, 1195
Ppu21I YACGTR 1 cut(s) 683
Psp6I CCWGG 5 cut(s) 251, 355, 580, 715, 1309
PspGI CCWGG 5 cut(s) 251, 355, 580, 715, 1309
PspN4I GGNNCC 2 cut(s) 375, 1308
PspXI VCTCGAGB 1 cut(s) 80
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PstI CTGCAG 1 cut(s) 1081
PsuI RGATCY 4 cut(s) 24, 97, 298, 712
PvuII CAGCTG 1 cut(s) 694
RsaI GTAC 5 cut(s) 141, 179, 555, 565, 730
RsaNI GTAC 5 cut(s) 140, 178, 554, 564, 729
SapI GCTCTTC 1 cut(s) 970
SaqAI TTAA 3 cut(s) 326, 1373, 1432
SatI GCNGC 9 cut(s) 71, 404, 507, 692, 782, 1077, 1080, 1228, 1231
Sau3AI GATC 5 cut(s) 24, 97, 298, 631, 712
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 2 cut(s) 809, 1195
ScrFI CCNGG 6 cut(s) 253, 357, 394, 582, 717, 1311
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 4 cut(s) 678, 1037, 1135, 1206
SfcI CTRYAG 2 cut(s) 378, 1077
Sfr274I CTCGAG 1 cut(s) 80
SgrAI CRCCGGYG 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 3 cut(s) 80, 466, 905
SmoI CTYRAG 3 cut(s) 80, 466, 905
SphI GCATGC 1 cut(s) 1349
Sse9I AATT 8 cut(s) 181, 537, 606, 619, 785, 897, 992, 1127
SseBI AGGCCT 2 cut(s) 427, 464
SsiI CCGC 2 cut(s) 1231, 1356
SspMI CTAG 1 cut(s) 1142
StuI AGGCCT 2 cut(s) 427, 464
StyD4I CCNGG 6 cut(s) 251, 355, 392, 580, 715, 1309
TaaI ACNGT 4 cut(s) 177, 284, 293, 563
TaiI ACGT 1 cut(s) 685
TaqI TCGA 2 cut(s) 81, 1116
TasI AATT 8 cut(s) 181, 537, 606, 619, 785, 897, 992, 1127
TatI WGTACW 4 cut(s) 139, 177, 563, 728
TauI GCSGC 1 cut(s) 1233
TfiI GAWTC 4 cut(s) 205, 499, 706, 1113
Tru1I TTAA 3 cut(s) 326, 1373, 1432
Tru9I TTAA 3 cut(s) 326, 1373, 1432
TscAI CASTG 1 cut(s) 859
TseFI GTSAC 1 cut(s) 1198
TseI GCWGC 8 cut(s) 70, 403, 506, 691, 781, 1076, 1079, 1227
Tsp45I GTSAC 1 cut(s) 1198
TspDTI ATGAA 4 cut(s) 120, 258, 1073, 1273
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 1 cut(s) 859
VneI GTGCAC 1 cut(s) 336
XapI RAATTY 3 cut(s) 606, 619, 1127
XceI RCATGY 1 cut(s) 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 1142
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.